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Prokaryotic Expression of Gly-Gln Dipeptide and Its Bioactive Analysis: A Novel Method for Short Peptide Production

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Agricultural Sciences in China 2010年第5期9卷 736-744页

作者： XU Ping-wen FANG Xin-ling shu gang ZHU Xiao-tong LUO Zeng-fu JIANG Qing-yan GAO Ping ZHANG Yong-liang College of Animal Science South China Agricultural University Guangzhou 510642 P.R.China

Small bioactive peptides, with diverse biological functions, have received increasing attention as physiologically beneficial substances in animal production. The main obstacle to wide application of small bioactive peptides is a lack of costeffective methods for mass production. In this study, we mass-production method for small bioactive peptides. used glycyl-glutamine （Gly-Gln） as a test case to develop a novel The oligonucleotide encoding Gly-Gln pro-peptide （Gpp） was designed and synthesized. Gpp includes 3 Gly-Gln dipeptides and 2 enzymatic sites for pepsin and trypase, allowing direct digestion and absorption of Gly-Gln in the gastrointestinal tract. The Gpp oligonucleotides were linked to generate an oligomeric oligonucleotide segment containing 12 tandem copies of Gpp. This 12Gpp segment was cloned and expressed in Escherichia coli vector pET32a. By optimizing culture conditions [0.1 mmol L^-1 isopropyl-β-D- thiogalactopyranoside （IPTG）, 50 μg mL^-1 ampicillin （Amp）, 30℃ for 12 h], the thioredoxin fusion peptides reached 40% of total bacterial protein. After purification, the fusion protein was fed to Kunming mice to determine its effect on mouse immune function. The results showed that similar to Gly-Gln dipeptide, Gpp polymer protein could significantly suppress the proliferation of T and B lymphocytes in blood and spleen, and additionally could significantly improve interleukin-2 （IL-2） and interleukin-6 （IL-6） secretion of blood and spleen lymphocytes. These effects were not observed in mice fed a 2 amino acids mix （glycine and glutamine）. These evidences indicated that an efficient digestion of Gpp polymer protein could be achieved when ingested into the animal gut. The expression system in this study provides a potential production method for not only Gly-Gln dipeptide but also other short bioactive peptides.

关键词： glycyl-glutamine tandem expression gastrointestinal tract

Effectiveness analysis of methane-drainage by deep-hole controlled pre-splitting blasting for preventing coal and gas outburst

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Journal of Coal Science & Engineering(China) 2009年第2期15卷 166-170页

作者： CAO shu-gang LI Yong LIU Yan-bao ZHANG Li-qiang XU A-meng Key Laboratory for Exploitation of Southwest Resources and Environmental Disaster Control Engineering Ministry of Education Chongqing University Chongqing 400044 China Safety Engineering College North China Institute of Science and Technology Beijing 101601 China

In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal masses produce many irreversible ***,the nearer the distance from blasting hole,the larger the BET surface areaand volume ratio of the infiltration pore are;they increased by 11.47%and 5.73%,*** coefficient of air permeability is increased 4 *** 3 months,the gasdrainage rate was increased by 66%.In the first 15 days,the cumulative pumped gas was1.93 times of blasting *** average absolute gas emission decreased by 63.46%.Experimental results show that deep-hole controlled pre-splitting blasting not only preventscoal and gas outburst,but also gives good economic results.

关键词： coal and gas outburst methane-drainage pore structure deep-hole controlled pre-splitting blasting

Shenxian:Living by the Fruits of Their Labors

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China Today 1994年第4期43卷 22-25页

作者： $$$$YONG gang and shu BIAO

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Ｓｈｅｎｘｉａｎ：ＬｉｖｉｎｇｂｙｔｈｅＦｒｕｉｔｓｏｆＴｈｅｉｒＬａｂｏｒｓＩｎｔｒｏｄｕｃｉｎｇａｃｏｍｍｕｎｉｔｙｔｈａｔｒｕｎｓｏｎ＂ｐｅａｃｈｐｏｗｅｒ．＂ＳＨＥＮＸＩＡＮＣＯＵＮＴＹ－ｃａｌｌｅｄＳｈｅｎｚｈｏｕｉｎａｎｃｉｅｎｔｔｉｍｅ...

关键词： Shenxian:Living by the Fruits of Their Labors

Social Origins and Political Participation of Private Business Owners in Beijing

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Fudan Journal of the Humanities and Social Sciences 2010年第4期3卷 22-41页

作者： Bjom Alpermann gang shu-ge

Over the past 30 years, Chinese society has undergone an enormous social transition. Along with the rise of a private economy, private business owners have emerged as a new social stratum, which attracts academic attention domestically and internationally. As for the social origins of the private business owners, some of the private business owners are former cadres and current members of the Chinese Communist Party（CCP）. A question rarely pursued is what effects these ties have on political participation of＂ private business owners. The paper explores this question on the basis of data gathered in a representative survey of private enterprises in 2006. The study shows that the Chinese private business owners have diverse social origins, and their behavior in terms of political participation is also distinct in some ways. When it comes to the patterns of political participation of the private business owners, CCP members and former officials among private business owners indeed have unique characteristics in their access to political resources, but the patterns of political participation vary between these two groups. Furthermore, there are also considerable differences between members of the CCP and democratic parties in China in these respects. These findings cast doubt on the common use of CCP membership and occupational experience as cadre as interchangeable indicators for the ＂political capital＂ of business owners. A more differentiated understanding of the nature of political capital and mechanisms by which it is put to use by private entrepreneurs in China is called for.

关键词： private business owners social origins political participation social transition

A Study on Agricultural Land Consolidation Potential in Changfeng County

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Asian Agricultural Research 2017年第4期9卷 66-68,71页

作者： gang shu Zhongxiang YU College of Economics and Management Anhui Agricultural University Institute of Land and Resources Anhui Agricultural University

In this paper,we study the agricultural land consolidation quantity potential( including theoretical quantity potential and actual quantity potential) and quality potential in Changfeng County,use the new arable land area to denote the quantity potential,and use the food production improved after agricultural land consolidation to represent quality potential. On this basis,we measure and evaluate the agricultural land consolidation potential in Changfeng County.

关键词： Agricultural land Consolidation potential Changfeng County

Long-term ocean temperature trend and marine heatwaves

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Journal of Oceanology and Limnology 2024年第4期42卷 1037-1047页

作者： Min ZHANG Yangyan CHENG gang WANG Qi shu Chang ZHAO Yuanling ZHANG Fangli QIAO First Institute of Oceanography Ministry of Natural ResourcesQingdao 266061China Key Laboratory of Marine Sciences and Numerical Modelling Ministry of Natural ResourcesQingdao 266061China Shandong Key Laboratory of Marine Science and Numerical Modeling Qingdao 266061China

Marine heatwaves(MHWs)can cause irreversible damage to marine ecosystems and *** MHW characterization remains difficult,because the choice of a sea surface temperature(SST)temporal baseline strongly influences MHW *** a recent work suggesting that there should be a communicating baseline for long-term ocean temperature trends(LTT)and MHWs,we provided an effective and quantitative solution to calculate LTT and MHWs simultaneously by using the ensemble empirical mode decomposition(EEMD)*** long-term nonlinear trend of SST obtained by EEMD shows superiority over the traditional linear trend in that the data extension does not alter prior *** MHWs identified from the detrended SST data exhibited low sensitivity to the baseline choice,demonstrating the robustness of our *** also derived the total heat exposure(THE)by combining LTT and *** THE was sensitive to the fixed-period baseline choice,with a response to increasing SST that depended on the onset time of a perpetual MHW state(identified MHW days equal to the year length).Subtropical areas,the Indian Ocean,and part of the Southern Ocean were most sensitive to the long-term global warming trend.

关键词： marine heatwaves(MHWs) ensemble empirical mode decomposition(EEMD) long-term temperature(LTT)trend total heat exposure(THE)

Synthesis of Enantiom eric Planar Square Ni^(2+) -and Cu^(2+) -Schiff Base Com plexes

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Chemical Research in Chinese Universities 1999年第3期15卷 248-252页

作者： ZHAO Jian-zhang WANG shu-gang WANG Xu LIU Ju-zheng QIAO Xiao-guang LI Yao-xian WANG Hua Department of Chemistry Jilin UniversityChangchun 130023P.R.China Third Clinical College&China Japan Union Hospital of Norman Bethune Unviersity of Me dical Sciences Changchun 130023P.R.China

Enantiomeric four chelated planar square schiff base Cu 2+ and Ni 2+ complexes were synthesized using ( 1R,trans ) and ( 1S,trans) N,N  bis(2 hydroxyphenyl methylene) 1,2 diaminocyclohexane. The chirality could not be shown by usual UV Vis spectra. The intrinsic chiral difference of the enantiomeric complexes can be displayed by circular dichroism(CD) spectra. The Cotton effect of every polarlight sensitive absorption of one enantiomer will be the opposite of that of the other enantiomer. It was found that no tetrahedron form exists for Ni 2+ complexes, which was determined with UV Vis spectra and Electron Paramagnetic Resonance(EPR) measures.

关键词： Chiral transition metal schiff base complex Circular dichroism(CD) Enantiomeric

The Up-conversion Laser Properties of a Non-polar Organic Dye and the Influence of Solvents and Pumping Wavelengths

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Chinese Chemical Letters 2006年第1期17卷 81-84页

作者： Xiao Qiang YU Xian ZHANG Gui Bao XU Hua Ping ZHAO Wei HE Zong shu SHAO Xian gang XU Min Hua JIANG The National Key Lab of Crystal Materials Shandong University Jinan 250100

A non-polar organic dye, E, E-1, 4-bis[4＇-（N,N-dibutylamino）styryl]-2,5-dimethoxybenzene （DBASDMB）, has been synthesized and characterized, and its structure has been determined. Pumped with a 200fs pulse this dye showed the up-conversion laser properties. The influences of various organic solvents and different pumping wavelength on the laser properties have been demonstrated.

关键词： Up-conversion laser two-photon absorption non-polar organic dye.

Single-stage Posterior Spondylectomy, Circumferential Decompression and Reconstruction Using Mesh Cage for Spinal Tumors

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Chinese Medical Sciences Journal 2009年第3期24卷 172-177页

作者： Yong Zhu Hong Zhao Giu-xing Qiu Jian-guo Zhang Ye Tian shu-gang Li Suo-mao Yuan Department of Orthopedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To explore the feasibility and clinical results of circumferential decompression and three column reconstruction through single-stage posterior transpedicular approach for spinal tumor treatment. Methods Totally, 24 patients with spinal tumor underwent tumor resection and spinal reconstruc tion through single-stage posterior transpedicular approach. Preoperatively, according to the Frankel classification, 12 patients were grade E, 9 grade D, and 3 grade C. Anterior column was reconstructed with non-expandable titanium cages. Posterior segmental instrumentation was used to maintain the stability of spine in all cases. Anterior and posterolateral fusion was performed with autograft and allogenic bone. The following data were followed up in these patients: deformity angle, local recurrence, neurological function, and spinal bony fusion. Results The average operating time and blood loss was 5.6 hours and 3 400 ml respectively. No intraoperative and postoperative complications were observed in this group. Postoperatively, 21 patients were Frankel grade E, 2 grade D, and 1 grade C. Four patients reported significant functional restoration and twenty patients reported complete resolution of pain. At follow-up (range, 6-42 months), implant failure or recurrent neurological symptoms was not found. Conclusions The tumor resection and spinal reconstruction through single-stage posterior transpedicular approach is a safe and effective technique for the treatment of spinal tumor. It can fully decompress the neurological structures, correct the kyphosis, and achieve early weight-bearing. This technique can improve life quality for the patients with spinal tumor.

关键词： spinal tumor vertebrectomy spinal reconstruction