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Impact of Plant Density on the Formation of Potato Mimitubers Derived from Microtubers and Tip-Cuttings in Plastic Houses

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Journal of Integrative Agriculture 2013年第6期12卷 1008-1017页

作者： JIN Hui LIU Jun song bo-tao XIE Cong-hua Key Laboratory of Horticultural Plant Biology Ministry of Education/Potato Engineering Research Center of Hubei Province Huazhong Agricultural University

The potato minitubers have been widely used for the elite seed propagation to improve the seed potato system in China. However, little information is available for an efficient production of the minitubers with high plant density in the protected growing conditions like plastic houses. In present research, the minitubers of a wide-grown potato variety, Favorita, were produced with the microtubers from tissue culture and the tip-cuttings of the microtuber plants. Three plant densities, 200, 400 and 600 microtubers or plants m-2 were set up with the randomized block design of 3 replications and the experiment was repeated in 4 seasons in 2009-2010. The canopy development, light interception, dry weight production and partitioning, tuberization and tuber weight were investigated to elucidate the mechanism by which the plant density affects the formation and growth of the minitubers. The results showed that the number of the tubers formed per unite area was in line with the increase in plant density. The difference in leaf area index （LAI） between the plant densities, especially in early stage of the plant growth, resulted in more radiation interception and dry weight producing in higher plant density than in lower one. However, our analysis demonstrated that the conversion coefficient of the cumulative intercepted radiation to plant weight and the dry weight partition rate to the tubers were constant between plant densities, suggesting that less amount of the photoassimilates partitioned to individual tubers is causal for more small tubers in high plant density. A negative exponential curve model, determined by total number of tubers produced per unit area and the mean tuber weight, fitted well to the tuber size distribution pattern. The optimum plant density could be estimated from this model for a maximum production of the minitubers with desired size.

关键词： potato minituber microtuber tip-cutting plant density

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Population Improvement of Resistance to Late Blight in Tetraploid Potato:A Case Study in Combination with AFLP Marker Assisted Background Selection

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Agricultural Sciences in China 2011年第8期10卷 1177-1187页

作者： YAO Chun-guang song bo-tao LIU Jun WU Cheng-jin CHENG Qun LI Da-chun XIE Cong-hua Key Laboratory of Horticultural Plant Biology Ministry of Education/National Center for Vegetable Improvement （Central China）/Potato Engineering and Technology Research Center of Hubei Province/Huazhong Agricultural UniversityWuhan 430070P.R.China Southern Potato Research Center of China/Enshi Academy of Agricultural Sciences Enshi 445000P.R.China

Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potato *** recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1 population without R1-R11 dominant genes has been released by the International Potato Center at the short-day condition of *** present research was carried out to further improve the resistance and the agronomic traits of B3C1 population under long-day condition of Hubei,China,with maximized retention of its genetic ***-seven individual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aiming to elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day *** late blight resistance and the main agronomic traits including the maturity,the plant characters and the tuber traits were evaluated for the foreground selection in three years,by which 130 pedigrees were maintained as the basic population of B3C2 for further selection.A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used to monitor the genetic diversity of the populations for the background *** B3C2 population of 51 clones was finally selected,of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity was maintained by about 96% as referred to *** results strongly suggested that combination of the foreground selection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrent selection of tetraploid potato to improve quantitative traits.

关键词： potato recurrent selection late blight horizontal resistance genetic diversity

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Variability and reduction of atmospheric pollutants in Beijing and its surrounding area during the Beijing 2008 Olympic Games

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Chinese Science Bulletin 2010年第18期55卷 1937-1944页

作者： XIN JinYuan WANG YueSi TANG GuiQian WANG LiLi SUN Yang WANG YingHong HU bo song tao JI DongSheng WANG WeiFeng LI Liang LIU GuangRen State Key Laboratory of Atmospheric boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric Physics Chinese Academy ofSciences Beijing 100029 China

The Beijing-Tianjin-Hebei Atmospheric Environment Monitoring Network was established by the Institute of Atmospheric Physics,Chinese Academy of *** goals of the network were to monitor and provide warnings of the atmospheric quality in Beijing and its surrounding area during the Beijing 2008 Olympic *** results showed that the atmospheric complex pollution exhibited high concentrations of ozone and fine particles and oxidation in summer,with a ubiquitous regional *** regional mean concentrations of SO2,PM2.5,NO2,and O3_8h max(the maximum daily 8 h mean) and Ox were 22±11,90±40,25±5,136±35 and 112±21 μg/m3 in summer,*** the Olympic Games,the mean concentration of SO2,PM2.5,NO2,O3_8h max,and Ox were 12.5±4,56±28,23±4,114±29,95±17 μg/m3 in the region,respectively,and fell by 51.0%,43.7%,13%,20.2%,and 18.9%,respectively,compared to the prophase mean before the Olympic *** concentration of atmospheric pollutants declined significantly and achieved the "Green Olympics" control goal of air *** the Olympic Games,SO2,PM2.5 and NOx increased significantly as the temporary atmospheric pollution control measures were terminated.

关键词：北京2008年奥运会大气污染物周边地区 2008年北京奥运会环境监测网络 PM2.5 平均浓度二氧化硫

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马铃薯sAGP基因表达对块茎淀粉和还原糖含量的影响

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中国农业科学 2005年第7期38卷 1439-1446页

作者：宋波涛谢从华柳俊华中农业大学/国家蔬菜改良中心华中分中心/园艺植物生物学教育部重点实验室/湖北省马铃薯工程技术研究中心华中农业大学/国家蔬菜改良中心华中分中心/园艺植物生物学教育部重点实验室/湖北省马铃薯工程技术研究中心武汉430070

通过根癌农杆菌(Agrobacteriumtumefaciens)介导将CaMV35S驱动的正/反义sAGP基因导入马铃薯(SolanumtuberosumL.)品种鄂马铃薯3号(E3)和南中552(N552)以研究sAGP基因对马铃薯块茎淀粉-糖代谢的影响。PCR扩增和Southern杂交证明正/反义sAGP基因已整合到马铃薯基因组中。Northern杂交分析表明,该正/反义基因在转基因植株中均能正常转录。转基因植株块茎分析显示,导入正义基因的株系中除4个与转反义基因表现相同外,其余19个株系AGPase酶活性平均上升约25%,还原糖含量下降20%左右,淀粉含量增加5%～6%。其中3个测定指标与各自对照相比,差异均达到显著水平的有A8、C1和C5,最大变化幅度分别为酶活性上升53.5%,还原糖含量下降60.1%,淀粉含量增加33.9%。转反义sAGP基因的15个株系中,AGPase酶活性平均下降约27%,还原糖含量上升近36%,淀粉含量下降亦达27%,其中株系B5、B6、B8和D7的这3个指标均与各自未转基因对照差异显著。本研究结果表明,sAGP基因与AGPase酶活性直接相关,而AGPase很大程度上调节着马铃薯块茎淀粉-糖代谢,通过增强sAGP表达,能有效改良块茎的加工品质。

关键词：马铃薯 sAGP 基因表达淀粉还原糖

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翼吊发动机吊架结构等效建模及减振特性分析

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科学技术与工程 2012年第4期20卷 832-835页

作者：宋波涛贺尔铭张钊西北工业大学航空学院西安710072

为了验证翼吊发动机吊架结构对发动机振动传递的减振效果,必须对其进行相关的结构动力学分析。根据实际发动机吊架结构,采用ANSYS软件建立了吊架结构的等效模型,并对其进行了振动模态分析。在发动机三种典型工况(起飞、巡航、空慢)的实... 详细信息

为了验证翼吊发动机吊架结构对发动机振动传递的减振效果,必须对其进行相关的结构动力学分析。根据实际发动机吊架结构,采用ANSYS软件建立了吊架结构的等效模型,并对其进行了振动模态分析。在发动机三种典型工况(起飞、巡航、空慢)的实测载荷作用下,分析了吊架结构的减振特性,并研究了吊架结构减振效果与结构动刚度的关系。研究结果表明,发动机吊架结构设计具有较好的减振效果,但由于吊架结构的动刚度随激振力频率变大而增大,其高频减振效果稍有降低。

关键词：发动机吊架 ANSYS 振动传递模态分析动刚度

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噪声中正(余)弦信号相位差的检测

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电测与仪表 2006年第11期43卷 10-11页

作者：李泽光宋伯涛大连大学信息工程学院辽宁大连116622

若两路正(余)弦被测信号含有加性噪声,要准确测量其相位差必须要采取一定措施消除噪声的影响。用正交双通道处理技术对此两信号进行互相关函数处理,可以检测出被噪声淹没的正(余)弦信号的相位差。

关键词：互相关函数零中频处理相位差

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Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model

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Neural Regeneration Research 2025年第3期20卷 858-872页

作者： Xintian Ding Chun Chen Heng Zhao Bin Dai Lei Ye tao song Shuai Huang Jia Wang tao You Department of Orthopedics The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Orthopedics Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhui ProvinceChina

Reducing the secondary inflammatory response, which is partly mediated by microglia, is a key focus in the treatment of spinal cord injury. Src homology 2-containing protein tyrosine phosphatase 2(SHP2), encoded by PTPN11, is widely expressed in the human body and plays a role in inflammation through various mechanisms. Therefore, SHP2 is considered a potential target for the treatment of inflammation-related diseases. However, its role in secondary inflammation after spinal cord injury remains unclear. In this study, SHP2 was found to be abundantly expressed in microglia at the site of spinal cord injury. Inhibition of SHP2 expression using siRNA and SHP2 inhibitors attenuated the microglial inflammatory response in an in vitro lipopolysaccharide-induced model of inflammation. Notably, after treatment with SHP2 inhibitors, mice with spinal cord injury exhibited significantly improved hind limb locomotor function and reduced residual urine volume in the bladder. Subsequent in vitro experiments showed that, in microglia stimulated with lipopolysaccharide, inhibiting SHP2 expression promoted M2 polarization and inhibited M1 polarization. Finally, a co-culture experiment was conducted to assess the effect of microglia treated with SHP2 inhibitors on neuronal cells. The results demonstrated that inflammatory factors produced by microglia promoted neuronal apoptosis, while inhibiting SHP2 expression mitigated these effects. Collectively, our findings suggest that SHP2 enhances secondary inflammation and neuronal damage subsequent to spinal cord injury by modulating microglial phenotype. Therefore, inhibiting SHP2 alleviates the inflammatory response in mice with spinal cord injury and promotes functional recovery postinjury.

关键词： apoptosis glycolysis inflammatory response microglia neurons polarization spinal cord injury Src homology 2-containing protein tyrosine phosphatase 2

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SSR标记的彩色马铃薯遗传多样性分析及指纹图谱构建

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农业生物技术学报 2012年第4期20卷 362-371页

作者：石景宋波涛金开建柳俊华中农业大学园艺植物生物学教育部重点实验室湖北省马铃薯工程技术研究中心

彩色马铃薯(指块茎的皮或肉为红、蓝、紫、橙色等)近年来日益为育种工作者所关注,很多彩色马铃薯品种(系)从形态学上难以鉴定是否为同一基因型,给育种工作带来诸多不便。本研究利用SSR标记对50份彩色马铃薯(Solanum tuberosumL.)材料进... 详细信息

彩色马铃薯(指块茎的皮或肉为红、蓝、紫、橙色等)近年来日益为育种工作者所关注,很多彩色马铃薯品种(系)从形态学上难以鉴定是否为同一基因型,给育种工作带来诸多不便。本研究利用SSR标记对50份彩色马铃薯(Solanum tuberosumL.)材料进行了遗传多样性分析及指纹图谱构建。研究筛选出56对马铃薯SSR引物,对50份材料的基因组DNA进行PCR扩增,共检测出236个等位位点,其中多态性位点230个,多态性比率达97.46%。分析显示,基因型间遗传相似性系数在0.50~1.00之间。UPGMA聚类分析表明,在相似系数0.63处可将全部材料分为3大类。利用5对核心引物构建了50份供试材料的指纹图谱,并证明其属于44个基因型的,为彩色马铃薯资源鉴定和利用提供了依据。

关键词：彩色马铃薯 SSR标记聚类分析指纹图谱遗传多样性

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马铃薯腺苷二磷酸葡萄糖焦磷酸化酶小亚基基因(sAGP)的克隆及其在毕赤酵母中的表达

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农业生物技术学报 2005年第3期13卷 282-287页

作者：宋波涛柳俊谢从华成善汉华中农业大学园艺林学学院国家蔬菜改良中心华中分中心园艺植物生物学教育部重点实验室湖北省马铃薯工程技术研究中心湖北省马铃薯工程技术研究中心武汉430070

用RT-PCR结合RACE的方法,在腺苷二磷酸葡萄糖焦磷酸化酶小亚基基因(sAGP)的保守位点设计1对引物,从低温处理的马铃薯(SolunumtaberosusL.)块茎cDNA中,扩增出1条约1.9kb的特异性条带,并将其克隆到pBluescriptSKⅡ载体。DNA序列测定结果表... 详细信息

用RT-PCR结合RACE的方法,在腺苷二磷酸葡萄糖焦磷酸化酶小亚基基因(sAGP)的保守位点设计1对引物,从低温处理的马铃薯(SolunumtaberosusL.)块茎cDNA中,扩增出1条约1.9kb的特异性条带,并将其克隆到pBluescriptSKⅡ载体。DNA序列测定结果表明,目标片段为sAGP,全长为1854bp,包含一个1563bp的开放阅读框架,编码521个氨基酸。5’端有63bp的非翻译区,3’端包含219bp的非编码序列,3’端poly(A)尾端上游12和19bp处有加尾信号AATAAA。该基因已在GenBank注册,登记号为AY186620。将该基因PCR突变后,克隆到酵母表达载体pMETα-B中,构成表达载体pMETαA4,将其线性化,用电穿孔法导入Pichia-methabolicapMAD16,得到重组表达酵母,经甲醇诱导表达后,SDS-PAGE电泳显示,在诱导表达重组酵母中检测到50kD左右的特异蛋白带。裂解酵母菌体提取蛋白质,进行酶活力活性测定,结果表明诱导表达重组酵母中AGPase活性明显上升,证明诱导表达成功。

关键词：马铃薯sAGP克隆酵母表达酶活性

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烟草液泡转化酶抑制子基因调控马铃薯块茎低温还原糖累积的研究

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中国农业科学 2007年第1期40卷 140-146页

作者：成善汉宋波涛谢从华李竟才柳俊国家蔬菜改良中心华中分中心/园艺植物生物学教育部重点实验室/湖北省马铃薯工程技术研究中心华中农业大学园艺林学学院武汉430070

【目的】克隆烟草液泡转化酶抑制子基因,并在马铃薯中表达,从而抑制低温贮藏块茎还原糖积累,提高马铃薯加工品质。【方法】通过RT-PCR法分离Nt-VIF全长cDNA,转化马铃薯植株,PCR、Northern杂交和Southern杂交检测转基因植株,转基因植株... 详细信息

【目的】克隆烟草液泡转化酶抑制子基因,并在马铃薯中表达,从而抑制低温贮藏块茎还原糖积累,提高马铃薯加工品质。【方法】通过RT-PCR法分离Nt-VIF全长cDNA,转化马铃薯植株,PCR、Northern杂交和Southern杂交检测转基因植株,转基因植株块茎在低温和常温条件下贮藏30d,分析转化酶活性与还原糖含量。【结果】克隆到Nt-VIF全长cDNA,35S调控下正向的Nt-VIF基因cDNA已经成功转入鄂马铃薯3号(E3)植株。转基因植株块茎在4℃和20℃贮藏30d表明,VI活性受温度调节,高温贮藏块茎的VI活性显著低于低温贮藏的块茎,且高温条件下转基因块茎与对照无显著差异,而低温条件下转基因块茎的VI活性与对照相比下降幅度为22.7%(株系A-3)～78.1%(株系A-31)。转基因块茎还原糖(RS)含量在高温贮藏条件下与对照差异亦不明显,而低温贮藏条件下RS含量下降幅度为20%(株系A-17)～80.5%(株系A-30)。进一步分析还表明,转基因块茎低温贮藏其液泡转化酶活性与还原糖含量呈显著正直线相关(RS=0.289VI+0.0736)。【结论】Nt-VIF基因的表达显著抑制了马铃薯液泡转化酶活性,导致还原糖积累降低。

关键词：马铃薯低温糖化转化酶抑制子遗传转化

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