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P7C3-A20 treats traumatic brain injury in rats by inhibiting excessive autophagy and apoptosis

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Neural Regeneration Research 2024年第5期19卷 1078-1083页

作者： Zhiqing Yang Zhenchao Wang Xiaoqi Deng Lingxin Zhu Zhaomeng song Changyu Cao xinran Li School of Life Science and Engineering Foshan UniversityFoshanGuangdong ProvinceChina Foshan University Veterinary Teaching Hospital FoshanGuangdong ProvinceChina

Traumatic brain injury is a severe health problem leading to autophagy and apoptosis in the brain.3,6-Dibromo-beta-fluoro-N-(3-methoxyphenyl)-9H-carbazole-9-propanamine(P7C3-A20)can be neuroprotective in various diseases,including ischemic stroke and neurodegenerative ***,whether P7C3-A20 has a therapeutic effect on traumatic brain injury and its possible molecular mechanisms are ***,in the present study,we investigated the therapeutic effects of P7C3-A20 on traumatic brain injury and explored the putative underlying molecular *** established a traumatic brain injury rat model using a modified weight drop method.P7C3-A20 or vehicle was injected intraperitoneally after traumatic brain *** neurological deficits were found in rats after traumatic brain injury,with deterioration in balance,walking function,and learning ***,hematoxylin and eosin staining showed significant neuronal cell damage,while terminal deoxynucleotidyl transferase mediated dUTP nick end labeling staining indicated a high rate of *** presence of autolysosomes was observed using transmission electron microscope.P7C3-A20 treatment reversed these pathological *** blotting showed that P7C3-A20 treatment reduced microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ)autophagy protein,apoptosis-related proteins(namely,Bcl-2/adenovirus E1B 19-kDa-interacting protein 3[BNIP3],and Bcl-2 associated x protein[Bax]),and elevated ubiquitin-binding protein p62(p62)autophagy protein ***,P7C3-A20 can treat traumatic brain injury in rats by inhibiting excessive autophagy and apoptosis.

关键词： apoptosis autophagy cortex hippocampus motor function P7C3-A20 traumatic brain injury

百日咳毒素兔多克隆抗体的制备及其ELISA定量检测方法的建立和验证

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中国生物制品学杂志 2024年第3期37卷 350-355页

作者：宋欣然张茗婧伊丽男彭少丹王帆张国强司伟雪朱涛天津科技大学生物工程学院天津300457 康希诺生物股份公司天津300301

目的制备百日咳毒素(pertussis toxin,PT)兔多克隆抗体(简称多抗),建立定量检测PT抗原含量的双抗体夹心ELISA法,并进行验证及应用。方法采用传统方法,用PT免疫青紫蓝兔制备PT兔多抗。优化ELISA系统反应条件,建立PT双抗体夹心ELISA定量... 详细信息

目的制备百日咳毒素(pertussis toxin,PT)兔多克隆抗体(简称多抗),建立定量检测PT抗原含量的双抗体夹心ELISA法,并进行验证及应用。方法采用传统方法,用PT免疫青紫蓝兔制备PT兔多抗。优化ELISA系统反应条件,建立PT双抗体夹心ELISA定量检测方法,并进行特异性、线性、准确性、精密性、灵敏度验证。采用建立的方法对脱毒过程样品及其他百日咳抗原纯化过程样品菌毛蛋白(fimbriae proteins,FIM)原液中PT抗原含量进行检测。结果PT抗原含量双抗体夹心ELISA检测方法的工作条件为PT兔多抗包被浓度为1μg/mL,酶标抗体稀释度为1∶8000。此检测系统能与PT纯化蛋白发生特异性反应,而与百日咳丝状血凝素、白喉类毒素、破伤风类毒素均未发生交叉反应;建立的双抗体夹心ELISA法线性检测范围在25~400 ng/mL之间;PT高、中、低浓度回收率分别为103.27%、91.48%、103.52%;酶标板内和板间的变异系数(CV)均小于10%;该方法的灵敏度为20.719 ng/mL,检测下限为41.438 ng/mL。检测5种不同脱毒工艺条件下、不同取样时间点的35批样品,其抗原含量变化均符合脱毒反应变化趋势。结论成功制备了PT兔多抗,并建立了精密性和准确性良好的定量检测PT抗原含量的双抗体夹心ELISA法,可用于组分百白破联合疫苗生产过程中化学脱毒样品的抗原含量检测。

关键词：百日咳毒素多克隆抗体酶联免疫吸附试验

中国网约车监管政策变迁研究——以倡议联盟框架为分析视角

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中国行政管理 2017年第6期 103-107页

作者：宋心然首都经济贸易大学城市经济与公共管理学院

倡议联盟框架(ACF)旨在研究复杂的长期性政策变迁,将政策的变动视为政策联盟和外部事件共同作用的结果。本文运用这一理论分析历时不长的中国网约车监管政策的变迁,证明了倡议联盟框架的诸多命题在分析中国的政策过程中同样适用。但是,... 详细信息

倡议联盟框架(ACF)旨在研究复杂的长期性政策变迁,将政策的变动视为政策联盟和外部事件共同作用的结果。本文运用这一理论分析历时不长的中国网约车监管政策的变迁,证明了倡议联盟框架的诸多命题在分析中国的政策过程中同样适用。但是,作为短期政策的案例应用,我们在研究的过程中有新的发现,在核心信仰不发生改变的前提下,政策变迁也有可能发生;由于核心信仰未改变,政策极易变动;立法部门化对政策变迁影响显著;"互联网+"技术的应用,大大加速了政策变迁的进程。

关键词：倡议联盟框架网约车监管政策变迁互联网+

Transdermal delivery of interleukin-12 gene targeting dendritic cells enhances the anti-tumour effect of programmed cell death protein 1 monoclonal antibody

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Biomaterials Translational 2021年第2期2卷 151-164页

作者： Huoyan Hong Xiaoyun Wang xinran song Gomaa El Fawal Kaili Wang Di Jiang Yifei Pei Zhe Wang Hongsheng Wang Shanghai Engineering Research Centre of Nano-Biomaterials and Regenerative Medicine Key Laboratory of Science&Technology of Eco-Textile(Ministry of Education)College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghaiChina Department of Obstetrics&Gynaecology Shanghai First People’s Hospital Affiliated to Shanghai Jiao Tong UniversityShanghaiChina Department of Polymer Materials Research Advanced Technology and New Materials Research InstituteScientific Research and Technological Applications City(SRTA-City)New Borg El-Arab CityAlexandriaEgypt

Recent studies have suggested that the anti-tumour effect of the programmed cell death protein 1 monoclonal antibody(aPD-1)depends on the expression of interleukin-12(IL-12)by dendritic cells(DCs).Since DCs are abundant in skin tissues,transdermal delivery of IL-12 targeting DCs may significantly improve the anti-tumour effect of *** this study,a novel mannosylated chitosan(MC)-modified ethosome(Eth-MC)was obtained through electrostatic *** Eth-MC loaded with plasmid containing the IL-12 gene(pIL-12@Eth-MC)stimulated DCs to express mature-related molecular markers such as CD86,CD80,and major histocompatibility complex-II in a targeted *** pIL-12@Eth-MC was then mixed with polyvinyl pyrrolidone solution to make microspheres using the electrospray technique,and sprayed onto the surface of electrospun silk fibroin-polyvinyl alcohol nanofibres to obtain a PVP-pIL-12@Eth-MC/silk fibroin-polyvinyl alcohol composite nanofibrous patch(termed a transcutaneous immunization(TCI)patch).The TCI patch showed a good performance on transdermal drug *** experiments on melanoma-bearing mice showed that topical application of the TCI patches promoted the expression of IL-12 and inhibited the growth of ***,combined application of the TCI patch and aPD-1 showed a stronger anti-tumour effect than aPD-1 *** combination therapy significantly promoted the expression of IL-12,interferon-γand tumour necrosis factor-α,the infiltration of CD4+and CD8+T cells into tumour tissues,and thus promoted the apoptosis of tumour *** present study provides a convenient and non-invasive strategy for improving the efficacy of immune checkpoint inhibitor *** study was approved by the Institutional Animal Care and Use Committee at Donghua University(approval ***-NSFC-2020-11)on March 31,2020.

关键词： aPD-1 dendritic cells ethosome IL-12 transcutaneous immunization

Genome-wide identification of the CONSTANS-LIKE(COL)family and mechanism of fruit senescence regulation by PpCOL8 in sand pear(Pyrus pyrifolia)

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Journal of Integrative Agriculture 2024年第4期23卷 1222-1237页

作者： Yue Xu Shurui song Huiying Wang Xilong Cao xinran Zhao Wenli Wang Liyue Huo Yawei Li Misganaw Wassie Bin Lu Liang Chen Haiyan Shi College of Horticulture Hebei Agricultural UniversityBaoding 071001China Wuhan Botanical Garden Chinese Academy of SciencesWuhan 430074China

Pyrus pyrifolia Nakai‘Whangkeumbae'is a sand pear fruit with excellent nutritional quality and ***,the industrial development of pear fruit is significantly limited by its short shelf *** acid(SA),a well-known phytohormone,can delay fruit senescence and improve shelf ***,the mechanism by which SA regulates CONSTANS-LIKE genes(COLs)during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly *** this study,22 COL genes were identified in sand pear,including four COLs(Pp COL8,Pp COL9a,Pp COL9b,and Pp COL14)identified via transcriptome analysis and 18 COLs through genome-wide *** COL genes were divided into three subgroups according to the structural domains of the COL *** COL8,with two B-box motifs and one CCT domain,belonged to the first *** contrast,the other three Pp COLs,Pp COL9a,Pp COL9b,and Pp COL14,with similar conserved protein domains and gene structures,were assigned to the third *** four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit ***,the expression of Pp COL8 was inhibited by exogenous SA treatment,while SA up-regulated the expression of Pp COL9a and Pp ***,Pp COL8 interacts with Pp MADS,a MADS-box protein preferentially expressed in fruit,and SA up-regulated its *** the production of ethylene and the content of malondialdehyde(MDA)were increased in Pp COL8-overexpression sand pear fruit,the antioxidant enzyme(POD and SOD)activity and the expression of Pp POD1 and Pp SOD1 in the sand pear fruits were down-regulated,which showed that Pp COL8 promoted sand pear fruit *** contrast,the corresponding changes were the opposite in Pp MADS-overexpression sand pear fruits,suggesting that Pp MADS delayed sand pear fruit *** co-transformation of Pp COL8 and Pp MADS also delayed sand pear fruit *** re

关键词： Pyrus pyrifolia CONSTANS-LIKE gene salicylic acid fruit senescence MADS

Folic Acid-Polyethyleneimine-Ethosome as a Potential Tumor Targeting Carrier: Preparation, Characterization and in vitro Evaluation

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Journal of Donghua University(English Edition) 2019年第5期36卷 437-444页

作者： song xinran MA Linlin GOMAA Elfawal HONG Huoyan LIANG Jingtian SUN Meiqi ZHANG Lin WANG Hongsheng Key Laboratory of Science&Technology of Eco-Textile Ministry of EducationShanghai 201620China College of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China Advanced Technology and New Materials Research Institute City of Scientific Research and Technological ApplicationsAlexandriaEgypt Department of Pharmacy Shaoxing People's HospitalShaoxing Hospital of Zhejiang UniversityShaoxing 312000China

A novel composite carrier of folic acid(FA)-polyethyleneimine(PEI)-ethosome(Eth)(FA-PEI-Eth)was developed for the treatment of cancers through loading and targeting delivery of multidrug(including gene and other drugs)into cancer *** and chemical property tests were done to prove the grafting of the *** retardation test was done to determine the optimal ratio of DNA@PEI complex,and cytocompatibility tests and tumor cell uptake tests were done to evaluate the efficiency of the *** results demonstrated that the FA-PEI-Eth could effectively deliver a gene and other drugs into tumor cells simultaneously,and suggested that this composite would be a promising carrier in tumor-targeted therapy applications.

关键词： ethsome folic acid polyethylenimine(PEI) tumor-targeted therapy

A Survey of Gesture Recognition Using Frequency Modulated Continuous Wave Radar

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Journal of Computer and Communications 2024年第6期12卷 115-134页

作者： xinran Qiu Junhao Liu Lulu song Haofei Teng Jiaqi Zhang Zhengjie Wang College of Electronic and Information Engineering Shandong University of Science and Technology Qingdao China

With technology advances and human requirements increasing, human-computer interaction plays an important role in our daily lives. Among these interactions, gesture-based recognition offers a natural and intuitive user experience that does not require physical contact and is becoming increasingly prevalent across various fields. Gesture recognition systems based on Frequency Modulated Continuous Wave (FMCW) millimeter-wave radar are receiving widespread attention due to their ability to operate without wearable sensors, their robustness to environmental factors, and the excellent penetrative ability of radar signals. This paper first reviews the current main gesture recognition applications. Subsequently, we introduce the system of gesture recognition based on FMCW radar and provide a general framework for gesture recognition, including gesture data acquisition, data preprocessing, and classification methods. We then discuss typical applications of gesture recognition systems and summarize the performance of these systems in terms of experimental environment, signal acquisition, signal processing, and classification methods. Specifically, we focus our study on four typical gesture recognition systems, including air-writing recognition, gesture command recognition, sign language recognition, and text input recognition. Finally, this paper addresses the challenges and unresolved problems in FMCW radar-based gesture recognition and provides insights into potential future research directions.

关键词： Millimeter-Wave Radar Gesture Recognition Human-Computer Interaction Feature Extraction

我国金融网络安全韧性监管研究:现实困境、英国经验及制度构建

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情报杂志 2022年第3期41卷 80-86,94页

作者：宋心然李鹏飞张丽迎首都经济贸易大学城市经济与公共管理学院北京100070

[研究目的]新技术与金融行业的结合不仅催生出新的金融产品,也不可避免地带来了新的金融网络安全问题。如何在鼓励金融从业机构创新发展的同时,防控金融网络风险,完善金融网络安全监管,就成为各国金融监管部门面临的重要问题。英国的“... 详细信息

[研究目的]新技术与金融行业的结合不仅催生出新的金融产品,也不可避免地带来了新的金融网络安全问题。如何在鼓励金融从业机构创新发展的同时,防控金融网络风险,完善金融网络安全监管,就成为各国金融监管部门面临的重要问题。英国的“渗透性韧性测试”是兼顾金融从业机构创新与金融网络安全的有效机制,研究其运作特点可为我国金融网络安全监管提供有益思路。[研究方法]通过分析我国金融网络安全监管面临的困境,着重研究英国“渗透性韧性测试”机制的建设历程、具体运作模式与本质特征,分析我国在金融网络监管层面引入渗透性韧性测试的可行性。[研究结论]对英国金融网络安全韧性监管的模式进行本土化借鉴,即加强金融网络安全监管的合作治理,在重构监管组织体系的基础上,完善相关激励机制,提高各参与主体抵御风险的专业能力,推进传统金融网络安全监管向金融网络安全韧性监管防控体系方向嬗变。

关键词：网络安全金融监管韧性监管金融科技渗透性韧性测试英国

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基于适老化设计理论的智慧医疗产品设计研究

艺术科技 2023年第16期36卷 162-164页

作者：宋欣冉福州大学福建厦门361024

随着人口老龄化程度不断加深,适老化产品的设计和研发变得愈发重要。适老化产品的核心原则是关注用户的需求和体验。在设计过程中,要充分了解老年人的特点和需求,考虑到老年群体的身体和认知限制。适老化产品设计旨在满足老年人日常生... 详细信息

随着人口老龄化程度不断加深,适老化产品的设计和研发变得愈发重要。适老化产品的核心原则是关注用户的需求和体验。在设计过程中,要充分了解老年人的特点和需求,考虑到老年群体的身体和认知限制。适老化产品设计旨在满足老年人日常生活中的特殊需求,以提高他们的生活质量,促进老年人身体健康,增强他们的幸福感。随着医疗技术的进步和社会意识的增强,社会对老年人的关注日益增长,这促进了适老化产品的创新和发展。文章分析人口老龄化的现状和趋势,深入了解老年人的特点和需求,旨在研究人口老龄化背景下的智慧医疗产品设计,探讨如何解决老年人产品设计中的痛点,并在适老化设计理论的基础上,从艺术设计的角度出发,从颜色、交互和情感等多个方面探索更加贴近老年人内心情感的产品设计。通过案例分析和设计探索,为智慧医疗产品设计提供创新思路。

关键词：产品设计适老化设计情感智慧医疗