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一种曳引驱动电梯对重越程距离的标识方法

中国电梯 2024年第5期35卷 72-73,76页

作者：宋春鹏王刚葛艳日照市特种设备检验科学研究院山东日照276800

有些安装维保人员对曳引驱动电梯的对重越程距离的计算、标识存在疑惑,导致对重越程距离标识不规范。结合相关国家标准和安全技术规范的要求,介绍一种曳引驱动电梯对重越程距离的标识方法,包括如何测量数据,如何计算最大值和最小值,进... 详细信息

有些安装维保人员对曳引驱动电梯的对重越程距离的计算、标识存在疑惑,导致对重越程距离标识不规范。结合相关国家标准和安全技术规范的要求,介绍一种曳引驱动电梯对重越程距离的标识方法,包括如何测量数据,如何计算最大值和最小值,进而规范、简捷地标识出对重越程距离。规范地标识对重越程距离,有助于电梯维保人员和检验检测人员在工作中及时发现对重越程距离的变化,及时做出调整,防范电梯风险。

关键词：曳引驱动电梯越程距离测量方法计算方法标识线

BAK1 plays contrasting roles in regulating abscisic acid-induced stomatal closure and abscisic acid-inhibited primary root growth in Arabidopsis

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Journal of Integrative Plant Biology 2022年第6期64卷 1264-1280页

作者： Jinping Deng Lingyao Kong Yinhua Zhu Dan Pei Xuexue Chen Yu Wang Junsheng Qi chunpeng song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Life Sciences Qingdao UniversityQingdao 266071China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001HenanChina Institute of Life Science and Green Development School of Life SciencesHebei UniversityBaoding 071002China

The mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid(ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1(BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1(OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1-1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1-2 abi2-2 hab1-1 loss-of-function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2 Cs,and Sn RK2 s, during seed germination and primary root growth. Although the upstream brassinosteroid(BR) signaling components BAK1 and BR INSENSITIVE 1(BRI1) positively regulate ABAinduced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID-SIGNALING KINASEs(BSKs)and BRASSINOSTEROID-INSENSITIVE 2(BIN2), did not affect ABA-mediated stomatal movement. Thus,our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA-induced stomatal closure, thus optimizing the plant growth under drought stress.

关键词： abscisic acid BAK1 primary root growth stomatai closure

RAF22,ABI1 and OST1 form a dynamic interactive network that optimizes plant growth and responses to drought stress in Arabidopsis

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Molecular Plant 2022年第7期15卷 1192-1210页

作者： Zhihui Sun Zhenkai Feng Yanglin Ding Yuanpeng Qi Shan Jiang Zhen Li Yu Wang Junsheng Qi chunpeng song Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Plants adapt to their ever-changing environment via positive and negative signals induced by environmental *** stress,for instance,induces accumulation of the plant hormone abscisic acid(ABA),triggering ABA signal ***,the molecular mechanisms for switching between plant growth promotion and stress response remain poorly *** we report that RAF(rapidly accelerated fibrosarcoma)-LIKE MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 22(RAF22)in Arabidopsis tha/iana physically interacts with ABA INSENSITIVE 1(ABl1)and phosphorylates ABl1 at Ser416 residue to enhance its phosphatase ***,ABl1 can also enhance the activity of RAF22 through dephosphorylation,reciprocally inhibiting ABA signaling and promoting the maintenance of plant growth under normal *** drought stress,however,the ASA-activated OPEN STOMATA1(OST1)phosphorylates the Ser81 residue of RAF22 and inhibits its kinase activity,restraining its enhancement of ABl1 *** together,our study reveals that RAF22,ABl1,and OST1 form a dynamic regulatory network that plays crucial roles in optimizing plant growth and environmental adaptation under drought stress.

关键词： ABA signaling RAF22 ABl1 OST1 phosphorylation

Functional analysis of the ABA-responsive protein family in ABA and stress signal transduction in Arabidopsis

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Chinese Science Bulletin 2013年第31期58卷 3721-3730页

作者： LIU LingYun LI Na YAO chunpeng MENG SaSa song chunpeng State Key Laboratory of Cotton Biology Laboratory of Plant Stress Biology Department of Biology Henan University Zhengzhou Shuqing Medical College Faculty of Basic Medicine

The phytohormone abscisic acid(ABA)plays an important role in plant growth and development,for example in seed dormancy and germination,as well as in plant responses to environmental stresses,such as drought and high *** studies have shown that ABA regulates the expression of genes with an ABA-responsive element(ABRE)and their corresponding physiological *** analysis identified a GRAM domain-containing gene family that has a multiple ABRE cis-element,which was termed the ABA-responsive protein(ABR)*** analyze the function of the ABR family,we identified homozygous T-DNA insertion mutants and constructed abr1,2,3 double mutants and triple *** abr1,abr2 and abr3single mutants showed a normal phenotype;however,the germination of seeds of the double mutants and triple mutant were insensitive to ABA,NaCl,mannitol and ***1-GFP was distributed as a punctate structure in the cytosol and may be localized in the endomembrane *** ABR2-GFP and ABR3-GFP proteins localized in the *** addition,ABR1,ABR2 and ABR3 were expressed in various tissues,and could be induced by several abiotic stresses,especially by *** expressions of these genes were significantly suppressed in aba2,abi1 and abi2 null *** results suggested that the ABR family may act downstream of ABI1 and ABI2 in the ABA signal transduction process in plants.

关键词： ABA调节基因家族非生物胁迫蛋白定位信号转导生理反应拟南芥植物生长发育

Arabidopsis calcium-dependent protein kinase CPK28 is potentially involved in the response to osmotic stress

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Chinese Science Bulletin 2014年第11期59卷 1113-1122页

作者： Anli Gao Qingyang Wu Yu Zhang Yuchen Miao chunpeng song State Key Laboratory of Cotton Biology Institute of Plant Stress BiologyCollege of Life SciencesHenan University

As calcium sensors, plant calcium-dependent protein kinases(CDPKs) play important roles in plants' responses to various abiotic stresses. Here, we report the functional characterization of CPK28, a member of the CDPK family in Arabidopsis, in response to osmotic *** cpk28 mutant, a loss-of-function mutant, exhibited an NaCl- and mannitol-sensitive phenotype in green cotyledons, while CPK28-overexpressing plants displayed stronger tolerance to NaCl and mannitol stresses than wildtype plants. Reverse transcriptase-polymerase chain reaction and beta-glucuronidase staining assays showed that NaCl and mannitol stresses induced CPK28. CPK28-overexpressing lines accumulated significantly more proline relative to wild-type plants and mutant plants under NaCl and mannitol stresses. Transient expression of CPK28-GFP in mesophyll cell protoplasts, as well as stable transgenic lines expressing CPK28-GFP, showed that CPK28 was localized in the plasma membrane. Expression levels of known stress-responsive genes were not significantly altered in the null mutant and overexpression lines,suggesting that CPK28 possibly mediated the stress response via the regulation of target proteins rather than via regulation at the level of transcription. Meanwhile, CPK28could autophosphorylate. Taken together, these data demonstrated that CPK28, a potential positive regulator, is involved in the response to osmotic stress in Arabidopsis.

关键词：钙依赖蛋白激酶渗透胁迫拟南芥聚合酶链反应 CDPK 应激反应转录水平非生物胁迫

Biological function analysis of the phosphorylation sites for Arabidopsis CAP1

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Science Bulletin 2017年第11期62卷 761-763页

作者： Yun Zhou Lu Zhang Zhangyun Wu Mengmeng Dai Luying Li Ling Bai chunpeng song Institute of Plant Stress Biology State Key Laboratory of Cotton Biology Department of Biology Henan University Kaifeng 475001 China

Cells need to respond successfully to ever-changing environmental conditions to maintain normal *** is achieved through various signal transduction ***-like kinases(RLKs)are involved in many aspects of the growth and ... 详细信息

关键词：磷酸化位点拟南芥生物学功能植物生长发育类受体蛋白激酶胞外结构域信号转导受体激酶

Generation of the genetic mutant population for the screening and characterization of the mutants in response to drought in maize

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Chinese Science Bulletin 2014年第8期59卷 766-775页

作者： Zhiyong Gao Hao Liu Hongliang Wang Ning Li Daojie Wang Yuwei song Yuchen Miao chunpeng song State Key Laboratory of Cotton Biology Laboratory of Plant Stress Biology Department of Biology Henan University

The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the *** we statistically analyzed these candidate *** drought stress,we screened seedlings at 3-leaf stage by using the infrared thermography,and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines,which temperature were significant different with *** candidate mutants were primarily analyzed by infrared thermography,chlorophyll fluorescence,leaf water losing,photosynthetic characteristics,content of soluble sugars,soluble protein,and proline *** selected mutants were cloned by MuTAIL–PCR *** we provide the better genetic materials for research on maize breeding for drought tolerance.

关键词：干旱胁迫玉米育种突变体基因突变红外热成像鉴定筛选群体

Reciprocal regulation between the negative regulator PP2CG1 phosphatase and the positive regulator OST1 kinase confers cold response in Arabidopsis

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Journal of Integrative Plant Biology 2021年第8期63卷 1568-1587页

作者： Jian Lv Jingyan Liu Yuhang Ming Yiting Shi chunpeng song Zhizhong Gong Shuhua Yang Yanglin Ding State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing100193 China Institute of Plant Stress Biology Collaborative Innovation Center of Crop Stress BiologyHenan UniversityKaifeng475001 China

Protein phosphorylation and dephosphorylation have been reported to play important roles in plant cold *** addition,phospho-regulatory feedback is a conserved mechanism for biological processes and stress responses in animals and ***,it is less well known that a regulatory feedback loop is formed by the protein kinase and the protein phosphatase in plant responses to cold ***,we report that OPEN STOMATA 1(OST1)and PROTEIN PHOSPHATASE 2C G GROUP 1(PP2CG1)reciprocally regulate the activity during the cold stress *** interaction of PP2CG1 and OST1 is inhibited by cold stress,which results in the release of OST1 at the cytoplasm and nucleus from suppression by ***,cold-activated OST1 phosphorylates PP2CG1 to suppress its phosphatase activity,thereby amplifying cold signaling in *** of PP2CG1 and its homolog PP2CG2 enhance freezing tolerance,whereas overexpression of PP2CG1 decreases freezing ***,PP2CG1 negatively regulates protein levels of C-REPEAT BINDING FACTORs(CBFs)under cold *** results uncover a phosphor/dephosphor-regulatory feedback loop mediated by PP2CG1 phosphatase and OST1 protein kinase in plant cold responses.

关键词： Arabidopsis feedback loop freezing stress protein kinase OST1 protein phosphatases PP2CG1/2

Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses

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Chinese Science Bulletin 2006年第21期51卷 2586-2594页

作者： song Yuwei KANG Yanli LIU Hao ZHAO Xiaoliang WANG Pengtao AN Guoyong ZHOU Yun MIAO Chen song chunpeng Laboratory of Plant Stress Biology Department of Biology Henan University Kaifeng 475001 China

In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appro- priate state. However, many signaling components in guard cell signaling remain elusive. In our laboratory, a tool for non-invasive remote infrared thermal images was used to screen an ethyl methane sulfonate-mutagenized population for Arabidopsis stomatal response mutants under multiple stresses (ABA, H2O2, CO2, etc.). More than forty "hot" or "cold" mutants were isolated (above or below 0.5℃ in con- trast to normal plantlets). Identification and primary genetic analysis of these mutants show that they are monogenic recessive mutations and there exist distinct difference in stomata apertures compared to wild type. These mutants in response to various environmental stresses and hormones were comprehen- sively investigated, which enables us to further un- derstand the cross-talk in different signal transduction pathways.

关键词：红外摄象机保卫细胞遗传分析信号转导变异