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Synthesis and Characterization of <i>Citrus limonum</i>Essential Oil Based Nanoemulsion and Its Enhanced Antioxidant Activity with Stability for Transdermal Application

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Journal of Biomaterials and Nanobiotechnology 2020年第4期11卷 215-236页

作者： Harleen Kaur Pranav Pancham Ramneek Kaur Shriya Agarwal manisha singh Amity Institute of Biotechnology Amity University Noida U.P. India Department of Biotechnology Jaypee Institute of Information Technology (JIIT) Noida U.P. India School of Medicine Western Sydney University Campbelltown New South Wales Australia

Lemon oil (LO), also known as Citrus limonum is a highly volatile essential oil (EO) with potential therapeutic properties like anti-oxidative, anti-proliferative, anti-fungal and anti-cancerous. However, the efficacy of LO is limited due to its physiological factors such as high volatility, poor stability (particularly sensitive to sunlight) and quick degradability upon exposure. To overcome these challenges, we formulated lemon oil loaded nanoemulsion system (LO-NE) (oil-in-water), using aqueous titration method. The formulation comprised of lemon oil (LO), Tween 80 and ethanol as oil, surfactant and co-surfactant phases respectively. The existence zone of NE was established by constructing pseudo-ternary phase diagrams using different concentrations of LO, surfactant and co-surfactant (Smix). The quantitative estimation of LO was performed using a high throughput gas chromatography, revealing the presence of various compounds like Limonene, Alpha-Pinene and Linalyl acetate followed by the estimation of total phenolics and flavonoid content. The characterization of LO-NE indicated the particle size of 60 ± 2.5 nm along with the polydispersity index of 0.125 and zeta potential of −14.9 mV. The size range of the NE particles dispersed in the colloidal system was further verified by TEM micrograph which shows size range between 46.2 - 104.7 nm. All the anti-oxidant assays outcomes exhibited the higher activity of LO-NE in comparison to LO alone with lower IC50 values. The release kinetics statistical data showed that LO-NE had a sustained release and followed the Higuchi’s model in comparison to burst release of LO alone. Lastly, the stability analysis of the optimised formulation (LO-NE) and LO was estimated through antioxidant assay and subjecting them for thermodynamic stability after 6 months. The results attained, showed higher stability and anti-oxidant capability of LO-NE than LO alo

关键词： Lemon Oil Surfactants Particle Size Analysis Ternary Phase Diagrams Release Kinetics Thermodynamic Stability

Controllable Transdermal Drug Delivery of Theobroma cacao Extract Based Polymeric Hydrogel against Dermal Microbial and Oxidative Damage

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Food and Nutrition Sciences 2019年第10期10卷 1212-1235页

作者： Shriya Agarwal Vandana Tyagi Mugdha Agarwal Arushi Pant Harleen Kaur Rachana manisha singh Department of Biotechnology Jaypee Institute of Information Technology Noida India Amity Institute of Biotechnology Amity University Noida India.

Human dermal layers are directly bared to the external environmental adversities like pollution, radiation, dust along with various chemical and mechanical stress conditions which constantly lead to the oxidative stress, eventually forming free radicals. These conditions also support the dermal microbial infections by invading the cutaneous layers. Therefore, the most efficient approach accepted globally to combat these complications is to opt for transdermal application of exogenous antioxidants which helps in reducing the ill effects of oxidative stress and promotes the DNA repair. However, many scientific findings exhibited the potential role of Theobroma cacao for providing efficient skin protection. Theobroma cacao known for its vital procyanidin flavonoids, phenolic compounds, methylxanthines, catechin and epicatechin phytoconstituents with numerous health benefits, besides helping in smoothening and softening the damaged skin. These therapeutic benefits of cocoa phytocompounds are attributed to its anti-inflammatory and antioxidative characteristics. This study addresses the fabrication and optimizations of Theobroma cacao extract loaded carbopol hydrogel system for increased antioxidative and antimicrobial effects. It was found in the studies that the hydrogel-based T. cacao extract (TCHG) has significantly improved the therapeutic index of the extract, making it more suitable for transdermal application. The optimized hydrogel was further characterized by physicochemical parameters, SEM analysis and in-vitro release kinetics. The reported findings showed the sustained compound release with comparatively significant antimicrobial activity of TCHG against microbial (Pseudomonas fluorescens, Bacillus licheniformis, Micrococcus luteus, Aspergillus niger, Trichoderma harzianum, Rhizopus oryzae) infections. Also, the higher ROS quenching ability of TCHG as compared to T. cacao extract promises to be an effective transdermal formulation.

关键词： Theobromine Hydrogel Oxidative Stress Release Kinetics Transdermal

Analyzing the Prebiotic Potential of Glucosamine for Targeting the Gut Microbiome Health

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Food and Nutrition Sciences 2023年第2期14卷 119-134页

作者： Pranav Pancham Divya Jindal Archita Gupta Deepshikha Yadav Shriya Agarwal Saurabh Jha manisha singh Department of Biotechnology Jaypee Institute of Information Technology (JIIT) Noida India Neuroimmunology Laboratory School of Medicine Western Sydney University Campbelltown Australia Birla Institute of Technology Mesra India Indian Institute of Technology-Kanpur Kanpur India Bhartiya Nirdeshak Dravya Division CSIR-National Physical Laboratory New Delhi India Physico-Mechanical Metrology Division CSIR-National Physical Laboratory New Delhi India Academy of Scientific and Innovation Research (AcSIR) Ghaziabad India School of Natural Sciences Macquarie University Sydney Australia Department of Biotechnology School of Engineering and Technology Sharda University Greater Noida India

Recognizing the composition and modulation of the microbiome, a viable therapeutic tool for multi-targeted therapy is a new strategy that has recently been explored. Glucosamine (GS) is being studied for its prebiotic potential in addition to being the most abundant and naturally occurring amino monosaccharide. The current study focuses on glucosamine’s prebiotic potential by assessing the stability of various GS concentrations (1% - 5%) in the gastrointestinal tract (GIT) and its ability to be fermented by the gut microbiota. The results showed that GS stimulated the most growth in L. acidophilus even after a longer incubation time than B. bifidum and L. acidophilus growth was concentration-dependent, with maximum growth at 3% with a simultaneous decrease in pH (5.6 - 1.7). The decrease in GS concentration with time also represented the growth of bacterial species, demonstrating the species’ utilization of GS. Furthermore, at 3%, GS also represented the prebiotic index of 1.9. In addition, the concentration of GS in various simulated GIT fluids was estimated in both fast and fed conditions to examine GS stability at various levels in the gut. The results showed that GS remained unaffected and non-digestible in all of the simulated GIT fluids (salivary, gastric, intestinal, and colonic), but there was a slight decrease in GS concentration (2.8%) in the fasted state of gastric fluid due to low pH levels (1.6). As a result, the findings are conclusive and suggest that GS possesses prebiotic properties.

关键词： Microbiome Enteric Nervous System (ENS) Prebiotic Index Hexosamine Biosynthetic Pathway (HBP) Vagal Afferents Phosphotransferase System

<i>In-Silico</i>Validation and Development of Chlorogenic Acid (CGA) Loaded Polymeric Nanoparticle for Targeting Neurodegenerative Disorders

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Journal of Biomaterials and Nanobiotechnology 2020年第4期11卷 279-303页

作者： Vinayak Agarwal Shriya Agarwal Ramneek Kaur Pranav Pancham Harleen Kaur Siddhi Bhardwaj manisha singh Department of Biotechnology Jaypee Institute of Information Technology (JIIT) Noida U.P. India School of Medicine Western Sydney University Campbelltown New South Wales Australia Amity Institute of Biotechnology Amity University Noida U.P. India

Background: Recent decades witnessed a significant growth in terms of phytocompounds based therapeutics, extensively explored for almost all types of existing disorders. They have also been widely investigated in Neurodegenerative disorders (NDDs) and Chlorogenic acid (CGA), a polyphenolic compound having potential anti-inflammatory and anti-oxidative properties, emerged as a promising compound in ameliorating NDDs. Owing to its poor stability, bioavailability and release kinetics, CGA needed a suitable nanocarrier based pharmaceutical design for targeting NDDs. Objective: The current study is aimed at the in-silico validation of CGA as an effective therapeutic agent targeting various NDDs followed by the fabrication of polymeric nanoparticles-based carrier system to overcome its pharmacological limitations and improve its stability. Methods: A successful in-silico validation using molecular docking techniques along with synthesis of CGA loaded polymeric nanoparticles (CGA-NPs) by ionic gelation method was performed. The statistical optimisation of the developed CGA-NPs was done by Box Behnken method and then the optimized formulation of CGA-NPs was characterised using particle size analysis (PSA), Transmission electron microscopy (TEM), Fourier Transform Infrared spectroscopy (FTIR) along with in-vitro release kinetics analysis. Results & Conclusion: The results attained exhibited average particle size of 101.9 ± 1.5 nm, Polydispersibility (PDI) score of 0.065 and a ZP of −17.4 mV. On a similar note, TEM results showed a size range of CGA-NPs between 90 - 110 nm with a spherical shape of NPs. Also, the data from in-vitro release kinetics showed a sustained release of CGA from the NPs following the first-order kinetics suggesting the appropriate designing of nanoformulation.

关键词： Antioxidant Anti-Inflammatory Polymeric Nanoparticles Release Kinetics Box Behnken Design Molecular Docking Particle Size Analysis

Synthesis and Characterization of Citrus limonum Essential Oil Based Nanoemulsion and Its Enhanced Antioxidant Activity with Stability for Transdermal Application

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Analyzing the Prebiotic Potential of Glucosamine for Targeting the Gut Microbiome Health

Journal of Biomaterials and Nanobiotechnology 2020年第4期

作者： Harleen Kaur Pranav Pancham Ramneek Kaur Shriya Agarwal manisha singh

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Review of Electrochemical Sensors and Biosensors Based on First-Row Transition Metals,Their Oxides,and Noble Metals Nanoparticles

Food and Nutrition Sciences 2023年第2期

作者： Pranav Pancham Divya Jindal Archita Gupta Deepshikha Yadav Shriya Agarwal Saurabh Jha manisha singh

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Journal of Analysis and Testing 2024年第2期8卷 143-159页

作者： Kulveer singh Kuldeep Kumar Maurya manisha Malviya Department of Chemistry Indian Institute of Technology(Banaras Hindu University)Varanasi221005India

The use of electrochemical sensors for sensitive disease diagnosis and detecting various species with pharmacological,therapeutic,industrial,food-related,and environmental origins is now widely accepted.A catalytic or binding event resulting from the sensor’s electroactive component recognizing its analyte creates an electrical signal proportionate to the analyte concentration,which is then monitored by a *** development of morphologically distinct metal and metal oxide nanoparticles formed from first-row transition elements(Mn,Cr,Fe,Co,Ti,Ni,Cu,Zn)and noble metals(Pt,Au,Ag,Pd)is described in this *** effect of these metal nanoparticles has been studied using Tetracyanoquinodimethane(TCNQ),Ferrocene,and other organic compounds as electroactive species using carbon paste-modified *** sensors,mostly based on ferrocene,are exceedingly sensitive,selective,affordable,and for detecting numerous biomolecules like glucose,dopamine,NADH,ascorbic acid,and a few dyes and are simple to *** recent decades,charge transfer organic species-based chemosensors have become a prominent study *** paper outlines current developments in electrochemical biosensors based on transition metal nanoparticles,covering glucose,ascorbic acid,uric acid,and other inorganic and organic *** importance of transition metal and transition metal oxide nanoparticles as potential electrode modifiers for developing sensors is highlighted.A discussion of the present problem and possible solutions,and plausible future directions marks the review’s conclusion.

关键词： Electrochemical sensing Amperometry Cyclic voltammetry Square wave voltammetry Linear sweep voltammetry

Variation in cone and seed characters in blue pine (Pinus wallichiana) across natural distribution in western Himalayas

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Journal of Forestry Research 2012年第2期23卷 235-239页

作者： Ombir singh manisha Thapliyal Forest Tree Seed Laboratory Silviculture DivisionForest Research InstituteDehradun 248006India

We analyzed 17 seed sources （seed stands） ofPinus wallichiana for variations present in cone and seed characters, scattered over natural distribution in north-west Himalayan states （Uttarakhand and Himachal Pradesh） of India. The significant variations were observed in cone weight, cone length, cone width, seed length, seed width, seed weight, seed germination, radicle length, and plumule length among different seed sources of the species. Significant positive correlation between seed weight, cone weight and cone width showed that seed weight in the species depend more on the cone size. Seed germination was also positively correlated with seed weight, cone weight and radicle length in the study. The estimates of variability with regard to genetic parameters for seed weight, seed germination, cone length, cone width, cone weight showed wide range of variation in the study. Seed weight showed high heritability values coupled with maximum genetic gain. Traits with such values indicate presence of good amount of heritable additive components and are under strong genetic control. The findings of the study revealed that seed sources expressed both phenotypic and genotypic differences in the seed and cone traits which might be due to the differences in genetic make up of various seed sources and environmental factors i,e. genotypic and environmental interaction, The study suggests that the seed weight should be given the top priority for the further improvement of this species.

关键词： seed sources seed traits heritability genetic gain improve-ment.

Gate leakage current reduction in IP3 SRAM cells at 45 nm CMOS technology for multimedia applications

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Journal of Semiconductors 2012年第5期33卷 88-92页

作者： R.K.singh Neeraj Kr.Shukla manisha Pattanaik Bipin Tripathi Kumaon Institute of Technology DwarahatAlmora(Uttarakhand)India ITM University Department of EECEGurgaon(Haryana)India ABV-IIITM VLSI GroupDepartment of ITGwalior(Madhya Pradesh)India

We have presented an analysis of the gate leakage current of the IP3 static random access memory （SRAM） cell structure when the cell is in idle mode（performs no data read/write operations） and active mode （performs data read/write operations）,along with the requirements for the overall standby leakage power,active write and read powers.A comparison has been drawn with existing SRAM cell structures,the conventional 6T,PP, P4 and P3 *** the supply voltage,V_（DD） = 0.8 V,a reduction of 98%,99%,92%and 94%is observed in the gate leakage current in comparison with the 6T,PP,P4 and P3 SRAM cells,respectively,while at V_（DD） = 0.7 V,it is 97%,98%,87%and 84%.A significant reduction is also observed in the overall standby leakage power by 56%〉, the active write power by 44%and the active read power by 99%,compared with the conventional 6T SRAM cell at V_（DD）= 0.8 V,with no loss in cell stability and performance with a small area *** simulation environment used for this work is 45 nm deep sub-micron complementary metal oxide semiconductor（CMOS） technology,t_（ox） = 2.4 nm,K_（thn） = 0.22 V,K_（thp） = 0.224 V,V_（DD） = 0.7 V and 0.8 V,at T = 300 K.

关键词： gate leakage subthreshold leakage low power deep sub-micron SRAM