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中国兽药杂志 1986年第3期 35-38页

作者： YUTAKA TAMURA HIROTAKA MAKIE shozo tanaka 蒋玉文日本国家兽医监测实验室第一分部

提出了一种以超声波处理的浸出液为抗原的间接血凝试验(IHA),来测定气肿疽梭菌的抗体。这种抗原能够吸附到经鞣酸处理过的,戍二醛固定的绵羊红细胞上,并能被胰酶和热处理破坏。用 SDS—聚丙烯酰胺凝胶电泳和凝胶扩散试验对抗原进行分析... 详细信息

提出了一种以超声波处理的浸出液为抗原的间接血凝试验(IHA),来测定气肿疽梭菌的抗体。这种抗原能够吸附到经鞣酸处理过的,戍二醛固定的绵羊红细胞上,并能被胰酶和热处理破坏。用 SDS—聚丙烯酰胺凝胶电泳和凝胶扩散试验对抗原进行分析时,结果与细菌鞭毛抗原完全一致。当这种抗原与4种梭菌抗体作 IHA 试验时,未发现有血清学交叉反应。我们的结果表明,间接血凝试验主要检测气肿疽梭菌鞭毛的抗体。

关键词：间接血凝试验被动血凝反应气肿疽梭菌气肿疽梭状芽孢杆菌 IHA 抗原抗体

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Efficacy and safety of dual therapy with daclatasvir and asunaprevir in elderly patients

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World Journal of Hepatology 2017年第11期9卷 544-550页

作者： Kazuo Tarao Katsuaki tanaka Akito Nozaki Akira Sato Toshiya Ishii Hirokazu Komatsu Takaaki Ikeda Tatsuji Komatsu shozo Matsushima Kenji Oshige Tarao’s Gastroenterological Clinic Gastroenterological Center Medical Center Yokohama City University Division of Gastroenterology Department of Internal Medicine St. Marianna University Yokohama City Seibu Hospital Department of Gastroenterology Yokohama Municipal Citizen’s Hospital Department of Gastroenterology Yokosuka General Hospital Uwamachi Department of Clinical Research National Hospital Organization Yokohama Medical Center Yokohama National University Center for Health Service Sciences

To survey the efficacy and safety of dual therapy with daclatasvir and asunaprevir in the elderly hepatitis C virus (HCV) patients multicentricity. METHODSInterferon-ineligible/intolerant patients and non-responders to previous pegylated-interferon/ribavirin therapy with chronic HCV genotype 1b infection were enrolled. Child B, C cirrhotic patients were excluded. Patients received oral direct acting antiviral treatment consisting of 60 mg daclatasvir once daily plus 200 mg asunaprevir twice daily for 24 wk. We divided the patients into two groups of 56 elderly patients (≥ 75 years-old) and 141 non-elderly patients (24). In the former, 1.8% experienced viral breakthrough, as compared with 3.5% in the latter (not significant). Adverse events occurred in 55.4% of the former and 56.0% of the latter. In the former, 7 cases (12.5%) were discontinued due to adverse events, and in the latter 9 cases were discontinued (6.4%, not significant). CONCLUSIONDual therapy with daclatasvir and asunaprevir achieved the same high rates of SVR24 in HCV elderly patients without more adverse events than in the non-elderly patients.

关键词： Asunaprevir Chronic hepatitis Daclatasvir Dual oral therapy Elderly patients Hepatitis C virus infection Hepatitis C virus Liver cirrhosis

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Use of low-phosphorus feed for Biwa salmon aquaculture

Use of low-phosphorus feed for Biwa salmon aquaculture

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The 14th International Symposium on Fish Nutrition & Feeding

作者： shozo Sugiura Takashi Taguchi Akira Yamaoka Hidetomo tanaka Kunihiko Kuwamura Department of Bioresources Management University of Shiga Prefecture

Biwa salmon,Oncorhynchus masou rhodurus,is endemic to Lake Biwa,and is considered one of the tastiest fishes among ***,aquaculture of this species is still in its ***,we conducted the present study to develop eco-friendly feed for Biwa salmon *** salmon(1.5 year-old,Samegai strain,initial mean body wt.277g) were reared in FRP tanks(1mt ton) and also in concrete ponds for 3 *** were fed either LP feed(low-phosphorus (P),fish meal-free diet;total P 0.73%,CP 39%,fat 16%) or control feed(commercial feed for Masu salmon;total P 1.61%,CP 45%,fat 6%) once daily to apparent satiation. Availability of dietary P was determined in vivo in separate *** groups of fish showed comparable feed consumption on an energy basis;however,toward the end of feeding duration feed intake decreased due to sexual *** mean body wt was 370g and 392g for LP and control groups,respectively(p>0.05).Both groups of fish showed similar feed efficiency (48-61%).Carcass P content was similar for both groups of fish,and was within a normal range. Fat content of fish fillets was 8.9%(LP) and 7.6%(control)(p0.05).The mRNA expression of phosphate-transporter(NaPi2b) in the colon was higher in control than LP fish (p0.05).Effluent P concentration was 10.7-18.2ppb in LP tanks,and 23.8-35.9ppb in control tanks(p<0.05).However,using a stagnant water system,net P excretion over 6 hours became Oppb in LP and 118.3ppb in control tanks (p<0.01).Apparent availability of dietary P was 50.8%(LP) and 28.8%(control).Estimated P

关键词： Phosphorus Low-pollution Biwa salmon Sustainable feeds

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