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Expression of miR-125b in the new,highly invasive glioma stem cell and progenitor cell line SU3

Chinese Journal of Cancer 2012年第4期31卷 207-214页

作者： yi Wan Xi-Feng Fei Zhi-Min Wang Dong-yi Jiang Han-Chun Chen Jian Yang Lei shi Qiang Huang Suzhou Kowloon Hospital Shanghai Jiaotong University School of MedicineSuzhouJiangsu 215021P.R.China The First People's Hospital of Kunshan Affliated to Jiangsu University KunshanJiangsu 215300P.R.China The Second Hospital Affliated to Soochow University SuzhouJiangsu 215004P.R.China

MicroRNA (miR)-125b has been shown to play a potential role in the development of glioma stem ***,the relationship between miRNA and glioma stem cells is still *** study was designed to elucidate this potential *** established a highly invasive glioma stem cell and progenitor (GSCP) cell line ***3 cell suspensions were injected into nude mice brains in situ,and the invasiveness of graft tumors was analyzed using hematoxylin and eosin staining as well as ***-time polymerase chain reaction (PCR) was used to measure the expression levels of miR-125b in SU3 and other *** vitro,SU3 cells expressed CD133 and nestin as well as differentiation markers glial fibrillary acidic protein (GFAP) and β-tubulin III,which were consistent with the characteristics of glioma stem *** assays indicated that the migration ability of SU3 cells was stronger than that of U251 stem cells (U251s).In vivo,SU3 cells invaded into each part of the mouse brain from the caudate nucleus in a diffuse pattern and highly expressed invasive and proliferative cell markers matrix metalloprotease 2 (MMP2),MMP9,and ***-time PCR results revealed that the levels of miR-125b and MMP9 were significantly higher in SU3 and SU2,also a highly invasive GSCP cell line we established before,than in *** expression of miR-125b both in newly established GSCPs,SU3,and long-term cultured GSCPs,SU2 suggests that miR-125b exhibits oncogene-like *** behavior should be considered in further studies of miR-125b in cancer stem ***,MMP9,which plays a role in cancer stem cell invasion,may be a target gene of miR-125b.

关键词：脑胶质瘤干细胞细胞系入侵性胶质纤维酸性蛋白实时PCR 基质金属蛋白酶聚合酶链反应

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Leonurine: From Gynecologic Medicine to Pleiotropic Agent

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Chinese Journal of Integrative Medicine 2020年第2期26卷 152-160页

作者： LI Yun-yun LIN yi-kong LIU xin-hua WANG Li YU Min LI Da-jin ZHU yi-zhun DU Mei-rong NHC Key Lab of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research) Hospital of Obstetrics and Gyn ecologyFuda n Un iversity Sha nghai Medical CollegeShanghai(200011)China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai(200011)China Shanghai Key Laboratory of Bioactive Small Molecules Department of PharmacologySchool of PharmacyFudan UniversityShanghai(201203)China State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy Macao Un iversity of Science and Tech no logyMacao SARChina

Leonurus japonicus Houtt,a Chinese traditional herbal medicine,has been widely used to cure gynecological diseases,such as incomplete abortion and ***,a major active alkaloid compound only be found in Leonurus japonicus Houtt,has been successfully extracted and *** evidence has shown that leonurine can regulate a variety of pathologic processes including oxidative stress,inflammation,fibrosis,apoptosis,and multiple metabolic ***,we have reviewed the pharmacological actions and biological functions of leonurine,with a focus on the role of leonurine in the amelioration of various pathological *** into the related signaling pathways and molecular mechanisms have strengthened our understanding on the function of leonurine in the alleviation of multiple pathological *** summary of the existing researches should help direct future research into the basic science and clinical applications in related diseases.

关键词： leonurine Leonurus japonicus Houtt Chinese medicine oxidative stress inflammation fibrosis clinical application

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多粘类芽胞杆菌新菌株X-11及其对番茄和水稻的促生效应

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生物技术通报 2024年第9期40卷 249-259页

作者：刘文志贺丹李鹏傅应林张译心温华杰于文清上饶师范学院生命科学学院上饶334001 上饶师范学院生态环境与绿色发展研究中心赣东北特色植物资源保育与生态修复重点实验室上饶334001 黑龙江省农垦科学院哈尔滨150038

【目的】从小麦(Triticum aestivum L)根际筛选到了一株细菌菌株(编号X-11),为该菌株在植物促生上应用提供依据。【方法】结合形态特征、生理生化特性、16S rDNA序列及基因组测序对其进行了鉴定,并测定其对番茄(Solanum lycopersicum)... 详细信息

【目的】从小麦(Triticum aestivum L)根际筛选到了一株细菌菌株(编号X-11),为该菌株在植物促生上应用提供依据。【方法】结合形态特征、生理生化特性、16S rDNA序列及基因组测序对其进行了鉴定,并测定其对番茄(Solanum lycopersicum)和水稻(Oryza sativa)的促生效应。【结果】该菌株菌落圆形、半透明、凸起、黏稠;菌体杆状,形成芽胞,革兰氏阴性;具有固氮,还原硝酸盐,产吲哚、分泌铁载体及分解有机磷的功能。基于16S rDNA序列比对将X-11菌株归类为多粘类芽胞杆菌种群,基于非冗余蛋白数据库的基因比对表明其与最近缘菌株基因相似率55.7%,证明其为一个新菌株。X-11菌液浸种显著增加了番茄发芽率和胚根伸长度,灌根(R)及灌根结合叶面喷施(R+L)显著提高了番茄叶片中过氧化物酶(POD)活性,R+L处理使超氧化物歧化酶(SOD)活性提高了34.58%。水稻1叶1心期施用X-11菌液,根长增加了6.8%;立针期或1叶1心期施用X-11菌液,使秧苗SOD活性分别提高了90.4%和51.8%。【结论】X-11被鉴定为多粘类芽胞杆菌新菌株,是多种功能的植物根际促生菌,在植物促生方面具有开发应用潜力。

关键词：多粘类芽胞杆菌植物根际促生菌番茄水稻

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Advances in spatiotemporal graph neural network prediction research

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International Journal of Digital Earth 2023年第1期16卷 2034-2066页

作者： Jianghong Zhao yi Wang xintong Dou xin Wang Ming Guo Ruiju Zhang Haimeng Li School of Geomatics and Urban Spatial Informatics Beijing University of Civil Engineering and ArchitectureBeijingPeople’s Republic of China Beijing Key Laboratory for Architectural Heritage Fine Reconstruction&Health Monitoring BeijingPeople’s Republic of China Key Laboratory for Urban Spatial Information of the Ministry of Natural Resources BeijingPeople’s Republic of China School of Earth and Space Sciences Peking UniversityBeijingPeople’s Republic of China School of Humanities Beijing University of Civil Engineering and ArchitectureBeijingPeople’s Republic of China

Being a kind of non-Euclidean data,spatiotemporal graph data exists everywhere from trafficflow,air quality index to crime case,*** the raster data,the irregular and disordered characteristics of spatiotemporal graph data have attracted the research interest of scholars,with the prediction of spatiotemporal graph data being one of the research hot *** emergence of spatiotemporal graph neural networks(ST-GNNs)provides a new insight for solving the problem of obtaining spatial correlation for spatiotemporal graph data prediction while achieving state-of-the-art *** this paper,comprehensive survey of research on ST-GNNs prediction domain isa presented,where the background of ST-GNNs is introduced before the computational paradigm of ST-GNN is thoroughly *** the perspective of model construction,59 well-known models in recent years are classified and *** of these models are further analyzed in terms of performance and ***,the categories and applicationfields of spatiotemporal graph data are summarized,providing a clear idea of technology selection for different ***,the evolution history and future direction of ST-GNNs are also summarized,to facilitate future researchers to timely understand the current state of prediction research by ST-GNNs.

关键词： Spatiotemporal graph neural network prediction models spatiotemporal graph data

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Comparisons of Condylar Movements with the Functional Occlusal Clutch and Tray Clutch Recording Methods in CADIAX~ system

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International Journal of Oral Science 2010年第4期2卷 208-214页

作者： Bi-jie Han Hong Kang Lai-kui Liu xin-zhu yi Xiao-qing Li Department of Oral Anatomy and Physiology West China College of Stomatology Sichuan University Chengdu China Institute of Stomatology Lanzhou University Lanzhou China College of Stomatology Nanjing Medical Science University Nanjing China

Aim The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. Methodology Ten subjects （6 women,4 men; mean age 25.4 years） participated in the study. The mandibular move-ment,sagittal condylar inclination angle,and transversal condylar inclination angle of each subject were recorded with the CADIAX？ using the two clutches,respectively. The characteristics of the tracings of the protrusion,opening,and mediotrusion were analyzed with the t-test statistics at α = 0.05 level. The Kappa values were calculated for an assessment of the congruence of the tracings. Results The results showed that the contour,direction,and dimension of the tracings in the two clutches were approxi-mately same,but the tracings determined by the functional occlusal clutch were more regular and congruent. In the group segment recorded with the tray clutch,opening/ closing paths of one subject showed crossed and time curves of three subjects appeared peak-like changes of velocity,but none were statistically different （P0.05）. Conclusion The research suggests that the functional occlusal clutch should be preferred in the evaluation of the mandibular function,as the tracings with the tray clutch are more likely to produce false positive results.

关键词： CADIAX temporomandibular joint mandibular movement functional clutch occlusion

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Selective Reduction of 7-Demethylsinomenine

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Chemical Research in Chinese Universities 2008年第5期24卷 557-560页

作者： LI Yu-feng BU Qing-min QIAN yi PAN yi LI Jian-xin HUANG Le-qun ZHU Hong-jun School of Science Nanjing University of Technology Nanjing 210009 P. R. China Department of Chemistry School of Chemistry and Chemical Engineering School of Medicine Nanjing University Nanjing 210093 P. R. China

Several borohydride reagents： sodium borohydride, sodium cyanoborohydride, soudium acetoxyborohydride and soudium triacetoxyborohydride were screened, respectively, for the reduction of 7-demethylsinomenine, which was an α,β-dicarbonyl compound derived from sinomenine. Highly regio-and stereo-selectivity was acquired when sodium cyanoborohydride or NaBH（OAc）3 was used. The product was structurally confirmed as 7R configuration by NMR, X-ray crystal diffraction analysis. Some preliminary discussion was also made on the mechanism of the selective reduction.

关键词： 7-Demethylsinomenine Regioselective reduction Stereoselective reduction Borohydride reagents

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Formation and Control of Sharp {100}<021> Texture in Electrical Steel

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Journal of Iron and Steel Research International 2006年第2期13卷 54-58页

作者： WANG Jun-an ZHOU Bang-xin YAO Mei-yi LI Qiang CHEN Wen-jue Institute of Materials Shanghai UniversityShanghai 200072China

The formation and control of sharp{100}〈021〉texture in electrical steels were experimentally investigated and the main influencing factors were *** steels of a base composition of Fe-3.2%Si with a certain amount of carbon and manganese were employed to obtain{100}〈021〉texture through one stage cold rolling and *** results indicated that by careful control of the amount of alloying elements and the annealing temperature,a relatively sharp{100}〈021〉texture in fairly thick sheets is *** primary recrystallization,a weak component of{100}〈021〉and strong component of(447)〈18114〉texture,which constitute∑9 coincidence site lattice boundaries with a common〈011〉axis for 39°difference,are *** special grain boundary promotes the growth of{100}〈021〉grains in addition to the lower surface energy of(100)grains during secondary recrystallization.

关键词： {100}〈021〉texture electrical steel recrystallization grain growth

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Triazole Resistance in Aspergillus fumigatus Clinical Isolates Obtained in Nanjing, China

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Chinese Medical Journal 2017年第6期130卷 665-668页

作者： Ming Zhang Chun-Lai Feng Fei Chen Qian He xin Su yi shi Department of Respiratory and Critical Care Medicine Jinling Hospital Medical School of Nanjing University Nanjing Jiangsu 210002 China Department of Respiratory Medicine The Third Affiliated Hospital of Soochow University Changzhou Jiangsu 213003 China

Background： During the past decades, the incidence of invasive aspergillosis （IA） caused by Aspergillus funtigatus has increased dramatically. The aims of this study were to investigate the susceptibility of clinical isolates of *** to triazole and the underlying cyp51A mutations in triazole-resistant A. fumigatus. Methods： A total of 126 *** clinical isolates from 126 patients with proven or probable IA were obtained from four large tertiary hospitals in Nanjing, China, between August 2012 and July 2015. The determination of minimal inhibitory concentrations （MICs） for itraconazole, voriconazole, and posaconazole was performed by broth microdilution according to the European Committee on Antimicrobial Susceptibility Testing reference method. Results： A total of 4 *** isolates （3.17%） were confinned to be itraconazole resistant, with MICs of ≥8 mg/L, and one isolate （0.8%） was confirmed to be voriconazole resistant and posaconazole resistant, with MICs of 4 mg/L and 0.5 mg/L, respectively. We found that two of the 4 isolates of triazole-resistant A. fumigatus had the L98H amino acid substitution in combination with a 34-base pair tandem repeat in the promoter region, one isolate had an M2201 mutation, and another itraconazole-resistant isolate did not have a substitution in the civp51A gene. Conclusions： This study shows that triazole-resistant *** clinical isolates are present in Nanjing, China, which is a new challenge to the clinical management of IA.

关键词： Aspergillus fumigatus cyp51A Minimal Inhibitory Concentrations TR34/L98H Triazole Resistance

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狼疮性肾炎患者血清NETs、TWEAK、外周血CD4^(+)T/CD8^(+)T比例与疾病活动度及肾脏预后的关系

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现代生物医学进展 2024年第1期24卷 194-200页

作者：钟凤梅易娟郭昕王菲夏成云川北医学院附属医院肾内科四川南充637000

目的:探讨狼疮性肾炎(LN)患者血清中性粒细胞胞外诱捕网(NETs)、肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)、外周血分化簇(CD)4^(+)T/CD8^(+)T比例与疾病活动度及肾脏预后的关系。方法:选取2021年8月~2022年8月川北医学院附属医院肾内科收治... 详细信息

目的:探讨狼疮性肾炎(LN)患者血清中性粒细胞胞外诱捕网(NETs)、肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)、外周血分化簇(CD)4^(+)T/CD8^(+)T比例与疾病活动度及肾脏预后的关系。方法:选取2021年8月~2022年8月川北医学院附属医院肾内科收治的LN患者137例(LN组),根据系统性红斑狼疮疾病活动指数(SLEDAI)-2000评分分为轻度活动组(52例)、中度活动组(45例)、重度活动组(40例)。随访1年,根据肾脏相关终点事件发生情况分为预后不良组(43例)和预后良好组(94例),另选取同期76名体检健康志愿者(对照组)。采用酶联免疫吸附法检测血清NETs、TWEAK水平,流式细胞术检测外周血CD4^(+)T/CD8^(+)T比例。Spearman相关性分析LN患者血清NETs、TWEAK和外周血CD4^(+)T/CD8^(+)T与SLEDAI-2000评分的相关性,多因素Logistic回归分析LN患者预后不良的因素,受试者工作特征曲线分析血清NETs、TWEAK和外周血CD4^(+)T/CD8^(+)T对LN患者预后不良的预测价值。结果:与对照组比较,LN组血清NETs、TWEAK水平升高,外周血CD4^(+)T/CD8^(+)T降低(P<0.05)。轻度活动组、中度活动组、重度活动组血清NETs、TWEAK依次升高,外周血CD4^(+)T/CD8^(+)T依次降低(P<0.05)。LN患者SLEDAI-2000评分与血清NETs、TWEAK呈正相关,与外周血CD4^(+)T/CD8^(+)T呈负相关(P<0.05)。慢性肾脏病分期4期、SLEDAI-2000评分升高、NETs升高、TWEAK升高为LN患者预后不良的独立危险因素,估算肾小球滤过率升高、CD4^(+)T/CD8^(+)T升高为独立保护因素(P<0.05)。血清NETs、TWEAK和外周血CD4^(+)T/CD8^(+)T联合预测LN患者预后不良的曲线下面积为0.943,大于血清NETs、TWEAK和外周血CD4^(+)T/CD8^(+)T单独预测的0.790、0.788、0.799(P<0.05)。结论:LN患者血清NETs、TWEAK水平升高,外周血CD4^(+)T/CD8^(+)T降低,与疾病活动度及肾脏预后不良密切相关,血清NETs、TWEAK联合外周血CD4^(+)T/CD8^(+)T预测LN患者肾脏预后的价值较高。

关键词：狼疮性肾炎 NETs TWEAK CD4+T/CD8+T 疾病活动度肾脏预后

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合成生物可降解塑料PBAT催化剂的研究进展

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现代化工 2024年第10期44卷 21-25页

作者：李欣蓓王明义吴华帅韩卓然王俊文太原理工大学化学工程与技术学院山西太原030024 华阳新材料科技集团有限公司山西阳泉045000

重点介绍了合成PBAT催化剂领域的研究进展,分析了不同体系钛基催化剂的优势与不足,并简述了目前合成PBAT过程中酯化与缩聚阶段的催化反应机理,提出合成PBAT催化剂的改进措施与研究方向,指出研制高效环保的催化剂是未来可生物降解聚酯行... 详细信息

重点介绍了合成PBAT催化剂领域的研究进展,分析了不同体系钛基催化剂的优势与不足,并简述了目前合成PBAT过程中酯化与缩聚阶段的催化反应机理,提出合成PBAT催化剂的改进措施与研究方向,指出研制高效环保的催化剂是未来可生物降解聚酯行业的主流发展趋势。

关键词：可生物降解聚合物 PBAT 钛基催化剂配位能力抗水解性催化机理

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