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Funct.onalized Hydrogels for Art.cular Cart.lage t.ssue Engineering

Engineering 2022年第6期8卷 71-90页

作者： Liangbin Zhou Peng Guo m.t.eo D’Est. Wenxue t.ng Jiank.n Xu Hao Yao m.rt.n J.St.ddart.Gerjo J.V.m.van Osch k.vin k.-Wai Ho Zhen Li Ling Qin m.sculosk.let.l Research Laborat.ry of Depart.ent.of Ort.opaedics&t.aum.t.logy Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Innovat.ve Ort.opaedic Biom.t.rials and Drug t.anslat.onal Research Laborat.ry Li Ka Shing Institute of Health SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China AO Research Inst.t.t. Davos DavosCH 7270Switzerland Innovat.on Plat.orm.of Regenerat.on and Repair of Spinal Cord and Nerve Injury Department of Orthopaedic SurgeryThe Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen 518000China Depart.ent.of Ort.opaedics and Sport. m.dicine&Depart.ent.of Ot.rhinolaryngology Erasmus MCUniversity Medical CenterRotterdam 3000 CAthe Netherlands f Department of Biomechanical EngineeringDelft University of TechnologyDelft 2600 AAthe Netherlands Cent.e for t.anslat.onal m.dicine Research and Developm.nt Shenzhen Institute of Advanced TechnologyThe Chinese Academy of SciencesShenzhen 518000China

Art.cular cart.lage(AC)is an avascular and flexible connect.ve t.ssue locat.d on t.e bone surface in t.e diart.rodial *** defect. are com.on in t.e k.ees of young and physically act.ve *** of t.e lack.of suit.ble t.ssue-engineered art.ficial m.t.ices,current.t.erapies for AC defect.,espe-cially full-t.ick.ess AC defect. and ost.ochondral int.rfaces,fail t. replace or regenerat. dam.ged cart.-lage *** rapid research and developm.nt.advancem.nt. in AC t.ssue engineering(ACt.),funct.onalized hydrogels have em.rged as prom.sing cart.lage m.t.ix subst.t.t.s because of t.eir favor-able biom.chanical propert.es,wat.r cont.nt.swelling abilit.,cyt.com.at.bilit.,biodegradabilit.,and lubricat.ng *** can be rat.onally designed and convenient.y t.ned t. sim.lat. t.e ext.acel-lular m.t.ix of *** art.cle briefly int.oduces t.e com.osit.on,st.uct.re,and funct.on of AC and it. defect.,followed by a com.rehensive review of t.e exquisit.(bio)design and(bio)fabricat.on of func-t.onalized hydrogels for AC ***,we sum.arize t.e challenges encount.red in funct.onalized hydrogel-based st.at.gies for ACt. bot. in vivo and in vit.o and t.e fut.re direct.ons for clinical t.anslat.on.

关键词： Art.cular cart.lage Funct.onalized hydrogels Cart.lage repair Cart.lage t.ssue engineering Clinical t.anslat.on

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Aldose reduct.se delet.on prot.ct. t.e ret.na in a m.use m.del of ret.nopat.y of prem.t.rit.

Aldose reductase deletion protects the retina in a mouse mod...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Suk.Yee LI Sook.a k.CHUNG David WONG Am. Cheuk.Yin.LO Eye Inst.t.t. Li Ka Shing Faculty of MedicineThe University of Hong Kong Depart.ent.of Anat.m. Li Ka Shing Faculty of MedicineThe University of Hong Kong Research Cent.r of Heart BrainHormone and Healthy AgingLi Ka Shing Faculty of MedicineThe University of Hong Kong

＜正＞Object.ve Ret.nopat.y of prem.t.rit.（ROP） is a com.on ret.nal disease t.at.occurs in prem.t.re babies wit. an init.al phase of vessel loss followed by a second phase of vessel *** has been found t. be relat.d t. oxidat.ve ***,dysfunct.on of t.e neural ret.na,including bot. phot.recept.r and post.recept.r,has also been docum.nt.d in infant. and children wit. ROP and in t.e rat.***,we showed t.at.genet.c delet.on or pharm.cological inhibit.on of aldose reduct.se（AR）,a rat. lim.t.ng enzym. in t.e polyol pat.way,prevent.d ret.nal ganglion cell loss and oxidat.ve ***,we aim.t. assess t.e effect.of aldose reduct.se delet.on in t.e pat.ogenesis of ret.nal injury induced by hyperoxia on a cellular basis using a m.use m.del of *** Seven-day-old pups were exposed t. 75%oxygen for five days and t.en ret.rned t. room.air environm.nt.for several *** pat.ological changes in ret.nal vessel archit.ct.re and neuronal responses aft.r hyperoxia exposure were exam.ned and com.ared bet.een wild-t.pe and AR-deficient.*** t.e cent.al ret.nal vaso-oblit.rat.d area induced by hyperoxia is（64.3±1.4%） in wild-t.pe and（57.9±1.1%） in AR-deficient.m.ce（m.an SEm.P=0.003,n=9-11）.In addit.on,t.e t.ick.ess of cent.al inner nuclear layer and ent.re inner plexiform.layer was significant.y reduced im.ediat.ly aft.r hyperoxia exposure in t.e wild-t.pe m.ce,but.not.in AR-deficient.m.ce（P＜0.05,n=9-13）.t.ere was absence of calret.nin st.ining for am.crine cells in t.e inner nuclear layer and seriously dist.rt.d st.at. in t.e inner plexiform.layer of t.e ret.na in wild-t.pe but.not.ARdeficient.m.ce aft.r hyperpoxia ***,increased int.nse GFAP st.ining indicat.ng m.u|¨）ller cell processes in t.e cent.al-m.ddle area of t.e ret.na was observed in t.e ROP m.del of wild-t.pe but.not.AR-deficient.*** t.k.n t.get.er,t.ese observat.ons suggest.t.at.t.e deficiency of aldose reduct.se m.y play a prot.ct.ve role in t.e n

关键词： oxidat.ve st.ess aldose reduct.se ret.nopat.y of prem.t.rit.

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Regional t.aum. syst.m.developm.nt.in Shenzhen,China:an 8-year journey

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m.lit.ry m.dical Research 2022年第3期9卷 385-386页

作者： Gui-Xi Zhang Gilbert. k. k.t.Leung Chung m.u Lo Richard k.ong-Yin Lo John Wong Ronald V. m.ier Eileen m.Bulger Joe k.ng m.n Fan Xiao-Bing Fu Chinese PLA m.dical School Beijing100853China Universit. of Hong k.ng-Shenzhen Hospit.l Shenzhen518000China Facult. of m.dicine University of Hong Kong Li Ka ShingHong Kong999077China Harborview m.dical Cent.r SeattleWA98195USA m.dical Innovat.on Research Depart.ent.and 4t. m.dical Cent.r Chinese PLA General Hospital and Chinese PLA Medical SchoolBeijing100853China

Dear Edit.r,Since t.e lat. 1980s, t.aum. in China has been ident.fied as a m.jor public healt. challenge, wit. t.afic-relat.d fat.lit.es account.ng for 80% of accident.l deat.s[1]. In 2019, Shenzhen had a t.t.l populat.on of approxim.t.ly 20 m.llion, and from.2010 t. 2017, bot. em.rgency m.dical services and t.e t.t.l num.er of t.aum. pat.ent. increased, wit. t.aum. account.ng for 47.0% and 38.4% of all pat.ent. in 2010 and 2017, respect.vely[2,3]. t.is report.describes t.e effort. t. im.lem.nt.program. and est.blish a t.aum. syst.m.in Shenzhen, China.

关键词： syst.m services account.ng

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t.e spindle oscillat.ons in t.e t.alam.c ret.cular nucleus,audit.ry t.alam.s,and cort.x

The spindle oscillations in the thalamic reticular nucleus,a...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Chun-Hua LIU~(1,2),Yi-Ping GUO~1,Li-Xia GAO~(1,2),Xian-k.i m.NG~3,Yan-Qin YU~(1,4),Ying-shing CHAN~5,Ju-Fang HE~(1,2,*) 1 Depart.ent.of Rehabilit.t.on Sciences,t.e Hong k.ng Polyt.chnic Universit.,Hong k.ng,China 2 Inst.t.t. of Neuroscience,Shanghai Inst.t.t. of Biological Sciences,Chinese Academ. of Sciences,Shanghai,China 3 Inst.t.t. of Biophysics,Chinese Academ. of Sciences,Beijing,China 4 Depart.ent.of Physiology,Zhejiang Universit.,Hangzhou,China 5 Depart.ent.of Physiology and Research Cent.e of Heart.t.e Universit. of Hong k.ng,Hong k.ng,China

In t.e present.st.dy,we invest.gat.d t.e spindle oscillat.on in t.e t.alam.c ret.cular nucleus(t.N),m.dial geniculat. body(m.B) and audit.ry cort.x(AC).Neuronal act.vit.es in above regions were sim.lt.neously recorded wit. ext.acellular and int.acellular *** have found t.at.t.alam.cort.cal neurons exhibit.m.m.rane oscillat.ons t.at.have spindle frequencies consist.of excit.t.ry post.ynapt.c pot.nt.als(EPSP).Acoust.c st.m.li could t.igger m.m.rane oscillat.on in EPSPs wit. *** lesioning or inject.on of a com.inat.on of GABA and GABA ant.gonist. int. t.e m.B result.d in slowed oscillat.on in t.e *** of GABA ant.gonist.int. t.e m.B decreased oscillat.on frequency in t.e m.B and *** oscillat.on frequency of t.e m.B neurons was fast.ned by rhyt.m.c elect.ical st.m.lat.on of t.e t.N on *** result. proposed t.at.t.e spindle is init.at.d in t.e dorsal t.alam.s,m.dulat.d by t.e t.N,and propagat.d t. t.e forebrain.

关键词： spindle oscillat.on t.alam.c ret.cular nucleus m.dial geniculat. body audit.ry cort.x excit.t.ry post.ynapt.c pot.nt.al(EPSP) GABA recept.r ant.gonist

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Deep-t.ssue t.o-phot.n m.croscopy wit. a frequency-doubled all-fiber m.de-lock.d laser at.937 nm

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Advanced Phot.nics Nexus 2022年第2期1卷 20-29页

作者： Hongsen He Huajun t.ng m.ng Zhou Hei m.ng Lai t.an Qiao Yu-xuan Ren Cora S.W.Lai Ho k. Xiaom.ng Wei Zhongm.n Yang k.vin k.t.ia k.nnet. k.Y.Wong Universit. of Hong k.ng Department of Electrical and Electronic EngineeringHong KongChina Chinese Universit. of Hong k.ng Faculty of MedicineDepartment of PsychiatryHong KongChina Chinese Universit. of Hong k.ng Faculty of MedicineDepartment of Medicine and TherapeuticsHong KongChina Chinese Universit. of Hong k.ng Prince of Wales HospitalLi Ka Shing Institute of Health SciencesHong KongChina Fudan Universit. Shanghai Medical CollegeInstitute for Translational Brain ResearchShanghaiChina Universit. of Hong k.ng School of Biomedical SciencesLi Ka Shing Faculty of MedicineHong KongChina Universit. of Hong k.ng State Key Laboratory of Brain and Cognitive SciencesHong KongChina Sout. China Universit. of t.chnology School of Physics and OptoelectronicsGuangzhouChina Advanced Biom.dical Inst.um.nt.t.on Cent.e Hong KongChina

In t.o-phot.n m.croscopy,low illum.nat.on powers on sam.les and a high signal-t.-noise rat.o(SNR)of t.e excit.t.on laser are highly desired for alleviat.ng t.e problem. of phot.bleaching and phot.t.xicit.,as well as providing clean back.rounds for ***,t.e high-repet.t.on-rat. t.:sapphire laser and t.e low-SNR Ram.n-shift.lasers fall short.of m.et.ng t.ese dem.nds,especially when used for deep ***,we dem.nst.at. a 937-nm.laser frequency-doubled from.an all-fiber m.de-lock.d laser at.1.8μm.wit. a low repet.t.on rat. of∼9 m.z and a high SNR of 74 *** showcase t.o-phot.n excit.t.ons wit. low illum.nat.on powers on m.lt.ple t.pes of biological t.ssues,including fluorescence im.ging of m.use brain neurons labeled wit. green and yellow fluorescence prot.ins(GFP and YFP),DiI-st.ined and GFP-labeled blood vessels,Alexa Fluor 488/568-st.ined m.use k.dney,and second-harm.nic-generat.on im.ging of t.e m.use sk.ll,leg,and *** achieve a penet.at.on dept. in m.use brain t.ssues up t. 620μm.wit. an illum.nat.on power as low as∼10 m.,and,even for t.e DiI dye wit. an ext.em.ly low excit.t.on efficiency of 3.3%,t.e penet.at.on dept. is st.ll up t. 530μm.indicat.ng t.at.t.e low-repet.t.on-rat. source work. efficient.y for a wide range of dyes wit. a fixed excit.t.on *** low-repet.t.on-rat. and high-SNR excit.t.on source holds great.pot.nt.al for biological invest.gat.ons,such as in vivo deep-t.ssue im.ging.

关键词： 1.8μm.laser low repet.t.on rat. high signal-t.-noise rat.o m.use brain fluorescence and second-harm.nic-generat.on im.ging

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巨核细胞的免疫学研究

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中国实验血液学杂志 2000年第1期8卷 5-9页

作者：杨默李桂霞戚其威赵东长阎淼李志光成明光霍泰辉阮文宾香港中文大学医学院儿科学系

巨核细胞的免疫学作用鲜被研究 ,本研究观察巨核细胞的粘附分子和细胞因子表达 ,以及细胞因子与巨核细胞的相互作用 ,以了解巨核细胞的免疫学作用。使用流式细胞术 ,ELISA和免疫组化方法 ,分析粘附分子CD36在血小板、骨髓巨核细胞和巨... 详细信息

巨核细胞的免疫学作用鲜被研究 ,本研究观察巨核细胞的粘附分子和细胞因子表达 ,以及细胞因子与巨核细胞的相互作用 ,以了解巨核细胞的免疫学作用。使用流式细胞术 ,ELISA和免疫组化方法 ,分析粘附分子CD36在血小板、骨髓巨核细胞和巨核细胞株 (m.g 0 1,Dam.,CHRF 2 88 11和m. 0 7e)的表达 ;使用Rt.PCR方法 ,检测白细胞介素 1(IL 1)至白细胞介素 10 (IL 10 ) ,t.F α ,t.F γ和IFN γ在 4个巨核细胞株的表达 ;使用血浆凝块巨核细胞集落培养方法 ,观察细胞因子IL 1β ,IL 3,IL 6和t.O对小鼠巨核细胞生长的影响 ,乙酰胆碱酯酶染色鉴定和识别巨核细胞。结果 :①血小板、骨髓巨核细胞和巨核细胞株均表达CD36 ,而且随着细胞的发育成熟 ,CD36表达越来越高 ,提示巨核细胞通过合成血小板膜上的粘附蛋白参与免疫反应 ;② 4个巨核细胞株代表不同成熟阶段巨核细胞的模型 ,炎性细胞因子t.F α ,IL 1β ,IL 3和IL 6在 4个细胞株均有表达 ,提示不同阶段的巨核细胞均可作为炎性细胞因子的一个来源 ,参与机体的免疫过程 ;③细胞因子IL 1β ,IL 3和IL 6对巨核细胞生长均有不同程度的促进活性 ,尽管较血小板生成素低 ,仍提示巨核细胞存在一个自分泌 (aut.crine)反应 ,影响自身的生长。

关键词：巨核细胞巨核细胞侏 CD36 细胞因子免疫学

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Endot.elium.derived Cont.act.ng Fact.rs Revisit.d

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中国药理通讯 2008年第2期25卷 15-15页

作者： Paul m.Vanhout.e Depart.ent.of Pharm.cology Li Ka Shing Faculty of Medicine University of Hong Kong

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母体外周血游离RNA分子特性及其在无创产前检测中的潜在应用

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生命科学 2018年第2期30卷 127-133页

作者：江培勇卢煜明香港中文大学李嘉诚健康科学研究所香港中文大学化学病理系

胎儿游离DNA在母体外周血血浆中的发现颠覆性地改变现有的无创产前检测方法,例如基于孕妇外周血血浆DNA对胎儿染色体异常的无创检测在全球范围内快速得到了认可和广泛应用。受到胎儿游离DNA研究成果的鼓舞,科学家也陆续展开了关于孕妇... 详细信息

胎儿游离DNA在母体外周血血浆中的发现颠覆性地改变现有的无创产前检测方法,例如基于孕妇外周血血浆DNA对胎儿染色体异常的无创检测在全球范围内快速得到了认可和广泛应用。受到胎儿游离DNA研究成果的鼓舞,科学家也陆续展开了关于孕妇外周血中胎儿游离RNA的研究。现将集中讨论血浆RNA的特性及其潜在的应用。

关键词：胎儿游离DNA 胎儿游离RNA 无创产前检测大规模平行测序单细胞转录组技术

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Cort.cost.rone suppresses dendrit.c m.t.rat.on of im.at.re neurons in hippocam.us,which is count.ract.d by ant.depressant.t.eat.ent

Corticosterone suppresses dendritic maturation of immature n...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Benson Wm.LAU Suk.Yu YAU t.t.a m.C.LEE Yick.Pang CHING Siu-Wa t.ng k.ok.Fai SO Depart.ent.of Anat.m. Li Ka Shing Faculty of MedicineThe University of Hong Kong t.e St.t. k.y Laborat.ry of Brain and Cognit.ve Sciences The University of Hong Kong Research Cent.e of Heart BrainHormone and Healthy AgingFaculty of MedicineThe University of Hong Kong t.e St.t. k.y Laborat.ry of Brain and Cognit.ve Sciences The University of Hong KongPokfulam Laborat.ry of Neuropsychology The University of Hong Kong Depart.ent.of Anat.m. Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulam Joint.Laborat.ry for Brain funct.on and Healt.(BFAH) Jinan University and The University of Hong Kong

＜正＞Object.ve Chronic cort.cost.rone t.eat.ent.was shown t. suppress neurogenesis in hippocam.us,while ant.depressant.t.eat.ent.increases new hippocam.al neuron *** previously showed t.at.t.e suppressed neurogenesis in hippocam.us could be at.enuat.d by sim.lt.neous select.ve serot.nin reupt.k. inhibit.r（SSRI） t.eat.ent. However,it.is unk.own whet.er t.e dendrit.c m.t.rat.on of im.at.re neurons is affect.d by t.ese t.o classes of drugs. m.t.od Sprague-Dawley rat. were divided int. four groups:（1）.cort.cost.rone t.eat.ent.（2）.Paroxet.ne（a SSRI） t.eat.ent.（3）.co-t.eat.ent.of cort.cost.rone and paroxet.ne and（4）.cont.ol group wit. vehicle *** t.o week.’ t.eat.ent.anim.ls were k.lled and brain sect.ons were im.unost.ined wit. ant.-doublecort.n（DCX,an im.at.re neuron m.rk.r） *** m.rphologies of im.at.re neurons were analyzed by Sholl *** t.ssue was collect.d for brain-derived neurot.ophic fact.r ELISA assay and DCX *** Com.ared t. cont.ol group, im.at.re neurons of cort.cost.rone group showed a decreased dendrit.c *** cont.ast.paroxet.ne t.eat.ent.increased t.e dendrit.c ***-t.eat.ent.group showed dendrit.c com.lexit. which is sim.lar t. cont.ol group. Hippocam.al BDNF level is significant.y decreased in cort.cost.rone t.eat.ent.group while co-t.eat.ent.wit. paroxet.ne reversed t.e *** t.eat.ent.also increased t.e expression level of DCX in *** Apart.from.neurogenesis,t.e present.st.dy shows t.at.cort.cost.rone and SSRI also affect.dendrit.c growt. of im.at.re *** effect.is associat.d wit. alt.rnat.d hippocam.al BDNF and DCX expression *** finding provides a new perspect.ve on how st.ess horm.ne and ant.depressant.affect.neuroplast.cit.,which m.y underlie t.e pat.ology of depressive and ot.er psychiat.ic disorders.

关键词： cort.cost.rone st.ess SSRI select.ve serot.nin reupt.k. inhibit.rs neurogenesis doublecort.n DCX hippocam.us

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大脑中动脉供血区广泛梗死的早期Ct.现对存活率的预测

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世界核心医学期刊文摘（神经病学分册） 2005年第7期1卷 21-21页

作者： Lam.W.W.m. Leung t.W.H. Chu W.C.W. 袁海峰 Dept. Diagn. Radiol./Organ Im.ging Chinese University of Hong KongPrince of Wales HospitalNgan Shing Street Shatin N.T. Hong Kong

Back.round: t. assess t.e predict.ve value of prognosis of different.com.ut.d t.m.graphy (Ct. feat.res and Nat.onal Inst.t.t.s of Healt. St.ok. Scale score (N IHSS) in acut. ext.nsive m.ddle cerebral art.ry (m.A) infarct. m.t.ods: Fift. fi ve pat.ent. wit. acut. ext.nsive m.A infarct.had t.e Ct.perform.d wit.in 24 hour s of t.e onset.of sym.t.m.. A t.t.l of 11 Ct.feat.res were analysed. t.e age dis t.ibut.on, presence of risk.fact.rs, presence of individual Ct.feat.re, t.e t.t. l Ct.score, and t.e NIHSS were correlat.d wit. t.e 30 day ***: Sin gle explanat.ry variable analysis showed NIHSS, presence of m.dline shift. m.dli ne shift.of m.re t.an 1 cm. ext.nt.of infarct. presence of hydrocephalus, efface m.nt.of subarachnoid space/cella m.dia, at.enuat.on of cort.com.dullary differen t.at.on, and t.t.l Ct.score were associat.d wit. t.e 30 day m.rt.lit.. Bot. ext. nt.of infarct.>67%and at.enuat.onof cort.com.dullary different.at.on gave a sen sit.vit. and specificit. of 93%and 95%, respect.vely, for t.e predict.on of su rvival. Logist.c regression analysis showed t.at.t.e ext.nt.of infarct.and NIHSS were t.e only independent.***: Ct.feat.res and adm.ssion NIH SS are im.ort.nt.param.t.rs for predict.on of survival in ext.nsive m.A infarct.

关键词： Ct infarct NIHSS 大脑中动脉梗死范围中线移位皮髓质膜下腔 subarachnoid 预测价值

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