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Mesenchymal stem cells-derived extracellular vesicles as‘natural’drug delivery system for tissue regeneration

BIOCELL 2022年第4期46卷 899-902页

作者： KENJI TSUJI shinji kitamura JUN WADA Department of Nephrology RheumatologyEndocrinology and MetabolismOkayama University Academic Field of MedicineDentistry and Pharmaceutical SciencesOkayama700-8558Japan

Mesenchymal stem cells(MSCs)have abilities to mediate tissue protection through mechanisms of antiapoptosis,anti-oxidative stress and anti-fibrosis as well as tissue regeneration through mechanisms of cell proliferation,differentiation and angiogenesis.These effects by MSCs are mediated by a variety of factors,including growth factors,cytokines and extracellular vesicles(EVs).Among these factors,EVs,containing proteins,mRNA and microRNAs(miRNA),may carry their contents into distant tissues with high stability.Therefore,the treatment with MSC-derived EVs may be promising as‘natural’drug delivery systems(DDS).Especially,the treatment of MSCderived EVs with the manipulation of specific miRNAs expression has been reported to be beneficial under a variety of diseases and tissue injuries.The overexpression of specific miRNAs in the EVs might be through pre-loading method using the gene editing system by plasmid vector or post-loading method to load miRNA mimics into EVs by electroporation or calcium chloride-mediated transfection.Despite current several challenges for clinical use,it should open the next era of regenerative medicine for a variety of diseases.In this article,we highlight the therapeutic potential of MSC-derived EVs as‘natural’DDS and current challenges.

关键词： Mesenchymal stem cells Extracellular vesicles Drug delivery system microRNA Regeneration

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Optimal Design of Electromagnetic Acoustic Transducer

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Journal of Energy and Power Engineering 2012年第4期6卷 561-569页

作者： Eiji Matsumoto shinji kitamura Jun Abe Department of Energy Conversion Science Kyoto University Kyoto 606-8501 Japan Kyoto Research ＆ Development Center Mitsubishi Motors Corporation Kyoto 616-8501 Japan Energy Technology Laboratories Osaka Gas Osaka 554-0051 Japan

This paper considers the design of EMAT (Electro-Magnetic Acoustic Transducer) based on numerical simulation. The EMAT consists of an exiting coil and two permanent magnets, which transmits the ultrasonic wave by the Lorentz force between the eddy current and the static magnetic field by the magnets. From the experimental result on self-prepared EMATs, the intensity and the directivity of the transmitted wave depend on the widths of the coil and the magnets. By means of EEM analysis the authors attempt to determine the optimal values of the above widths such that both the intensity and the directivity achieve the maximum or allowable performance.

关键词：电磁声换能器优化设计永久磁铁电磁超声超声波发射模拟设计洛仑兹力静态磁场

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Comparative studies of serum-free media and detection techniques for <i>in vitro</i>drug sensitivity assessment of <i>Plasmodium falciparum</i>

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Open Journal of Clinical Diagnostics 2013年第3期3卷 115-121页

作者： Bethel Kwansa-Bentum shinji Izumiyama Kei kitamura Kazushige Obata-Ninomiya Nobuo Ohta Hiroko Asahi Department of Animal Biology and Conservation Science Faculty of Science University of Ghana Accra Ghana Department of Environmental Parasitology Tokyo Medical and Dental University Tokyo Japan Department of Immune Regulation Tokyo Medical and Dental University Tokyo Japan Department of Parasitology National Institute of Infectious Diseases Tokyo Japan

Malaria continues to be a devastating disease. In a previous study, we formulated a chemically defined culture medium that is able to sustain the complete intraerythrocytic growth of Plasmodium falciparum. We tested the feasibility of using the medium (CDRPMI) as well as human serum-free media enriched with commercially available human-serum substitutes (GFSRPMI and ALBRPMI) to assess the drug sensitivity of P. falciparum, using chloroquine diphosphate (CQ) and dihydroartemisinin (DHART) as conventional antimalarial drugs. Growth inhibition was measured by four different methods: flow cytometry with SYBR Green I (FCM), microscopy (Giemsa method), enzymatic estimation of parasite lactate dehydrogenase (pLDH), and histidine-rich protein 2 (HRPII) determination. In drug sensitivity tests on asynchronous parasites cultured for 96 h in the presence of drugs, the dose-response curves were similar and differences in the 50% growth inhibition concentrations for the drugs, which were estimated by the four methods, were not statistically significant for the three culture media. The effect of the drugs on the growth of synchronous parasites at the ring stage was also assessed in micro-volume tests by three different methods of FCM: tracking fluorescent erythrocytes, schizont test, and merozoite test. Dose-response curves for the drugs were similar, and differences in the 50% growth inhibition concentrations were not statistically significant for CDRPMI and GFSRPMI. Thus CDRPMI as well as GFSRPMI and ALBRPMI can be similarly useful media for drug sensitivity testing of P. falciparum. The FCM, pLDH and HRPII estimations were fast and reliable detection methods, with FCM allowing schizont and merozoite tests to be performed with shorter periods of culture.

关键词： Plasmodium falciparum Chemically Defined Medium Drug Sensitivity Test Flow Cytometry

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