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微塑料联合MEHP抑制SIRT3/SOD2诱导肝细胞线粒体损伤和氧化应激

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生态毒理学报 2024年第3期19卷 363-372页

作者：王晓曼石东星韩艳阳董亚静韩浩山西医科大学公共卫生学院太原030001 山西医科大学营养与食品科学研究所太原030001 山西医科大学煤炭环境致病与防治教育部重点实验室太原030001 山西医科大学黄河流域生态公共卫生安全研究中心太原030001

微塑料(microplastics,MPs)常与多种塑料相关产品共同暴露损害人体健康。邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)是常见的塑化剂,其主要代谢物为邻苯二甲酸单(2-乙基己基)酯(mono-2-ethylhexyl phthalate,MEHP)。MPs与MEHP进入机体后均在肝脏蓄积,因此研究MPs与MEHP的联合暴露对肝脏的毒性效应及潜在机制具有重要意义。本研究采用聚苯乙烯微塑料(polystyrene microplastics,PS-MPs)和MEHP单独及二者联合处理HepG2细胞,检测细胞活力、活性氧水平、线粒体膜电位、COXⅠ、COXⅢ、SIRT3和SOD2的蛋白表达水平。结果表明,PS-MPs和MEHP单独暴露均引起活性氧生成增加、线粒体膜电位降低。PS-MPs单独暴露也导致COXⅠ与COXⅢ蛋白表达水平降低。两者联合暴露时上述有害作用更为显著,为协同效应。进一步的分子机制研究表明,PS-MPs下调了SIRT3和SOD2蛋白表达,MEHP下调了SIRT3蛋白表达,而PS-MPs和MEHP联合暴露对SIRT3和SOD2的蛋白表达的影响表现为协同效应。综上所述,PS-MPs和MEHP联合暴露导致HepG2细胞氧化应激和线粒体损伤,其分子机制与抑制SIRT3/SOD2信号通路有关。

关键词：微塑料 MEHP HepG2细胞联合暴露氧化应激线粒体损伤 SIRT3

Length of stay and inpatient charges of total knee arthroplasty in China:analysis of a national database

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Chinese Medical Journal 2023年第17期136卷 2050-2057页

作者： Huizhong Long Chao Zeng Ying shi Haibo Wang dongxing Xie Guanghua Lei Department of Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan 410008China Hunan Key Laboratory of Joint Degeneration and Injury ChangshaHunan 410008China Hunan Engineering Research Center for Osteoarthritis ChangshaHunan 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China China Standard Medical Information Research Center ShenzhenGuangdong 518054China Clinical Trial Unit First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouGuangdong 510080China

Background:There are limited data on the resource utilization of total knee arthroplasty(TKA)in China.This study aimed to examine the length of stay(LOS)and inpatient charges of TKA in China,and to investigate their determinants.Methods:We included patients undergoing primary TKA in the Hospital Quality Monitoring System in China between 2013 and 2019.LOS and inpatient charges were obtained,and their associated factors were further assessed using multivariable linear regression.Results:A total of 184,363 TKAs were included.The LOS decreased from 10.8 days in 2013 to 9.3 days in 2019.The admission-to-surgery interval decreased from 4.6 to 4.2 days.The mean inpatient charges were 61,208.3 Chinese Yuan.Inpatient charges reached a peak in 2016,after which a gradual decrease was observed.Implant and material charges accounted for a dominating percentage,but they exhibited a downward trend,whereas labor-related charges gradually increased.Single marital status,non-osteoarthritis indication,and comorbidity were associated with longer LOS and higher inpatient charges.Female sex and younger age were associated with higher inpatient charges.There were apparent varieties of LOS and inpatient charges among provincial or non-provincial hospitals,hospitals with various TKA volume,or in different geographic regions.Conclusions:The LOS following TKA in China appeared to be long,but it was shortened during the time period of 2013 to 2019.The inpatient charges dominated by implant and material charges exhibited a downward trend.However,there were apparent sociodemographic and hospital-related discrepancies of resource utilization.The observed statistics can lead to more efficient resource utilization of TKA in China.

关键词： Total knee arthroplasty China Length of stay Inpatient charges

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城市测绘单位质量体系优化与思考

地理空间信息 2024年第1期22卷 129-132页

作者：李东兴张小波刘志勇石吉宝成都市勘察测绘研究院四川成都610081 四川省城市信息化测绘工程技术研究中心四川成都610081

城市测绘地理信息工程大多体量小、总价低、数量多,且项目管理单位通常不会组织项目验收。为保障城市测绘单位产品质量的优良率,从城市测绘单位发展现状和工作内容出发,论述了质量体系优化的重要性。针对城市测绘单位质量体系面临的难题... 详细信息

城市测绘地理信息工程大多体量小、总价低、数量多,且项目管理单位通常不会组织项目验收。为保障城市测绘单位产品质量的优良率,从城市测绘单位发展现状和工作内容出发,论述了质量体系优化的重要性。针对城市测绘单位质量体系面临的难题,以城市勘测院为例,提出了工程项目组制的生产组织形式和3级检查制度的质量检审方式;并详细论述了3级检查制度的工作流程、检审内容、时间节点安排,以期能对其他城市测绘单位质量体系建设和城市测绘事业高质量发展起到积极作用。

关键词：城市测绘单位质量体系优化 3级检查制度测绘地理信息产品质量工程项目组

优化施肥对早稻中早39产量和氮肥利用率的影响

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浙江农业科学 2024年第6期65卷 1312-1315页

作者：王董兴陶娟花陈德李晓石其伟叶雪珠绍兴市柯桥区农业水产技术推广站浙江绍兴312030 浙江省农业科学院农产品质量安全与营养研究所浙江杭州310021 江苏华昌化工股份有限公司江苏苏州215634

氮肥等化肥的过量施用是我国农业和环境领域面临的主要问题之一,探索适用于区域化推广的化肥减量技术和减量比例、提高氮肥肥料利用率,意义重大。基于此,该研究以早稻品种中早39为材料,通过田间小区试验,设置不施肥(CK)、不施氮(N0)、... 详细信息

氮肥等化肥的过量施用是我国农业和环境领域面临的主要问题之一,探索适用于区域化推广的化肥减量技术和减量比例、提高氮肥肥料利用率,意义重大。基于此,该研究以早稻品种中早39为材料,通过田间小区试验,设置不施肥(CK)、不施氮(N0)、农户常规施肥(CG)和优化施肥(YH)处理,研究其对早稻产量、氮吸收、氮肥利用率等的影响。结果表明,CK和N0处理水稻产量无显著差异,CG和YH处理产量无显著差异,但显著高于CK和N0;与CK相比,CG和YH处理籽粒产量分别增加了115.96%和90.55%,秸秆产量分别增加了95.32%和67.89%,籽粒中N含量分别增加了31.0%和33.7%,CG和YH处理水稻籽粒N吸收量分别增加了1.8和1.5倍;CG和YH处理水稻氮肥利用率分别为40.90%和43.08%;与CK相比,不同施肥处理土壤pH值、有机质和全氮含量总体上无显著变化。本研究表明,N是影响水稻产量的决定因子,在当前的试验条件下施用包膜尿素并减少20%的氮肥用量能够实现水稻的稳产。

关键词：化肥减量肥料利用率包膜尿素水稻产量氮吸收量

不同有机肥与化肥配施对茶叶产量和品质的影响

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浙江农业科学 2024年第2期65卷 296-300页

作者：王董兴陶娟花石其伟王亚楠马进川绍兴市柯桥区农业水产技术推广站浙江绍兴312030 浙江省农业科学院环境资源与土壤肥料研究所浙江杭州310021 浙江农林大学环境与资源学院浙江杭州311300

以龙井43为材料,研究茶渣有机肥、生物有机肥和蚕沙有机肥分别与化肥配施对茶叶产量和品质的影响。结果表明,3种有机肥与化肥配施均可显著提高土壤pH值,增加速效磷含量、速效钾含量和有机质含量,其中蚕沙有机肥与化肥配施对土壤基础理... 详细信息

以龙井43为材料,研究茶渣有机肥、生物有机肥和蚕沙有机肥分别与化肥配施对茶叶产量和品质的影响。结果表明,3种有机肥与化肥配施均可显著提高土壤pH值,增加速效磷含量、速效钾含量和有机质含量,其中蚕沙有机肥与化肥配施对土壤基础理化指标的提升效果最为明显;与其他有机肥和化肥配施相比,蚕沙有机肥与化肥配施增产效果最为显著,增产幅度达33.9%;茶渣有机肥、生物有机肥与化肥配施处理均可显著提高叶片中总叶绿素的含量,提高幅度分别为16.8%和15.4%,其中对叶绿素b含量的提升作用优于叶绿素a。蚕沙有机肥与化肥配施可显著提高叶片中游离氨基酸含量,提高幅度达16.5%;生物有机肥与化肥配施可显著提高叶片中茶多酚含量,提高了25.4%。本研究结果可为基于产量和品质的茶叶生产过程中有机肥的施用提供参考。

关键词：有机肥茶叶品质

Persistence of VRC01-resistant HIV-1 during antiretroviral therapy

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Science China(Life Sciences) 2014年第1期57卷 88-96页

作者： GUO dongxing shi XuanLing SONG DingKa ZHANG LinQi Beijing Tropical Medicine Research Institute Beijing Friendship Hospital Capital Medical University Comprehensive AIDS Research Center and Research Center for Public Health School of Medicine Tsinghua University

VRC01,a broadly neutralizing monoclonal antibody(bnmAb),can neutralize a diverse array of HIV-1 isolates by mimicking CD4 binding to the envelope glycoprotein gp120.We have previously demonstrated the presence of VRC01-resistant strains in an HIV-1 infected patient during antiretroviral therapy.Here,we report follow-up studies of two subsequent samples from the same patient.With genetic and phenotypic analysis of over 70 full-length molecular clones of the HIV-1 envelope,we show that VRC01-resistant HIV-1 continued to exist and change in its proportion of the infecting virus during treatment with a highly active antiretroviral therapy.Consistent with our previous observation,the resistant phenotype was associated with a single asparagine residue at position 460(N460),a potential N-linked glycosylation site in the V5 region.The persistence and continuing evolution of VRC01-resistant HIV-1 in vivo presents a great challenge to our future preventative and therapeutic interventions based on VRC01.

关键词： HIV-1 逆转录病毒耐药菌株持久性治疗单克隆抗体包膜糖蛋白表型分析

Development of mechatronic driving system for seed meters equipped on conventional precision corn planter

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International Journal of Agricultural and Biological Engineering 2015年第4期8卷 1-9页

作者： Yang Li He Xiantao Cui Tao Zhang dongxing shi Song Zhang Rui Wang Mantao College of Engineering China Agricultural UniversityBeijing 100083China

Precision planters are more and more widely used for planting corn in China.Seed meters of conventional precision corn planters are usually driven by ground wheel and chain and sprocket system at present.Because of the slippage of ground wheels and vibration of chains,planting accuracy cannot be ensured,especially at higher forward speed.To improve the planting performance of precision planters,a mechatronic driving system was designed and its field working performance was evaluated in this research.A two-row pneumatic precision planter was modified to allow simultaneously using two different driving systems,i.e,with one row unit equipped with the newly designed mechatronic driving system and the other row unit equipped with conventional mechanical driving system,and used for planting at three forward speeds(9,11 and 12 km/h)on no-tillage and rotary-tillage lands.The distances between adjacent seeds in each plot were measured and the indices of uniformity in seed spacing,quality of feeding index(QFI),missing-seeding index and precision index were analyzed.With the average 4.70%increase of QFI and 3.54%decrease of missing-seeding index,the mechatronic driving system performed better than the mechanical driving system both on no-tillage and rotary-tillage lands at each forward speed.The values of QFI,missing-seeding index and precision index of the mechatronic driving system on rotary-tillage land are comparable to those on no-tillage land,by contrast,these indices of the mechanical driving system on rotary-tillage land are significantly worse than those on no-tillage land.The result indicates that the mechatronic driving system can eliminate the effect of ground wheel slippage on planting quality and maintain the uniformity of seed distribution.Although the QFI,missing-seeding index and precision index become worse with the increase of forward speed,with the worst values of QFI of 89.93%,missing-seeding index of 5.08%and precision index of 18.92%at the highest forward speed of 12 km/h,the mechatronic driving syste

关键词： precision planter seed meter mechatronic driving system control motor wheel slippage

Nitrogen-carbon layer coated nickel nanoparticles for efficient electrocatalytic reduction of carbon dioxide

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Nano Research 2019年第5期12卷 1167-1172页

作者： dongxing Tan Chaonan Cui Jinbiao shi Zhixun Luo Bingxing Zhang Xiuniang Tan Buxing Han Lirong Zheng Jing Zhang Jianling Zhang Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of ColloidInterface and Chemical ThermodynamicsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable SpeciesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing Synchrotron Radiation Facility(BSRF) Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China

The application of nickel in electrocatalytic reduction of CO2 has been largely restricted by side reaction (hydrogen evolution reaction) and catalyst poisoning.Here we report a new strategy to improve the electrocatalytic performance of nickel for CO2 reduction by employing a nitrogen-carbon layer for nickel nanoparticles.Such a nickel electrocatalyst exhibits high Faradaic efficiency 97.5% at relatively low potential of-0.61 V for the conversion of CO2 to CO.Density functional theory calculation reveals that it is thermodynamically accomplishable for the reduction product CO to be removed from the catalyst surface,thus avoiding catalyst poisoning.Also,the catalyst renders hydrogen evolution reaction to be suppressed and hence reasonably improves catalytic performance.

关键词： CO2 reduction nickel nanoparticle nitrogen-carbon layer electrocatalytic activity desity functional theory (DFT) calculation

Supercritical CO2 produces the visible-light-responsive TiO2/COF heterojunction with enhanced electron-hole separation for highperformance hydrogen evolution

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Nano Research 2020年第4期13卷 983-988页

作者： Lifei Liu Jianling Zhang Xiuniang Tan Bingxing Zhang Jinbiao shi Xiuyan Cheng dongxing Tan Buxing Han Lirong Zheng Fanyu Zhang Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of ColloidInterface and Chemical ThermodynamicsCASResearch/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical Sciences University of Chinese Academy of SciencesBeijing 100049China Physical Science Laboratory Huairou National Comprehensive Science CenterBeijing 101400China Beijing Synchrotron Radiation Facility(BSRF) Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China

To construct the heterojunctions of TiO2 with other compounds is of great importance for overcoming its inherent shortages and improving the visible-light photocatalytic performance.Here we propose the construction of TiO2/covalent organic framework(COF)heterojunction with tight connection by a supercritical CO2(SC CO2)method,which helps bridging the transformation paths for photo-induced charge between T i02 and COF.The produced T i02/COF heterojunction performs a H2 evolution of 3,962 nmol·g^-1·h^-1 under visible-light irradiation,which is-25 times higher than that of pure TiO2 and 4.5 folds higher than that of TiO2/COF synthesized by the conventional solvothermal method.This study opens up new possibilities for constructing heterojunctions for solar energy utilization.

关键词： supercritical CO2 covalent organic fram ework(COF) TiO2 H2 production visible light