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Chromatin-binding in vivo of the erythroid kruppel-like factor,EKLF,in the murine globin loci

Cell Research 2006年第4期16卷 347-355页

作者： Yu-Chiau shyu Shau-Ching Wen Tung-Liang Lee Xin Chen Chia-Tse Hsu Hsin Chen Ruei-Lin Chen Jau-Lang Hwang Che-Kun James Shen Institute of Molecular Biology Academia SinicaNankangTaipei 115 Institute of Life Sciences National Defense UniversityNei-huTaipei 114 Division of Biotechnology and Pharmaceutical Research National Health Research InstitutesZhunan TownMiaoli County 350 ~

EKLF is an erythroid-specific,zinc finger-containing transcription factor essential for the activation of the mamma-lian beta globin gene in erythroid cells of definitive *** have prepared a polyclonal anti-mouse EKLF antibodysuitable for Western blotting and immunoprecipitation(IP)qualities,and used it to define the expression patterns of theEKLF protein during mouse erythroid *** have also used this antibody for the chromatin-immunoprecipi-tation(ChIP)*** was found to bind in vivo at both the mouse beta-major-globin promoter and the HS2 site ofbeta-LCR in the mouse erythroleukemia cells(MEL)in a DMSO-inducible *** DMSO-induced bindings ofEKLF as well as three other proteins,namely,RNA polymerase Ⅱ,acetylated histone H3,and methylated histone H3,were not abolished but significantly lowered in CB3,a MEL-derived cell line with null-expression of p45/NF-E2,anerythroid-enriched factor needed for activation of the mammalian globin ***,binding of EKLF in vivowas also detected in the mouse alpha-like globin locus,at the adult alpha globin promoter and its far upstream regula-tory element alpha-MRE(HS26).This study provides direct evidence for EKLF-binding in vivo at the major regulatoryelements of the mouse beta-like globin gene clusters the data also have interesting implications with respect to the roleof EKLF-chromatin interaction in mammalian globin gene regulation.

关键词： EKLF mutine globin gene clusters chromatin-immunoprecipitation (CHIP) chromosome structure

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Anomaly detection of earthquake precursor data using long short-term memory networks

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Applied Geophysics 2019年第3期16卷 257-266,394页

作者： Cai Yin Mei-Ling shyu Tu Yue-Xuan Teng Yun-Tian Hu Xing-Xing Institute of Geophysics China Earthquake AdministrationBeijing 100081China Shandong Earthquake Agency Jinan 250014China Department of Electrical and Computer Engineering University of MiamiCoral GablesFL 33146USA.

Earthquake precursor data have been used as an important basis for earthquake *** this study,a recurrent neural network(RNN)architecture with long short-term memory(LSTM)units is utilized to develop a predictive model for normal ***,the prediction errors from the predictive models are used to indicate normal or abnormal *** additional advantage of using the LSTM networks is that the earthquake precursor data can be directly fed into the network without any elaborate preprocessing as required by other ***,no prior information on abnormal data is needed by these networks as they are trained only using normal *** using three groups of real data were conducted to compare the anomaly detection results of the proposed method with those of manual *** comparison results indicated that the proposed LSTM network achieves promising results and is viable for detecting anomalies in earthquake precursor data.

关键词： Earthquake precursor data deep learning LSTM-RNN prediction model anomaly detect io n

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The Effects of Nickel on Oxidative Stress,Apoptosis and Stress Proteins in Renal Cell Line

The Effects of Nickel on Oxidative Stress,Apoptosis and Stre...

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中国毒理学会第三届中青年学者科技论坛暨2011年全国前...

作者： Min-Jan Hsieh Huey-Wen shyu Department of Laboratory Sciences and Biotechnology

The toxicity of nickel has become of great interest because of the widespread environmental occurrence of nickel and the incidence of accidental poisoning in nickel worker. Exposure to nickel is linked with a risk of developing tumor of the *** purpose of this study was to investigate cytotoxicity,oxidative stress as an in vitro renal cell line model exposed to cytotoxic doses of *** order to elucidate the effects of nickel on oxidative impairments in renal cells,the level of intracellular oxidants,change of mitochondrial membrane potential,the level of intracellular calcium and apoptosis were measured in renal proximal tubule epithelial cell line(LLC-PK1)after nickel(Ⅱ)acetate *** nickel treatment,the decrease of viability showed a concentration-dependent *** and RNS levels were significantly increased in nickel-treated cells,Treatment of nickel resulted in decrease mitochondrial membrane potential which was accompanied by increasing of intracellular ***-induced LLC-PK1 death was significantly prevented by pretreatment with *** with cyclosporine A(a specific inhibitor of mitochondrial membrane potential)inhibited Ni-induced cell ***-induced cell death can also prevented by ruthenium red(a inhibitor of Cauptake by mitochondrial).In flow cytometric analysis,a rise in cell proportion in sub-G1 phase occurred in a concentration-dependent *** Ni treatment,there was reduced expression of Bcl-2 and Bcl-xL proteins,while induced cytochrome c,casp-9,-3 proteins *** to heavy metals is known to active the mechanism of defense which includes the production of stress proteins,*** shock proteins (HSPs),glucoseregulated proteins(GRPs)which can preserve cellular *** expression of stress protein on LLC-PK1 cells using western *** increase the expression of constitutive Hsp25 and inducible Hsp72,but it did not affect Grp78 compared to the control *** results su

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HOMELESSNESS AND URBAN SPRAWL OF LARGE CITIES OF THE U.S.

HOMELESSNESS AND URBAN SPRAWL OF LARGE CITIES OF THE U.S.

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作者： Amanda Kaserman shinming shyu Jiang Lu Eastern Michigan University

How to react to rapid expansion has become an essential issue for most large cities in not only the U.S.,but also the developing countries,such as China and *** authors consider how these Urban Sprawl affect,and are affected by,the rapidly population decline of the city center region and consequently followed by urban poverty;and attempt to

关键词： HOMELESSNESS AND URBAN SPRAWL OF LARGE CITIES OF THE U.S

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大鼠伤害性反应甩尾潜伏期(TFL)和嘶叫阈值(ST)的比较

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针刺研究 1985年第2期10卷 92-97页

作者：黄坤厚 shyu B.C. Andersson S.A. 中医研究院针灸研究所瑞典哥德堡大学生理研究所瑞典哥德堡大学生理研究所

甩尾和嘶叫反应的神经反射机制不同。用们对这二种伤害性反应的阈值变化作了比较。用正常Whistar Kyoto鼠(WKY)和原发性高血压鼠(SHR)分别于自由活动状态下和约束于管状鼠固定器中进行每20分钟测定,共一或二小时,观察ST和TFL的变化。并... 详细信息

甩尾和嘶叫反应的神经反射机制不同。用们对这二种伤害性反应的阈值变化作了比较。用正常Whistar Kyoto鼠(WKY)和原发性高血压鼠(SHR)分别于自由活动状态下和约束于管状鼠固定器中进行每20分钟测定,共一或二小时,观察ST和TFL的变化。并分别作静脉注入生理盐水、纳洛酮(1mg/kg)或吗啡(4mg/kg)的处理。结果WKY鼠(n=25)和SHR鼠(n=20)的TFL间无明显差异;在二小时测试期间TFL随时间延长无明显的变化,P>0.15。而ST均随时间的延长而明显增高。自由鼠(n=18)和约束鼠(n=24)ST都逐渐增高,40分钟时明显高于对照值,P0.4。纳洛酮使TFL明显缩短,P<0.005(n=13)。吗啡使ST及TFL均明显增加;P<0.01(n=9)。 ST随测试时间延长明显增高,而TFL无明显变化。表明二者神经机制不同。甩尾反应在动物脊髓化后仍可存在;而嘶叫反应需高级中枢参予与辨认过程有关。动物由自由转入约束状态后ST明显降低表明此时动物处于痛觉过敏状态。纳洛酮使二种处境的鼠ST和TFL均明显下降提示在二种状态下均存在一种鸦片镇痛系统的经常性兴奋。

关键词： TFL 纳洛酮 ST SHR 阈值门槛值

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Helicobacter pylori infection in relation to E-cadherin gene promoter polymorphism and hypermethylation in sporadic gastric carcinomas

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World Journal of Gastroenterology 2005年第33期11卷 5174-5179页

作者： Yao-Chi Liu Chen-Yang Shen Hurng-Sheng Wu De-Chuan Chan Cheng-Jueng Chen Jyh-Cherng Yu Cheng-Ping Yu Horng-Jyh Harn Rong-Yaun shyu Yu-Lueng Shih Chung-Bao Hsieh Huan-Mieng Hsu Division of General Surgery Department of Surgery Tri-Service General HospitalNational Defense Medical Center Taipei Taiwan China Division of Gastroenterology Department of Internal Medicine Tri-Service General HospitalNational Defense Medical Center Taipei Taiwan China Department of Pathology Tri-Service GeneralHospital National Defense Medical Center Taipei Taiwan China Department of Pathology Buddhist Tzu-Chi General Hospital Hualien Taiwan China Department of Surgery Show Chwan Memorial Hospital Changhua Taiwan China Institute of Biomedical Sciences Academia Sinica Taipei Taiwan China

AIM: To study Helicobacter pylori (H pylori) infection in relation to E-cadherin (E-cad) promoter polymorphism and hypermethylation in ***: Specimens were taken from representative cancerous lesions and adjacent non-cancerous epithelia of 67 resected GCs. Hpyloriwas detected by real-time PCR of the cagA gene from non-neoplastic epithelium.E-cad promoter polymorphism and hypermethylation were determined by restriction fragment length polymorphism analysis and methylation-specific PCR, respectively. Expression of E-cad protein was determined by ***: Hpyloriwas found in 57% of patients with GC.H pylori infection was more frequently found in tumors with the -160C/C genotype than those with the -160C/A and -160A/A genotypes (74% vs47%, P = 0.02). Hpylori infection was associated with E-cad methylation in nonneoplastic epithelium; however, no significant difference in H pylori was observed between methylated and unmethylated cancerous ***: Patients with the -160C/C genotype might require Hpyloriinfection to promote the inactivation of CDH1, suggesting that Hpylori infection might affect GC in an initial stage because polymorphism is germ *** of hypermethylation of CDH1 promoter in GC is complex, and Hpyloriinfection might affect it in an initial stage.

关键词： H pylort E-cadherirr -160 C→A polymorphism Hypermethylation Gastric carcinoma

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Ceramic Films via Organometallic Complex as Single Source Precursor

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合成化学 2004年第Z1期12卷 122-122页

作者： shyu shin-Guang Wu Chi-Chin Institute of Chemistry Academia Sinica Taipei Taiwan Department of Chemistry National Chung Cheng University Chia-Yi TaiwanInstitute of Chemistry Academia Sinica Taipei Taiwan

Fe2(CO)6(μ-S2) was used as a single source precursor in attempt to produce FeS film via MOCVD. Pyrolysis of Fe2(CO)6(μ-S2) at temperature below 500℃ produced Fe1-xS or Fe7S8 powder as indicated by its powder X-ray spectra. At 750 ℃, polycrystalline FeS powder was obtained. In film deposition, polycrystalline Fe1-xS or Fe7Ss films were obtained on Si(100) and Ag/Si(100) substrates below 500 ℃. SEM micrographs showed the film on Si(100) substrate containing whisker like grains. However, pillar like grains were obtained on Ag/Si(100) *** rates are also different for different substrates as evaluated by the thickness of the films, which were obtained by SEM micrographs of the cross section of the films. At 750℃, similar polycrystalline Fe1-xS or Fe7S8 film was obtained.

关键词： MOCVD, FeS film, Single Source Precursor.

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Manufacturing of micro tungsten carbide electrode using supersonic aided electrolysis process

Manufacturing of micro tungsten carbide electrode using supe...

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2007年上海市电子电镀学术年会

作者： Feng-Tsai Weng Ray-fang shyu Chi-Ting Ho Institute of Mechanical and Electrical-mechanical Engineering National Formosa University 64 Wen-Hua Road Huwei YunlinTaiwan Institute of Mechanical and Electrical-mechanical Engineering National Formosa University 64 Wen-Hua Road Huwei YunlinTaiwan Institute of Mechanical and Electrical-mechanical Engineering National Formosa University 64 Wen-Hua Road Huwei YunlinTaiwan

＜正＞A noble micromachining technology was developed for the fabrication of micro parts using a nontraditional machining process in this *** carbide rods were selected as *** original diameter of tungsten carbide rod was 3mm,it was processed to 50μm using precision grinding process in first step,and then the rod could be machined to the final desired diameter on the anode in supersonic aided ***-aspect-ratio of micro tungsten carbide rod was easily obtained by this *** roughness of the sample with supersonic aided agitation was compared with that with no agitation in *** machined surface of the sample is very smooth due to ionized particles of anode could be removed by supersonic aided agitation during *** with a tip of about 1/am could be obtained by this process.

关键词： Micromachining Tungsten carbide Electrode Electrolysis Supersonic

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剥蚀对活动逆冲断层地震活动性的影响

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地壳构造与地壳应力 2018年第1期5卷 23-32页

作者： Philippe Steer Martine Simoes Rodolphe Cattin J.Bruce H.shyu 王璞(翻译) 黄禄渊(校对) Geosciences Rennes Universite Rennes 1 and CNRS UMR 6118RennesFrance Institut de Physique du Globe de Paris Sorbonne Paris CiteUniversity Paris DiderotUMR 7154 CNRSF-75005 ParisFrance Geosciences Montpellier Universite Montpellier Ⅱ and CNRS UMR 5243France Department of Geosciences National Taiwan UniversityTaipei 10617Taiwan 不详

评估地震危害仍然是地球科学中最具挑战性的科学问题之一。经典理论认为深部构造运动是驱动地震活动断层上的应力持续加载的唯一机制。这里我们通过一个力学模型显示,在一个地震周期的时间范围内,剥蚀也会显著影响逆冲断层的应力加载。... 详细信息

评估地震危害仍然是地球科学中最具挑战性的科学问题之一。经典理论认为深部构造运动是驱动地震活动断层上的应力持续加载的唯一机制。这里我们通过一个力学模型显示,在一个地震周期的时间范围内,剥蚀也会显著影响逆冲断层的应力加载。事实上,例如台湾和其他活动逆冲造山带约0.1~20mm/a的剥蚀率就可以将震中附近的逆冲断层的库仑应力提高约0.1~10bar。在连续的或短暂和强烈的地震过程中,地表所发生的物质转移是地表附近断层的震间应力加载的主要机制。这种应力可能足以引发浅层地震活动或促进大陆深部地震的破裂一直延续到地表。

关键词：地震活动性逆冲断层剥蚀率库仑应力深部地震科学问题地球科学地震危害

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2010年台湾心脏病学会高血压管理指南

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中华高血压杂志 2011年第1期19卷 9-23页

作者： Chern-En Chiang Tzung-Dau Wang Yi-Heng Li Tsung-Hsien Lin kuo-Liong Chien Hung-I Yeh Kou-Gi shyu Wei-Chuen Tsai Ting-Hsing Chao Juey-Jen Hwang Fu-Tien Chiang Jyh-Hong Chen Unknown unit

高血压是心血管疾病和心血管死亡的重要危险因素。从2003年美国高血压预防、检测、评价和治疗全国联合委员会第7次报告（the seventh report of the joint national committee on prevention,detection,evaluation,and treatment of hig... 详细信息

关键词：欧洲心脏病学会高血压协会管理指南 prevention 心血管疾病台湾地区联合委员会危险因素

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