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南海海滩岩微生物群落结构和特定菌株对其成因机制的启示

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热带海洋学报 2024年第4期43卷 112-122页

作者：刘玓玓张喜洋孙富林王明壮谭飞施祺王冠杨红强中国科学院边缘海与大洋地质重点实验室中国科学院南海海洋研究所广东广州510301 海洋学院华南农业大学广东广州510642 南方海洋科学与工程广东省实验室(广州) 广东广州511458 中国科学院热带海洋生物资源与生态重点实验室中国科学院南海海洋研究所广东广州510301 中国科学院大学北京100049

热带—亚热带潮间带沉积物中丰富的菌—藻类微生物及其复杂的生命活动对颗粒的早期胶结过程起重要作用。本文通过16S r RNA高通量基因测序方法分析西沙群岛石屿、全富岛及海南龙湾珊瑚礁滨岸带海滩岩表生微生物群落结构,确定主导海滩岩... 详细信息

热带—亚热带潮间带沉积物中丰富的菌—藻类微生物及其复杂的生命活动对颗粒的早期胶结过程起重要作用。本文通过16S r RNA高通量基因测序方法分析西沙群岛石屿、全富岛及海南龙湾珊瑚礁滨岸带海滩岩表生微生物群落结构,确定主导海滩岩快速胶结的共性菌株/微生物功能群。表层生长草皮海藻的全富岛海滩岩原核生物多样性最高。龙湾潮间带上部暴露时间较长的海滩岩比潮间带下部海滩岩原核生物多样性高。相对固结的石屿海滩岩原核生物多样性最低。4个取样点具有较为相似的细菌门类组成,物种丰度由大到小依次为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteriota)、蓝细菌门(Cyanobacteria)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadota)和脱硫杆菌门(Desulfobacterota)。不同点位的海滩岩形成环境中蓝细菌主要种类大体相同,其中眉藻(Calothrixsp.)是主导海滩岩早期胶结的共性钙化菌株,通过分泌胞外聚合物成为碳酸盐矿物沉淀的“热点”。群落中检出的反硝化细菌和硫酸盐还原菌表明海滩岩内部氧化还原梯度较大,表面下极浅处即存在缺氧微环境。微生物群落内强烈的光合作用、硫酸盐还原作用和反硝化作用不断提高微环境pH值,有利碳酸盐矿物在粒间孔内的成核和生长并加速颗粒胶结。

关键词：原核生物群落结构蓝细菌海滩岩南海

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Geological reservoir and resource potential(10^(13)m^(3))of gas hydrates in the south china {2.

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china Geology 2024年第3期7卷 422-444页

作者： Pi-bo Su Wei Wei yun-bao sun Yao-yao Lü Huai Cheng Wei-feng Han Wei Zhang Jin-qiang Liang National Engineering Research Center for Gas Hydrate Exploration and Development Guangzhou 511458China Sanya institute of south{2. sea Geology Guangzhou Marine Geological SurveySanya 572025China Central Laboratory of Geological sciences Research Institute of Petroleum Exploration and DevelopmentLangfang 065007China Qingdao institute of Marine Geology Qingdao 266237China Laboratory for Marine Mineral Resources Qingdao Marine Science and Technology CenterQingdao 266237China

A detailed understanding of the distribution and potential of natural gas hydrate(NGHs)resources is crucial to fostering the industrialization of those resources in the south{2. sea,where NGHs are *** this study,this study analyzed the applicability of resource evaluation methods,including the volumetric,genesis,and analogy methods,and estimated NGHs resource potential in the south{2. sea by using scientific resource evaluation methods based on the factors controlling the geological accumulation and the reservoir characteristics of ***,this study compared the evaluation results of NGHs resource evaluations in representative worldwise sea areas via rational *** results of this study are as follows:(1)The gas hydrate accumulation in the south{2. sea is characterized by multiple sources of gas supply,multi-channel migration,and extensive accumulation,which are significantly different from those of oil and gas and other unconventional resources.(2.The evaluation of gas hydrate resources in the south{2. sea is a highly targeted,stratified,and multidisciplinary evaluation of geological resources under the framework of a multi-type gas hydrate resource evaluation system and focuses on the comprehensive utilization of multi-source heterogeneous data.(3)Global NGHs resources is n×10^(15)m^(3),while the NGHs resources in the south{2. sea are estimated to be 10^(13)m^(3),which is comparable to the abundance of typical marine NGHs deposits in other parts of the *** the south{2. sea,the NGHs resources have a broad prospect and provide a substantial resource base for production tests and industrialization of NGHs.

关键词： Reservoir characteristics Natural gas hydrates Gas migration Resource potential Resource evaluation methods Hierarchical evaluation system Volumetric method south china {2. Clean energy exploration engineering

利用弹性参数反演预测潮汕坳陷目标构造的含油气性

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中国地质 2024年第5期51卷 1748-1760页

作者：钟广见强昆生杨建礼孙鸣赵静冯常茂王超易海赵忠泉阎贫刘大锰广州海洋地质调查局广东广州510760 南方海洋科学与工程广东省实验室(广州) 广东广州510760 北京珠玛阳光科技有限公司北京100083 中国科学院南海海洋研究所广东广州510301 中国地质大学(北京) 北京100083

【研究目的】潮汕坳陷构造隆起部位受砂岩储层发育程度和构造条件的双重控制,其侧翼洼陷为其提供烃源,是油气聚集最有利区。利用地震反演的弹性参数进行含油气性预测的潮汕坳陷中生界砂岩储层具有明显的低密度特征,而其他岩性的密度偏... 详细信息

【研究目的】潮汕坳陷构造隆起部位受砂岩储层发育程度和构造条件的双重控制,其侧翼洼陷为其提供烃源,是油气聚集最有利区。利用地震反演的弹性参数进行含油气性预测的潮汕坳陷中生界砂岩储层具有明显的低密度特征,而其他岩性的密度偏高。【研究方法】利用叠前地震全道集资料直接反演密度、泊松比等弹性参数,通过密度反演成果预测砂岩储层发育情况和烃类分布,实现对中生界含油气性的预测。【研究结果】泊松比对烃类比较敏感,储层含烃后具有明显的低泊松比特征。【结论】利用地震反演的弹性参数进行含油气性预测表明,烃类异常特征明显,预测构造隆起部位具有较好的含油气性。叠前弹性参数反演技术使无井区域储层预测的多解性与不确定性大大降低,提高了反演结果的准确性和可靠性,这项技术的应用对于类似研究新区的储层预测和烃类检测工作,具有很好的应用推广价值。

关键词：中生界叠前地震弹性参数反演油气勘查工程含油气性预测南海

Low-noise,low-power-consumption seafloor vector magnetometer

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Journal of oceanology and Limnology 2023年第2期41卷 804-815页

作者： Xiaochen LI Xianhu LUO ming DENG Ning QIU Zhen sun Kai CHEN china University of Geosciences Beijing 100083China guangzhou Marine Geological Survey China Geological SurveyMinistry of Natural ResourcesGuangzhou 511458Chinaa SCSIO Key Laboratory of Ocean and Marginal sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China southern Marine Science and Engineering Guangdong Laboratory(guangzhou) Guangzhou 511458China

The seafloor vector magnetometer is an effective tool for marine geomagnetic surveys and seafloor magnetotelluric(MT)***,the noise,power consumption,cost,and volume characteristics of existing seafloor vector magnetometers are insufficient for practical ***,a low-noise,low-power-consumption seafloor vector magnetometer that can be used for data acquisition of deep-ocean geomagnetic vector components is developed and presented.A seafloor vector magnetometer mainly consists of a fluxgate sensor,data acquisition module,acoustic release module,glass sphere,frame,burn-wire release,and anchor.A new low-noise data acquisition module and a fluxgate sensor greatly reduce power ***,compact size is achieved by integrating an acoustic telemetry module and replacing the acoustic release with an external burn-wire *** new design and magnetometer characteristics reduce the volume of the instrument and the cost of hardware considerably,thereby improving the integrity and deployment efficiency of the ***,it can operate for 90 days underwater at a maximum depth of 6000 *** seafloor vector magnetometer was tested in the south china sea and the Philippine sea and obtained high-quality geomagnetic *** deep-water environment facilitates magnetic field data measurements,and the magnetometer has an approximate noise level of 10 pT/rt(Hz)@1 Hz,a peak-to-peak value error of 0.2.nT,and approximate power consumption of 2.0 *** fluxgate sensor can measure the magnetic field in the lower frequency band and realize geomagnetic field measurements over prolonged periods.

关键词： seafloor vector magnetometer low noise low power consumption

Phytoplankton growth and microzooplankton grazing in the central and northern south china {2. in the spring intermonsoon {2.son of 2.17

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Acta Oceanologica Sinica 2020年第6期39卷 84-95页

作者： Dawei Chen Congcong Guo Linhui yu yuanzhen Lu Jun sun Tianjin Key Laboratory of Marine Resources and Chemistry Tianjin University of Science and TechnologyTianjin 300457China Research Centre for Indian Ocean Ecosystem Tianjin University of Science and TechnologyTianjin 300457China State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China

Phytoplankton growth rates and mortality rates were experimentally examined at 2. stations during the 2.17 spring intermonsoon(April to early May)in the northern and central south china {2.(SCS)using the dilution technique,with emphasis on a comparison between the northern and central SCS areas which had different environmental *** had been higher temperature but lower nutrients and chlorophyll a concentrations in the central SCS than those in the northern *** mean rates of phytoplankton growth(μ0)and microzooplankton grazing(m)were(0.88±0.33)d–1 and(0.55±0.2.)d–1 in the central SCS,and both higher than those in the northern SCS with the values ofμ0((0.81±0.16)d–1)and m((0.30±0.09)d–1),*** growth and microzooplankton grazing rates were significantly coupled in both *** microzooplankton grazing impact(m/μ0)on phytoplankton was also higher in the central SCS(0.63±0.12.than that in the northern SCS(0.37±0.06).The microzooplankton abundance was significantly correlated with temperature in the *** might more effectively promote the microzooplankton grazing rate than phytoplankton growth rate,which might contribute to higher m and m/μ0 in the central *** with temperature,nutrients mainly affected the growth rate of *** the nutrient enrichment treatment,the phytoplankton growth rate(μn)was higher thanμ0 in the central SCS,suggesting phytoplankton growth in the central SCS was nutrient *** ratio ofμ0/μn was significantly correlated with nutrients concentrations in the both areas,indicating the limitation of nutrients was related to the concentrations of background nutrients in the study stations.

关键词： dilution technique phytoplankton growth microzooplankton grazing south china {2. spring intermonsoon season

Parentage determination of black sea bream(Acanthopagrus schlegelii)for stock enhancement:effectiveness and loss of genetic variation

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Acta Oceanologica Sinica 2021年第6期40卷 41-49页

作者： Binbin Shan Yan liu Na Song Changping Yang Shengnan liu Tianxiang Gao Dianrong {2. Key Laboratory of south china {2. Fishery Resources Exploitation&Utilization Ministry of Agriculture Rural AffairsGuangzhou 510300China Guangdong Provincial Key Laboratory of Fishery Ecology and Environment Guangzhou 510300China Scientific Observation and Research Field Station of Pearl River Estuary Ecosystem of Guangdong Province Guangzhou 510300China south china {2. Fisheries Re{2.rch institute Chinese Academy of Fisheries SciencesGuangzhou 510300China Fishery College Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China

The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in china for a ***,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock *** order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released *** addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the *** pedigree reconstruction showed that at least 69(out of 93)broodfish had *** estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.72.-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during *** was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 12. hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in *** summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.

关键词： Acanthopagrus schlegelii stock enhancement effectiveness microsatellite maker genetic effect pedigree of hatchery populations

The influence of coupling mode of methane leakage and debris input on anaerobic oxidation of methane

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Acta Oceanologica Sinica 2021年第8期40卷 78-88页

作者： Rui Xie Daidai Wu Jie liu Guangrong Jin Tiantian sun Lihua liu Nengyou Wu Key Laboratory of Gas Hydrate Guangzhou Institute of Energy ConversionChinese Academy of SciencesGuangzhou 510640China guangzhou Marine Geological Survey China Geological SurveyMinistry of Natural ResourcesGuangzhou 510075China Institution of south china {2. Ecology and Environmental Engineering Chinese Academy of SciencesGuangzhou 510301China Evaluation and Detection Technology Laboratory of Marine Mineral Resources Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Key laboratory of Gas Hydrate Ministry of Natural ResourcesQingdao 266071China

Anaerobic oxidation of methane(AOM) is an important biogeochemical process, which has important scientific significance for global climate change and atmospheric evolution. This re{2.rch examined the δ^(34)S, terrigenous clastic indices of TiO_(2. and Al_(2.O_(3), and times for formation of the Ba front at site SH1, site SH3 and site 973-4 in the south china {2.. Three different coupling mechanisms of deposition rate and methane flux were discovered. The different coupling mechanisms had different effects on the role of AOM. At site 973-4, a high deposition rate caused a rapid vertical downward migration of the sulphate–methane transition zone(SMTZ), and the higher input resulted in mineral dissolution. At site SH3, the deposition rate and methane flux were basically in balance,so the SMTZ and paleo-SMTZ were the most stable of any site, and these were in a slow process of migration. At site SH1, the methane flux dominated the coupled mode, so the movement of the SMTZ at site SH1 was consistent with the general understanding. Understanding the factors influencing the SMTZ is important for understanding the early diagenesis process.

关键词： methane seep terrestrial detrital material anaerobic oxidation of methane gas hydrate

Talachalasins A-C,Undescribed Cytochalasans with a 16β-Methyl or 2.Oxabicyclo[3.3.1]nonan-3-one unit from the Deep-sea-Derived Fungus Talaromyces muroii *** 40439

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chinese Journal of Chemistry 2023年第8期41卷 915-923页

作者： Xuemin Ding Weixia Ye Bin tan Qiaoyun Song yuchan Chen Wei liu Lili {2. Wei tang yulei Qiao Qingbo Zhang Haibo Zhang Ying Wang Weimin Zhang Changsheng Zhang Wenjun Zhang Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Key Laboratory of Marine Materia MedicaRNAM Center for Marine Microbiology South China Sea Institute of OceanologyChinese Academy of Sciences164 West Xingang RoadGuangzhouGuangdong510301 China University of chinese academy of {2. Beijing100049 China Sanya institute of oceanology SCSIOYazhou Scientific BaySanyaHainan572000 China Center for Bioactive Natural Molecules and Innovative Drugs Research College of PharmacyJinan UniversityGuangzhouGuangdong510632 China State Key Laboratory of Applied Microbiology southern china Guangdong Institute of MicrobiologyGuangzhouGuangdong510070 China

Comprehensive Summary Talachalasins A-C(1-3),three new cytochalasans(CYTs),were isolated from deep-sea-derived fungus Talaromyces muroii *** *** structures of 1-3,including absolute configurations,were determined based on HRESIMS,NMR spectroscopic data,ECD comparison,and single crystal X-ray diffraction *** A-C(1-3)represented the first examples of cytochalasans possessing a unique 16β-methyl group,distinct from>500 reported *** C(3)was an unprecedented 2.,2.-seco-cytochalasans characterized by a 2.oxabicyclo[3.3.1]nonan-3-one *** A(1)displayed moderate cytotoxic activity against four tumor cell lines(IC_(50)3.40-10.02.M).Talachalasin B(2.showed comparable anti-RSV and HSV-1 activities(IC5012.5μM or IC502..0μM).

关键词： Cytochalasans 166-Methyl group 2.,2.-seco-Cytochalasans 2.Oxabicyclo-[3.3.1]nonan-3-one unit Cytotoxic activity

Global-Scale Diversity and Distribution Characteristics of Reef-Associated Symbiodiniaceae via the Cluster-Based Parsimony of Internal Transcribed Spacer 2.Sequences

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Journal of Ocean University of china 2021年第2期20卷 296-306页

作者： GONG Sanqiang CHAI Guangjun sun Wei ZHANG Fengli yu Kefu LI Zhiyong Marine Biotechnology Laboratory State Key Laboratory of Microbial Metabolism and School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Tropical Marine Bioresources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of OceanologyGuangzhou 510301China southern Marine Science and Engineering Guangdong Laboratory(guangzhou) Guangzhou 511458China Coral Reef Research Center of china Guangxi UniversityNanning 530004China

Photosynthetic species belonging to Symbiodiniaceae play important ecological roles in coral reef ecosystems. Nine phylogenetically supported Symbiodiniaceae clades(each containing multiple genetically distinct molecular types) have been detected in reef-associated animals and in seawater or sediments near coral reef areas. Although major advances in research on Symbiodiniaceae diversity have been made, information on the global-scale diversity and distribution characteristics of Symbiodiniaceae clades/types is still limited or must be expanded because most studies on Symbiodiniaceae have mainly focused on a limited number of hosts associated with Symbiodiniaceae or coral reef areas. Here, the diversity and distribution characteristics of Symbiodiniaceae data derived from reef-associated animals and seawater or sediments near coral reef areas were analyzed using 3899 qualified Symbiodiniaceae internal transcribed spacer 2.ITS2. sequences retrieved from the GenBank database in May 2.17. These sequences were annotated as Symbiodiniaceae ITS2.types via sequence alignment against database of Symbiodiniaceae ITS2.types. Results showed that photosynthetic Symbiodiniaceae ITS2.sequences could be annotated as 119 Symbiodiniaceae ITS2.types within nine clades(A–I), and Symbiodiniaceae ITS2.types mainly inhabited coral reefs between 30?N and 30?S. Symbiodiniaceae ITS2.types could be associated with multiple host taxa. Interestingly, these results implied that the distribution of Symbiodiniaceae ITS2.types was distinct in the Atlantic Ocean, the Red sea, the Mediterranean sea, and the Indo-Pacific Ocean. Although future field research should be performed to verify our results, this study provided a broad perspective of the diversity of Symbiodiniaceae clades/types on a global scale.

关键词： Symbiodiniaceae diversity reef-dwelling animals coral reefs global scale