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Liposome trade-off strategy in mitochondria-targeted NIR-cyanine: balancing blood circulation and cell retention for enhanced antitumor phototherapy in vivo

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Nano Research 2021年第7期14卷 2432-2440页

作者： Xianghan Zhang Sumei Zhao Zhiqing Gao Jialin Zhou Yuqiong Xia Jie Tian changhong shi Zhongliang Wang Engineering Research Center of Molecular&Neuroimaging Ministry of EducationSchool of Life Science and TechnologyXidian UniversityXi’an 710026China Key Laboratory of Molecular Imaging Institute of AutomationChinese Academy of SciencesBeijing 100190China Laboratory Animal Center Fourth Military Medical UniversityXi’an 710032China

Cancer phototheranostics involving optical imaging-guided photodynamic therapy(PDT)and photothermal therapy(PTT)is a localized noninvasive approach in treating ***-targeted near-infrared(NIR)cyanines are excellent therapeutic photosensitizers of ***,most mitochondria-targeted cyanines exist in the form of hydrophobic structures,which in vivo may cause cyanine aggregation during blood circulation,resulting in poor biocompatibility and limited therapeutic ***,we developed a trade-off strategy by encapsulating mitochondria-targeted cyanines into liposomal bilayers(CyBI7-LPs),which balanced hydrophilicity that favored blood circulation and hydrophobicity that enhanced mitochondria tumor ***,CyBI7-LPs greatly minimized photobleaching of cyanine as self-generated reactive oxygen species(ROS)could rapidly escape from the liposomal bilayer,affording enhanced PTT/PDT ***-mediated targeting strategy was further employed to improve uptake of tumor cells by modifying the liposomal surface to generate *** CyBI7-LPB probe produced a tumor-to-background ratio(TBR)of approximately 6.4 at 24 *** by highly sensitive imaging resulted in excellent anti-tumor therapy outcomes using CyBI7-LPB due to the enhanced photothermal and photodynamic *** proposed liposomal nanoplatform exhibited a simple and robust approach as an imaging-guided synergistic anti-tumor therapeutic strategy.

关键词： cancer phototheranostics NIR cyanine liposome mitochondria-targeted phototherapy

Establishment of organoid models for pancreatic ductal adenocarcinoma and screening of individualized therapy strategy

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Animal Models and Experimental Medicine 2023年第5期6卷 409-418页

作者： Miaomiao Gong Han Meng Dengxu Tan Peng Li Jing Qin Qingling An changhong shi Jiaze An Division of Cancer Biology Laboratory Animal CenterFourth Military Medical UniversityXi'anChina School of Basic Medical Sciences Medical College of Yan'an UniversityYananChina Animal Experiment Center Guangzhou University of Chinese MedicineGuangzhouChina Department of Hepatobiliary and Pancreaticosplenic Surgery Xijing HospitalFourth Military Medical UniversityXi'anChina

Background:Patients with pancreatic ductal adenocarcinoma(PDAC)who undergo surgical resection and receive effective chemotherapy have the best chance for longterm ***,because of the heterogeneity of pancreatic cancer,it is difficult to find a personalized treatment strategy for *** are ideal preclinical models for personalized ***,we explore the cultivation conditions and construction methods of PDAC organoid models to screen the individualized therapy ***:Fresh PDAC tissues from surgical resection were collected and digested with digestive enzymes;then the tumor cells were embedded in Matrigel with a suitable medium to establish the PDAC organoid *** genetic stability of the organoids was analyzed using whole exon sequencing;hematoxylin and eosin staining and immunohistochemistry of organoids were performed to analyze their consistency with the pathological morphology of the patient's tumor tissue;After 2 days of organoid culture,we selected four commonly used clinical chemotherapy drugs for single or combined treatment to analyze drug ***:Two cases of PDAC organoid models were successfully established,and the results of their pathological characteristics and exome sequencing were consistent with those of the patient's tumor *** PDAC organoids showed more sensitivity to gemcitabine and cisplatin,and the combined treatment was more effective than ***:Both organoids better retained the pathological characteristics,genomic stability,and heterogeneity with the original *** PDAC organoids exhibited different sensitivities to the same ***,this study provided ideal experimental models for screening individualized therapy strategy for patients with PDAC.

关键词： 3D culture individualized therapy organoid pancreatic ductal adenocarcinoma

Neurotrophins and neural stem cells in posttraumatic brain injury repair

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Animal Models and Experimental Medicine 2024年第1期7卷 12-23页

作者： Wenwen Guo Ke Liu Yinghua Wang Xu Ge Yifan Ma Jing Qin Caiqin Zhang Ya Zhao changhong shi Laboratory Animal Center Fourth Military Medical UniversityXi'anP.R.China Gansu University of Traditional Chinese Medicine LanzhouP.R.China Medical College of Yan'an University Yan'anP.R.China

Traumatic brain injury(TBI)is the main cause of disability,mental health disorder,and even death,with its incidence and social costs rising *** different treatment strategies have been developed and tested to mitigate neurological decline,a definitive cure for these conditions remains *** have revealed that vari-ous neurotrophins represented by the brain-derived neurotrophic factor are the key regulators of neuroinflammation,apoptosis,blood-brain barrier permeability,neurite regeneration,and memory *** factors are instrumental in alleviating neu-roinflammation and promoting *** addition,neural stem cells(NSC)contribute to nerve repair through inherent neuroprotective and immunomodulatory properties,the release of neurotrophins,the activation of endogenous NSCs,and in-tercellular ***,innovative research proposals are emerging to combine BDNF and NSCs,enabling them to synergistically complement and promote each other in facilitating injury repair and improving neuron differentiation after *** this review,we summarize the mechanism of neurotrophins in promoting neurogen-esis and restoring neural function after TBI,comprehensively explore the potential therapeutic effects of various neurotrophins in basic research on TBI,and investigate their interaction with *** endeavor aims to provide a valuable insight into the clinical treatment and transformation of neurotrophins in TBI,thereby promoting the progress of TBI therapeutics.

关键词： mutual effect neural stem cells neurological function neurotrophins traumatic brain injury

Design of multifunctional interfaces on ceramic solid electrolytes for high-performance lithium-air batteries

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Green Energy & Environment 2025年第1期10卷 183-192页

作者： Yunxin shi Ziyang Guo changhong Wang Mingze Gao Xiaoting Lin Hui Duan Yonggang Wang Xueliang Sun College of Energy Material and Chemistry College of Chemistry and Chemical EngineeringInner Mongolia UniversityHohhot010021China Department of Mechanical and Materials Engineering Western UniversityLondonONN6A 5B9Canada Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New EnergyiChEM(Collaborative Innovation Center of Chemistry for Energy Materials)Fudan UniversityShanghai200433China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315100China

High-energy-density lithium(Li)–air cells have been considered a promising energy-storage system,but the liquid electrolyte-related safety and side-reaction problems seriously hinder their *** address these above issues,solid-state Li–air batteries have been widely ***,many commonly-used solid electrolytes generally face huge interface impedance inLi–air cells and also showpoor stability towards ambient air/Li ***,we fabricate a differentiating surface-regulated ceramic-based composite electrolyte(DSCCE)by constructing disparately LiI-containing polymethyl methacrylate(PMMA)coating and Poly(vinylidene fluoride-co-hexafluoropropylene)(PVDF-HFP)layer on both sides of Li_(1.5)Al_(0.5)Ge_(1.5)(PO_(4))_(3)(LAGP).The cathode-friendly LiI/PMMA layer displays excellent stability towards superoxide intermediates and also greatly reduces the decomposition voltage of discharge products in Li–air ***,the anode-friendly PVDF-HFP coating shows low-resistance properties towards ***,Li dendrite/passivation derived from liquid electrolyte-induced side reactions and air/I-attacking can be obviously suppressed by the uniformand compact composite *** a result,the DSCCE-based Li–air batteries possess high capacity/low voltage polarization(11,836mAh g^(-1)/1.45Vunder 500mAg^(-1)),good rate performance(capacity ratio under 1000mAg^(-1)/250mAg^(-1) is 68.2%)and longterm stable cell operation(~300 cycles at 750 mA g^(-1) with 750 mAh g^(-1))in ambient air.

关键词： Li-air batteries Li_(1.5)Al_(0.5)Ge_(1.5)(PO_(4))_(3) Polymers Composite electrolyte Ambient air

Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p

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Journal of Sport and Health Science 2022年第4期11卷 495-508页

作者： Jing Lou Jie Wu Mengya Feng Xue Dang Guiling Wu Hongyan Yang Yan Wang Jia Li Yong Zhao changhong shi Jiankang Liu Lin Zhao Xing Zhang Feng Gao Center for Mitochondrial Biology and Medicine The Key Laboratory of Biomedical Information Engineering of Ministry of EducationSchool of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049China School of Aerospace Medicine Fourth Military Medical UniversityXi’an 710032China Medical School of Chinese PLA Beijing 100853China Laboratory Animal Center Fourth Military Medical UniversityXi’an 710032China

Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial *** previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as ***,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains ***:A 9-day treadmill training was used as an exercise model in C57BL/6 ***-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in ***:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in *** increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in *** of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in ***,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in ***:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair.

关键词： Angiogenesis Endothelial cells Exerkine Extracellular vesicle Metabolic shift

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前列腺癌异质性转化PDX模型研究进展

中国实验动物学报 2021年第2期29卷 242-247页

作者：师长宏空军军医大学实验动物中心西安710032

异质性是前列腺癌的主要临床特征,也是影响其治疗效果的主要因素。建立基于临床肿瘤标本的异种移植转化模型,对于前列腺癌的研究具有重要意义。本文就前列腺癌的临床异质性进行总结,综述模拟临床特征的PDX模型研究进展,重点关注急需的... 详细信息

异质性是前列腺癌的主要临床特征,也是影响其治疗效果的主要因素。建立基于临床肿瘤标本的异种移植转化模型,对于前列腺癌的研究具有重要意义。本文就前列腺癌的临床异质性进行总结,综述模拟临床特征的PDX模型研究进展,重点关注急需的异质性转化模型,以期为前列腺癌的临床转化机制研究和靶向药物筛选提供理想的动物模型。

关键词：异质性转化前列腺癌病人来源异种移植模型

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miRNA调控诱导的随机增益和双峰表达

江西师范大学学报（自然科学版） 2016年第1期40卷 15-21页

作者：施昌宏广州医科大学公共卫生学院广东广州510275

一方面,miRNA通过加速mRNA的降解对基因表达进行后转录调控;另一方面,最新的研究表明某些细胞中miRNA以切换的方式对mRNA进行调控.基于此,建立起一个miRNA后转录调控的基因表达模型.通过对相应化学主方程的分析求解与数值模拟,研究了mi... 详细信息

一方面,miRNA通过加速mRNA的降解对基因表达进行后转录调控;另一方面,最新的研究表明某些细胞中miRNA以切换的方式对mRNA进行调控.基于此,建立起一个miRNA后转录调控的基因表达模型.通过对相应化学主方程的分析求解与数值模拟,研究了miRNA调控过程中的噪声对mRNA表达的定量和定性影响.结果显示:这种随机涨落既能提高mRNA的平均表达水平(称这种现象为随机增益)又能诱导m RNA的双峰表达.研究结果表明:miRNA后转录调控是一种有效控制基因表达的机制.

关键词： miRNA 后转录调控随机增益双峰表达基因模型

Immune Responses and Protective Efficacy Induced by 85B Antigen and Early Secreted Antigenic Target-6 kDa Antigen Fusion Protein Secreted by Recombinant Bacille Calmette-Guérin

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 290-296页

作者： changhong shi~1* Xiaowu WANG~1 Hai ZHANG~1 Zhikai XU~2 Yuan LI~2 Lintian YUAN~11 Laboratory Animal Researeh Center,Fourth Military Medical University,Xi’an 710033,China2 Department of Microbiology,Fourth Military Medical University,Xi’an 710033,China Laboratory Animal Research Center Fourth Military Medical UniversityXi'an 710033 China Department of Microbiology Fourth Military Medical UniversityXi'an 710033 China

In an attempt to improve immune responses and protective efficacy,we constructed tworecombinant bacille Calmette-Guérin(rBCG)strains expressing an 85B antigen(Ag85B)and early secretedantigenic target-6 kDa antigen(ESAT6)of M vcobacterium tuberculosis(MTB)fusion *** rBCGstrains have the same protein insertion but in a different order(Ag85B-ESAT6 and ESAT6-Ag85B).Thecultured supernatant of rBCG strains and the sera from the mice immunized with the fusion protein Ag85B-ESAT6 or ESAT6-Ag85B formed a band with a fraction size of 37 kDa,equalivalent to the sum of Ag85B *** weeks after BALB/c mice were immunized with BCG or rBCG,spleen lymphocytes showedsignificant proliferation in response to culture filtrate protein of *** with the BCG group,micevaccinated with rBCG elicited a high level increase of immunoglobulin G antibodies to culture filtrate proteinin the *** γ-interferon levels in the lymphocyte culture medium supernatants increased remarkably inthe rBCG1 group,significantly higher than that of the BCG immunized group(P0.05).

关键词： Mycobacterium tuberculosis vaccine Ag85B ESAT6 BCG

基于近红外荧光制剂的多模态多功能分子影像技术在肿瘤模型中的应用

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中国实验动物学报 2018年第2期26卷 234-238页

作者：师长宏第四军医大学实验动物中心西安710032

多模态融合的分子影像技术整合了多种分子影像技术的优势,已成为当前分子影像领域研究的热点和发展趋势。新近报道的七甲川菁(heptamethine cyanine)染料是一类具有肿瘤靶向性的近红外荧光(nearinfrared fluorescence,NIRF)制剂。该染... 详细信息

多模态融合的分子影像技术整合了多种分子影像技术的优势,已成为当前分子影像领域研究的热点和发展趋势。新近报道的七甲川菁(heptamethine cyanine)染料是一类具有肿瘤靶向性的近红外荧光(nearinfrared fluorescence,NIRF)制剂。该染料独特的光学特征使其在肿瘤分子影像、靶向治疗和药物传递系统方面具有广阔的应用前景。纳米材料包裹该类染料可用于NIRF/MRI双模成像,标记核素后可实现NIRF/PET双模成像,共轭结合化疗药物后,可实现抗肿瘤药物的靶向传递,多个七甲川菁染料的复合物已被用作多模态成像,成为光热、光动力学和组合治疗肿瘤的新策略。

关键词：近红外荧光多模态成像肿瘤模型分子影像