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High-throughput analysis of anthocyanins in horticultural crops using probe electrospray ionization tandem mass spectrometry (PESI/MS/MS)

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Horticulture Research 2023年第4期10卷 249-258页

作者： Misaki Ishibashi Kei Zaitsu Ikue Yoshikawa shungo otagaki Shogo Matsumoto Akira Oikawa Katsuhiro Shiratake Graduate School of Bioagricultural Sciences Nagoya UniversityChikusaNagoyaAichi 464-8601Japan Graduate School of Agriculture Kyoto UniversitySakyoKyoto 606-8502Japan Faculty of Biology-Oriented Science and Technology Kindai UniversityNishimitaniKinokawaWakayama 649-6493Japan Faculty of Agriculture Meijo UniversityTenpakuNagoyaAichi 468-8502Japan

Plant secondary metabolites exhibit various horticultural *** and rapid analysis methods for evaluating these metabolites are in demand in breeding and consumer markets dealing with horticultural *** applied probe electrospray ionization(PESI)to evaluate secondary metabolite levels in horticultural *** does not require pre-treatment and separation of samples,which makes it suitable for high-throughput *** this study,we targeted anthocyanins,one of the primary pigments in horticultural ***-one anthocyanins were detected in approximately 3 minutes in the selected reaction-monitoring *** mass spectrometry(MS/MS)could adequately distinguish between the fragments of anthocyanins and *** sampling,an intuitive method of sticking a probe directly to the sample,could detect anthocyanins qualitatively on a micro-area scale,such as achenes and receptacles in strawberry *** results suggest that PESI/MS/MS can be a powerful tool to characterize the profile of anthocyanins and compare their content among cultivars.

关键词： MS/MS ionization dealing

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Efficient Breeding and Cultivation of Type 2 Red-fleshed Apple Cultivars Using a Search System for Suitable Apple Cultivar Combination

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Horticultural Plant Journal 2018年第6期4卷 219-225页

作者： SHOGO Matsumoto LI Tianzhong shungo otagaki LI Yang BAI Songling Graduate School of Bioagricultural Sciences Nagoya University Laboratory of Fruit Cell and Molecular Breeding China Agricultural University Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang University

As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages. All of the investigated type 2 red-fleshed apples contained the S3-RNase allele closely linked to the red-fleshed trait. We made a search system for ovule parents to efficiently produce new type 2 red-fleshed cultivars based on the S-RNase allele information(http://***/～hort/apple/red/). More than 90% of progenies from crossing a selected white-fleshed ovule parent with a type 2 red-fleshed pollen parent are expected to show the red-fleshed phenotype. We also compiled a database of the S-RNase genotypes of more than 1 000 apple cultivars, including a survey system of cultivar combinations showing those that were fully incompatible, semicompatible and fully compatible, written in Chinese(http://***/～hort/apple/ch/) and Japanese(http://***/～hort/apple/ja/).

关键词： Malus×domestica type 2 red-fleshed S-RNase genotype cultivar combination self-incompatibility

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