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Responses of Contrasting Rice (Oryza sativa L.) Genotypes to Salt Stress as Affected by Nutrient Concentrations

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Agricultural Sciences in China 2011年第2期10卷 195-206页

作者： ZHANG Zhen-hua LIU Qiang song hai-xing RONG Xiang-min Abdelbagi M Ismail College of Resources and Environmental Sciences Hunan Agricultural UniversityChangsha 410128P.R.China Crops and Environment Science Division International Rice Research InstituteMetro Manila DAPO Box 7777Philippines

The study was conducted to investigate the effects of applying different concentrations of the macronutrients K＋,Ca2＋,and Mg2＋ on the responses of contrasting rice（Oryza sativa L.） genotypes under salt stress.A solution culture experiment was conducted in a phytotron at the International Rice Research Institute（IRRI）,under controlled temperature and humidity and natural *** subjected to salt stress of 100 mmol L-1 using NaCl,the salt tolerant genotypes FL478 and IR651,accumulated less Na＋ and maintained lower ratios of Na＋/K＋,Na＋/Ca2＋,and Na＋/Mg2＋ than the sensitive genotypes IR29 and *** tolerant genotypes also had higher concentrations of K＋ in their shoots and greater root and shoot biomass and green leaf *** genotypes also maintained much lower concentration of Na＋ and lower and more favorable ratios of Na＋/K＋,Na＋/Ca2＋,and Na＋/Mg2＋ in their active and developing *** tolerance and shoot and root growth of both tolerant and sensitive genotypes were enhanced considerably when higher concentrations of Ca2＋ and Mg2＋ were applied in culture *** concentration of Na＋ and the ratios of Na＋/K＋,Na＋/Ca2＋,and Na＋/Mg2＋ in shoots also declined *** beneficial effects of higher calcium were greater than that of magnesium and application of higher concentration of K＋ seems to have minor *** to salinity in rice can therefore be considerably enhanced through proper nutrient management,by increasing the concentrations of nutrient elements that have favorable effects such as Ca2＋ and Mg2＋.Calcium is particularly more effective than both magnesium and potassium,and can be applied at relatively larger quantities in salt affected soils.

关键词： rice(Oryza sativa L.) salt stress seedling stage nutrients contents nutrients distribution genotypes

Dynamics of Nutrient Accumulation in Maize Plants Under Different Water and N Supply Conditions

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Agricultural Sciences in China 2002年第12期1卷 1350-1357页

作者： song hai-xing and LI Sheng-xiu(Northwestern Science and Technology University of Agriculture and Forestry, Yangling 712100 , P. R. China) Northwestern Science and Technology University of Agriculture and Forestry Yangling 712100 P.R.China

The dynamics of accumulations of plant dry matter, nutrient uptake and N fertilizer recovery were studied with different water and N supply, using summer maize (Zea mays L. var. Shandan9) as an indicator crop. The total dry matter (including roots) and N, P, K uptake amounts were continuously increased with plant growth, and their accumulations with time during plant-growing period were shaped in S curves that could be described by exponential regression equations. Differentiating the regression equations fitting the curves over time for first derivatives, the momentary rate was obtained of the dry matter and nutrient uptake. Results show that the dry matter and the nutrient uptake were not in the same rate at all time, but changed from one time to another. Usually, the rate increased rapidly at early stages, and gradually decreased after reaching their peak. Of N, P and K, the uptake rate of N and K was higher, and their increase and decrease were both fast while P was reversed. The time of the maximum absorptive rate appeared earlier for K, followed by N, and then by P. In any case, the maximum nutrient uptake rate appeared earlier than did the dry matter. The momentary N recovery rate was similar in trend to those of dry matter and N uptake, and its maximum recovery rate occurred almost at the same time as its maximum uptake rate. Supplemental irrigation raised the cumulative and momentary rates of N. Although water and N supplies increased dry matter and nutrient uptake rates, they did not alter their changing trends during the plant-growing period.

关键词： Maize Dynamics of nutrient accumulation Rate of nutrient absorption N recovery rate

Effects of Root-Growing Space on Its Absorbing Characteristics

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Agricultural Sciences in China 2003年第8期2卷 867-874页

作者： song hai-xing and LI Sheng-xiu(College of Resources and Environment, Northwestern Science and Technology University of Agriculture and Forestry , Shaanxi 712100 , P. R. China) College of Resources and Environment Northwestern Science and Technology University of Agriculture and Forestry Shaanxi 712100 P. R. China

Influences of root-growing space of maize upon root physiological characteristics, nutrient uptake and crop yields were studied under conditions with and without supply of water and N. Results showed that limitation of the root-growing space greatly affected root growth, decreased total root-absorbing area and TTC-reductive amounts. However, it obviously increased the root active-absorbing area, specific absorbing area(absorbing area per gram root weight)and specific active-absorbing area(actively absorbing area per gram root weight)in addition to promoting the TTC-reductive intensity. This clearly showed that plants were not passively tolerant to stress, but actively regulated their physiological metabolic processes, and strengthened their absorbing ability to increase water and nutrient uptake so that root injury by the environmental stress could be reduced. Supply of water and N stimulated root growth, increased root-absorbing area and activity. promoted nutrient uptake, and therefore increased crop yield and decreased the detrimental effects resulting from the limitation of roots-growing space.

关键词： Maize Root growth space Root-absorbing area Root activity Root redundancy

Bcl-2 siRNA联合10-羟基喜树碱治疗小鼠H22肝癌移植瘤的效果

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中华肿瘤杂志 2013年第12期35卷 892-896页

作者：胡洪华宋海星成都医学院检验医学院 610500 成都医学院检验医学院生物医学系 610500

目的探讨Bcl-2siRNA和10．羟基喜树碱（HCPT）联合对小鼠H22肝癌移植瘤的治疗作用。方法设计并合成针对Bcl-2mRNA序列的siRNA，将Bcl-2siRNA转染人小鼠H22肝癌移植瘤内并联合HCPT治疗，观察肿瘤体积和小鼠体重的变化以及小鼠的生存情况... 详细信息

目的探讨Bcl-2siRNA和10．羟基喜树碱（HCPT）联合对小鼠H22肝癌移植瘤的治疗作用。方法设计并合成针对Bcl-2mRNA序列的siRNA，将Bcl-2siRNA转染人小鼠H22肝癌移植瘤内并联合HCPT治疗，观察肿瘤体积和小鼠体重的变化以及小鼠的生存情况。对肿瘤组织进行石蜡切片，采用HE染色观察移植瘤组织的形态变化。采用逆转录聚合酶链反应（RT—PCR）检测移植瘤组织中Bcl-2mRNA的表达。采用流式细胞术检测移植瘤组织中H22细胞的细胞周期和凋亡变化。结果用药21d后，siRNA干扰联合HCPT治疗组H22荷瘤小鼠的肿瘤体积为（571．47±67．31）mm3，明显小于HCPT治疗组[（880．47±107．31）mm3，P〈0．05]、siRNA干扰组[（1119．55±158．60）mm3，P〈0．01]和生理盐水组[（1357．64±197．92）mm3，P〈0．01]。siRNA干扰联合HCPT治疗组小鼠的生存时间为26d，明显长于HCPT治疗组（14d，P〈0．05）、siRNA干扰组（21d，P〈0．05）和生理盐水组（12d，P〈0．05）。siRNA干扰联合HCPT治疗组移植瘤组织中H22细胞坏死明显，Bcl-2mRNA表达下调，s期细胞比例减少，凋亡率升高。结论与单一治疗组比较，Bcl-2siRNA与HCPT联合对小鼠H22肝癌移植瘤的生长有明显的抑制作用，并可延长小鼠的生存时间。

关键词： RNA干扰 Bcl-2基因 10-羟基喜树碱肝肿瘤小鼠

The Analgesic Effect of Total Flavonoids Extracted from Chaenomels lagenaria and its mechanical analysis

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The Analgesic Effect of Total Flavonoids Extracted from Chae...

覆膜条件下冬小麦根系生理特性及其空间分布变化

第三届中俄国际药理学研讨会

作者： Kong Jin-song Yang xing-hai Medical College of Three Gorges University,Yichang,Hubei 443002

AIM To evaluate the analgesic action and its mechanisms of total flavonoids from Chaenomels lagenaria (FLC).METHODS By the hot-plate and twisting body test in mouse and peripheral model of the potassium iontophoretic ...

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干旱地区农业研究 2006年第6期24卷 1-6页

作者：宋海星李生秀西北农林科技大学资源环境学院

覆膜条件下测定了冬小麦的根系活力与吸收面积,研究其空间分布情况。结果表明,在水平方向上,TTC还原量和TTC还原强度均随着离主茎距离的增加而减少;在垂直方向上,TTC还原量随着土层深度的加深而递减,TTC还原强度,返青期由上而下递减,开... 详细信息

覆膜条件下测定了冬小麦的根系活力与吸收面积,研究其空间分布情况。结果表明,在水平方向上,TTC还原量和TTC还原强度均随着离主茎距离的增加而减少;在垂直方向上,TTC还原量随着土层深度的加深而递减,TTC还原强度,返青期由上而下递减,开花期则上下土层无差异。根系总吸收面积和活跃吸收面积,在水平方向和垂直方向的变化与TTC还原量相同。覆膜处理并没有改变以上各指标的空间分布状态。但返青期水平方向的比吸收面积和比活跃吸收面积的变化趋势随覆盖地膜与否而不同,不覆膜处理由近到远递减,覆膜处理则无差异。返青期比吸收面积和比活跃吸收面积的垂直方向变化以及开花期二者的水平和垂直方向变化均无明显差异。根系活力与根系总吸收面积、活跃吸收面积呈极显著正相关。

关键词：覆膜冬小麦根系活力根系吸收面积空间分布

根系的吸收作用及土壤水分对硝态氮、铵态氮分布的影响

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中国农业科学 2005年第1期38卷 96-101页

作者：宋海星李生秀湖南农业大学资源与环境学院长沙410128 杨凌712100 西北农林科技大学资源与环境学院西北农林科技大学资源与环境学院杨凌712100

研究了玉米根系的吸收作用和土壤水分对耕层土壤硝态氮迁移及分布的影响。结果表明,根系发育状况及水分供应明显影响硝态氮的迁移及分布。根系自然生长和灌水处理,距主茎不同距离的各位点硝态氮浓度差异小;而限制根系生长和不灌水处理,... 详细信息

研究了玉米根系的吸收作用和土壤水分对耕层土壤硝态氮迁移及分布的影响。结果表明,根系发育状况及水分供应明显影响硝态氮的迁移及分布。根系自然生长和灌水处理,距主茎不同距离的各位点硝态氮浓度差异小;而限制根系生长和不灌水处理,则差异较大。距主茎0～10cm范围的耕层土壤中硝态氮含量的变化趋势是由远到近逐渐降低。这种变化趋势与耕层土壤中根系吸收面积的变化趋势相反。限制根系生长时,各位点土壤硝态氮浓度与土壤水分有相当一致的变化规律,说明随着植物吸水硝态氮作为溶质向根表迁移。与硝态氮不同,铵态氮的迁移和分布不受根系发育状况及水分供应的影响。

关键词：水分根系发育养分迁移硝态氮铵态氮

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分子生物学开放性实验的探索

实验室研究与探索 2009年第6期28卷 119-121页

作者：王丹宋海星何浪成都医学院基础医学院四川成都610081

为了探索实验教学新模式,培养创新型、应用型人才。从申请的学生中挑选5位06级的生物技术本科、1位06级临床专业的本科生。学生自查文献、自选方案、自行设计,并具体实施,最后指导教师根据学生提交的实验原始记录点评。通过对未知细菌16... 详细信息

为了探索实验教学新模式,培养创新型、应用型人才。从申请的学生中挑选5位06级的生物技术本科、1位06级临床专业的本科生。学生自查文献、自选方案、自行设计,并具体实施,最后指导教师根据学生提交的实验原始记录点评。通过对未知细菌16SrDNA的克隆等实验操作,学生基本掌握分子生物学的相关理论和实验技能。所以,开设开放性实验是提高分子生物学教学质量的有效途径。不仅可激发学生的学习兴趣,且可促使学生更善于发现问题、解决问题,显著提高了学生主动学习的能力与创新能力。

关键词：分子生物学开放性实验实验教学

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玉米根系活力及吸收面积的空间分布变化

西北农业学报 2005年第1期14卷 137-141页

作者：宋海星王学立湖南农业大学资源环境学院湖南长沙410128 陕西省生产力促进中心陕西西安710054

分别用氯化三苯基四氮唑 ( TTC)还原法和甲基蓝吸附法测定玉米根系活力和吸收面积 ,研究其 0～1 5 cm土层根系的水平分布和离主茎 0～ 2 0 cm范围土壤根系的垂直分布情况。结果表明 ,TTC还原量在离主茎 0～ 2 0 cm的水平方向和离地面 0... 详细信息

分别用氯化三苯基四氮唑 ( TTC)还原法和甲基蓝吸附法测定玉米根系活力和吸收面积 ,研究其 0～1 5 cm土层根系的水平分布和离主茎 0～ 2 0 cm范围土壤根系的垂直分布情况。结果表明 ,TTC还原量在离主茎 0～ 2 0 cm的水平方向和离地面 0～ 45 cm的垂直方向上 ,随着离主茎距离的增加和土层深度的加深而降低。TTC还原强度在水平方向上与 TTC还原量的变化趋势类似 ,只是个别生育期 1 5～ 2 0 cm处回升 ;在垂直方向上与 TTC还原量的变化趋势相反 ,随土层深度的加深而增加。同样范围内根系总吸收面积和活跃吸收面积的变化趋势与 TTC还原量相同 ;而比吸收面积和比活跃吸收面积 ,在水平方向上随着离主茎距离的增加而明显增加。

关键词：玉米根系活力根系吸收面积空间分布氯化三苯基四氮唑还原法甲基蓝吸附法