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Systems Microbiology and Biomanufacturing 2022年第4期2卷 643-652页

作者： Linli Yang Lukasz Peplowski yujuan shen Haiquan Yang Xianzhong Chen Wei shen Yuanyuan Xia Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan University1800 Lihu AvenueWuxi 214122JiangsuChina Institute of Physics Faculty of PhysicsAstronomy and InformaticsNicolaus Copernicus University in TorunGrudziadzka 587-100 TorunPoland

Sucrose phosphorylase(SPase)can transfer the glucosyl group of sucrose to different compounds and has been widely used in *** overcome the low thermostability of the sucrose phosphorylase from Leuconostoc mesenteroides ATCC 12291(LmSP),a method named PROSS was used to construct mutants with increased *** variants were screened by measuring their residual activities after heating at 50℃.Then,a single point mutant and a combined mutant with improved thermostability and activity were *** half-lives of mutants at 50℃ were approximately twice as high as those of the wild *** addition,2-O-α-d-glucosylglycerol(αGG)was synthesized by the wild type and the two improved variants,and the reaction conditions were *** the conditions of glycerol concentration of 3.2 mol/L,sucrose concentration of 1.2 mol/L,and enzyme concentration of 40 U/mL at 37℃ for 60 h,the yield ofαGG reached the maximum,and the sucrose conversion rate of the wild type,the mutant V23L and the combined mutant V23L/S424R were 62.3%,70.7%and 76.3%,*** this study,SPase mutants with higher activity and stability were obtained,and achieved high-level production ofαGG.

关键词： Sucrose phosphorylase PROSS Thermostability 2-O-α-d-Glucosylglycerol

Scandium and phosphorus co-doped perovskite oxides as high-performance electrocatalysts for the oxygen reduction reaction in an alkaline solution

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Journal of Materials Science & Technology 2020年第4期39卷 22-27页

作者： Meigui Xu Hainan Sun Wei Wang yujuan shen Wei Zhou Jun Wang Zhi-Gang Chen Zongping Shao State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing210009China Centre for Future Materials University of Southern QueenslandSpringfield CentralQLD4300Australia Materials Engineering The University of QueenslandBrisbaneQLD4072Australia WA School of Mines:Minerals Energy and Chemical Engineering(WASM-MECE)Curtin UniversityPerthWA6845Australia

The requirement for a sustainable and renewable energy has inspired substantial interests in designing and developing earth-abundant and high-effectiveness electrocatalysts/electrodes for fuel cells and metal-air batteries,in which oxygen reduction reaction(ORR)plays a crucial *** oxides have acquired rapid attention as ORR electrocatalysts to replace noble-metal-based catalysts owing to their intrinsic electrocatalytic activity,compositional and structural ***,we report a new Sc and P co-doped perovskite oxide(La0.8Sr0.2Mn0.95Sc0.025P0.025O3-δ,LSMSP)as an active and robust electrocatalyst for the ORR in an alkaline *** electrocatalyst shows superior ORR activity and stability than those of pristine La0.8Sr0.2MnO3-δ(LSM),Sc-doped LSM and P-doped LSM due to the optimized average valence of Mn ions,the large surface area,the smaller particle size and the synergetic effect introduced by the ***,compared to the benchmark Pt/C electrocatalyst,LSMSP electrocatalyst displays comparable ORR activity and superior *** above results suggest that the co-doping strategy of Sc and P into perovskites is a useful method to design high-performance electrocatalysts for the ORR,which can be used in other electrocatalysis-based applications.

关键词： Oxygen reduction reaction Perovskite oxide Co-doping Electrocatalyst Synergetic effect

Cyclospora cayetanensis infections among diarrheal outpatients in Shanghai： a retrospective case study

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Frontiers of Medicine 2018年第1期12卷 98-103页

作者： Yanyan Jiang Zhongying Yuan Guoqing Zang Dan Li Ying Wang Yi Zhang Hua Liu Jianping Cao yujuan shen National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention Key Laboratory of Parasite and VectorBiology Ministry of Health China WHO Collaborating Center for Tropical Diseases Shanghai 200025 China Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 200233 China

Cyclospora cayetanensis is a foodborne and waterborne pathogen that causes endemic and epidemic human diarrhea worldwide. A few epidemiological studies regarding C. cayetanensis infections in China have been conducted. During 2013, a total of 291 stool specimens were collected from patients with diarrhea at a hospital in urban Shanghai. C. cayetanensis was not detected in any of the stool specimens by traditional microscopy, whereas five stool specimens （1.72%, 5/291） were positive by PCR. These positive cases confirmed by molecular technology were all in the adult group （mean age 27.8 years; 2.94%, 5/170） with watery diarrhea. Marked infection occurred in the rainy season of May and July. Sequence and phylogenetic analyses of the partial 18S rRNA genes of C. cayetanensis isolated showed intra-species diversity of this parasite. This study showed, for the first time, that C. cayetanensis is a pathogen in outpatients with diarrhea in Shanghai, albeit at a low level. However, the transmission dynamics of this parasite in these patients remain uncertain.

关键词： Cyclospora cayetanensis outpatients with diarrhea stool specimens 18S rRNA gene

Intestinal Protozoan Infections in Patients with Diarrhea—Shanghai Municipality,Zhenjiang City,and Danyang City,China,2011–2015 and 2019–2021

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China CDC weekly 2022年第8期4卷 143-147页

作者： Yanyan Jiang Zhongying Yuan Hua Liu Jianhai Yin Yuan Qin Xiaofeng Jiang Jie Xu Jianping Cao yujuan shen National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)Key Laboratory of Parasite and Vector BiologyNational Health Commission of the People’s Republic of ChinaWorld Health Organization Collaborating Centre for Tropical DiseasesShanghaiChina School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina

Summary What is already known about this topic?Intestinal protozoa are common pathogens of diarrhea ***,the etiology of diarrhea due to intestinal protozoan infections in China is not *** is added by this report?Based on active syndromic surveillance in Shanghai,Zhenjiang,and Danyang during 2011–2015 and 2019–2021,89(1.67%)patients were infected with intestinal protozoa(Cryptosporidium spp.,Giardia duodenalis,Enterocytozoon bieneusi,and Cyclospora cayetanensis),and positivity rates statistically differed by region and *** are the implications for public health practice?This was the most comprehensive data collection in investigating parasitic diarrheal diseases in *** of these protozoa in diarrhea will provide new perspectives for detecting hidden etiological agents of diarrhea as early as possible.

关键词： globally ang detecting

The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

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Biomedical and Environmental Sciences 2011年第3期24卷 315-320页

作者： shen yujuan YIN JianHai YUAN ZhongYing LU WeiYuan XU YuXin XIAO LiHua CAO JianPing National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention Laboratory of Parasite and Vector Biology MOH China WHO Collaborating Centre for Malaria Schistosomiasis and Filariasis Shanghai 200025 China Centers for Disease Control and Prevention Atlanta Georgia USA

Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China. Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically （following modified acid fast staining）. In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed. Results The prevalence of Cryptosporidium in the collected samples was at 14.6% （31/213） and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype （Cryptosporidium ubiquiturn） demonstrating that this species was the primary sheep species found in sheep in China. Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.

关键词： Cryptosporidium ubiquitum Ovines Aba China Cryptosporidium cervine genotype

Cryptosporidiosis threat under climate change in China:prediction and validation of habitat suitability and outbreak risk for human-derived Cryptosporidium based on ecological niche models

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Infectious Diseases of Poverty 2023年第2期12卷 72-86页

作者： Xu Wang Yanyan Jiang Weiping Wu Xiaozhou He Zhenghuan Wang Yayi Guan Ning Xu Qilu Chen yujuan shen Jianping Cao National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)、Key Laboratory of Parasite and Vector BiologyNational Health Commission of the People’s Republic of China、World Health Organization Collaborating Center for Tropical DiseasesShanghai200025China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing102206China School of Life Sciences East China Normal UniversityShanghai200241China Key Laboratory of Public Health Safety Fudan UniversityMinistry of EducationFudan University Center for Tropical Disease ResearchFudan University School of Public HealthShanghai200031China The School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai200025China

Background Cryptosporidiosis is a zoonotic intestinal infectious disease caused by Cryptosporidium spp.,and its transmission is highly influenced by climate *** the present study,the potential spatial distribution of Cryptosporidium in China was predicted based on ecological niche models for cryptosporidiosis epidemic risk warning and prevention and *** The applicability of existing Cryptosporidium presence points in ENM analysis was investigated based on data from monitoring sites in 2011–*** occurrence data for China and neighboring countries were extracted and used to construct the ENMs,namely Maxent,Bioclim,Domain,and *** were evaluated based on Receiver Operating Characteristic curve,Kappa,and True Skill Statistic *** best model was constructed using Cryptosporidium data and climate variables during 1986‒2010,and used to analyze the effects of climate factors on Cryptosporidium *** climate variables for the period 2011‒2100 were projected to the simulation results to predict the ecological adaptability and potential distribution of Cryptosporidium in future in *** The Maxent model(AUC=0.95,maximum Kappa=0.91,maximum TSS=1.00)fit better than the other three models and was thus considered the best ENM for predicting Cryptosporidium habitat *** major suitable habitats for human-derived Cryptosporidium in China were located in some high-population density areas,especially in the middle and lower reaches of the Yangtze River,the lower reaches of the Yellow River,and the Huai and the Pearl River Basins(cloglog value of habitat suitability>0.9).Under future climate change,non-suitable habitats for Cryptosporidium will shrink,while highly suitable habitats will expand significantly(χ^(2)=76.641,P<0.01;χ^(2)=86.836,P<0.01),and the main changes will likely be concentrated in the northeastern,southwestern,and northwestern *** The Maxent model is applicable in predic

关键词： Cryptosporidium Cryptosporidiosis Ecological niche models Climate change One Health Maxent

Excretory-secretory products of helminthes directly promote the generation of B10 cells,but inhibited the generation of Th17 cells in vitro

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Excretory-secretory products of helminthes directly promote ...

微信阅读与纸质阅读的阅读效果比较——客观指标与主观感受的证据

中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Wei Pan Renxian Tang Chao Yan yujuan shen Jianping Cao Kuiyang Zheng Department of Pathogenic Biology and Immunology Laboratory of Infection and ImmunityXuzhou Medical College National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention

Background:Excretory-secretory products(ESPs) released by helminthes were well-known to regulate the T cell responses such as Th17/Tregs in infected hosts,however their roles in B cells functions remain *** this study,we systematically investigated the effects of ESPs of EgPSCs(the protoscoleces of Echinococcus granulosus) on the generation of effector subgroups of B and T cells in *** and major findings:Naive CD4 T cells and CD19 B cells were sorted from the spleens of normal Balb/c mice,and then respectively cultured in the presence of different concentrations of ***72 h,the supernatants and cells were collected to detect various cytokines using ELISA,and the frequencies of their subgroups using flow *** above indexes were also detected in the mice infecting with *** results were showed as follows:(i) The frequencies of Th17 cells were increased in infected mice,but ESPs could demolish their generation;(ii) ESPs directly promoted the induction of B10 cells,which was consistent to the results in infected *** results suggested that ESPs released by helminthes could directly promote the generation of B10 cells,but inhibit the frequencies of Th17 ***:We demonstrate that ESPs released by helminthes could directly regulate the generation of B10 while inhibiting Th17 cells,thereby combating the host inflammatory response to parasite infection.

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图书馆杂志 2021年第5期40卷 36-43页

作者：方瑜雯沈宇娟刘少英浙江理工大学心理系浙江杭州310018

微信阅读已成为一种普遍的阅读方式,但人们通常认为微信阅读深度不够,微信阅读能否成为辅助性学习方式仍存争议。为解决这一问题,本研究采用被试内设计,比较被试采用微信和纸质两种阅读方式完成说明文和论述文阅读的客观指标和主观感受... 详细信息

微信阅读已成为一种普遍的阅读方式,但人们通常认为微信阅读深度不够,微信阅读能否成为辅助性学习方式仍存争议。为解决这一问题,本研究采用被试内设计,比较被试采用微信和纸质两种阅读方式完成说明文和论述文阅读的客观指标和主观感受的差异。结果发现:被试在阅读时长、答题时长及阅读理解准确率三项客观指标上,纸质阅读和微信阅读差异不显著;被试更倾向于选择纸质阅读,认为微信阅读更容易出现跳读、沉浸感较差、浅阅读等主观感受;但被试的客观指标与主观感受之间并无相关。研究结果启示我们:人们对微信阅读的主观感受并不一定能完全反映微信阅读实际的学习效果,微信阅读可以作为一种辅助性的学习方式。

关键词：微信阅读纸质阅读阅读方式阅读感受

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幼儿社交-情绪发展与母亲育儿胜任感的关系

中国心理卫生杂志 2021年第8期35卷 631-636页

作者：沈宇娟徐婧雯刘少英浙江理工大学心理系杭州310018

目的:考察3~5岁幼儿社交-情绪发展水平,并探讨其与母亲育儿胜任感的关系。方法:选取375名3~5岁幼儿母亲,填写年龄与发育进程问卷;用社交-情绪(ASQ:SE-C)和中文版育儿胜任感量表(C-PSOC)评估幼儿的社交-情绪发展水平和母亲的育儿胜任感... 详细信息

目的:考察3~5岁幼儿社交-情绪发展水平,并探讨其与母亲育儿胜任感的关系。方法:选取375名3~5岁幼儿母亲,填写年龄与发育进程问卷;用社交-情绪(ASQ:SE-C)和中文版育儿胜任感量表(C-PSOC)评估幼儿的社交-情绪发展水平和母亲的育儿胜任感。结果:幼儿社交-情绪发展可疑迟缓检出137例(36.5%)。多重回归分析表明,幼儿自我调控与母亲育儿效能感、育儿满意度得分负向关联(β=-0.71、-0.96);幼儿沟通、人际互动与母亲育儿满意度得分负向关联(β=-0.46、-0.51)。结论:幼儿社交-情绪发展可疑迟缓人数比例偏高,幼儿自我调控、沟通、人际互动水平越低,母亲育儿胜任感越低。

关键词：幼儿社交-情绪发展母亲育儿胜任感

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北京:《红衣新娘》中被边缘化的一维乌托邦

外国语言与文化 2018年第2期2卷 56-63页

作者：申育娟北京外国语大学英语学院北京100089

英国当代作家露西·戈登的《红衣新娘》中女主人公戴利对北京的认识,可以从三个层面体现:去历史化的城市、乌托邦似的城市以及作为西方爱情故事中被边缘化的城市。通过对这三个方面呈现的"北京"形象进行细致的文本分析,探讨作者以此种... 详细信息

英国当代作家露西·戈登的《红衣新娘》中女主人公戴利对北京的认识,可以从三个层面体现:去历史化的城市、乌托邦似的城市以及作为西方爱情故事中被边缘化的城市。通过对这三个方面呈现的"北京"形象进行细致的文本分析,探讨作者以此种方式描写北京的原因。故事中的北京是作者投射的再现形象,是作为这一西方罗曼司中被边缘化的布景出现,而非一个让读者(尤其是中国读者)有真实感的北京。

关键词：北京形象去历史化边缘化乌托邦