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Comparison of Microdroplet Digital PCR Assays with Real-time Fluorescence Quantitative PCR for Clostridioides difficile Detection

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Biomedical and Environmental Sciences 2023年第7期36卷 653-657页

作者： YIN Cai Hong song zhan yun LIU Xing Xing WANG Xiao Mu WANG Ying GAO Cheng Cheng song Xiu Ling FENG Xin College of Public Health Jilin UniversityChangchun 130021JilinChina Changchun customs Technology Center Changchun130062JilinChina CollegeofVeterinaryMedicine JilinUniversityChangchun130062JilinChina College of Basic Medical Sciences Jilin UniversityChangchun 130021JilinChina

Clostridioides difficile(***),is an anaerobic Gram-positive bacterium found in the human *** use of antibacterial drugs can result in *** infection(CDI)[1],which is one of the most important hospital-acquired diseases affecting all ***-and-culture-based identification and the cell cytotoxicity neutralization assay(CCNA)are considered the“gold standard”for detecting toxins or pathogenic strains[2],with long and time-consuming incubation *** quantitative polymerase chain reaction(PCR)is commonly used in clinical laboratory tests[3].However,the sensitivity,accuracy,and resolution of detection are limited by low-copy target gene molecules and subtle differences in template *** *** is distributed in feces in the natural environment and the sample matrix is complex,it is difficult to detect pathogens at low *** quantifying *** and implementing effective measures to combat *** infections are of utmost importance.

关键词： intestine drugs template

Enhanced memristor performance via coupling effect of oxygen vacancy and ferroelectric polarization

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Journal of Materials Science & Technology 2024年第4期171卷 139-146页

作者： Zhi yun Yue Zhi Dong zhang zhan Jie Wang Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China

As a new type of nonvolatile memory,the resistive memristor has broad application prospects in in-formation storage and neural computing based on its excellent resistive switching(RS)*** present,it is still a great challenge to improve both ferroelectric polarization and leakage current to achieve a high RS on/offratio of ferroelectric ***,epitaxial Pb(Zr_(0.40)Ti_(0.60))O_(3)(PZT)thin films with low content Ca doping were deposited on the Nb:SrTiO_(3)substrate to prepare PCZT/NSTO het-erostructures and their RS behaviors were *** research findings show that compared with pure PZT film,the ferroelectric polarization of 1-mol%-Ca-doped PZT film is slightly improved,while the leak-age current is increased by three orders of ***,the RS on/offratio reaches 2.5×10^(5),about three orders of magnitude higher than pure PZT *** theoretical analysis reveals that the RS behavior of PCZT/NSTO heterostructures is controlled by the PCZT/NSTO interfacial barrier and the space charge-limited current *** results demonstrate that the ferroelectricity and electricity of ferroelectric thin films can be improved simultaneously by doping low-content Ca ions to increase the RS performance,which provides a good reference for the development of high-performance ferroelectric memristor devices.

关键词： Ferroelectric memristor Ca-doped PZT Ferroelectric polarization Oxygen vacancies Resistive switching

Stabilizing iridium sites via interface and reconstruction regulations for water oxidation in alkaline and acidic media

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Journal of Energy Chemistry 2024年第2期89卷 355-363,I0009页

作者： Weibin Chen Yanhui song Lei Li Junjie Guo zhan Lin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light IndustryGuangdong University of TechnologyGuangzhou 510006GuangdongChina Key Laboratory of Interface Science and Engineering in AdvancedMaterials Ministry of EducationTaiyuan University of TechnologyTaiyuan 030024ShanxiChina

Exploring effective iridium(Ir)-based electrocatalysts with stable iridium centers is highly desirable for oxygen evolution reaction(OER).Herein,we regulated the incorporation manner of Ir in Co_(3)O_(4)support to stabilize the Ir sites for effective *** anchored on the surface of Co_(3)O_(4)in the form of Ir(OH)_6 species,the created Ir-OH-Co interface leads to a limited stability and poor acidic OER due to Ir *** doped into Co_(3)O_(4)lattice,the analyses of X-ray absorption spectroscopy,in-situ Raman,and OER measurements show that the partially replacement of Co in Co_(3)O_(4)by Ir atoms inclines to cause strong electronic effect and activate lattice oxygen in the presence of Ir-O-Co interface,and simultaneously master the reconstruction effect to mitigate Ir dissolution,realizing the improved OER activity and stability in alkaline and acidic *** a result,Ir_(lat)@Co_(3)O_(4)with Ir loading of 3.67 wt%requires 294±4 mV/285±3 mV and 326±2 mV to deliver 10 mA cm^(-2)in alkaline(0.1 M KOH/1.0 M KOH)and acidic(0.5 M H_(2)SO_(4))solution,respectively,with good stability.

关键词： Interface effect Reconstruction Ir dissolution Ir-O-Co Oxygen evolution reaction

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古琴曲中的渔樵主题及其文化意蕴

艺术百家 2020年第1期36卷 72-77,89页

作者：宋展云扬州大学文学院江苏扬州225009

古琴曲中的渔樵主题有其历时性的演变过程,琴曲在充分吸收前代渔樵母题的基础上,被赋予更多的生命思索和价值判断。《渔歌》体现出"逍遥物外"的人生追求,《樵歌》被赋予更多的"招隐"意味,《渔樵问答》蕴含着历史兴衰之感。这三首琴曲音... 详细信息

古琴曲中的渔樵主题有其历时性的演变过程,琴曲在充分吸收前代渔樵母题的基础上,被赋予更多的生命思索和价值判断。《渔歌》体现出"逍遥物外"的人生追求,《樵歌》被赋予更多的"招隐"意味,《渔樵问答》蕴含着历史兴衰之感。这三首琴曲音律潇洒、结构精微、技法高妙,生动再现出渔樵形象的生存空间与情感寄托。琴曲中的渔樵主题对渔樵母题有所继承与扩充,使得渔樵文化富有更为普遍和持久的生命力。渔樵主题琴曲塑造出的隐逸形象,使古琴艺术与隐逸文化的关联更为密切,成为古代士人精神寄托、性情所注的人格典范。对于琴曲文化母题源流与演变的探析,有助于加深古琴艺术史的研究,且利于建构多元化的艺术史理论与文化史研究范式。

关键词：古琴曲渔樵文化母题隐逸文化

《文选集注》中江淹杂体诗的研究价值——兼论先唐文本的研究方法

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上海大学学报（社会科学版） 2018年第3期35卷 44-55页

作者：宋展云扬州大学文学院江苏扬州220009

先唐经典文本具有不稳定性和衍生性,需要利用诸多版本及注本的比较与互参进行综合研究。以《文选集注·江文通杂体诗》为例,其序文及注解、正文的异文、篇题的变化、李善注及各家注本的多维诠解、注本所引亡佚文献等,不仅对于勘定原文... 详细信息

先唐经典文本具有不稳定性和衍生性,需要利用诸多版本及注本的比较与互参进行综合研究。以《文选集注·江文通杂体诗》为例,其序文及注解、正文的异文、篇题的变化、李善注及各家注本的多维诠解、注本所引亡佚文献等,不仅对于勘定原文、补充文学史料具有意义,而且对于探寻汉晋诗歌艺术风格及其经典化进程也颇有裨益。通过《文选》早期钞本、刻本的比勘,可以尽量追溯文本的原貌,利于整理并校订中古集部文献,同时也应当尊重文本的开放性及多样性,注意文本形态流变背后的深层文化内涵。关注文本流变及其生成机制,充分利用集注本及历代注评本,并从多元开放的视角重新审视正文、注文及读者之间的互动关系,对于古代文学研究具有方法论意义。

关键词：《文选集注》江淹《杂体诗》文本形态经典化

Calculation of Mass Concrete Temperature and Creep Stress under the Influence of Local Air Heat Transfer

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 2977-3000页

作者： Heng zhang Chao Su Xiaohu Chen Zhizhong song Weijie zhan Academy of Science and Technology Changjiang Institute of Survey Planning Design and ResearchWuhan430010China College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing210098China Chief Engineer Office Wujiang Company of Goupitan Power Construction CooperationYuqing564408China

Temperature-induced cracking during the construction of mass concrete is a significant *** simulations of concrete temperature have primarily assumed that the concrete is placed in an open *** problem of heat transfer between the air and concrete has been simplified to the concrete’s heat dissipation ***,in the case of tubular concrete structures,where air inlet and outlet are relatively limited,the internal air temperature does not dissipate promptly to the external environment as it *** accurately simulate the temperature and creep stress in tubular concrete structures with enclosed air spaces during construction,we establish an air–concrete coupled heat transfer model according to the principles of conjugate heat transfer,and the accuracy of the model is verified through ***,we conduct a case study to analyze the impact of airflow within the ship lock corridor on concrete temperature and creep *** results demonstrate that enhancing airflow within the corridor can significantly reduce the maximum concrete *** with cases in which airflow within the corridor is neglected,the maximum concrete temperature and maximum tensile stress can be reduced by 12.5℃ and 0.7 MPa,respectively,under a wind speed of 4 m/*** results of the traditional calculation method are relatively close to those obtained at a wind speed of 1 m/***,the temperature reduction process in the traditional method is faster,and the method yields greater tensile stress values for the corridor location.

关键词： Conjugate heat transfer temperature field mass concrete creep stress

Preparation and combustion properties of laminated sticks of B-CuO and B-Bi_(2)O_(3)

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Defence Technology（防务技术） 2024年第8期38卷 67-74页

作者： Haoyu song Chenyang Li Fubing Gao Chongwei An Shijiao Li Xuan zhan Jianchen He School of Environment and Safety Engineering North University of ChinaTaiyuan 030051China Chongqing Military Agency of the Army Chongqing 400060China

To explore the composite process of B-CuO and B-Bi_(2)O_(3) two-component laminated sticks,obtain the corresponding sticks with good printing effect,and explore the energy release *** this study,boron,copper oxide,and bismuth trioxide powders were dispersed in the dispersed phase (DMF) using F_(2602) as a binder,and the construction of two-component B-CuO,B-Bi_(2)O_(3),three-component microcomposite,and three-component macro-composite sticks were realized with the help of double nozzle direct ink writing (DIW) technique *** resulting sticks were ignited by a nichrome wire energized with a direct current,and a high-speed camera system was used to record the combustion behavior of the sticks,mark the flame position,and calculate the rate of *** results showed that the B-CuO stick burning rate (42.11 mm·s^(-1)) was much higher than that of B-Bi_(2)O_(3)(17.84 mm·s^(-1)).The formulation with the highest CuO content (ω_(CuO)=58.7%) in the microscale composite of the sticks also had the fastest burning rate of 60.59 mm·s^(-1),as the CuO content decreased (ω_(CuO)=43.5%,29.3%),its burning rate decreased to 34.78 mm·s^(-1),37.97 mm·s^(-1).The stick with the highest copper oxide content(ω_(CuO)=60%) also possessed the highest burning rate (48.84 mm·s^(-1)) in the macro-composite sticks,and the burning rates of the macro-composite sticks with component spacing of 0.1 mm,0.2 mm,and 0.5 mm were 43.34 mm·s^(-1),48.84 mm·s^(-1),and 40.76 mm·s^(-1).

关键词： Boron-based thermite Direct ink writing Linear burning rate Multi-component composite

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《文选》鸟兽赋之寒士心态探微

广西师范大学学报（哲学社会科学版） 2015年第1期51卷 112-117页

作者：宋展云中国社会科学院文学研究所北京100732 扬州大学文学院江苏扬州225002

《文选》所录五篇鸟兽类赋作是汉魏六朝时期此类作品的名篇。其作者大多出身寒微,他们在赋作中通过鸟兽形象表达寒士心声、抒发情志。这些作品饱含着寒族文士避祸自守、明德善终、追求自由的精神寄托,在体物手法上也丰富多彩,达到了物... 详细信息

《文选》所录五篇鸟兽类赋作是汉魏六朝时期此类作品的名篇。其作者大多出身寒微,他们在赋作中通过鸟兽形象表达寒士心声、抒发情志。这些作品饱含着寒族文士避祸自守、明德善终、追求自由的精神寄托,在体物手法上也丰富多彩,达到了物我融合的艺术境界。出身寒门、身处乱世、昧于自处等原因最终导致了寒族文士的人生悲剧。寒族士人笔下的鸟兽群像,融入了他们的人生体验和身世感伤,为中国文学增添了个性与张力,形成了与士族文学不同的风貌。

关键词：《文选》鸟兽赋寒士心态

The role of inflammasomes in human diseases and their potential as therapeutic targets

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Signal Transduction and Targeted Therapy 2024年第2期9卷 479-508页

作者： Jing Yao Keenan Sterling Zhe Wang yun zhang Weihong song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijing 100053China Townsend Family Laboratories Department of PsychiatryBrain Research CenterThe University of British Columbia2255 Wesbrook MallVancouverBC V6T 1Z3Canada Key Laboratory of Neurodegenerative Diseases Ministry of EducationBeijingP.R.China Zhejiang Clinical Research Center for Mental Disorders Key Laboratory of Alzheimer’s Disease of Zhejiang ProvinceSchool of Mental Health and The Affiliated Kangning HospitalInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang 325000China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang 325000China

Inflammasomes are large protein complexes that play a major role in sensing inflammatory signals and triggering the innate immune *** inflammasome complex has three major components:an upstream sensor molecule that is connected to a downstream effector protein such as caspase-1 through the adapter protein *** formation typically occurs in response to infectious agents or cellular *** active inflammasome then triggers caspase-1 activation,followed by the secretion of pro-inflammatory cytokines and pyroptotic cell *** inflammasome activation and activity contribute to the development of diabetes,cancer,and several cardiovascular and neurodegenerative *** a result,recent research has increasingly focused on investigating the mechanisms that regulate inflammasome assembly and activation,as well as the potential of targeting inflammasomes to treat various *** clinical trials are currently underway to evaluate the therapeutic potential of several distinct inflammasome-targeting ***,understanding how different inflammasomes contribute to disease pathology may have significant implications for developing novel therapeutic *** this article,we provide a summary of the biological and pathological roles of inflammasomes in health and *** also highlight key evidence that suggests targeting inflammasomes could be a novel strategy for developing new disease-modifying therapies that may be effective in several conditions.

关键词： inflam therapeutic potential