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Genome-wide Mapping of Cellular Protein–RNA Interactions Enabled by Chemical Crosslinking

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Genomics, Proteomics & Bioinformatics 2014年第2期12卷 72-78页

作者： Xiaoyu Li jinghui song Chengqi Yi State Key Laboratory of Protein and Plant Gene Research School of Life Sciences Peking University Synthetic and Functional Biomolecules Center Peking-Tsinghua Center for Life Sciences

RNA–protein interactions influence many biological processes. Identifying the binding sites of RNA-binding proteins（RBPs） remains one of the most fundamental and important challenges to the studies of such interactions. Capturing RNA and RBPs via chemical crosslinking allows stringent purification procedures that significantly remove the non-specific RNA and protein interactions. Two major types of chemical crosslinking strategies have been developed to date, i.e., UV-enabled crosslinking and enzymatic mechanism-based covalent capture. In this review, we compare such strategies and their current applications, with an emphasis on the technologies themselves rather than the biology that has been revealed. We hope such methods could benefit broader audience and also urge for the development of new methods to study RNA RBP interactions.

关键词： Protein RNA interactions High-throughput sequencing Crosslinking RNA-binding proteins Aza-IP miCLIP

Expression of multidrug resistance 1 gene and C3435T genetic polymorphism in peripheral blood of patients with intractable epilepsy

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Neural Regeneration Research 2008年第11期3卷 1269-1272页

作者： Xueping Zheng Lan Tan jinghui song Yan Wang Yanping Sun Department of Neurology the Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 Guangdong Province China Department of Neurology the Municipal Hospital of Qingdao Qingdao 266071 Shandong Province China Department of Neurology the Affiliated Hospital of Qingdao University Qingdao 266003 Shandong Province China

BACKGROUND： Increased expression of multidrug resistance 1 （MDR1） mRNA in peripheral blood of patients with intractable epilepsy is not due to epilepsy drugs, but epilepsy behavior. Monitoring MDR1 expression in peripheral blood is a target for MDR1 gene evaluation. OBJECTIVE： To investigate the influence of antiepileptic drugs and seizures on MDR expression in intractable epilepsy, and to analyze the genetic polymorphisms of C3435T in the MDRl gene. DESIGN, TIME AND SETTING： Factorial designs and comparative observations at the experimental center of the Affiliated Hospital of Qingdao Medical College, Qingdao University between October 2003 and October 2004. PARTICIPANTS： A total of 120 subjects were recruited from the epilepsy clinical department of the Affiliated Hospital of Qingdao Medical College. Four groups （n = 30） were classified according to statistical factorial design： intractable epilepsy, treatment response, no treatment, and normal control groups. METHODS： One-step semi-quantitative reverse-transcription polymerase chain reaction technology was used to test expressions of the MDR1 gene in 120 subjects. C3435T polymorphisms in intractable epilepsy group and normal control groups were analyzed by polymerase chain reaction-restriction fragment length polymorphism. MAIN OUTCOME MEASURES： Expression of MDR1 mRNA in the four groups, and C3435T genetic polymorphisms in intractable epilepsy and normal control groups. RESULTS： MDRl gene expression was increased in the intractable epilepsy group, due to the factor seizures, but not the antiepileptic drugs. However, the interaction between the two factors was not statistically significant. Of the 30 subjects in the intractable epilepsy group, the following genotypes were exhibited： 3 （10%） C/C genotype, 9 （30%） C/T genotype, and 18 （60%） T/T genotype at the site of C3435T, while 4 （13%）, 10 （33%）, and 16 （53%） subjects were determined to express these genotypes in the normal control group, respectively. C and T

关键词： genetic polymorphism intractable epilepsy MDR1 gene multidrug resistance peripheral blood P-glycoprotein

Preparation of Alum-borneol-PVP Drug-loaded Fibers by Electrospinning

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Chemical Research in Chinese Universities 2021年第3期37卷 411-418页

作者： HUANG Libing LIU Yueqi SANG Xinyu song jinghui HU Ping LIU Yong College of Mechanical and Electric Engineering Beijing University of Chemical TechnologyBeijing100029P.R.China Beijing Key Laboratory of Advanced Functional Polymer Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing100029P.R.China

The alum-borneol nanoemulsion(ABN),which combines the mineral medicine alum and the botanical medicine borneol,has been applied for approximately 40 years in the clinical treatment of burns,scalds,radiation dermatitis and shingles,and has a good curative ***,the current formula and dosage form of ABN pose problems of low borneol content and ease of precipitation,which greatly affects the efficacy of the *** this study,polyvinylpyrrolidone(PVP)was selected as a carrier mixed with different proportions of alum and borneol to produce alum-borneol-PVP fibers(ABPF)by *** results showed that the stable system with good drug dispersion was 2:3(alum:borneol).The dissolution content of borneol from the ABPF was about 80%in 4 h,which was much higher than that of the alum-borneol liquid(ABL)and *** ABPF membrane showed a more significant inhibitory effect on Staphylococcus aureus than the ABL and *** composite fiber markedly increased the drug content of borneol,which was 800 times of that in *** fiber had a higher solubility than the nanoemulsion in vitro,which is of great importance for the development of new forms for the clinical application of alum and borneol.

关键词： Drug delivery Dissolution content Bioavailability Antibacterial Fiber membrane

Study of Alkali Metal Corrosion on Heating Surfaces and Bed Material Agglomerate in Biomass-fired Fluidized Bed Boiler

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Energy and Power Engineering 2013年第4期5卷 6-14页

作者： Tuo Chen Yanfen Liao Shumei Wu Xiaoqian Ma jinghui song Electric Power Research Institute of Guangdong Power Grid Corporation Guangzhou China School of Electric Power South China University of Technology Guangzhou P.R.China

The bed material agglomeration and heating surface high-temperature Corrosion Problems of biomass-fired boiler in South China were studied in this work. The inner and outer surfaces of the corrosion sample were investigated by scanning electron microscope (SEM) with Bruker EDX and XRD. Results showed that the outer side of the corrosion sample was mainly composed of alkali chloride deposited ash, sulphide and a small amount of eutectoid;while the inner side of the corrosion sample was still mainly made up of the composition of SUS316, but added with alkali metal, oxygen, chlorine and sulphur elements, appearing as the corrosion products and eutectoid. It was thought that alkali chloride deposit and the reaction with pipe metal to generate low melting point eutectoid on the outer surfaces, or the corrosion reaction through the alkali metal sulphatization process was the main reasons leading to the damage of metal surface oxide film. Chlorine plays a role as haptoreaction in the corrosion process, and transports metal material as the form of chloride from the inner side to the outer side of the pipe surfaces by diffusion, accelerating the corrosion process. Meanwhile, the slag was studied by scanning electron microscope (SEM) with Bruker EDX, and the transformation process of slage was computationally analyzed by FACTSAGE. Results showed that the amount of alkali metal in the agglomerates was little, however, caused a great impact on severe agglomerates. The increase of temperature enhanced the conversion process of alkali metal to molten oxide, especially when the temperature was higher than760℃, the amount of molten product increased sharply. Thus, the temperature control of fluidized bed plays an important role in solving the problem of alkali metal agglomerates;it also reliefs the volatile of alkali metal into gas phase, benefiting the control of heating surface corrosion.

关键词： Biomass Combustion Generation High Temperature Corrosion Agglomerate Alkali Metal

Fault Analysis and Innovation in Slag Discharge System of 440-t/h CFB Boiler

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Electricity 2009年第1期20卷 34-37页

作者： Xu Qisheng and song jinghui Guangdong Electric Power Test and Research Institute Wang Ningling Guangdong Electric Power Test and Research Institute

CFB boilers have been widely used in China in recent years with their perfect performances in coal adaptability, load variation capability and lower pollutant emission. The No.3 135-MW CFB unit in Lianzhou Power Plant is the f irst 440-t/h series CFB unit in Guangdong Province. It f inished 72-hour trial operation in Feb. 2004 and was transferred to trial operation. During the trial operation and the next commercial operation, there were some problems happened in the boiler slag discharging system, seriously affecting the safe and reliable operation and the loading capability. After innovation, these problems have been completely solved. Hopefully the solutions may be used for reference to the units with similar problems.

关键词： CFB Fault Analysis and Innovation in Slag Discharge System of 440-t/h CFB Boiler Innovation

年轻宫颈癌患者移位卵巢血流阻力指数与内分泌功能的关系

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华中科技大学学报（医学版） 2010年第1期39卷 33-36页

作者：乔峤宋静慧内蒙古医学院第一附属医院妇产科呼和浩特010059

目的分析宫颈癌根治术并卵巢移位术术后患者的卵巢功能状况及其与血流阻力指数的关系。方法1997年8月至2004年9月间收治22例年龄26~40岁宫颈癌Ⅰa~Ⅱa期患者,均行宫颈癌根治术加单侧卵巢腹膜外移位术,按术后是否放疗分为放疗组12例,未... 详细信息

目的分析宫颈癌根治术并卵巢移位术术后患者的卵巢功能状况及其与血流阻力指数的关系。方法1997年8月至2004年9月间收治22例年龄26~40岁宫颈癌Ⅰa~Ⅱa期患者,均行宫颈癌根治术加单侧卵巢腹膜外移位术,按术后是否放疗分为放疗组12例,未放疗组10例,术后通过检测血清性激素水平,B超下测定卵巢血流阻力指数（RI）来评定移位卵巢的功能。结果放疗组术后24个月与术前相比,卵泡刺激素（FSH）升高,雌二醇（E2）明显降低,差异有统计学意义（均P〈0.01）;未放疗组术后各月与术前相比血清性激素水平差异无统计学意义（均P〉0.05）。术后24个月两组激素水平比较,放疗组FSH升高,E2降低,与未放疗组相比差异有统计学意义（均P〈0.05）。两组移位卵巢的血流阻力指数（RI）于术后24个月出现明显差异,放疗组的RI明显高于未放疗组（P〈0.05）,且放疗组RI与E2呈负相关（r=-0.901,P〈0.01）,与FSH呈正相关（r=0.895,P〈0.01）。结论年轻宫颈癌患者行宫颈癌根治术并卵巢移位术后,监测卵巢功能与RI,RI与E2呈负相关,与FSH呈正相关,因此RI可作为监测卵巢功能的重要指标,并且术后辅助放疗是影响移位卵巢RI和内分泌功能的主要因素。

关键词：子宫颈癌卵巢移位术卵巢功能血流阻力指数

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大型水轮机及其辅助设备安装技术几点进展

水力发电学报 1999年第1期18卷 95-100页

作者：宋晶辉胡鹏程葛洲坝水力发电厂葛洲坝集团机电建设公司

近几年来，大型水轮机及其辅助设备的安装技术有了较大发展：蜗壳工地水压试验、联轴螺栓火焰加热工艺、油系统管道氩弧焊封底技术。不仅可以加快施工进度，节省工程投资，而且对提高安装质量。

关键词：水轮机辅助设备安装技术水电站

利用小抽蓄提高微电网风电消纳能力的可行性研究

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电测与仪表 2021年第7期58卷 130-135页

作者：宋景慧胡春潮南方电网电力科技股份有限公司广州510600

针对新能源微网系统中风电消纳能力不足,文章将小抽蓄作为储能系统与新能源微网系统组成风抽蓄联合发电系统,有效促进风电的充分消纳、提高微电网的电能质量和提升微电网运行的经济性。构建了风电-小抽蓄联合系统,然后建立了包括各项成... 详细信息

针对新能源微网系统中风电消纳能力不足,文章将小抽蓄作为储能系统与新能源微网系统组成风抽蓄联合发电系统,有效促进风电的充分消纳、提高微电网的电能质量和提升微电网运行的经济性。构建了风电-小抽蓄联合系统,然后建立了包括各项成本和效益的综合经济效益计算模型,以最大年综合效益选择最优小抽蓄配置方案,优化调度风电和小抽蓄资源,计算不同单位容量成本下的综合效益和投资回收年限。算例表明,小抽蓄单位容量成本不大于6倍传统大抽蓄时,具有经济合理性,初步说明小抽蓄有望解决中国风电消纳问题。

关键词：风力发电风电消纳小抽蓄经济可行性

Chemical pull-down reveals dynamic pseudouridylation of the mammalian transcriptome

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Chemical pull-down reveals dynamic pseudouridylation of the ...