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Insect Science 2020年第5期27卷 947-963页

作者： Xiao-Li Bing Dian-shu zhao Chang-Wu Peng Hai-Jian Huang Xiao-Yue Hong Department of Entomology Nanjing Agricultural UniversityNanjingChina

Rice planthoppers are notorious plant sap-feeding pests which cause serious *** several microbes in rice planthoppers have been broadly characterized,the abundance and diversity of bacteria and fungi in field planthoppers are largely *** study investigated the bacterial and fungal community compositions of Chinese wild rice planthoppers Laodelphax striatellus and Sogatella furcifera using parallel 16S rRNA gene amplicon and internal transcribed space region *** bacteria varied sig-nificantly between the species and were partitioned significantly by sex,tissues and host environments in each *** majority of bacteria were affiliated with the genera Wol-bachia,Cardinium,Rickettsia and *** abundance of Wolbachia was negatively correlated with that of Cardinium in both planthopper *** with bacteria,the abundance and diversity of fiungi did not differ between sexes but both were enriched in the *** bacterial community as a whole showed no significant correlation with the fungal *** majority of fungi were related to Sarocldium,Alternaria,Malasseria,Aspergillus and Curvularia.A phylogenetic analysis revealed that these fungi were closely related to botanic symbionts or *** results provide novel insights into the bacteria and fungi of rice planthoppers.

关键词： fungal diversity Laodelphax striatellus microbiota Sogatella furcifera planthoppers

The microbiota in spider mite feces potentially reflects intestinal bacterial communities in the host

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Insect Science 2020年第5期27卷 859-868页

作者： Yu-Xi Zhu Zhang-Rong Song Yue-Ling Song Dian-shu zhao Xiao-Yue Hong Department of Entomology Nanjing Agricultural UniversityNanjingChina

Microorganisms prov ide many physiological functions to herbivorous *** mites(genus Tetranychus)are important agricultural pests throughout the world;however,the composition of the spider mite microbial community,especially gut micro-biome,remains ***,we investigated the bacterial community in five spider mite species and their associated feces by deep sequencing of the 16S rRNA *** composi-tion of the bacterial community was significantly different among the five prevalent spider mite species,and some bacterial symbionts showed host-species ***,the abundance of the bacterial community in spider mite feces was significantly higher than that in the corresponding spider mite ***,Flavobacterium was detected in all samples,and represent a"core microbiome".Remarkably,the maternally inherited endosymbiont Wolbachia was detected in both spider mite and ***,these results offer insight into the complex community of symbionts in spider mites,and give a new direction for future studies.

关键词： bacterial community fces 16S rRNA spider mite Wolbachia

Raising medium function of environmental engineering by using life rubbish to produce carpet turf

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Journal of Environmental Sciences 2000年第4期12卷 498-505页

作者： DUO Li an, zhao shu lan (College of Chemistry and Biology, Tianjin Normal University, Tianjin 300074, China) 天津师范大学天津 3 0 0 0 74

Abundant material with low cost was utilized to raise medium function of producing carpet turf by life rubbish. The results showed that the material used could strikingly raise water retentiveness and ventilating of medium. Through the determination of the effect of each growth index of several turfgrass, emergence density, plant height, root growth and individual plant′s net primary production were all positively related to the material′s amount mixed, showing that the mixture of material could promote turfgrass growth, further proving that the material used raised medium function of producing carpet turf by life rubbish and made most use of life rubbish resources. It provided a scientific base is for application of environmental engineering by using life rubbish to produce carpet turf. So the study had both important theoretic meaning and applied value.

关键词： life rubbish carpet turf medium function environmental engineering

Two Divergent Members of 4-Coumarate： Coenzyme A Ligase from Salvia miltiorrhiza Bunge： cDNA Cloning and Functional Study

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Journal of Integrative Plant Biology 2006年第11期48卷 1355-1364页

作者： shu-Juan zhao Zhi-Bi Hu DI Liu Frederick C. C. Leungt Institute of Traditional Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Shanghai 201203 China Department of Zoology University of Hong Kong Hong Kong SAR China

4-Coumarate ： coenzyme A Ilgase （4CL） Is one of the key enzymes In phenylpropanoid metabolism leading to series of phenollcs, Including water-soluble phenolic acids, which are important compounds determining the medicinal quality of Danshen （Salvia miltiorrhiza Bunge）, a traditional Chinese medicinal herb. To Investigate the function of 4CL in the biosynthesis of water-soluble phenolic acid in Danshen, we have cloned two cDNAs （Sm4CL1 and Sm4CL2） encoding divergent 4CL members by applying nested reverse transcrlptlon-polymerase chain reaction （RT-PCR） with degenerate primers followed by 5′/3′rapid amplification of cDNA ends （RACE）（Note, these sequence data have been submitted to the GenBank database under accession numbers AY237163 and AY237164）. Either of the coding regions was inserted into a pRSET vector and a kinetic assay was performed with purified recombinant proteins. The substrate utilization profile of Sm4CL1 was distinct from that of Sm4CL2. The Km values of Sm4CL1 and Sm4CL2 to 4-coumarlc acid were （72.20±4.10） and （6.50±1.45） μmol/L, respectively. These results, In conjunction with Northern blotting and other information, imply that Sm4CL2 may play an Important role in the biosynthesis of watersoluble phenolic compounds, whereas Sm4CL1 may play a minor role in the pathway. Southern blotting analysis suggested that both Sm4CL1 and Sm4CL2 genes are present as a single copy and are located at different sites In the genome.

关键词： cDNA cloning 4-coumarate : coenzyme A ligase (4CL) Danshen (Salvia miltiorrhiza) enzymatic kinetic assays water-soluble phenolic acids.

Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo

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World Journal of Gastroenterology 2008年第12期14卷 1858-1865页

作者： Qing-Ling Wang Hui Wang shu-Li zhao Ya-Hong Huang Ya-Yi Hou Immunology and Reproductive Biology Lab Medical School and State Key Laboratory of PharmaceuticalBiotechnology Jiangsu Key Laboratory of Molecular MedicineNanjing University Nanjing 210093 Jiangsu Province China

AIM： To determine whether midkine （M/O and its truncated form （tMK） contribute to gastric tumorigenesis using in vitro and in vivo models. METHODS： Human MK and tMt（ plasmids were constructed and expressed in BGC823 （a gastric adenocarcinoma cell line） to investigate the effect of over-expressed MK or tMK on cell growth and turmorigenesis in nude mice. RESULTS： The growth of MK-transfected or tMK- transfected cells was significantly increased compared with that of the control cells, and tMK-transfected cells grew more rapidly than MK-transfected cells. The number of colony formation of the cells transfected with MK or tMK gene was larger than the control cells. In nude mice injected with MK-transfected or tMK-transfected cells, visible tumor was observed earlier and the tumor tissues were larger in size and weight than in control animals that were injected with cells without the transfection of either genes. CONCLUSION： Over-expressed MK or tMtC can promote human gastric cancer cell growth in vitro and in vivo, and bMK has greater effect than MK. tMK may be a more promising gene therapeutic target compared with MK for treatment of malignant tumors.

关键词： Midkine Truncated midkine Gastric cancer BGC823 Tumorigenisis

Techniques of copper recovery from Mexican copper oxide ore

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Mining Science and Technology 2009年第1期19卷 45-48页

作者： CAO Zhan-fang ZHONG Hong LIU Guang-yi zhao shu-juan Institute of Chemistry and Chemical Engineering Central South University Changsha Hunan 410083 China

Mexican copper ore is a mixed ore containing mainly copper oxide and some copper sulfide that responds well to flotation. The joint techniques of flotation and leaching were studied. The results indicate that an ore containing 19.01% copper could be obtained at a recovery ratio of 35.02% by using sodium sulfide and butyl xanthate flotation. Over 83.33% of the copper oxide can be recovered from the railings by leaching in suitable conditions, such as 1 h stirring at a temperature around 25 ℃with a mixing speed of S00 r/min, an H2SO4 concentration of 1.0 mol/L and a mass ratio of the ore-slurry-liquid to solid （mL/ms） of 3. The overall yield of refined ore after flotation and leaching is over 89.18% of the copper, which is much better than sole flotation or leaching. A copper product containing more than 99.9% copper was obtained by using the process： flotation-agitation leaching- solvent extraction-electro-winning.

关键词： copper oxide ore flotation stirring leaching extraction

Configuration Interaction of Electronic Structure and Spectroscopy of AlH and Its Cation

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Chinese Physics Letters 2021年第4期38卷 40-45页

作者： shu-Tao zhao Jun Li Rui Li shuang Yin Hui-Jie Guo School of Physics and Electronic Science Fuyang Normal UniversityFiiyang 236037China Institute of Atomic and Molecular Physics Jilin UniversityChangchun 130012China Department of Physics College of ScienceQiqihar UniversityQiqihar 161006China

We carry out a detailed study of the low-lying states of AlH and AlH^(+),using a multireference configuration interaction *** on the computed potential energy curves,the spectroscopic constants of bound A-S states are fitted;these agree with the results for the *** values of the permanent dipole moment of the A-S states are calculated,and the charge transfer mechanism is *** on the calculated transition dipole moments and vibrational levels,the radiative lifetimes of bound states are ***,tunneling lifetimes,and v’=0-2 vibrational levels of 4^(2)∑^(+) and 3^(2)Π states with a potential barrier are investigated.

关键词： method. vibrational dipole

Risk factors of recurrence in small-sized, node negative breast cancer in young women: a retrospective study in Chinese population

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Science China(Life Sciences) 2013年第4期56卷 335-340页

作者： ZHANG XiaoSan LI PengFei MA WenJie DI WenYu zhao shu GAO QingZu zhao YuYing YANG MaoPeng ZHANG QingYuan Department of Medical Oncology the Third Affiliated Hospital of Harbin Medical University Department of Radiology the Third Affiliated Hospital of Harbin Medical University Department of Pathology the First Affiliated Hospital of Xin Xiang Medical College Department of Medical Oncology the Fourth Affiliated Hospital of Harbin Medical University

We aimed to investigate risk factors of local and distant recurrence in small-sized, node negative breast cancer in women 〈35 years in a Chinese cohort. Between January 1994 and January 2007, 107 patients with pathologically confirmed small-sized （E1 cm）, node negative breast cancer who did not receive neoadjuvant or adjuvant chemotherapy were included. The 5-year recurrence-free survival （RFS） was estimated according to different prognostic variables. With a median time of 60 months （range, 8-60 months） follow-up, local and distant recurrence were observed in 25 cases （23.4%）. By univariate analysis, HER-2 positivity, triple negative （TN）, and high Ki-67 index （~14%） were risk factors of a lower RFS （hazard ratio （HR） 6.680, 95% confidence interval （CI） 2.350-18.985, P〈0.0001 for HER-2 positive; HR 4.769, 95%CI 1.559-14.591, P=0.006 for TN; HR 6.030, 95%CI 2.659-13.674, P〈0.0001 for high Ki-67 index）. Patients with grade 3 tumors had a lower RFS （HR 2.922, 95%CI 1.096-7.791, P=0.032） compared with those with grade 1 or grade 2 tumors. By multivariate analysis, HER-2 positivity （HR 10.204, 95%CI 3.391-30.704, P〈0.0001）, TN （HR 10.521, 95% CI 3.152-35.113, P〈0.0001） and high Ki-67 index （HR 10.820, 95%CI 4.338-27.002, P〈0.0001） remained risk factors of RFS. In this cohort, HER-2 positivity, triple neg- ative and high Ki-67 index were independent risk factors of RFS in young patients with Tla,bN0 breast cancer. Subsequent pregnancy did not affect RFS.

关键词： breast cancer recurrence-free survival risk factors young women

Weak absorption test and defect analysis of optical coatings

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光学精密工程 2005年第4期13卷 430-434页

作者： HE Hong-bo LI Xia FAN shu-hai zhao Yuan-an SHAO Jian-da FAN Zheng-xiu Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China

Surface thermal lensing technique was developed into a high-sensitive apparatus for weak absorption test and defect analysis of optical coatings. A continuous-wave 1 064 nm Nd:YAG laser and a He-Ne laser were employed as pump source and probe source, respectively. Low noise photoelectrical components and a lock-in amplifier were used for photo-thermal deformation signal detection. In order to improve sensitivity, the apparatus configuration was optimized by choosing appropriate parameters, including pump beam spot size, chopper frequency, detection distance, waist radius and position of probe beam. Coating samples were mounted on a x-y stage. Different procedures, such as single spot, linear scan and 2-dimension area scan, could be performed manually or automatically. Various optical coatings were prepared by both electron beam evaporation and ion beam sputtering deposition. High sensitivity was obtained and low to 1×10 -7 weak absorption was tested in low-loss coating samples. For the sensitivity extreme of the system, 1×10 -8 absorption was reason out to be measured by surface thermal lensing technique. Very small standard deviation was achieved for the reproducibility evaluation. Moreover, a spatial resolution of 25 micron was proved according to the area scan which traced out the profile of photo-thermal defects inside optical coatings. The system was employed in the analyses of optical absorption, absorption uniformity and defect characterization, and revealed the relationship between laser-induced damage and absorption of optical coatings.

关键词：光学涂覆技术吸收能力稳定性激光技术