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利用人工锌指文库选育高乙醇耐受性工业酵母菌株

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生物工程学报 2013年第5期29卷 612-619页

作者：马翠赵心清李倩张明明 jin soo kim 白凤武大连理工大学生命科学与技术学院辽宁大连116024 ToolGen IncSeoul153-023South Korea

选育高乙醇耐性的酿酒酵母菌株对提高燃料乙醇的发酵效率具有重要意义。锌指蛋白广泛存在于多种生物中,对基因的转录和翻译起重要的调节作用。利用人工设计的锌指蛋白可定向设计锌指序列及其排列顺序,实现对细胞内多个基因的全局调控。... 详细信息

选育高乙醇耐性的酿酒酵母菌株对提高燃料乙醇的发酵效率具有重要意义。锌指蛋白广泛存在于多种生物中,对基因的转录和翻译起重要的调节作用。利用人工设计的锌指蛋白可定向设计锌指序列及其排列顺序,实现对细胞内多个基因的全局调控。由于与环境胁迫反应相关的基因很多,因此可利用人工锌指蛋白技术获得耐受性提高的微生物重组菌。文中将人工锌指文库转入到酿酒酵母模式菌株S288c,选育了具有高乙醇耐受性的重组菌株M01,并分离了与乙醇耐受性提高相关的人工锌指蛋白表达载体pRS316ZFP-M01,转入工业酿酒酵母Sc4126,在含有不同浓度乙醇的平板上,工业酵母Sc4126的重组菌株表现出显著的耐受性提高。在高糖培养基(250 g/L)条件下进行乙醇发酵,发现重组菌的乙醇发酵效率明显快于野生型,发酵时间提前24 h,且发酵终点乙醇浓度提高6.3%。结果表明人工锌指文库能够提高酵母的乙醇耐受性,为构建发酵性能优良的酵母菌种奠定了基础。

关键词：酿酒酵母人工锌指蛋白乙醇耐受性乙醇发酵

A Screening Echocardiography-Based Comparison between the Prehypertensive and the Normal

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A Screening Echocardiography-Based Comparison between the Pr...

Exercise training mode effects on myokine expression in healthy adults:A systematic review with meta-analysis

2010中华医学会影像技术分会第十八次全国学术大会

作者： soo-jin kim Hye-jung koo Hyun-bok Lee Nam-soo Cho Samsung Seoul Hospital Radiology Department

Objective:There have been many studies on the correlation between left ventricular hypertrophy(LVH) and left ventricular diastolic function in hypertensive ***,there have been few studies on‘Prehypertension’,i.e.,a ...

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Journal of Sport and Health Science 2024年第6期13卷 764-779页

作者： Francesco Bettariga Dennis R.Taaffe Daniel A.Galvao Pedro Lopez Chris Bishop Anna Maria Markarian Valentina Natalucci jin-soo kim Robert U.Newton Exercise Medicine Research Institute Edith Cowan UniversityJoondalupWA 6027Australia School of Medical and Health Sciences Edith Cowan UniversityJoondalupWA 6027Australia Pleural Medicine Unit Institute for Respiratory HealthPerthWA 6009Australia Medical School Faculty of Health&Medical SciencesUniversity of Western AustraliaPerthWA 6009Australia Grupo de Pesquisa em Exercicio para Populacoes Clinicas(GPCLIN) Universidade de Caxias do SulCaxias do SulRio Grande do Sul 95070-560Brazil London Sport Institute School of Science and TechnologyMiddlesex UniversityLondonNW44BTUK Department of Pathophysiology and Transplantation University of MilanMilan 20133Italy School of Human Movement and Nutrition Sciences University of QueenslandSt.LuciaQLD 4072Australia

Background:The benefits of exercise are well known;however,many of the underlying molecular mechanisms are not fully *** muscle secretes myokines,which mediate muscleorgan *** regulate satellite-cell proliferation and migration,inflammatory cascade,insulin secretion,angiogenesis,fatty oxidation,and cancer *** date,the effects of different exercise modes(namely,aerobic and resistance exercise)on myokine response remain to be *** is crucial considering the clinical implementation of exercise to enhance general health and wellbeing and as a medical ***:A systematic search was undertaken in PubMed,MEDLINE,CINAHL,Embase,SPORTDiscus,andWeb of Science in April *** studies examining the effects of a single bout of exercise on interleukin15(IL-15),irisin,secreted protein acidic and rich in cysteine(SPARC),oncostatinM(OSM),and decorin were included.A random-effects meta-analysis was also undertaken to quantify the magnitude of ***:Sixty-two studies were included(n=1193).Overall,exercise appeared to induce small to large increases in myokine expression,with effects observed immediately after to 60 min post-exercise,although these were mostly not statistically *** aerobic and resistance exercise resulted in changes in myokine levels,without any significant difference between training modes,and with the magnitude of change differing across *** levels returned to baseline levels within 180 min to 24 h ***,owing to potential sources of heterogeneity,most changes were not statistically significant,indicating that precise conclusions cannot be ***:Knowledge is limited but expanding with respect to the impact of overall and specific effects of exercise on myokine expression at different time points in the systemic *** research is required to investigate the effects of different exercise modes at multiple time points on myok

关键词： Myokine Resistance exercise Aerobic exercise Cytokine Systemic circulation

Apolipoprotein A-I binding protein-mediated neuroprotection in glaucomatous neuroinflammation and neurodegeneration

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Neural Regeneration Research 2025年第5期20卷 1414-1415页

作者： Sinwoo Hwang Seunghwan Choi soo-Ho Choi Keun-Young kim Yury I.Miller Won-Kyu Ju Hamilton Glaucoma Center and Shiley Eye Institute The Viterbi Family Department of OphthalmologyUniversity of California San DiegoLa JollaCAUSA Department of Medicine University of California San DiegoLa JollaCAUSA National Center for Microscopy and Imaging Research Department of NeurosciencesUniversity of California San DiegoLa JollaCAUSA

Glaucoma is a multifactorial eye disorder that can cause vision loss and irreversible blindness,affecting individ uals aged 40 to 80 yea rs *** to the aging population,it is expected that the number of people affected by glaucoma will surpass 111 million by 2040 as the disease becomes more *** primarily contributes to optic nerve axon loss and the progressive degeneration of retinal ganglion cells (RGCs),subsequently leading to vision impairment.

关键词： irreversible pass primarily

羟考酮/纳洛酮控释片与羟考酮控释片治疗韩国癌性疼痛患者的疗效及安全性的随机对照研究

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癌症 2018年第5期37卷 179-187页

作者： Kyung-Hee Lee Tae Won kim Jung-Hun Kang jin-soo kim jin-Seok Ahn Sun-Young kim Hwan-Jung Yun Young-Jun Eum Sung Ae Koh Min Kyoung kim Yong Sang Hong Jeong Eun kim Gyeong-Won Lee Department of Hematology-Oncology Yeungnam University Medical Center Department of Oncology Asan Medical Center University of Ulsan Department of Oncology Asan Medical Center University of Ulsan College of Medicine Department of Hematology-Oncology Gyeongsang National University Hospital Department of Internal Medicine SMG-SNU Boramae Medical Center Division of Hematology-Oncology Department of Medicine Samsung Medical Center Department of Hematology-Oncology National Cancer Center Department of Hematology-Oncology Chungnam National University Hospital Medical Affairs Mundipharma Korea Ltd

背景与目的羟考酮/纳洛酮控释片(controlled?release oxycodone/naloxone,OXN?CR)通过羟考酮发挥阿片类药物诱导的镇痛效果,同时纳洛酮可降低阿片类药物引起的便秘的发生率。本研究旨在评价与羟考酮控释片(controlled?release oxycodone... 详细信息

背景与目的羟考酮/纳洛酮控释片(controlled?release oxycodone/naloxone,OXN?CR)通过羟考酮发挥阿片类药物诱导的镇痛效果,同时纳洛酮可降低阿片类药物引起的便秘的发生率。本研究旨在评价与羟考酮控释片(controlled?release oxycodone,OX?CR)相比,OXN?CR在控制韩国患者癌性疼痛中的非劣效性。方法本项随机、开放标签、平行分组、IV期研究中,我们从韩国的7个肿瘤学/血液学中心招募了年龄20岁及以上、具有中重度癌性疼痛的患者[疼痛数字评价量表(numeric rating scale,NRS)疼痛评分≥4分]。意向治疗人群(intention天20 mg/10 mg,每天最大?to用?treat,ITT)随机(1:1)分为OXN?CR和OX?CR组。OXN?CR组:起始用药量为每药量80 mg/40 mg,用药4周。OX?CR组:起始用药量为20 mg/d,最大用药量为80 mg/d,用药4周。本研究的主要疗效终点为NRS疼痛评分从基线到第4周的变化,其非劣效性界值为-1.5。次要终点包括应急镇痛药摄入量、患者报告的排便习惯改变、缓泻药摄入量、生活质量(quality of life,QoL)及安全性评价。结果 128例患者组成的ITT人群中,7例因缺失主要疗效数据、4例因不符合资格标准被排除疗效分析。在第4周时,OXN?CR组(n=58)和OX?CR组(n=59)患者NRS疼痛评分的平均变化无显著性差异(-1.586 vs.-1.559,P=0.948)。组间差异的单侧95%置信区间(-0.776-0.830)的下限超过非劣效性边界(P <0.001)。OXN?CR和OX?CR两组患者在应急镇痛药摄入量、排便习惯改变、缓泻药摄入量、QoL和安全性评价方面均无显著差异。结论就治疗4周后减轻患者疼痛的效果而言,OXN?CR的疗效非劣于OX?CR,两者具有相似的安全性。对于OXN?CR在长期疼痛控制和缓解便秘方面的疗效,则需在更多韩国癌性疼痛患者人群中开展进一步的研究。

关键词：便秘纳洛酮羟考酮生活质量安全性

Biogeochemical cyclic activity of bacterial arsB in arsenic-contaminated mines

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Journal of Environmental Sciences 2008年第11期20卷 1348-1355页

作者： CHANG jin-soo kim Kyoung-Woong Department of Environment Science and Engineering Gwangju Institute of Science and Technology (GIST)261 Cheomdan-gwagiroBuk-guGwangjuRepublic of Korea.

Biogeochemical cyclic activity of the ars (arsenic resistance system) operon is arsB influx/effux encoded by the ecological of Pseudomonas *** suggests that studying arsenite-oxidizing bacteria may lead to a better understanding of molecular geomicrobiology,which can be applied to the bioremediation of arsenic-contaminated *** is the first report in which multiple arsB-binding mechanisms have been used on indigenous *** ArsB (strains OS-5; ABB83931; OS-19; ABB04282 and RW-28; ABB88574...

关键词： arsenic resistance bacteria arsB influx/efflux pump arsenic resistance system (ars) biogeochemical

Ultimate strength of single shear bolted connections with cold-formed ferritic stainless steel

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第2期14卷 120-136页

作者： jin-seong LIM Tae-soo kim Seung-hun kim Department of Architectural Engineering Hanbat National UniversityDaejeon 305-719Korea

This paper is focused on the structural behavior of the single shear bolted connections with thin-walled ferritic stainless *** purpose of this study is to investigate the ultimate behaviors,such as ultimate strength and fracture mode of the single shear bolted connections of thin-walled ferritic stainless steel(low cost steel) rather than austenitic stainless steel(high cost steel).Bolt arrangement and end distance parallel to the direction of applied load are considered as main variables of the test specimens for bolted *** have a constant dimension of edge distance perpendicular to the loading direction,bolt diameter,pitch,and gauge.A monotonic tensile test for specimens has been carried out and some bolted connections with long end distance showed curling(out of plane deformation) occurrence which led to strength *** ultimate behaviors such as fracture mode,ultimate strength are compared with those predicted by current design ***,conditions of curling occurrence and the strength reduction due to curling are investigated and modified strength equations are suggested considering the curling effect.

关键词： Cold-formed ferritic stainless steel Bolted connection Bolt arrangement End distance Curling Strength reduction

Amine functionalization on thermal and mechanical behaviors of graphite nanofibers-loaded epoxy composites

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Journal of Materials Science & Technology 2023年第20期151卷 80-88页

作者： Seong-Hwang kim Sang-jin Park Seul-Yi Lee soo-jin Park Department of Chemistry Inha University100 InharoIncheon 22212the Republic of Korea

With the growing demand for faster and more powerful computing,effective heat dissipation is essential to ensure the longevity,reliability,and high performance of electronic *** the field of modern electronic packaging materials,there is a great need for graphitic material-loaded polymeric composites(GPCs)with excellent thermal ***,the enhancement efficiency of GPCs is hindered by the agglomeration of fillers and the interfacial thermal resistance caused by the lack of continuous thermally conductive pathways between the filler and *** the interfaces between filler and matrix is of great importance in optimizing the performances of ***,we fabricated graphite nanofibers(GNF)-loaded nanocomposites using acid-functionalized GNF(AGNF)and acidtetraethylenepentamine-functionalized(TGNF)as a filler and epoxy resin as a matrix with different GNF loading contents to explore the interfacial properties of the *** optimal GNF loading for AGNF was 0.5 wt.%,while the TGNF showed 0.75 wt.%.The highest thermal conductivity(0.51 W m^(−1) K^(−1))and fracture toughness(25.8 MPa m^(1/2))values were found in the TGNF-loaded nanocomposites with a fraction of 0.75 wt.%,representing enhancements of∼145%and∼400%,respectively,compared to those of neat *** experimental data presented herein demonstrate that the interfacial properties play a significant role in enhancing the thermal and mechanical performances of the *** present approach is expected to serve as a valuable tool in the design of conductive polymeric nanocomposites for further practical applications,such as thermal interface materials and packaging of high-power electric devices.

关键词： Graphite nanofiber Nanocomposites Interfacial interaction Thermal conductivity Fracture resistance

User Interface-Based Repeated Sequence Detection Method for Authentication

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Intelligent Automation & Soft Computing 2023年第3期35卷 2573-2588页

作者： Shin jin Kang soo Kyun kim School of Games Hongik UniversitySejong30016Korea Department of Computer Engineering Jeju National UniversityJeju63243Korea

In this paper,we propose an authentication method that use mouse and keystroke dynamics to enhance online privacy and *** proposed method identiﬁes personalized repeated user interface(UI)sequences by analyzing mouse and keyboard *** this end,an Apriori algorithm based on the keystroke-level model(KLM)of the human–computer interface domain was *** proposed system can detect repeated UI sequences based on KLM for authentication in the *** effectiveness of the proposed method is veriﬁed through access test-ing using commercial applications that require intensive UI *** results show using our cognitive mouse-and-keystroke dynamics system can com-plement authentication at the application level.

关键词： Authentication keystroke-level model(KLM) keystroke mouse dynamics