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Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

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mLife 2024年第1期3卷 119-128页

作者： Pengpeng Zhao Xiaonan Bi Xiaoning Wang Xu Feng yulong shen Guanhua Yuan Qunxin She CRISPR and Archaea Biology Research Center State Key Laboratory of Microbial Technology and Microbial Technology InstituteShandong UniversityQingdaoChina

Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools,which include efficient genome editing,gene silencing,and robust protein expression ***,plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic *** this plasmid coexists with pRN1 in its original host,early attempts to test pRN1-based vectors consistently failed to yield any stable host-vector system for *** hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this *** identified a putative target sequence in orf904 encoding a putative replicase on pRN1(target N1).Mutated targets(N1a,N1b,and N1c)were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid inter-ference *** results revealed that the original target triggered CRISPR immunity in this archaeon,whereas all three mutated targets did not,indicating that all the designed target mutations evaded host *** mutated targets were then incorporated into orf904 individually,yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed(pN1aSD,pN1bSD,and pN1cSD).*** transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors,but pN1cSD lost the capability for *** results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the *** further showed that pRN1-based and pRN2-based vectors were stably maintained in the archaeal cells either alone or in combination,and this yielded a dual plasmid system for genetic study with this important archaeal model.

关键词： archaeal genetics CRISPR-Cas CRISPR escape mutations dual plasmid vectors Saccharolobus-E.coli shuttle vectors

A survey on membership inference attacks and defenses in machine learning

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Journal of Information and Intelligence 2024年第5期2卷 404-454页

作者： Jun Niu Peng Liu Xiaoyan Zhu Kuo shen Yuecong Wang Haotian Chi yulong shen Xiaohong Jiang Jianfeng Ma Yuqing Zhang School of Computer Science and Technology Xidian UniversityXi'an 710071China Information Sciences and Technology Pennsylvania State UniversityPA 16802United States School of Telecommunications Engineering Xidian UniversityXian 710071China School of Hangzhou Institute of Technology Xidian UniversityHangzhou 311231China School of Automation and Software Engineering Shanxi UniversityTaiyuan 030006China School of Systems Information Science Future University of HakodateHakodate 041-8655Japan School of Computer&Control Engineering University of Chinese Academy of SciencesBeijing 101408China School of Cyber Engineering Xidian UniversityXian 710071China

Membership inference(MI)attacks mainly aim to infer whether a data record was used to train a target model or *** to the serious privacy risks,MI attacks have been attracting a tremendous amount of attention in the research *** existing work conducted-to our best knowledge the first dedicated survey study in this specific area:The survey provides a comprehensive review of the literature during the period of 2017~2021(e.g.,over 100 papers).However,due to the tremendous amount of progress(i.e.,176 papers)made in this area since 2021,the survey conducted by the one existing work has unfortunately already become very limited in the following two aspects:(1)Although the entire literature from 2017~2021 covers 18 ways to categorize(all the proposed)MI attacks,the literature during the period of 2017~2021,which was reviewed in the one existing work,only covered 5 ways to categorize MI *** 13 ways missing,the survey conducted by the one existing work only covers 27%of the landscape(in terms of how to categorize MI attacks)if a retrospective view is taken.(2)Since the literature during the period of 2017~2021 only covers 27%of the landscape(in terms of how to categorize),the number of new insights(i.e.,why an MI attack could succeed)behind all the proposed MI attacks has been significantly increasing since year *** a result,although none of the previous work has made the insights as a main focus of their studies,we found that the various insights leveraged in the literature can be broken down into 10 *** making the insights as a main focus,a survey study could fail to help researchers gain adequate intellectual depth in this area of *** this work,we conduct a systematic study to address these *** particular,in order to address the first limitation,we make the 13 newly emerged ways to categorize MI attacks as a main focus on the *** order to address the second limitation,we provide-to our best knowledge-the first r

关键词： Machine learning Privacy and security Membershipinferenceattacks Defensivetechniques

基于内存增强自编码器的轻量级无人机网络异常检测模型

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通信学报 2024年第4期45卷 13-26页

作者：胡天柱沈玉龙任保全何吉刘成梁李洪钧西安电子科技大学网络与信息安全学院陕西西安710126 军事科学院系统工程研究院北京100070 西安电子科技大学计算机科学与技术学院陕西西安710126

为了解决传统智能攻击检测方法在无人机网络中存在的高能耗以及高度依赖人工标注数据的问题,提出一种基于双层内存增强自编码器集成架构的轻量级无人机网络在线异常检测模型。采用基于操作系统的消息队列进行数据包缓存,实现对高速数据... 详细信息

为了解决传统智能攻击检测方法在无人机网络中存在的高能耗以及高度依赖人工标注数据的问题,提出一种基于双层内存增强自编码器集成架构的轻量级无人机网络在线异常检测模型。采用基于操作系统的消息队列进行数据包缓存,实现对高速数据流的持久化处理,有效提升了模型的稳定性和可靠性。基于衰减窗口模型计算数据流复合统计特征,以增量更新方式降低了计算过程中的内存复杂度。利用层次聚类算法对复合统计特征进行划分,将分离的特征输入集成架构中的多个小型内存增强自编码器进行独立训练,降低了计算复杂度,同时解决了传统自编码器因重构效果过拟合而导致的漏报问题。在公开数据集和NS-3仿真数据集上的实验表明,所提模型在保证轻量级的同时,与基线方法相比,假阴性率分别平均降低了35.9%和48%。

关键词：无人机网络异常检测轻量级在线检测内存增强自编码器

Cooperative Secrecy to Resist Authentication Error in Two-Hop Wireless Networks

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Chinese Journal of Electronics 2016年第4期25卷 746-752页

作者： shen yulong PEI Qingqi MA Jianfeng School of Computer Science and Technology Xidian University The State Key Laboratory of Integrated Services Networks School of Telecommunications Engineering Xidian University

The authentication error in two-hop wireless networks is considered without knowledge of eavesdropper channels and *** wireless informationtheoretic security has attracted considerable attention recently.A prerequisite for available works is the precise distinction between legitimate nodes and *** it is unrealistic in the wireless *** is always existing in the node authentication *** of our knowledge,there are no works focus on solving this problem in the information-theoretic *** paper presents a eavesdropper model with authentication error and two eavesdropping ***,the number of eavesdroppers can be tolerated is analyzed while the desired secrecy is achieved with high probability in the limit of a large number of relay ***,we draw two conclusions for authentication error:1) The impersonate nodes are chosen as relay is the dominant factor of the transmitted message leakage,and the impersonation attack does seriously decrease the number of eavesdroppers can be tolerated.2) The error authentication to legitimate nodes is almost no effect on the number of eavesdroppers can be tolerated.

关键词： Wireless networks Information-theoretic security Cooperative secrecy Authentication error

Reversible Data Hiding Based on Pixel-Value-Ordering and Pixel Block Merging Strategy

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Computers, Materials & Continua 2019年第6期59卷 925-941页

作者： Wengui Su Xiang Wang yulong shen College of Computer Science and Technology Xidian UniversityXi’an710071China Guangxi Key Laboratory of Manufacturing System&Advanced Manufacturing Technology College of Mechanical EngineeringGuangxi UniversityNanning530004China College of Telecommunications Engineering Xidian UniversityXi’an710071China

With the reversible data hiding method based on pixel-value-ordering,data are embedded through the modification of the maximum and minimum values of a block.A significant relationship exists between the embedding performance and the block *** pixel-value-ordering methods utilize pixel blocks with a fixed size to embed data;the smaller the pixel blocks,greater is the embedding ***,it tends to result in the deterioration of the quality of the marked ***,a novel reversible data hiding method is proposed by incorporating a block merging strategy into Li et al.’s pixel-value-ordering method,which realizes the dynamic control of block size by considering the image ***,the cover image is divided into non-overlapping 2×2 pixel ***,according to their complexity,similarity and thresholds,these blocks are employed for data embedding through the pixel-value-ordering method directly or after being emerged into 2×4,4×2,or 4×4 sized ***,smaller blocks can be used in the smooth region to create a high embedding capacity and larger blocks in the texture region to maintain a high peak signal-to-noise *** results prove that the proposed method is superior to the other three advanced *** achieves a high embedding capacity while maintaining low distortion and improves the embedding performance of the pixel-value-ordering algorithm.

关键词： Reversible data hiding pixel-value-ordering prediction error expansion dynamic block partition

Structure-Based Genetic Analysis of Hel308a in the Hyperthermophilic Archaeon Sulfolobus islandicus

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Journal of Genetics and Genomics 2016年第6期43卷 405-413页

作者： Xueguo Song Jinfeng Ni yulong shen State Key Laboratory of Microbial Technology Shandong University

In archaea, the HEL308 homolog Hel308a（or Hjm） is implicated in stalled replication fork repair. The biochemical properties and structures of Hjm homologs are well documented, but in vivo mechanistic information is limited. Herein, a structure-based functional analysis of Hjm was performed in the genetically tractable hyperthermophilic archaeon, Sulfolobus islandicus. Results showed that domain V and residues within it, which affect Hjm activity and regulation, are essential and that the domain V-truncated mutants and sitedirected mutants within domain V cannot complement hjm chromosomal deletion. Chromosomal hjm deletion can be complemented by ectopic expression of hjm under the control of its native promoter but not an artificial arabinose promoter. Cellular Hjm levels are kept constant under ultraviolet（UV） and methyl methanesulfonate（MMS） treatment conditions in a strain carrying a plasmid to induce Hjm overexpression. These results suggest that Hjm expression and activity are tightly controlled, probably at the translational level.

关键词： Archaea Hel308a Replication fork regression Genetic complementation Sulfolobus islandicus

TAKAP: A Lightweight Three-Party Authenticated Key Agreement Protocol with User Anonymity

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Chinese Journal of Electronics 2021年第4期30卷 686-696页

作者： QIAO Hui DONG Xuewen shen yulong LIU Haozhe YANG Lingxiao School of Computer Science and Technology Xidian University Shaanxi Key Laboratory of Network and System Security Xidian University

The three-party authenticated key agreement protocol is a significant cryptographic mechanism for secure communication, which encourages two entities to authenticate each other and generate a shared session key with the assistance of a trusted party(remote server) via a public channel. Recently, Wang et al. put forward a three-party key agreement protocol with user anonymity and alleged that their protocol is able to resist all kinds of attacks and provide multifarious security features in Computer Engineering & Science, No.3, ***, we show that Wang et al.’s protocol is vulnerable to the password guessing attack and fails to satisfy user anonymity and perfect secrecy. To solve the aforementioned problems, a lightweight chaotic mapbased Three-party authenticated key agreement protocol(short for TAKAP) is proposed, which not only could provide privacy protection but also resist a wide variety of security attacks. Furthermore, it is formally proved under Burrows-Abadi-Needham(BAN) logic. Simultaneously,the performance analysis in this paper demonstrates that the proposed TAKAP protocol is more secure and efficient compared with other relevant protocols.

关键词： Three-party Authenticated key agreement Chaotic map Anonymity Security attacks

Analysis on the Resilience of Key Pre-distribution in Sensor Networks

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China Communications 2007年第3期4卷 30-37页

作者： shen yulong Ma Jianfeng Pei Qingqi Xidian Univ Sch Comp Sci & Technol Xian 710071 Shaanxi Peoples R China Minist Educ Key Lab Comp Networks & Informat Secur Xian 710071 Shaanxi Peoples R China

Resilience against node capture is one of the main indicators of the key pre-distribution security in sensor *** providing the attack model and the definition of the resilience against node capture of sensor networks,the resilience of basic random key pre-distribution,Q-composite random key pre-distribution and their reinforced schemes are analyzed and compared in *** results show that the size of key pool,the numbers of the keys stored in nodes and the value of Q determine the resilience of random key *** tradeoff between the resilience,security connectivity and costs in sensor networks is *** researches lay a foundation on the design of the secure protocol and the algorithm in the specific application environment of sensor networks.

关键词： sensor networks resilience analysis key pre-distribution

Distributed Information Flow Verification for Secure Service Composition in Smart Sensor Network

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China Communications 2016年第4期13卷 119-130页

作者： XI Ning SUN Cong MA Jianfeng CHEN Xiaofeng shen yulong Shaanxi Key Laboratory of Network and System Security XIDIAN University School of Cyber Engineering XIDIAN University

Accelerate processor, efficient software and pervasive connections provide sensor nodes with more powerful computation and storage ability, which can offer various services to user. Based on these atomic services, different sensor nodes can cooperate and compose with each other to complete more complicated tasks for user. However, because of the regional characteristic of sensor nodes, merging data with different sensitivities become a primary requirement to the composite services, and information flow security should be intensively considered during service composition. In order to mitigate the great cost caused by the complexity of modeling and the heavy load of single-node verification to the energy-limited sensor node, in this paper, we propose a new distributed verification framework to enforce information flow security on composite services of smart sensor network. We analyze the information flows in composite services and specify security constraints for each service participant. Then we propose an algorithm over the distributed verification framework involving each sensor node to participate in the composite service verification based on the security constraints. The experimental results indicate that our approach can reduce the cost of verification and provide a better load balance.

关键词： information flow security service composition formal verification smart sensor network