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A protective role of resveratrol against the effects of immobilization stress in corpora lutea of mice in early pregnancy

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Journal of Integrative Agriculture 2020年第7期19卷 1857-1866页

作者： Saif ULLAH Sheeraz MUSTAFA Wael ENNAB Muhammad JAN Muhammad SHAFIQ Ngekure MXKAVITA LU Zeng-peng MAO Da-gan shi fang-xiong College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095P.R.China

In the present study,we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea(CL)of mice in early pregnancy.A total of 45 early-pregnant mice were divided into no immobilization stress(NIS)group,immobilization stress(IS)group,and immobilization and resveratrol treatment(IS+RES)group(n=15).Mice were immobilized in plastic tubes(50 mL)for 3 h per day during day 1 to 7 of *** the IS+RES group,5 mg kg-'d-1 of resveratrol was administered just prior to application of *** analyzed apoptotic activity in CL by Western botting analysis(WB),transmission electron microscopy(TEM),and immunohistochemistry(IHC).Serum progesterone levels were examined with radioimmunoassay(RIA).IHC results showed that the intensity of positive staining for Bax was increased,and for BcI-2 was decreased in CL after IS,while resveratrol treatment reversed the positive staining for Bax and *** revealed that immobilization stress up-regulated the expression of Bax and caspase-9,and down-regulated Bcl-2 expression,while resveratrol treatment attenuated the effects of immobilization stress on the expression of Bax,Bcl-2 and *** to our TEM results,apoptosis as defined by chromatin condensation was found in CL after immobilization stress,while resveratrol inhibited the *** also demonstrated that immobilization stress decreased progesterone concentrations and ovarian expression of StAR,while resveratrol restored the concentrations of progesterone and expression of StAR back to *** results indicated that immobilization stress induced luteal regression while resveratrol inhibited luteal regression,suggesting that resveratrol plays a protective role on corpora lutea of mice during early pregnancy.

关键词： immobilization stress apoptosis corpora lutea resveratrol pregnant mice

Regulatory Expression of Peroxisome Proliferator Activated Receptors Genes During Fatty Liver Formation in Geese

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Agricultural Sciences in China 2010年第1期9卷 113-120页

作者： LUO Jin-biao TIAN Yong TAO Zheng-rong YUAN Qing-yan LI Guo-qin YUAN Ai-ping ZOU Li-li LU Li-zhi SHEN Jun-da shi fang-xiong Institute of Animal Husbandry & Veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R.China

In order to investigate the expression pattern of peroxisome proliferator activated receptor （PPAR） genes before and after overfeeding, and estimate the effect of expressed PPAR levels on weights of fatty liver and abdominal fat in geese, the RT-PCR products of PPAR genes in heart, liver, spleen, lung, kidney, stomach, small intestine, brain, breast muscle, leg muscle, and abdominal fat were determined before and after overfeeding. RT-PCR was used to determine the expression levels of PPAR genes. Quantity one software was used to analyze absorbency, and the expression level of GAPDH gene was used as contrast. Expression levels of PPAR-α were relatively high in most of detected tissues but undetectable in abdominal fat tissue before overfeeding, and the level was evidently increased in lung, appeared in abdominal fat tissue, and reduced in the other tissues after overfeeding. Expressed PPAR-γ levels were relatively high in liver, spleen, lung, small intestine, and abdominal fat, and relatively low in the other tissues before overfeeding. Expressed PPAR-γ levels were enhanced in liver, spleen, lung, stomach, and kidney but decreased in abdominal fat and without obvious changes in the other tissues. Expression patterns of PPAR genes show tissue-specific manner. In addition, expression patterns of PPAR-α are different from PPAR-γ after overfeeding. It might suggest that different functions of PPAR subtypes are responsive to overfeeding.

关键词： PPAR gene RT-PCR goose fatty liver

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FOXO蛋白的修饰与细胞凋亡和癌变

生物化学与生物物理进展 2005年第7期32卷 600-606页

作者：李学斌谢庄石放雄南京农业大学动物科技学院动物繁育系南京210095

Fox蛋白家族是2000年才发布统一命名的蛋白质家族.由于其在生物体内所起的重要作用,已迅速成为生命科学研究的热点.目前,已经发现其家族成员有100多种,其中,FOXO亚家族在动物细胞的凋亡中起重要作用.细胞凋亡与动物的长寿、繁殖、代谢... 详细信息

Fox蛋白家族是2000年才发布统一命名的蛋白质家族.由于其在生物体内所起的重要作用,已迅速成为生命科学研究的热点.目前,已经发现其家族成员有100多种,其中,FOXO亚家族在动物细胞的凋亡中起重要作用.细胞凋亡与动物的长寿、繁殖、代谢、肿瘤发生及免疫有重要关系,对Forkhead(Fox)蛋白分子的命名进行了回顾、对其分类与结构特征进行了总结,并重点对FOXO的化学修饰、活性调节及其在细胞凋亡和肿瘤发生中的作用进行了综述.

关键词： FOXO蛋白化学修饰细胞凋亡癌变信号转导

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组蛋白修饰及其生物学效应

遗传 2012年第7期34卷 810-818页

作者：王维孟智启石放雄南京农业大学动物科技学院南京210095 浙江省农业科学院蚕桑研究所杭州310021

组蛋白是染色质的主要成分之一,其氨基端的氨基酸残基可以被共价修饰,进而改变染色质构型,导致转录激活或基因沉默。组蛋白修饰除了简单地调控基因表达,更在于它可以招募蛋白复合体,影响下游蛋白,从而参与细胞分裂、细胞凋亡和记忆形成... 详细信息

组蛋白是染色质的主要成分之一,其氨基端的氨基酸残基可以被共价修饰,进而改变染色质构型,导致转录激活或基因沉默。组蛋白修饰除了简单地调控基因表达,更在于它可以招募蛋白复合体,影响下游蛋白,从而参与细胞分裂、细胞凋亡和记忆形成,甚至影响免疫系统和炎症反应等。不仅如此,最近的研究表明,组蛋白修饰与CTD密码、生物节律、DNA修复之间也存在一定的联系。这些发现证明了组蛋白修饰的重要性。在组蛋白的密码形成与密码破译、修饰级联与招募蛋白质过程中,蛋白复合体的特殊结构域起到的中介作用都是无法替代的。因此,这些特殊结构域将是了解"组蛋白密码"的关键。目前质谱分析等技术的广泛应用,正使得许多新的结构域不断被发现。文章旨在对组蛋白密码的基本内容作一述评,同时对可能的研究热点进行展望。

关键词：组蛋白修饰染色质基因表达结构域蛋白复合体

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肌卫星细胞激活和补给的分子调控与肌肉疾病

生物化学与生物物理进展 2006年第9期33卷 811-815页

作者：潘玲梅王恬石放雄南京农业大学动物科技学院南京210095

肌卫星细胞(muscle satellite cell,SC)作为生肌干细胞,参与司控生后骨骼肌的生长、修复和维持等重要过程.综述了NO-HGF,Myostatin,Notch等重要信号分子及卫星细胞自身的特殊微环境对SC激活和补给的分子调控机制,希冀将来可以从这两方... 详细信息

肌卫星细胞(muscle satellite cell,SC)作为生肌干细胞,参与司控生后骨骼肌的生长、修复和维持等重要过程.综述了NO-HGF,Myostatin,Notch等重要信号分子及卫星细胞自身的特殊微环境对SC激活和补给的分子调控机制,希冀将来可以从这两方面入手克服目前临床中肌卫星细胞移植治疗各种骨骼肌疾病的瓶颈.

关键词：肌卫星细胞激活补给肌病

Anas platyrhynchos BLVRA Gene:Molecular Cloning and Correlation between Green Shell Character and mRNA Expression in the Shell Gland in the Duck

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畜牧兽医学报 2011年第B12期42卷 13-19页

作者： ZHANG Bao-le LI Guo-qin ZHANG He SHEN Jun-da LI Jin-jun TAO Zheng-rong CHEN Yi-chu WANG De-qian CHEN Wen-biao shi fang-xiong LU Li-zhi Institute of Animal Husbandry and Veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China Jinyun Husbandry and Veterinary Bureau Jinyun 321403 China Zhejiang Wulian Agriculture And Animal Co. Ltd. Jinyun 321403 China

This study has examined the structure and function of the biliverdin reductase A (BLVRA) gene and its correlation with the green eggshell character in Jinyun ducks. The previously unknown sequences of 3' and 5' ends of duck BLVRA mRNA were cloned, using RT-PCR and 5' RACE methods with four pairs of primers designed from the known coding sequence (CDS) of the duck BLVRA gene. Gene expression levels of BLVRA in the shell gland were quantified by real-time PCR, and eggshell colors were determined objectively by the reflection coefficient. The cDNA sequence of BLVRA was comprised of 1071 nucleotides, encoding a protein of 303 amino acids with an isoelectric point of 7.15 and molecular weight of 34.3 kDa. The 5' and 3' ends of the duck BLVRA gene sequence published in the GenBank were extended by 238 and 199 bp, respectively. The deduced amino acid sequence of duck BLVRA had 95.3% identity with Gallus gallus, 95.0% with Taeniopygia guttata, 70.0% with Xenopus tropicalis,61.1% with Bos taurus, 60.5% with Homo sapiens and 59.5% with Mus musculus, which indicated that the BLVRA gene was evolutionarily conserved. The relative gene expression level of BLVRA in shell glands of Jinyun ducks with white eggshells was 3 times higher than that in ducks with dark green eggshells (P<0.01). There was a significant positive correlation between reflection coefficient of eggshells and the relative expression of the BLVRA gene (r=0.719, P<0.05). Together, these findings suggest that the BLVRA gene could be a candidate gene for the molecule breeding of ducks with green eggshells.

关键词：基因设计绿头鸭 mRNA表达克隆 VRA 基因表达水平 GenBank 氨基酸序列

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基于惯量辨识的测试转台自适应内模控制

控制理论与应用 2013年第2期30卷 201-207页

作者：朱海荣李奇顾菊平方仕雄东南大学自动化学院江苏南京210096 南通大学电气工程学院江苏南通226019

为了降低系统模型参数变化对测试转台控制系统的影响,提出了基于惯量辨识技术的自适应内模控制方案.首先建立了某型号测试转台数学模型,设计了测试转台内模控制器,在此基础上采用基于扰动转矩观测器的惯量辨识算法来辨识测试转台转动惯... 详细信息

为了降低系统模型参数变化对测试转台控制系统的影响,提出了基于惯量辨识技术的自适应内模控制方案.首先建立了某型号测试转台数学模型,设计了测试转台内模控制器,在此基础上采用基于扰动转矩观测器的惯量辨识算法来辨识测试转台转动惯量的变化情况,然后通过模糊控制器根据转动惯量的变化自动调整内模控制器的参数,从而确保了控制器的控制性能.仿真和实验结果表明,该控制方案对惯量的变化有着很强的自适应性,提高了测试转台控制系统的抗干扰性能和系统的鲁棒性,取得了较好的控制效果.

关键词：测试转台内模控制惯量辨识模糊控制自适应控制

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丙烯酰胺的生殖毒性及其影响机理

畜牧与兽医 2009年第3期41卷 96-99页

作者：王皓李健石放雄南京农业大学动物繁育研究所江苏南京210095

丙烯酰胺(acrylamide,AA)具有潜在的致癌性,且在日常生活中常见,是公共卫生、食品安全研究的热点毒性物质。近几年来对丙烯酰胺神经毒性、致癌性和遗传生殖毒性的研究方兴未艾。本文着重介绍丙烯酰胺的理化特性、代谢途径、遗传生殖毒... 详细信息

丙烯酰胺(acrylamide,AA)具有潜在的致癌性,且在日常生活中常见,是公共卫生、食品安全研究的热点毒性物质。近几年来对丙烯酰胺神经毒性、致癌性和遗传生殖毒性的研究方兴未艾。本文着重介绍丙烯酰胺的理化特性、代谢途径、遗传生殖毒性方面的研究状况及有关的影响机理。

关键词：丙烯酰胺生殖毒性诱导突变致癌

猪卵巢发育的组织学观察及磷酸二酯酶3A的表达研究

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南京农业大学学报 2010年第6期33卷 101-104页

作者：方天丁威张伟周波石放雄南京农业大学动物科技学院江苏南京210095 江苏句容农林技术职业学校畜牧兽医系江苏句容212400

为研究母猪卵巢在不同发育阶段的组织学变化规律和探讨磷酸二酯酶3A(PDE3A)在卵巢发生发育和成熟过程中的生物学意义,选用二花脸母猪胎90日龄及1日龄、20日龄、120日龄和180日龄的母猪卵巢进行组织学观察,并利用RT-PCR检测PDE3A mRNA的... 详细信息

为研究母猪卵巢在不同发育阶段的组织学变化规律和探讨磷酸二酯酶3A(PDE3A)在卵巢发生发育和成熟过程中的生物学意义,选用二花脸母猪胎90日龄及1日龄、20日龄、120日龄和180日龄的母猪卵巢进行组织学观察,并利用RT-PCR检测PDE3A mRNA的表达情况。结果显示:胎90日龄主要由原始卵泡组成;1日龄、20日龄、120日龄卵巢内特征发育明显;180日龄卵巢内的形态特点与120日龄相似。卵巢PDE3A mRNA表达水平在20日龄时与其他各个阶段相比显著降低。提示:PDE3A在猪从原始卵泡募集到初级卵泡形成过程中可能具有生物学作用。

关键词：二花脸卵巢组织学磷酸二酯酶3A(PDE3A)