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Upconversion Nanoparticles-Encoded Hydrogel Microbeads-Based Multiplexed Protein Detection

Nano-Micro Letters 2018年第2期10卷 135-150页

作者： Swati shikha Xiang Zheng Yong Zhang Department of Biomedical Engineering Faculty of Engineering National University of Singapore (NUS) NUS Graduate School for Integrative Sciences and Engineering Centre for Life Sciences (CeLS)

Fluorescently encoded microbeads are in demand for multiplexed applications in different *** to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) are better alternatives due to their large antiStokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate(PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swellingbased encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence,we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their *** to functionalize the surface of PEGDA microbeads(acrylic acid incorporation, polydopamine coating)reported thus far quench the fluorescence of UCNPs. Here,PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were *** color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin(HSA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein(hCRP) and HSA protein by immobilizing anti-h CRP antibodies on green UCNPs.

关键词： Upconversion nanoparticles PEGDA microbeads Encoding Multiplexed bio-detection Single wavelength excitation

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Fate and toxicity of nanoparticles in aquatic systems

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Acta Geochimica 2023年第1期42卷 63-76页

作者： shikha Singh Sheo Mohan Prasad Gausiya Bashri Ranjan Plant Physiology and Biochemistry Laboratory Department of BotanyUniversity of Allahabad211002 AllahabadIndia Department of Botany Aligarh Muslim University202001 AligarhIndia

Nanotechnology is a ground-breaking multidisciplinary fi eld across a broad spectrum of basic and applied sciences for producing and applying nano-sized materials for innovative *** use of nanomaterials in industrial applications,medical products,and consumers has increased repeatedly over the last few years,and these applications will likely continue to *** an aquatic ecosystem,nanoparticles take entry through a direct route that includes industrial discharge,disposal of wastewater treatment e ffl uents,and indirect runof f from the *** reaching the aquatic environment,the nanomaterials are highly af fected by their backdrops and subsequently go through various conversions like agglomeration,aggregation,dissolution,sulfi dation,*** fate and the behavior of nanomaterials in the aquatic system not only depend on their physical-chemical properties but also on the pH,temperature,salinity,water hardness,and concentration of natural organic matter present in receiving *** this review,emphasis has been given to the toxicological properties and potential risks of nanomaterials in terms of factors contributing to their toxicology,bioavailability,and accumulation in aquatic organisms as well as the ***,we summarize the published data on engineered nanoparticles’ef fect on aquatic *** issues related to the accumulation and penetration of nanoparticles in the aquatic organism,their toxic ef fect,and biotransformation along with the food web are also *** nanomaterials are being increasingly released into aquatic bodies,it is important to pay greater attention to their toxicity and how it af fects the aquatic *** nanomaterials are bioavailable to plants,resulting in trophic transfer,and they impact other organisms through biomagnifi cation,as discussed in this *** close the enormous information gap,extensive research on the interactions and impacts of NPs on dif ferent species belonging to di

关键词： wastewater sized likely

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Electronic structure factors and the importance of adsorbate effects in chemisorption on surface alloys

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npj Computational Materials 2022年第1期8卷 1560-1571页

作者： shikha Saini Joakim Halldin Stenlid Frank Abild-Pedersen SUNCAT Center for Interface Science and Catalysis Department of Chemical EngineeringStanford University443 Via OrtegaStanfordCA94305USA SUNCAT Center for Interface Science and Catalysis SLAC National Accelerator Laboratory2575 Sand Hill RoadMenlo ParkCA94025USA

The chemisorption energy is an integral aspect of surface chemistry,central to numerous fields such as catalysis,corrosion,and ***-structure-based methods such as the Newns-Anderson model are therefore of great importance in guiding the engineering of material surfaces with optimal ***,existing methods are inadequate for interpreting complex,multi-metallic ***,we introduce a physics-based chemisorption model for alloyed transition metal surfaces employing primarily metal d-band properties that accounts for perturbations in both the substrate and adsorbate electronic states upon ***,we show that adsorbate-induced changes in the adsorption site interact with its chemical environment leading to a second-order response in chemisorption energy with the d-filling of the neighboring *** demonstrate the robustness of the model on a wide range of transition metal alloys with O,N,CH,and Li adsorbates yielding a mean absolute error of 0.13 eV versus density functional theory reference chemisorption energies.

关键词： alloys corrosion chemisorption

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A review of imaging techniques for plant disease detection

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Artificial Intelligence in Agriculture 2020年第1期4卷 229-242页

作者： Vijai Singh Namita Sharma shikha Singh GL Bajaj Institute of Technology and Management Gr.NoidaUttar PradeshIndia

Agriculture is the basis of every economy *** production is one of the major factors affecting domestic market condition in any *** production is also a major prerequisite of economic development,be it any part of any *** plays a crucial role as it even provides raw material,employment and food to different citizens.A lot of issues are responsible for estimated crop production varying in different parts of the *** of these include overutilization of chemical fertilizers,presence of chemicals in water supply,uneven distribution of rainfall,different soil fertility and *** than these issues one of the commonly faced challenges across the globe equally includes destruction of themajor part of production due to *** providing effective resources to the fields,major section of the production is diminished by the presence of diseases in the plants *** leads to focus on effective ways of detection of disease in *** of various diseases in plant is a major concern among *** diseases acts as a major threat to small scale farmers as they lead tomajor destruction in overall food *** provide effectivemeasures for detection and avoidance of the destruction requires an early identification of type of plant disease *** recent timemajorwork is being done for the identification of plant disease presents in varied parts of theworld affection varied *** is being done in the domain of identification of causing factors of these *** the diseases are marked by the presence of viruses while some are resultant of fungal *** becomes a major issuewhen the causing factor is not traceable before it has already spread to major production *** paper brings a review on effective use of different imaging techniques and computer vision approaches for the identification and classification of plant *** of Plant disease is initiated with image a

关键词： Plant disease detection Machine learning Imaging sensors and systems Plant disease classification Image processing

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Targeting cancer testis antigens for biomarkers and immunotherapy in colorectal cancer: Current status and challenges

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World Journal of Gastrointestinal Oncology 2015年第12期7卷 492-502页

作者： Anil Suri Nirmala Jagadish shikha Saini Namita Gupta Cancer Microarray Genes and Proteins Laboratory National Institute of Immunology

Colorectal cancer ranks third among the estimatedcancer cases and cancer related mortalities in United States in 2014. Early detection and efficient therapy remains a significant clinical challenge for this disease. Therefore, there is a need to identify novel tumor asso-ciated molecules to target for biomarker development and immunotherapy. In this regard, cancer testis antigens have emerged as a potential targets for developing novel clinical biomarkers and immunotherapy for various malignancies. These germ cell specific proteins exhibit aberrant expression in cancer cells and contribute in tumorigenesis. Owing to their unique expression profile and immunogenicity in cancer patients, cancer testis antigens are clinically referred as the most promising tumor associated antigens. Several cancer testis antigens have been studied in colorectal cancer but none of them could be used in clinical practice. This review is an attempt to address the promising cancer testis antigens in colorectal cancer and their possible clinical implications as biomarkers and immunotherapeutic targets with particular focus on challenges and future interventions.

关键词： Cancer testis antigens Colorectal cancer Testis sp

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Pasteurella multocida bacteremia in a patient with septic arthritis

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Infectious Medicine 2022年第3期1卷 221-223页

作者： shikha Malhotra Tung Phan Department of Pathology University of PittsburghPittsburghPennsylvaniaUSA

Pasteurella multocida is a common commensal microorganism found in the nasopharynx of domestic and wild *** acquire P multocida infection primarily through contact with animals or their mucous secretions.P multocida is infrequently encountered in clinical settings,and it is considered as a zoonotic *** this study,we present an interesting case of septic arthritis and bacteremia caused by P multocida in a 62-year-old *** patient was treated with surgical procedures and antibiotic therapy,which made significant *** case study highlights the importance of P multocida in causing zoonotic infection in humans.

关键词： Bacteremia Arthritis Pasteurella mulocida Total knee replacement

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Modelling and Analysis of Bacteria Dependent Infectious Diseases with Variable Contact Rates

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Computers, Materials & Continua 2021年第8期68卷 1859-1875页

作者： J.B.Shukla shikha Singh Jitendra Singh Sunil Kumar Sharma Innovative Internet University for Research Kanpur208017India P.P.N.(P.G.)College CSJM UniversityKanpur208001India College of Computer and Information Sciences Majmaah UniversityMajmaah11952Saudi Arabia

In this research,we proposed a non-linear SIS model to study the effect of variable interaction rates and non-emigrating population of the human habitat on the spread of bacteria-infected *** assumed that the growth of bacteria is logistic with an intrinsic growth rate is a linear function of *** this model,we assume that contact rates between susceptibles and infectives as well as between susceptibles and bacteria depend on the density of the non-emigrating population and the total population of the *** stability theory has been analyzed to analyzed to study the crucial role played by bacteria in the increased spread of an infectious *** is shown that as the density of non-emigrating population increases,the spread of an infectious disease *** is shown further that as the emigration increases,the spread of the disease decreases in both the cases of contact mentioned above rates,but this spread increases as these contact rates *** suggested that the control of bacteria in the human habitat is very useful to decrease the spread of an infectious *** results are confirmed by numerical simulation.

关键词： Mathematical modelling density dependent contact rates stability analysis

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Insect feeding deterrent and growth inhibitory activities of scopoletin isolated from Artemisia annua against Spilarctia obliqua （Lepidoptera： Noctuidae）

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Insect Science 2011年第2期18卷 189-194页

作者： Arun Kumar Tripathi Rajendra Singh Bhakuni shikha Upadhyay Rashmi Gaur Central Institute of Medicinal and Aromatic Plants Lucknow India

Artemisia annua （Asteraceae） is well known for its antimalarial activities due to presence of the compound artemisinin. We isolated a methoxy coumarin from the stem part of A. annua and confirmed its identity as scopoletin through mass spectral data. The structure was established from 1H-nuclear magnetic resonance （NMR）, 13C-NMR. The compound scopoletin was evaluated for its feeding deterrence and growth inhibitory potential against a noxious lepidopteran insect, Spilartctia obliqua Walker. Scopoletin gave FD50（feeding deterrence of 50%） value of 96.7 μg/g diet when mixed into artificial diet. S. obliqua larvae （12-day-old） exposed to the highest concentration （250μg/g diet） of scopoletin showed 77.1% feeding-deterrence. In a growth inhibitory assay, scopoletin provided 116.9% growth inhibition at the highest dose of 250μg/g diet with a GI50 （growth inhibition of 50%） value of 20.9μg/g diet. Statistical analysis showed a concentrationdependent dose response relationship toward both feeding deterrent and growth inhibitory activities. Artemisinin is found mainly in the leaves of A. annua and not in the stems, which are typically discarded as waste. Therefore identification of scopoletin in stems of A. annua may be important as a source of this material for pest control.

关键词： Artemisia annua feeding deterrent growth inhibitor scopoletin Spilartiaobliqua

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Use of a Bioaugmented Organic Soil Amendment in Combination with Gypsum for Withania somnifera Growth on Sodic Soil

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Pedosphere 2016年第3期26卷 299-309页

作者： Manjul GUPTA Pankaj Kumar SRIVASTAVA shikha Abhishek NIRANJAN Shri Krishna TEWARI CSIR National Botanical Research Institute Rana Pratap Marg Lucknow 226001 (India) School of Environmental Sciences Babasaheb Bhimrao Ambedkar University Vidya Vihar Rae Bareilly Road Lucknow 226025 (India)

Limited availability of organic matter is a problem to sustain crop growth on sodic soil. Organic soil amendments are a costeffective source of nutrients to enhance crop growth. A field study was conducted to evaluate the effect of an organic soil amendment bioaugmented with plant growth-promoting fungi(SF_(OA) ) in combination with gypsum on soil properties and growth and yield attributes of Withania somnifera, one of the most valuable crops of the traditional medicinal system in the world, on a sodic soil at the Aurawan Research Farm of CSIR-National Botanical Research Institute, Lucknow, India. The SF_(OA) used was prepared by pre-enriching farm waste vermicompost with plant growth-promoting fungi before mixing with pressmud and Azadirachta indica seed cake. The application of SF_(OA) at 10 Mg ha^(-1)after gypsum(25.0 Mg ha^(-1)) treatment significantly(P < 0.05) increased root length(by 96%) and biomass(by 125%) of Withania plants compared to the control without SF_(OA) and gypsum. Similarly, the highest withanolide contents were observed in leaves and roots of Withania plants under 10 Mg ha^(-1)SF_(OA) and gypsum. Combined application of SF_(OA) and gypsum also improved physical, chemical and enzymatic properties of the soil, with the soil bulk density decreasing by 25%, water-holding capacity increasing by 121%, total organic C increasing by 90%, p H decreasing by 17% and alkaline phosphatase, β-glucosidase, dehydrogenase and cellulase activities increasing by 54%, 128%, 81% and 96%, respectively, compared to those of the control. These showed that application of the SF_(OA) tested in this study might reclaim sodic soil and further support Withania cultivation and results were better when the SF_(OA) was applied after gypsum treatment.

关键词： medicinal crop plant growth-promoting fungi soil property soil reclamation vermicompost

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Cancer chemotherapy with novel bioactive natural products

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Journal of Chinese Pharmaceutical Sciences 2022年第8期31卷 589-607页

作者： Neha Dangi shikha Sharma Department of Pharmaceutical Chemistry Lords UniversityChikaniAlwar-301028RajasthanIndia

Cancer is the primary cause of death on a global *** year,8.8 million people die because lung,prostate gland,colorectal,and hepatic cancers are more common in *** contrast,mammary glands,large intestine,bronchi,cervix,and abdomen cancers are more common in *** Food and Drug Administration of the United States of America has approved plant-based medications for cancer *** include taxanes,such as paclitaxel,and vinca alkaloids,such as vincristine and ***,developing new anti-cancer medicine to treat this disease will necessitate a diverse range of bioactive *** than 30 natural compounds derived from plants have been identified,with clinical trials currently *** to a literature review of numerous publications and manuals,novel medicinal compounds derived from bioactive sources have been confirmed scientifically efficacious against many kinds of cancer *** current study focused on new therapeutic compounds extracted from the plants indicated to have antineoplastic effects in clinical *** most significant discoveries of these active new medicinal substances were also summarized and discussed.

关键词： Alkaloids Glycosides Antitumar Anticancer Terpenoids

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