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Xiao Tan San Jie Fang Hampers the Growth of Colon Cancer Stem Cells through the Wnt/β-catenin Signaling Pathway

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World Journal of Traditional Chinese Medicine 2024年第2期10卷 171-179页

作者： Yu-Qi Zhou Jun-Fei Yuan Hong-shi shen Ya-Ping Wang Han-Fei shi De-Jian Pan Min Ye Department of Hematology and Oncology The 904th Hospital of PLA Joint Logistic Support Force Department of Clinical Laboratory The 904th Hospital of PLA Joint Logistic Support Force Department of Traditional Chinese Medicine Second Affiliated Hospital of Naval Medical University

Objective: The purpose of this research was to examine the potential anticancer properties of Xiao Tan San Jie Fang(XTSJF) and its potential mechanism of action against colorectal cancer. Materials and Methods: HCT116 cells were induced into HCT116 spheres in DMEM/F12 medium by treatment with epidermal growth factor + fibroblast growth factor + leukemia inhibitory factor + B27. The proliferation ability and stemness of HCT116 spheres was examined. Various concentrations of XTSJF were used to treat HCT116 spheres to observe the impact on proliferation, apoptosis, and expression of stem cell markers. Next, Wnt/β-catenin pathway-related factor proteins were detected. Results: The findings revealed that XTSJF suppressed cell growth and induced cell death in HCT116 cells in a dosage-dependent manner. Similarly, XTSJF promotes apoptosis, inhibits cell proliferation, prolongs survival, and maintains the expression of stem cells through the Wnt/-catenin/TCF4 axis. XTSJF also inhibits AKT activity and subsequently activates glycogen synthesis kinase-3β expression, inhibiting Wnt/beta-catenin pathway activity and downstream target gene transcript expression. The Wnt/β-catenin signaling pathway is inhibited by the XTSJF, leading to the suppression of colon cancer stem cell proliferation. Conclusion: Xiaotan Sanjie Prescription inhibited colon cancer stem cell growth through Wnt/β-catenin signaling pathway.

关键词： Apoptosis colorectal cancer proliferation traditional Chinese medicine Wnt/β-catenin signaling

Aggregation-induced delayed electrochemiluminescence of organic dots in aqueous media

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Aggregate

Aggregate 2024年第1期5卷 167-174页

作者： Hang Gao shen-Yu shi Shu-MinWang Qian-Qian Tao Hui-Li Ma Jun Hu Hong-Yuan Chen Jing-Juan Xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjingChina Key Laboratory of Flexible Electronics(KLOFE)and Institute of Advanced Materials(IAM) Nanjing Tech UniversityNanjingChina School of Life Science and Health Engineering Jiangnan UniversityWuxiChina

Full utilization of the excited species at both singlet states(1R*)and triplet states(3R*)is crucial to improving electrochemiluminescence(ECL)efficiency but is challenging for organic luminescent materials.Here,an aggregation-induced delayed ECL(AIDECL)active organic dot(OD)containing a benzophenone acceptor and dimethylacridine donor is reported,which shows high ECL efficiency via reverse intersystem crossing(RISC)of non-emissive 3R*to emissive 1R*,overcoming the spin-forbidden radiative decay from 3R*.By introducing dual donor-acceptor pairs into luminophores,it is found that nonradiative pathway could be further suppressed via enhanced intermolecular weak interactions,and multiple spin-up conversion channels could be activated.As a consequence,the obtained OD enjoys a 6.8-fold higher ECL efficiency relative to the control AIDECL-active OD.Single-crystal studies and theoretical calculations reveal that the enhanced AIDECL behaviors come from the acceleration of both radiative transition and RISC.This work represents a major step towards purely organic,high-efficiency ECL dyes and a direction for the design of next-generation ECL dyes at the molecular level.

关键词： aggregation-induced delayed electrochemiluminescence benzophenone dimethylacridine organic dots reverse intersystem crossing

Triclosan-induced Oxidative Stress Injury and Apoptosis by Regulating the PI3K/Akt/Caspase-3 Signaling Pathway in Human Renal Glomerular Endothelial Cells

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Biomedical and Environmental Sciences 2022年第6期35卷 547-551页

作者： MA Yan CHEN Chen WANG Jing Bo CHENG Jia Li shen shi CHEN Xi HUO Jun sheng Key Laboratory of Trace Element Nutrition National Institute for Nutrition and HealthChinese Center for Disease Control and PreventionBeijing 100050China

Triclosan(TCS)is widely used in personal-care products because of its bactericidal and antibacterial properties.However,TCS and its toxic byproducts have been detected in aquatic environments,animals,and plants worldwide.TCS is a common phenolic environmental endocrine disruptor,and TCS in the environment enters the body mainly through diet and water.Most of the absorbed TCS is metabolized by the human kidneys and is eliminated in the urine[1].Some epidemiological studies have suggested a positive correlation between the TCS exposure level in urine and albumin(a biomarker of renal function),which could cause renal dysfunction[2].However,the potential effects of TCS on the mammalian kidney and its mechanisms are currently unknown.Consequently,we initiated an in vitro trial to study the molecular mechanism of cytotoxicity of TCS to glomeruli.

关键词： PI3K/Akt urine Apoptosis

Optimization of Three-Dimensional Culture Conditions of HepG2 Cells with Response Surface Methodology Based on the VitroGel System

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Biomedical and Environmental Sciences 2022年第8期35卷 688-698页

作者： WANG Jing Bo QIN Wen YANG Zhuo shen shi MA Yan WANG Li Yuan ZHUO Qin GONG Zhao Long HUO Jun sheng CHEN Chen National Institute for Nutrition and Health Chinese Centre for Disease Control and PreventionBeijing 100050China

Objective This study optimizes three-dimensional(3D) culture conditions of HepG2 using response surface methodology(RSM) based on the VitroGel system to facilitate the cell model in vitro for liver tissues.Method HepG2 cell was 3D cultured on the VitroGel system.Cell viability was detected using Cell Counting Kit-8(CCK-8) assay of HepG2 lived cell numbers.The proliferation of HepG2 cell and clustering performance was measured via fluorescence staining test.Albumin concentration in the culture medium supernatant as an index of HepG2 cell biological function was measured with ELISA kit.Independent factor tests were conducted with three key factors:inoculated cell concentration,cultured time,and dilution degree of the hydrogel.The preliminary results of independent factor tests were used to determine the levels of factors for RSM.Result The selected optimal culture conditions are as follows:concentration of inoculated cells was4.44 × 10^(5)/mL,culture time was 4.86 days,and hydrogel dilution degree was 1:2.23.The result shows that under optimal conditions,the predicted optical density(OD) value of cell viability was 3.10 and measured 2.978 with a relative error of 3.94%.Conclusion This study serves as a reference for the 3D HepG2 culture and constructs liver tissues in vitro.Additionally,it provides the foundation for repeated dose high-throughput toxicity studies and other scientific research work.

关键词： Response surface methodology The three-dimensional culture Hydrogel

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煤矿掘进工作面瓦斯浓度预测

工矿自动化 2024年第4期50卷 128-132页

作者：陈鲜展沈易成洪飞扬石绅安徽建筑大学土木工程学院安徽合肥230011

针对目前瓦斯浓度预测方法存在数据处理不确定性、特征提取局限性及受主观性因素影响产生预测偏差等问题,提出了一种用于煤矿掘进工作面的瓦斯浓度预测方法。首先,在煤矿掘进工作面回风巷内每隔1 m布设激光瓦斯传感器,形成传感器网络,... 详细信息

针对目前瓦斯浓度预测方法存在数据处理不确定性、特征提取局限性及受主观性因素影响产生预测偏差等问题,提出了一种用于煤矿掘进工作面的瓦斯浓度预测方法。首先,在煤矿掘进工作面回风巷内每隔1 m布设激光瓦斯传感器,形成传感器网络,实时采集瓦斯浓度数据。然后,根据拉依达准则搜索并剔除瓦斯浓度数据中的异常值,并利用Lagrange插值多项式填补瓦斯浓度数据中的缺失值。最后,以剔除异常值及填补缺失值的瓦斯浓度数据为基础,采用经验模态分解算法将瓦斯浓度数据分解成本征模态函数和趋势项,再利用Hilbert变换对本征模态函数进行处理以获取数据的高频项和低频项,并将其输入最小二乘支持向量机中进行加权处理,输出瓦斯浓度预测结果。通过掘进工作面模拟装置进行瓦斯浓度预测模拟试验,并在某煤矿掘进工作面进行现场试验,结果表明:该方法预测的瓦斯浓度与实际测量值非常接近,均方误差小,表明预测结果准确率高;均方误差波动幅度小,表明适应性好,预测结果的稳定性强;预测用时短,表明预测效率高。

关键词：掘进工作面瓦斯浓度预测拉依达准则 Lagrange插值经验模态分解最小二乘支持向量机

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Cross-national Perspectives on Using Sustainable Development Goals(SDGs) Indicators for Monitoring Sustainable Development: A Database and Analysis

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Chinese Geographical Science 2021年第4期31卷 600-610页

作者： WANG Xiangyu SONG Changqing CHENG Changxiu YE Sijing shen shi Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing 100875China Center for Geodata and Analysis Faculty of Geographical ScienceBeijing Normal UniversityBeijing 100875China National Tibetan Plateau Data Center Beijing 100101China

Sustainable development is the theme of the 21st century. To monitor the progress of sustainable development, the United Nations launched Sustainable Development Goals(SDGs) in 2015. Subsequently, nations of the world have drawn up a list of localized indicators regarding the United Nations SDGs as a paradigm. We established a database including SDGs indicator systems of 11 economies by collecting and determining a large number of materials. Based on this database, we analyzed SDGs indicators by designing a conceptual framework of comparative analysis that included three views. We found that the SDGs indicator systems of 11 economies are different between the number of indicators, the proportion of different categories, and the connotation of indicators. Although the SDGs indicator systems among economies regarded the United Nations SDGs as a framework and included the major social problems related to sustainability, the inconsistency between SDGs indicator systems is large. It is a major reason why scholars lack the systematic method for developing indicators. There are challenges faced in data accessibility. The framework for comparative analysis could be applied to different economies.

关键词： Sustainable Development Goals(SDGs) framework indicator cross-national database

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建筑外立面真石漆施工技术研究

价值工程 2024年第6期43卷 118-120页

作者：施沈杰南通市建设工程质量监督站南通226000

真石漆施工技术可以有效提升建筑外立面装饰施工质量,需要对真石漆施工技术相关内容做深入研究。本文将建筑外立面真石漆施工技术作为研究对象,整理具体工程案例,从条件、技术、人员、机具等方面,叙述施工准备内容,从基层处理、刮涂腻... 详细信息

真石漆施工技术可以有效提升建筑外立面装饰施工质量,需要对真石漆施工技术相关内容做深入研究。本文将建筑外立面真石漆施工技术作为研究对象,整理具体工程案例,从条件、技术、人员、机具等方面,叙述施工准备内容,从基层处理、刮涂腻子、涂刷底漆、弹线贴纸、喷涂真石漆等方面,叙述施工流程,从使用年限、墙体质量、施工成本、施工工期等方面,整理建筑外立面真石漆施工技术应用效果,旨在为更多建筑外立面装饰施工单位提供技术思考。

关键词：建筑外立面真石漆施工技术

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论如何提高中小型水库运行管理水平

科学与信息化 2024年第6期 193-195页

作者：石申权黔南州水旱灾害防御服务中心贵州黔南 558000

文章通过分析黔南州中小型水库现状,得出了当前全州中小型水库存在运行质量及运行效率不佳、运行管理体制不健全、运行管理经费不足这三大主要问题,随后讨论了中小型水库运行管理工作要点,并针对如何提高中小型水库运行管理水平这一问... 详细信息

文章通过分析黔南州中小型水库现状,得出了当前全州中小型水库存在运行质量及运行效率不佳、运行管理体制不健全、运行管理经费不足这三大主要问题,随后讨论了中小型水库运行管理工作要点,并针对如何提高中小型水库运行管理水平这一问题提出了几点建议,希望可以为全州中小型水库运行管理发展提供参考与借鉴.

关键词：中小型水库运行管理水平提升策略

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病险水库除险加固存在的问题及对策