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An Arabidopsis ctpA homologue is involved in the repair of photosystem II under high light

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Chinese Science Bulletin 2008年第7期53卷 1021-1026页

作者： YIN ShuMing sun xuwu ZHANG LiXin School of Life Sciences Lanzhou University Lanzhou 730000 China Photosynthesis Research Center Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

A T-DNA insertion mutant AtctpA1 was identified to study the physiological roles of a carboxyl-terminal processing protease (CtpA) homologue in Arabidopsis. Under normal growth conditions, disruption of AtctpA1 did not result in any apparent alterations in growth rate and thylakoid membrane protein components. However the mutant plants exhibited increased sensitivity to high irradiance. Degradation of PSII reaction center protein D1 was accelerated in the mutant during photoinhibition. These results demostrated that AtctpA1 was required for efficient repair of PSII in Arabidopsis under high irradiance.

关键词： ctpA 同源染色体光合作用强光

Involvement of DEG5 and DEG8 proteases in the turnover of the photosystem II reaction center D1 protein under heat stress in Arabidopsis thaliana

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Chinese Science Bulletin 2007年第13期52卷 1742-1745页

作者： sun xuwu WANG LiYuan ZHANG LiXin Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou 730000 China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Photosynthesis Research Center Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Deg5,deg8 and the double mutant,deg5deg8 of Arabidopsis thaliana were used to study the physiological role of the DEG proteases in the repair cycle of photosystem II (PSII) under heat stress. PSII activity in deg mutants showed increased sensitivity to heat stress, and the extent of this effect was greater in the double mutant, deg5deg8, than in the single mutants, deg5 and deg8. Degradation of the D1 protein was slower in the mutants than in the WT plants. Furthermore, the levels of other PSII reaction center proteins tested remained relatively stable in the mutant and WT plants following high-temperature treatment. Thus, our results indicate that DEG5 and DEG8 may have synergistic function in degradation of D1 protein under heat stress.

关键词： D1蛋白蛋白酶阿布属光合作用

Deg1 is involved in the degradation of the PsbO oxygen-evolving protein of photosystem Ⅱ in Arabidopsis

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Chinese Science Bulletin 2010年第27期55卷 3145-3148页

作者： LI Jin sun xuwu ZHANG LiXin School of Life Sciences Lanzhou University Lanzhou 730000 China Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Deg1,a thylakoid lumen-localized protease,retains both chaperone and protease *** in vivo function of Deg1 has been shown to be involved not only in PSII assembly but also in the degradation of PSII reaction center protein *** we used the transgenic plants with reduced Deg1 to examine whether the lumen-localized proteins are also the substrates of Deg1 in *** results showed that the transgenic plants accumulated degradation products of the PsbO protein while the levels of full-length PsbO were not *** PsbO degradation products could be efficiently degraded by the recombinant *** results suggest that Deg1 is involved in the degradation of the PsbO degradation fragments,but not in the initial cleavage event itself.

关键词：降解产物蛋白酶光系统II 拟南芥放氧转基因植物转基因植株类囊体腔

Increased sensitivity to drought stress in atlon4 Arabidopsis mutant

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Chinese Science Bulletin 2010年第32期55卷 3668-3672页

作者： LI XiaoYi MU Ying sun xuwu ZHANG LiXin School of Life Sciences Lanzhou University Lanzhou 730000 China Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The ATP-dependent serine protease Lon belongs to the AAA+ superfamily,which is widely distributed in bacteria,archaea,and eukaryotic cells. Lon participates in the regulation of numerous physiological processes and gene expression via degrading various unstable and non-native regulators. In this study,we showed that the Arabidopsis atlon4 mutant is more sensitive to drought stress than wild-type plants. Compared with wild-type plants,atlon4 mutant plants showed increased water loss,decreased water use efficiency,and impaired stomatal closure in drought stress conditions.

关键词：干旱胁迫突变体敏感性拟南芥丝氨酸蛋白酶监管机构水分流失真核细胞

Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem Ⅱ under high-light condition

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Journal of Integrative Plant Biology 2014年第12期56卷 1136-1150页

作者： Haomeng Yang Libing Liao Tingting Bo Lei Zhao xuwu sun Xuefeng Lu Birgitta Norling Fang Huang Key Laboratory of Photobiology Institute of Botany the Chinese Academy of Sciences University of the Chinese Academy of Sciences Qingdao Institute of Bioenergy and Bioprocess Technology the Chinese Academy of Sciences School of Biological Sciences Nanyang Technological University

Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ（PSⅡ） in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is *** have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis *** 6803. Here, we report that Slr0151 is involved in PSⅡ repair of the organism. We generated a mutant strain（Dslr0151）lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSⅡ activity in Synechocystis. Moreover,the mutant exhibited increased photoinhibition due to impairment of PSⅡ repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSⅡ repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSⅡ in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together,these results indicate that Slr0151 plays an important role in regulating PSⅡ repair in the organism under high-light stress condition.

关键词： Cyanobacteria membrane protein photosystem Ⅱ repair Slr0151 Synechocystis

Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum

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Science Bulletin 2022年第3期67卷 315-327页

作者： Chen Dong Ge Qu Jinggong Guo Fang Wei Shuwen Gao Zhoutong sun Lifeng Jin xuwu sun Jean-David Rochaix Yuchen Miao Ran Wang Zhengzhou Tobacco Research Institute Zhengzhou 450001China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China College of Biological Engineering Henan University of TechnologyZhengzhou 450001China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China Departments of Molecular Biology and Plant Biology University of GenevaGeneva 1211Switzerland

Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and *** this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nicotiana *** the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of *** of this enzyme in the ntggpps1 mutant leads to decreased carotenoid *** the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in combination with sequence *** activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput colorimetric assay.d-NtGGPPS1 exhibited significantly higher conversion of IPP and each co-substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis compared with wild-type ***,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to *** findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield *** results open the door for the structure-guided rational design of elite genes in higher plants。

关键词： Rational design Carotenoid Methylerythritol phosphate pathway(MEP) Geranylgeranyl diphosphate synthase(GGPPS) Nicotiana tabacum

AtCNC mediates chloroplast to nuclear signalling

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AtCNC mediates chloroplast to nuclear signalling

Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing

2010全国植物生物学研讨会

作者： xuwu sun Peiqiang Feng Xiumei Xu Lixin Zhang Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences

Plastids play central roles in photosynthesis and other metabolic pathways and thus are of fundamental importance for life on *** development and gene expression are mainly regulated by nuclear-encoded proteins,however,retrograde signal from chloroplast to nuclear modulates the expression of nuclear genes encoding

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Molecular Plant 2020年第8期13卷 1178-1193页

作者： Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao xuwu sun State Key Laboratory of Crop Stress Adaptation and Improvement State Key Laboratory of Cotton BiologyKey Laboratory of Plant Stress BiologySchool of Life SciencesHenan University85 Minglun StreetKaifeng 475001China College of Life Sciences Shanghai Normal UniversityGuilin Road 100Shanghai200234China Departments of Molecular Biology and Plant Biology University of GenevaGeneva1211Switzerland

The regulation of stomatal lineage cell development has been extensively ***,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been *** this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis *** identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker *** analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother *** of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these ***,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.

关键词： molecular profiling stomatal development single-cell RNA-seq

Genetic dissection of chloroplast development

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Genetic dissection of chloroplast development