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Characterization of Nanocrystallizatin Surface Layer of 0.4C-1Cr Low Alloy Steel Prepared by Ultrasonic Particulate Peening

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材料热处理学报 2004年第05B期25卷 1257-1259页

作者： ZHANGJun-bao LIUYu-liang ZHAOXin-qi WUJie songhong-wei XIONGTian-ying BaosteelTechnologyCenter BaoshanIron＆SteelCO.LTD.ShanghaiChina InstituteofMetalResearch ChineseAcademyofSciencesShenyangChina

A nanostructured surface layer was fabricated in a quenched and tempered 0.4C-lCr low alloy steel by ultrasonic particulate peening technique. The microstructure of the nanocrystalline surface layer was characterized by means of TEM and Mossbauer spectroscopy. Experimental results reveal that both cementite and ferrite nanocrystals with an average size of 5 nm were formed in the surface layer of the steel, phase transformation of austenite and dissolution of cementite maybe occur in the process of ultrasonic paniculate peening.

关键词：显微结构超声波喷丸处理表面毫微结晶 0.4C-1Cr低合金钢

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Identification and Characterization of Plant Cell Death-Inducing effector proteins from Rhizoctonia solani

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Identification and Characterization of Plant Cell Death-Indu...

中国植物病理学会2017年学术年会

作者： Li Shuai Fang Rui Jia Shentong wei songhong College of Plant Protection Shenyang Agricultural University

Rice sheath blight,caused by Rhizoctonia solani,is one of the most important fungus diseases of ***,little has been known about molecular mechanisms underlying the function（s） of effectors in *** virulence pathogenicity so *** and characterization of novel effector proteins are crucial for understanding pathogen virulence and host-plant defense *** previous studies,genome of *** AG1 IA reveals many potential genes coding *** analysis indicated that some effectors contain structure which may contribute to ***,we performed functional studies on putative effectors in *** and demonstrated that 3 of 40 putative effectors caused necrosis or necrosis-like phenotypes in Nicotiana *** them,1 protein signal peptide play important role in triggering cell death in *** results will provide useful information for insight into pathogenic factors for ***.

关键词： Rhizoctonia solani Effector Plant Immunity Pathogenicity

Identification of physiological races and avirulence genes of Pyricularia oryzae in Liaoning province in 2017

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Identification of physiological races and avirulence genes o...

中国植物病理学会2019年学术年会

作者： Liu wei wei songhong Zhu Lijun Wang Haining Zhang Zhaoru Li Xinyang College of Plant Protection Shenyang Agricultural University

In order to understand the dynamics of physiological races and the composition of avirulence genes of Pyricularia oryzae and provide a theoretical basis for rational distribution of rice varieties and breeding of blast-resistant cultivars in Liaoning province,7 identified rice varieties were used to identify the physiological race of 75 isolates of Pyricularia oryzae collected in 2017 and the primers were designed to amplify the target sequences based on the sequences of 10 Avr-genes which had been *** results showed that the tested isolates were divided into 6 groups of 32 physiological races,ZA and ZB were the predominant groups,accounting for 42.67% and 28.00%,respectively,ZC,ZD,ZE and ZF were 9.33%,5.33%,4.00% and 10.67%.ZG was not *** the test isolates carried the avirulence genes Avr-Pi9 and *** carried the avirulence genes Avr-Pita,Avr-Piz-t,Avr-Pik,PWL2 and Avr-Pib,carrying frequencies of 92%,94.67%,93.33%,93.33% and49.33%.The avirulence genes Avr-Pii,Avr-C039 and Avr-Pia were not *** summary,the composition and structure of the physiological races and avirulence genes of Pyricularia oryzae are complex in Liaoning *** the rice variety layout,the rice cultivars carrying resistant genes Pita,Pi9,Pizt and Pik should be generalized in Liaoning province.

关键词： Liaoning Province Pyricularia oryzae physiological races avirulence gene

Biological characteristics of Ustilaginoidea virens from different regions in Liaoning province

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Biological characteristics of Ustilaginoidea virens from dif...

中国植物病理学会2019年学术年会

作者： Li Xinyang wei songhong Li Shuai Wang Haining Zhu Lijun Zhang Zhaoru Liu wei College of Plant Protection Shenyang Agricultural University

In order to reveal the relationship between the mycelial growth of Ustilaginoidea virens and the environmental conditions from different regions in Liaoning Province,and understand its infection *** results would provide a scientific basis for forecasting,control and resistance breeding of the rice false *** pathogens were isolated and purified by Streptomycin *** characteristics of Ustilaginoidea virens were determined by using the crossing *** results showed that the mycelia of the pathogen grew better on the medium OA、XBZ、PSA and *** could grow at temperature of 10℃to 30℃,best at 25-30℃.They grew well at pH varied from 3 to 12,best at *** could utilize many substances as carbon and nitrogen *** carbon sources tested,starch and sucrose were found to be the best ones for the growth of Ustilaginoidea *** extract and peptone were the most suitable nitrogen *** was good for mycelial *** lethal temperature for the mycelium was 55℃.Based on the studies about biological characteristics of Ustilaginoidea virens,apart from chemical and biological controls,the growth of Ustilaginoidea virens can be inhibited by changing cultivation environment,reasonable fertilization,irrigation,planting density and rotation,so as to reduce the disease damage.

关键词： Liaoning Province Rice False Smut Ustilaginoidea virens Biological Characteristics

A Secreted Protein from Rhizoctonia solani Is Essential For The Necrotrophic Interactions With Rice

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A Secreted Protein from Rhizoctonia solani Is Essential For ...

中国植物病理学会2021年学术年会

作者： LI Shubin HAI Yingfan XIANG Zongjing YE Long WANG Yan wei songhong LI Shuai College of Plant Protection Shenyang Agricultural University

The influence of effectors which is secreted by necrotrophic pathogens during infection has growing attention ***,little is known about the virulence and mechanisms of these *** solani,which cause rice sheath blight,is one of the most significant necrotrophic *** studies have been shown that genome of *** AG1 IA reveals many potential genes coding *** analysis indicated that some effectors contain structure which may contribute to ***,we performed functional studies on putative effectors in *** and demonstrated that AGLIP1 could induce cell death at 4 days after infiltration by ***,AGLIP1 inhibited BAX-induced and INF1-induced HR response in Nicotiana *** research has revealed AGLIP1 was up-regulated during *** ***1 could interact with OsClpP6 which is a caseinolytic protease in ***1 decreased the accumulation of OsClpP6 in Nicotiaua *** results will provide new outlooks in understanding the molecular mechanisms of *** pathogenesis and rice resistance.

关键词： Rhizoctonia solani Effector Plant Immunity Pathogenicity

Mechanism of immunization of rice seedlings

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Rice Science 1998年第1期 4-6页

作者： YU Yunzhen wei songhong LIU Zhiheng MAO Guojie YANG diashu Plant Protection Dept,Shenyang Agri Univ,Shenyang 110161,China

In saline paddy soil in the north of China, thetechnique of immunization of rice nursery(IRN) was applied in 1990’s and successfullymet the production needs. It had more advan-tages than paddy soil land nursery (PSLN).

关键词： IRN PAL In SOD Mechanism of immunization of rice seedlings

Mechanism of immunization of rice seedlings

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Chinese Rice Research Newsletter 1998年第1期6卷 4-6页

作者： YU Yunzhen, wei songhong, LIU Zhiheng, MAO Guojie, and YANG Jiashu, Plant Protection Dept, Shenyang Agri Univ, Shenyang 110161, China

In saline paddy soil in the north of China,thetechnique of immunization of rice nursery(IRN)was applied in 1990’s and successfullymet the production *** had more advan-tages than paddy soil land nursery(PSLN).

关键词： SOD IRN

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Quantitative detection of Fusarium graminearum and its chemotypes by TaqMan PCR

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Quantitative detection of Fusarium graminearum and its chemo...

中国菌物学会第六届会员代表大会（2014年学术年会）暨贵州省食用菌产业发展高峰论坛

作者： songhong wei Theo van der Lee Richard van Hoof Marga van Gent-Pelzer Els verstappen Cees Waalwijk Zhiheng Liu College of Plant Protection Shenyang Agricultural University Plant Research International Wageningen University and Research Centre

Fusarium graminearum species complex(Fg complex)are common cereal pathogens that cause destructive and economically important diseases worldwide,such as Fusarium head blight,maize ear *** causing severe production and quality losses,the Fg complex can produce trichothecene mycotoxins that pose a significant risk to food safety and animal *** studies indicated that Fg complex consisted of at least 11 phylogenetically distinct species and produced one of three strain-specific chemotypes of B-trichothecene:nivalenol and acetylated derivatives(NIV chemotype),deoxynivalenol and primarily 3-acetyldeoxynivalenol(3ADON chemotype),and deoxynivalenol and primarily 15-acetyldeoxynivalenol(15ADON chemotype).To quantify the *** and its chemotypes,the TaqMan PCR was *** on ammonia ligase 2gene and TRI3 and TRI12 genes,TaqMan primers and probes were designed that showed high specificity for *** and its chemotypes,3ADON,15 ADON and NIV within NRRL and Bayer reference isolates and the chemotypes were confirmed with Luminex-Multilocus genotyping *** analysis of aberrant TaqMan profiles,also observed by Biotrove OpenArray real-time PCR assay,showed one to three mismatches existed in primers and probe sequences.

关键词： Fusarium graminearum 3ADON 15ADON NIV TaqMan PCR

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具有除草活性的小麦根腐病菌发酵条件优化

植物保护 2012年第4期38卷 77-82页

作者：魏松红张丽莹王黎张景远李斐沈阳农业大学植物保护学院沈阳110866

通过单因子试验和正交试验对小麦根腐病菌菌株进行了发酵条件优化,以提高其活性物质的产量。结果表明:最适合小麦根腐病菌产毒的培养基是察氏培养基;该培养基的最佳碳源为葡萄糖,氮源为氯化铵,无机盐为氯化钾、磷酸氢二钾、硫酸镁和硫... 详细信息

通过单因子试验和正交试验对小麦根腐病菌菌株进行了发酵条件优化,以提高其活性物质的产量。结果表明:最适合小麦根腐病菌产毒的培养基是察氏培养基;该培养基的最佳碳源为葡萄糖,氮源为氯化铵,无机盐为氯化钾、磷酸氢二钾、硫酸镁和硫酸亚铁;发酵培养基各组分的最佳配比为:葡萄糖2.0%,氯化铵0.25%,氯化钾0.05%,磷酸氢二钾0.025%,硫酸镁0.025%,硫酸亚铁0.000 5%;有利于提高菌株活性物质产量的培养条件为初始pH7.0,转速为130r/min,温度为28℃,培养时间为5d,装液量为120mL/250mL,接种量为6%。小麦根腐病菌菌株发酵产物具有良好的热稳定性和光稳定性,在弱酸性及中性条件下较稳定。发酵条件优化后提高了小麦根腐病菌的除草活性。

关键词：除草活性小麦根腐病菌发酵条件优化

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