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Germline mutation analysis of hPMS2 gene in Chinese families with hereditary nonpolyposis colorectal cancer

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World Journal of Gastroenterology 2010年第30期16卷 3847-3852页

作者： Xia sheng, xiao-yan Zhou, Xiang Du, Tai-Ming Zhang, WeiQi sheng, Da-Ren Shi, Department of Pathology, Shanghai Cancer Center, Fudan University, Shanghai 200032, China Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China Heng-Hua Zhou, Department of Pathology, Shanghai Ninth People’s Hospital Affi liated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China San-Jun Cai, Department of Abdominal Surgery, Cancer Center, Fudan University, Shanghai 200032, China Department of Pathology Shanghai Cancer Center Fudan University Shanghai 200032 China.

AIM: To study the germline mutation of hPMS2 gene in 26 unrelated Chinese hereditary nonpolyposis colorectal cancer (HNPCC) probands and to fulfill the screening strategy for HNPCC in Chinese. METHODS: Genomic DNA was extracted from the peripheral blood. To avoid the interference of pseudogene in detection of the remaining 11 exons (exon 1-5, 9, 11-15), long-range polymerase chain reaction (PCR) was conducted to amplify the complete coding region of hPMS2 gene firstly. Then 1/8 of the PCR productswere used as template to amplify the individual exon respectively and DNA sequencing was done. Direct DNA sequencing of the conventional PCR products of exon 6, 7, 8 and 10 of hPMS2 gene was performed. The same analysis was made in 130 healthy persons without family histories of HNPCC to further investigate the pathological effects of the detected missense mutation. RESULTS: One HNPCC proband fulf illed Bethesda guidelines and was found to carry the germline mutation of hPMS2 gene, which has not been reported in Chinese HNPCC families. It was a missense mutation at c.1532C>T of exon 11. It was detected in three controls as well with an occurrence rate of 2.3% (3/130). Since it could not be found in the PMS2-single nucleotide polymorphism (SNP) database, this missense mutation is a new SNP unreported up to date. Meanwhile, 260 reported SNPs of hPMS2 gene were detected in the 26 HNPCC probands. The 2nd and 5th exons were probably the hot SNP regions of hPMS2 gene in Chinese HNPCC families involving 53.1% of all reported SNP. CONCLUSION: The germline mutation of hPMS2 gene may be rare in Chinese HNPCC families. The 2nd and 5th exons are hot SNP regions of hPMS2 gene.

关键词： Hereditary nonpolyposis colorectal cancer hPMS2 Missense mutation Single nucleotide polymorphism Colorectal cancer

Pharmacokinetics of sufentanil administered by target-controlled infusion in Chinese surgical patients

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Chinese Medical Journal 2009年第3期122卷 291-295页

作者： ZHAO yan WU Xin-min DUAN Jing-li sheng xiao-yan LIU Wei LU Wei ZHANG Li-ping XU Chuan-ya Department of Anesthesiology Peking University Third Hospital Beijing 100191 China Department of Pharmacy Peking University Third Hospital Beijing 100191 China Department of Anesthesiology Peking University First HospitalBeijing 100034 China School of Pharmaceutical Science Peking University Beijing100191 China

Background Target-controlled infusion （TCI） has been recently developed and successfully implemented in clinical practice. This study was conducted to determine the pharmacokinetics of TCI administered sufentanil in Chinese surgical patients. Methods The pharmacokinetics of sufentanil was investigated in 12 adult patients, aged 23-76 years, scheduled for prolonged surgery under general anesthesia. Anesthetic induction was carried out with propofol, rocuronium and TCI administered sufentanil aiming for target effect-site concentration of sufentanil 4 or 6 ng/ml. Sufentanil TCI lasted for 30 minutes. Frequent arterial blood samples （1.5 ml） were drawn during and up to 24 hours after sufentanil TCI. Plasma sufentanil concentrations were measured by liquid chromatography-tandem mass spectrometry; limit of sensitivity of mass spectrometry was 5 pg/ml. The data were analyzed with the nonlinear mixed-effect model program. Results The pharmacokinetics of TCI administered sufentanil were optimally described by a three-compartment model with the following parameters： the central volume of distribution （V1） = 5.4 L, the volume of distribution at steady-state （Vdss） = 195.4 L, systemic clearance （CI1） = 1.10 L/min, and elimination half-life （t1/2 Y） = 271.8 minutes. Both age and gender affected the pharmacokinetic parameters. The rapid distribution clearance （012） was negatively correlated with patient age, and the volume of slowly equilibrating compartment （V3） was positively correlated with age. The Cl2 and the volume of rapidly equilibrating compartment （V2） were influenced by gender with male patients showing higher values of Cl2 and V2 than female patients. There was no relationship of body weight, lean body mass, plasma albumin, or target effect-site concentration of sufentanil with any of the pharmacokinetic parameters studied. Conclusions The pharmacokinetics of TCI administered sufentanil in Chinese patients can be adequately described by a three-compartment model. Pha

关键词： sufentanil pharmacokinetics target-controlled infusion tandem mass spectrometry mixed-effect model

Development Model and Characteristics of Rural Land Joint Stock-cooperative System

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Asian Agricultural Research 2012年第3期4卷 51-53页

作者： CHANG Huan-huan,YU Yun,GUO Yong-sheng,CHEN xiao-di yan’an University,yan’an 716000,China

We introduce and analyze operating mechanism of three major rural land joint-stock cooperative systems in China,including community type(Nanhai model),collective leasing type(Ningxia Pingluo model) and joint operating type(Zhejiang Jiande model).Current rural land joint-stock cooperative system has following ***,adhere to the collective ownership and household contract responsibility system in rural ***,safeguard farmers' land contractual rights and ***,implement large-scale agricultural production and ***,carry out normative operation of farmers' *** the basis of these,we point out such problems as backward technology and short of funds in development of rural land joint-stock cooperative ***,we conclude that the development of rural land joint-stock cooperative system should fully draw lessons from foreign advanced experience,actively try new model like securitization of rural land,to explore a road suitable for current national conditions.

关键词： Rural land joint-stock cooperative system Househol

Clinical evaluation of target controlled infusion system for sufentanil administration

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Chinese Medical Journal 2009年第20期122卷 2503-2508页

作者： ZHAO yan ZHANG Li-ping WU Xin-min JIANG Jian-yu DUAN Jing-li HU Yong-fang LI Min LIU Wei sheng xiao-yan NI Cheng XU Mao GUO Xiang-yang Department of Anesthesiology Peking University Third Hospital Beijing 100191 China Department of Anesthesiology Peking University First Hospital Beijing 100034 China Department of Pharmacy Peking University Third Hospital Beijing 100191 China

Background Sufentanil target controlled infusion （TCI） provides stable analgesia, better hemodynamic control than a bolus injection of intravenous anesthetics, anticipated recovery and improved quality of anesthesia during perioperative period. This study evaluated the accuracy and feasibility of TCI system for sufentanil at high concentrations in Chinese surgical patients. Methods Twelve low risk adult patients undergoing elective surgery under general anesthesia were included in this study. Sufentanil was administered with a specific TCI system incorporating the population pharmacokinetic data of sufentanil previously reported, using a target effect-site concentration of sufentanil 4 or 6 ng/ml. Sufentanil TCI duration was 30 minutes. Frequent arterial blood samples were taken during and up to 24 hours after sufentanil TCI for determination of plasma sufentanil concentrations by liquid chromatography-mass spectrometry/mass spectrometry. The changes of circulatory system function during the procedure, recovery profile and adverse effects were recorded. Measured plasma sufentanil concentrations were compared with the values predicted by the TCI system. The bias （median performance error, MDPE）, precision （median absolute performance error, MDAPE） and wobble （variability of performance error） of the sufentanil TCI system were determined. Results All patients had stable cardiovascular variables during induction and maintenance of anesthesia. Time to eye opening and extubation were （5.6±1.7） minutes when TCI set to 4 ng/ml and （7.2±2.3） minutes when set to 6 ng/ml. There was no episode of agitation, muscle rigidity or intraoperative awareness. The bias （MDPE）, precision （MDAPE） and wobble of the sufentanil TCI system were -3.7%, 18.9% and 19.6% respectively during TCI, and the MDPE, MDAPE and wobble were -29.1%, 31.7% and 15.0% respectively after TCI （up to 8 hours）. Conclusions The TCI system programmed for sufentanil at 4 or 6 ng/ml was considered acceptable for clin

关键词： anesthesia analgesic sufentanil target-controlled infusion tandem mass spectrometry

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia yang Qian-Ru He Fei Ding Hai-yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Two-stage analysis of pharmacokinetics of sufentanil administered by target-controlled infusion in Chinese patients

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Chinese Medical Journal 2009年第17期122卷 1979-1984页

作者： ZHAO yan DUAN Jing-li WU Xin-min JIANG Jian-yu LU Wei ZHANG Li-ping WANG Jun MENG Xiu-li XU Chuan-ya JIA Dong-lin LIU Wei sheng xiao-yan Department of Anesthesiology (Zhao Y Jiang JY Zhang LP WangJ Meng XL Xu CY and Jia DL) Department of Pharmacy Peking University Third HospitalBeijing 100191 China Department of Anesthesiology Peking University First HospitalBeijing 100034 China School of Pharmaceutical Sciences Peking University Beijing100191 China

Background Sufentanil is a suitable choice for target-controlled infusion （TCI） because of its shorter context-sensitive half-time. The current study was to estimate the pharmacokinetics of sufentanil TCI in Chinese patients using the two-stage analysis. Methods Twelve adult patients with American Society of Anesthesiologists （ASA） physical status I or II undergoing elective surgery under general anesthesia were included. Anesthesia was induced with propofol, rocuronium and sufentanil administered by TCI lasting for 30 minutes, with target effect-site concentration of sufentanil 4 or 6 ng/ml. Frequent arterial blood samples （1.5 ml） were taken during and up to 24 hours after sufentanil TCI. Before the end of surgery, another arterial blood sample （1.0 ml） was drawn for the blood-gas analysis. Plasma sufentanil concentrations were determined by liquid chromatography-tandem mass spectrometry （limit of quantitation was 5 pg/ml）. The data were analyzed with the two-stage approach, linear regression and correlation analysis. Results The pharmacokinetics of sufentanil TCI were adequately described by a three-compartment model. The variables were derived as follows： the volume of central compartment （V1） was 5.4 L, volume of distribution at steady-state （Vdss） was 222.6 L, metabolic clearance （CI1） was 0.84 L/min and elimination half-life （t~/2y） was 389 minutes. Patients＇ age, gender and PaCO2 correlated significantly with the pharmacokinetic parameters. The Vdss, volume of slowly equilibrating compartment （V3） and t1/2 y increased, and rapid distribution clearance （012） decreased with increasing patient age. Male patients had larger values of Vdss, volume of rapidly equilibrating compartment （V2） and V3 than female patients. The Vdss and V3 increased with higher PaCO2 values. There were no significant correlations between the pharmacokinetic variables and body weight, height, lean body mass, plasma albumin, sufentanil dose, duration of surgery, pH or base excess of blood

关键词： anesthesia sufentanil pharmacokinetics target-controlled infusion two-stage analysis

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数字式气体活塞压力计的活塞系统设计

测控技术 2012年第7期31卷 13-15页

作者：王丽盛晓岩北京长城计量测试技术研究所北京100095

介绍了数字式气体活塞压力计的关键部件——活塞系统的设计方法,详细阐述了活塞系统的设计原则、材料的选择以及关键尺寸的设计,并通过了活塞系统性能指标和数字式活塞压力计性能指标的验证,为数字式活塞压力计技术的迅速发展提供了理... 详细信息

介绍了数字式气体活塞压力计的关键部件——活塞系统的设计方法,详细阐述了活塞系统的设计原则、材料的选择以及关键尺寸的设计,并通过了活塞系统性能指标和数字式活塞压力计性能指标的验证,为数字式活塞压力计技术的迅速发展提供了理论依据。

关键词：数字式气体活塞压力计活塞系统设计原则尺寸设计验证

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang yanlin Fu Peiyan Long xiao Gao Zhengwei Wang Zhizhong Guan xiaolan Qi Wei Hong yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

急性症状性PVD致视网膜裂孔合并玻璃体积血

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国际眼科杂志 2010年第7期10卷 1368-1369页

作者：李慧丽肖胜燕中国重庆市第八人民医院眼科 400015

目的:观察急性症状性玻璃体后脱离致视网膜裂孔合并玻璃体积血的临床特点和治疗效果。方法:回顾分析8例因为急性症状性玻璃体后脱离致视网膜裂孔合并玻璃体积血患者的临床资料。结果:经双眼遮盖、半卧位及应用止血剂等对症处理,眼底可... 详细信息

目的:观察急性症状性玻璃体后脱离致视网膜裂孔合并玻璃体积血的临床特点和治疗效果。方法:回顾分析8例因为急性症状性玻璃体后脱离致视网膜裂孔合并玻璃体积血患者的临床资料。结果:经双眼遮盖、半卧位及应用止血剂等对症处理,眼底可视度增加,及时予激光光凝治疗,视网膜裂孔封闭,无视网膜脱离发生,疗效满意。结论:充分认识急性症状性玻璃体后脱离致视网膜裂孔合并玻璃体积血的临床特点,认真检查、寻找裂孔,及时行激光光凝封孔治疗是争取较好预后的关键。

关键词：急性症状性玻璃体后脱离视网膜裂孔玻璃体积血激光光凝