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A polygalacturonase gene OsPG1 modulates water homeostasis in rice

The Crop Journal 2024年第1期12卷 79-91页

作者： Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang xihong shen Guanghua He Hong Wang Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China State Key Laboratory of Rice Biology and Breeding Key Laboratory for Zhejiang Super Rice ResearchChina National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 311401ZhejiangChina

A dynamic plant architecture is the basis of plant adaptation to changing *** many genes regulating leaf rolling have been identified,genes directly associated with water homeostasis are largely ***,we isolated a rice mutant,dynamic leaf rolling 1(dlr1),characterized by‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’during a sunny *** content was decreased in rolled leaves and water sprayed on leaves caused reopening,indicating that in vivo water deficiency induced the leaf ***-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1(OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1(PSL1)was responsible for the dynamic leaf rolling phenotype in the dlr1 ***1 encodes a polygalacturonase,one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell ***1 was constitutively expressed in various tissues and was enriched in *** of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density,leading to reduced transpiration and excessive water loss under specific conditions,but had normal root *** analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard *** findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling,providing insights for plants to adapt to environmental variation.

关键词： Photosynthesis Stomata Transpiration Leaf rolling

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A polygalacturonase gene OsPG1 modulates water homeostasis in rice

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The Crop Journal 2024年第1期

作者： Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang xihong shen Guanghua He Hong Wang

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Association Mapping of Quantitative Trait Loci for Grain Size in Introgression Line Derived from Oryza rufipogon

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Rice science 2020年第3期27卷 246-254页

作者： Kashif HUSSAIN ZHANG Yingxing Workie ANLEY Aamir RIAZ Adil ABBAS MdHasanuzzaman RANI WANG Hong shen xihong CAO Liyong CHENG Shihua Key Laboratory for Zhejiang Super Rice Research&State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Southern Rice Research Institute Thatta Agriculture Research WingGovernment of SindhSindh 73130Pakistan Department of Plant Sciences University of GondarP.O.Box 196Ethiopia Bangladesh Institute of Nuclear Agriculture Dhaka 2202Bangladesh

Grain size is one of the critical agronomic traits governing grain yield and quality in ***,the underlying genetic mechanisms that control grain size in rice are poorly *** used an introgression line derived from Zhonghui 8015 and Oryza rufipogon *** introgression line was evaluated under two different environmental conditions to dissect the quantitative trait loci controlling grain ***-wide association study(GWAS)was performed using 28193 SNPs through a general linear model,and 56 significant SNPs on different loci associated with the 4 grain size traits were *** genes including GS3 and q GL3 showed substantial effects on grain length and *** new stable loci were identified with pleiotropic effects on grain ***,gene expression analyses,combined gene-based associations,and functional annotations permitted the shortlisting of important dominant genes including GS3 and q GL3.

关键词： grain size association mapping Oryza sativa Oryza rufipogon quantitative trait locus genome-wide association analysis

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Use AMMI model to analyze adaptability of rice varieties

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Rice Science 1998年第4期 9-11页

作者： shen xihong WANG Lei YANG Shihua XIE Fuxian CNRRI,Hangzhou 310006,China

The adaptability of rice varieties is one of themost concerned questions to rice breeders. Inrice breeding, combined variety test and re-gional trials which included multiple genotypesand environments were conducted to identifythe adaptability of new rice varieties. In mostregional trials, interaction between genotypeand environment is significant, so it is impor-

关键词： AMMI Use AMMI model to analyze adaptability of rice varieties IPCA

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Use AMMI model to analyze adaptability of rice varieties

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Chinese Rice Research Newsletter 1998年第4期6卷 9-11页

作者： shen xihong, WANG Lei, YANG Shihua, and XIE Fuxian, CNRRI, Hangzhou 310006, China

The adaptability of rice varieties is one of themost concerned questions to rice *** breeding,combined variety test and re-gional trials which included multiple genotypesand environments were conducted to identifythe adaptability of new rice *** mostregional trials,interaction between genotypeand environment is significant,so it is impor-tant to analyze the interaction for estimating

关键词： AMMI Use AMMI model to analyze adaptability of rice varieties

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Development of Chromosome Segment Substitution Lines and Genetic Dissection of Grain Size Related Locus in Rice

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Rice science 2021年第4期28卷 322-324,I0012-I0017页

作者： Aamir Riaz Wang Huimin Zhang Zhenhua Workie Anley Zegeye Li Yanhui Wang Hong Xue Pao Peng Zequn shen Xi hong Cheng Shihua Zhang Yingxin State Key Laboratory of Rice Biology/National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006China Department of Plant Science University of GondarGondar 196Ethiopia

To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,*** line CSSL7 was selected for further mapping of qGL1.4/*** a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM11824 and *** F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and *** addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other *** summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.

关键词： breeding narrow bases

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Identification of QTLs involved for heading date with stable expression in paddy fields

Identification of QTLs involved for heading date with stable...

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中国作物学会2013年学术年会

作者： Huimin Wang Xiaoming Xu Yingxin Zhang Xiaodeng Zhan xihong shen Jianlin Wan Liyong Cao Shihua Cheng State Key Laboratory for Rice Biology China National Rice Research Institute Jiangxi Academy of Agriculture Sciences

Heading date(HD) is a critical trait for adaptation to different cultivation areas and cropping season and it is one of the leading objectives in rice *** QTLs for heading date were detected on chromosomes 1,2,4,5,6,7,8 and 10 in the past studied by using recombinant inbred lines(RILs) derived from a cross between Xieqingzao B(XB),a maintainer line with early heading,and R9308,a restore line with late *** qHD-7-1 was detected in four years,and it was mapped in interval RM5436-RM214 on chromosome 7,and it could explain phenotypic variance was 10.61%in 2006,3.91%in 2007,16.02%in 2010 and 18.75%in *** result showed qHD-7-1 could express stably in paddy *** identify and fine mapping the QTL,then it was backcrossed to BCF generation for measuring the *** qHD-7-1 was backcrossed to a near isogenic line(NIL) with a marker assisted selection(MAS) strategy for fine mapping. The qHD-7-1 was finally narrowed to a 418 kb interval(Indel 23-Indel 42).

关键词： rice(Oryza sativa L.) heading date QTL qHD-7-1

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Er掺杂对CeO_2(ZrO_2)/TiO_2催化剂脱硝性能的影响（英文）

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Chinese Journal of Catalysis 2016年第9期37卷 1521-1529页

作者：金奇杰沈岳松祝社民李喜红胡敏江苏先进无机功能复合材料协同创新中心南京工业大学材料科学与工程学院江苏南京210009 山东天璨环保科技有限公司山东淄博255086

氮氧化物(NO_x)是大气污染的主要因素之一,对其排放的治理成为较为迫切的需求.氨气选择性催化还原法(NH_3-SCR)是目前减少NO_x排放中应用最为广泛的技术.目前,商业SCR催化剂主要是V_2O_5(WO_3,MO_3)/TiO_2,但其具有活性温度窗口窄、N2... 详细信息

氮氧化物(NO_x)是大气污染的主要因素之一,对其排放的治理成为较为迫切的需求.氨气选择性催化还原法(NH_3-SCR)是目前减少NO_x排放中应用最为广泛的技术.目前,商业SCR催化剂主要是V_2O_5(WO_3,MO_3)/TiO_2,但其具有活性温度窗口窄、N2选择性低和对环境影响大等缺点.因此,新型的催化活性高且活性温度窗口宽的环境友好催化剂成为脱硝催化剂的研究热点.CeO_2因其独特的氧化还原性能和优异的储释氧能力在催化领域具有广泛应用,在NH_3-SCR中也研发出较多类型的铈基催化剂.我们课题组前期研发了具有优异脱硝性能的CeO_2(ZrO_2)/TiO_2催化剂,为拓展其应用范围,需要进行更深入的研究.理论上,Ti^(4+),Ce^(4+)以及Zr^(4+)离子的价态均高于Er^(3+),且离子半径相近.换言之,Er_2O_3能够与TiO_2以及CeO_2产生缺陷反应增大催化剂的缺陷浓度,进而提高催化剂的催化活性.本文以溶胶-凝胶法制备了一系列Er掺杂CeO_2(ZrO_2)/TiO_2催化剂,测试了样品的NH_3-SCR催化活性和N2选择性,并且在320°C下连续24 h测试了水蒸气、SO_2以及两者混合作用对催化剂活性的影响.使用X射线衍射(XRD)、N2等温吸附-脱附(N2-BET)、NH_3程序升温脱附(NH_3-TPD)、H2程序升温还原(H2-TPR)、光致发光光谱(PL)、电子顺磁共振(EPR)以及X射线光电子能谱(XPS)对催化剂进行了表征.XRD结果显示,Er掺杂后催化剂的结晶程度降低,且图谱中没有出现明显的Er_2O_3衍射峰,即Er在催化剂上有较好的分散度且掺杂抑制了催化剂的晶化.NH_3-TPD和H2-TPR结果表明,Er掺杂降低了酸强且提高了储释氧能力,催化剂的氧化还原能力则有所减弱.PL和EPR测试结果显示,掺杂后的催化剂氧空位浓度和Ti^(3+)浓度有所增加,与前期理论设计一致.XPS测试结果表明,掺入Er后催化剂的化学吸附氧含量和Ti^(3+)浓度增加,Ce^(3+)浓度基本不变,推测是CeO_2(ZrO_2)/TiO_2催化剂中掺入的Er主要与载体TiO_2,而不是与活性组分CeO_2或助剂ZrO_2产生缺陷反应的结果.CeO_2(ZrO_2)/TiO_2催化剂最高活性为94.28%,其活性温度窗口为230–390°C,掺入Er(Er:Ce=0.10:1)后,催化剂的整体活性尤其是350°C以下的催化活性具有明显提升,最高活性达到98.85%,活性温度窗口也拓展为220–395°C.单独的水蒸气对催化活性影响很小,SO_2会部分降低催化剂活性,而当两者混合作用时,催化剂活性下降最为显著,且Er掺杂后CeO_2(ZrO_2)/TiO_2催化剂的抗中毒能力有所增强.Er掺杂CeO_2(ZrO_2)/TiO_2催化剂显示出较好的抗硫抗水中毒能力以及较高的NH_3-SCR催化活性和N2选择性,应该是一种具有应用前景的SCR催化剂.Er掺杂降低了催化剂的酸强,抑制了TiO_2和铈锆固溶体的晶化,提高了Ti^(3+)和氧空位浓度并增强了储释氧能力,是CeO_2(ZrO_2)/TiO_2催化剂活性提高的主要原因.

关键词： CeO2(ZrO2)/TiO2 铒掺杂选择性催化还原氮氧化物催化性能

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