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Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer

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World Journal of Gastroenterology 2015年第5期21卷 1479-1487页

作者： Jun shi pin-Kang Wei Department of Traditional Chinese Medicine Shanghai Changzheng HospitalThe Second Military Medical University

AIM:To investigate the interaction between Xiaotan Sanjie(XTSJ) decoction and interleukin-8(IL-8) and its effect on adhesion,migration and invasion of SGC-7901 gastric cancer ***:SGC-7901 gastric cancer cells were exposed to serum containing XTSJ decoction and/orIL-8(1 ng/m L).SGC-7901 cell adhesion to fibronectin,an extracellular matrix component,was detected using the Cell Counting *** and invasion abilities of SGC-7901 cells were detected by scratch wound and Transwell chamber ***,protein(immunofluorescence and Western blot) and m RNA levels(quantitative polymerase chain reaction) of cluster of differentiation 44(CD44),a cell adhesion molecule,were measured in 72-h-cultured SGC-7901 ***:Cell adhesion was promoted by IL-8(P = 0.001),but was inhibited by XTSJ decoction(P = 0.0001).Similarly,IL-8 promoted SGC-7901 cell invasion(P = 0.003),and XTSJ decoction inhibited cell invasion(P = 0.001).IL-8 induced SGC-7901 cell migration,but this was inhibited by XTSJ ***-8 up-regulated CD44 protein(P = 0.028) and m RNA expression(P = 0.002),whereas XTSJ decoction inhibited CD44 protein expression(P = 0.0001),but not m RNA expression(P = 0.275).An interaction between XTSJ decoction and IL-8 was confirmed in the invasion(P = 0.001) and CD44 m RNA expression of SGC-7901 cells(P = 0.010),but not in cell adhesion(P = 0.051).CONCLUSION:XTSJ decoction may inhibit adhesion,migration and invasion of gastric cancer cells,which is partly associated with down-regulation of IL-8.

关键词： Xiaotan Sanjie decoction Interleukin-8 Migration A

Model Order Reduction for Coupled Dynamic Characterization of Torsional Micromirrors

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Chinese Journal of Mechanical Engineering 2011年第5期24卷 829-835页

作者： shi jian ZHANG Shuyou Department of Mechanical Engineering Zhejiang University Hangzhou 310027 China

Numerical solutions could not perform rapid system-level simulation of the behavior of micro-electro-mechanical systems（MEMS） and analytic solutions for the describing partial differential equations are only available for simple *** order reduction（MOR） can extract approximate low-order model from the original large scale *** model order reduction algorithm is based on first-order system model,however,most structure mechanical MEMS systems are naturally second-order in *** the purpose of solving the above problem,a direct second-order system model order reduction approach based on Krylov subspace projection for the coupled dynamic study of electrostatic torsional micromirrors is *** block Arnoldi process is applied to create the orthonormal vectors to construct the projection matrix,which enables the extraction of the low order model from the discretized system assembled through finite element *** transfer functions of the reduced order model and the original model are expanded to demonstrate the moment-matching property of the second-order model reduction *** torsion and bending effect are included in the finite element model,and the squeeze film damping effect is considered as *** empirical method considering relative error convergence is adopted to obtain the optimal choice of the order for the reduced model.A comparison research between the full model and the reduced model is carried *** modeling accuracy and computation efficiency of the presented second-order model reduction method are confirmed by the comparison research *** research provides references for MOR of MEMS.

关键词： model order reduction torsional micromirror moment-matching

Tensile and compressive behavior of Ti-based bulk metallic glass composites

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International Journal of Minerals,Metallurgy and Materials 2013年第6期20卷 582-588页

作者： Yong-sheng Wang Guo-jian Hao Jun-pin Lin State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing

This article focuses on the tensile and compressive characteristics of a Ti-based bulk metallic glass composite （BMGC）. It is found that the yield stress, maximum strength, and fracture strain are 1380 MPa, 1516 MPa, and 4.3% for uniaxial tension, but 1580 MPa, 4010 MPa, and 29% for uniaxial compression, respectively. The composite displays a linear ＂work hardening＂ capacity under compression; however, the ＂work softening＂ behavior is observed in the true engineering stress-strain curve upon tensile loading. The fracture surfaces of specimens also exhibit dissimilar properties under the different loadings.

关键词： metallic glass composite materials tensile testing compression testing fracture

Optimization of Two-Dimensional Gel Electrophoresis for Kenaf Leaf Proteins

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Agricultural Sciences in China 2011年第12期10卷 1842-1850页

作者： CHEN Tao QI jian-min XU jian-tang CHEN pin-pin TAO Ai-fen CHEN Fu-cheng CHEN Wei Key Laboratory for Genetics Breeding and Multiple Utilization of Crops Ministry of Education/Fujian Agriculture and Forestry University Fuzhou 350002. P.R.China

To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis （2-DE） analysis in kenaf leaf tissues, three extraction methods （trichloroacetic acid/acetone, urea/thiourea, and phenol extraction methods） were applied to the extraction of kenaf leaf protein. The results were compared in regard to protein extraction efficiency, sodiumdodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, and 2-DE gels. Furthermore, the 2-DE system was optimized for four aspects： the pH range of IPG （immobilized pH gradient） stripes, sampling methods, sample volumes, and concentration of polyacrylamide gels. The data presented showed that the phenol extraction method is the best method to perform 2-DE analysis of kenaf leaf protein. The protein extracted from phenol extraction method reached the purity of （26.40±0.859）%, showed （25.67±1.53） protein bands in one dimension SDS-PAGE gels, and （1 374±54.44） protein spots on 2-DE gels. The research also indicates that kenaf leaf protein spots were distributed mainly within the pH range of 4-8. More clear background with a better distribution effect and many protein spots could be obtained on 2-DE gels under the conditions of active rehydration loading, 24 cm IPG strips （linear pH gradient of 4-7）, 1.4 mg samples, and 12% SDS-PAGE gels.

关键词： kenaf protein extraction proteome two-dimensional gel electrophoresis

Efficient SIMD optimization for media processors

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第4期9卷 524-530页

作者： jian-peng ZHOU Ce shi Department of Information Science and Electronic Engineering Zhejiang University Hangzhou 310027 China

Single instruction multiple data （SIMD） instructions are often implemented in modem media processors. Although SIMD instructions are useful in multimedia applications, most compilers do not have good support for SIMD instructions. This paper focuses on SIMD instructions generation for media processors. We present an efficient code optimization approach that is integrated into a retargetable C compiler. SIMD instructions are generated by finding and combining the same operations in programs. Experimental results for the UltraSPARC VIS instruction set show that a speedup factor up to 2.639 is obtained.

关键词： Retargetable compiler Single instruction multiple data (SIMD) instruction LCC

Semi-Global Leaderless Consensus of Linear Multi-agent Systems with Actuator and Communication Constraints

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Journal of Systems Science & Complexity 2020年第4期33卷 882-902页

作者： WANG jian shi Liangren GUAN Xinping Department of Automation and Key Laboratory of System Control and Information Processing of Ministry of EducationShanghai Jiao Tong UniversityShanghai 200240China Shanghai Key Laboratory of Navigation and Location-based Services and Academy of Information Technology and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

This paper studies the leaderless consensus problems of multi-agent systems with input saturation and intermittent communication over directed networks. Both the state feedback and the output feedback consensus algorithms are developed based on low gain feedback approach. The convergence of the trajectories of all agents can be achieved by these proposed algorithms, if the communication topology has a directed spanning tree, and the intermittent communication period T and time rate ρ are larger than their associated threshold values. Simulation examples are provided to verify the theoretical results.

关键词： agent directed communication

Sieve likelihood ratio inference on general parameter space

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Science China Mathematics 2005年第1期48卷 67-78页

作者： shi jian SHEN Xiaotong Academy of Mathematics and Systems Science Beijing 100080China School of StatisticsUniversity of MinnesotaMinneapolisMN 55455USA

In this paper,a theory on sieve likelihood ratio inference on general parameterspaces(including infinite dimensional)is *** fairly general regularity conditions,the sieve log-likelihood ratio statistic is proved to be asymptotically X^2 distributed,whichcan be viewed as a generalization of the well-known Wilks' *** an example,asemiparametric partial linear model is investigated.

关键词： likelihood ratio sieves,nonparametric and semiparametric models,wavelets.

Entropy majorization, thermal adiabatic theorem, and quantum phase transitions

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Frontiers of physics 2012年第2期7卷 244-251页

作者： shi-jian Gu (1) 1. Department of Physics and Institute of Theoretical Physics The Chinese University of Hong Kong Hong Kong China

Let a general quantum many-body system at a low temperature adiabatically cross through the vicinity of the system＇s quantum critical point. We show that the system＇s temperature is signif- icantly suppressed due to both the entropy majorization theorem in quantum information science and the entropy conservation law in reversible adiabatic processes. We take the one-dimensional transverse-field Ising model and the spinless fermion system as concrete examples to show that the inverse temperature might become divergent around the systems＇ critical points. Since the temper- ature is a measurable quantity in experiments, it can be used, via reversible adiabatic processes at low temperatures, to detect quantum phase transitions in the perspectives of quantum information science and quantum statistical mechanics.

关键词： quantum phase transition entropy majorization

Effects of CO_2 Enrichment on Photosynthesis,Growth,and Biochemical Composition of Seagrass Thalassia hemprichii (Ehrenb.) Aschers

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Journal of Integrative Plant Biology 2010年第10期52卷 904-913页

作者： Zhi jian jiang Xiao-pin Huang Jing-ping Zhang Key Laboratory of Tropical Marine Environmental Dynamics So~th China Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301 China Graduate School Chinese Academy of Sciences Beijing 100049 China

The effects of CO2 enrichment on various ecophysiological parameters of tropical seagrass Thalassia hemprichii （Ehrenb.） Aschers were tested. T. hemprichii, collected from a seagrass bed in Xincun Bay, Hainan island of Southern China, was cultured at 4 CO2（aq） concentrations in flow-through seawater aquaria bubbled with CO2. CO2 enrichment considerably enhanced the relative maximum electron transport rate （RETRmax） and minimum saturating irradiance （Ek） of T. hemprichii. Leaf growth rate of CO2- enriched plants was significantly higher than that in unenriched treatment. Nonstructural carbohydrates （NSC） of T. hemprichii, especially in belowground tissues, increased strongly with elevated CO2（aq）, suggesting a translocation of photosynthate from aboveground to belowground tissues. Carbon content in belowground tissues showed a similar response with NSC, while in aboveground tissues, carbon content was not affected by CO2 treatments. In contrast, with increasing CO2（aq）, nitrogen content in aboveground tissues markedly decreased, but nitrogen content in belowground was nearly constant. Carbon： nitrogen ratio in both tissues were obviously enhanced by increasing CO2（aq）. Thus, these results indicate that T. hemprichii may respond positively to CO2-induced acidification of the coastal ocean. Moreover, the CO2-stimulated improvement of photosynthesis and NSC content may partially offset negative effects of severe environmental disturbance such as underwater light reduction.

关键词： PHOTOSYNTHESIS PLANT growth CARBON dioxide SEAGRASSES PLANTS -- Composition BOTANICAL chemistry THALASSIA PLANT cells & tissues EFFECT of light on plants