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Improved Isolation and Culture of Embryonic Germ Cells from Guanzhong Dairy Goat

Agricultural Sciences in China 2006年第7期5卷 550-557页

作者： YANG Wei-feng GE Xiu-guo HUA Jin-lian shen Wen-zheng DOU Zhong-ying Shaanxi Center of Stem Cells Engineering ＆ Technology Northwest A ＆ F University Yangling 712100 P.R.China

A total of 219 embryonic-germ-cell-like （EG-like） clumps were derived from 15 selected goat fetuses. Isolation of primordial germ cells （PGCs） based on co-culture with primary goat embryonic fibroblast showed no difference from traditional feeder layer-based culture method used in mouse and human. The putative primary EG colonies were multilayer clumps of compact cells with unclear cell-cell boundaries. Three subculture methods of goat EG-like colony, traditional enzymatic digestion, mechanical cutting and combination of the both, were compared in this study. As a result, EG-like colonies traditionally disassociated with collagenase 1V could be subcultured for up to 4 passages. And the mechanically disaggregated EG-like colonies were successfully maintained 9-12 passages with or without enzymatic treatment. The pluripotency of the EG-like colonies was identified by their specific marker staining, spontaneous differentiation and embryoid bodies （EBs） formation in vitro. Most goat EG-like colonies （〉 80%） were AKP positive and immunocytochemically characterized with positive SSEA-1, Oct-4 and c-kit staining but SSEA-4. Under the condition of delaying passage, goat EG-like cells could differentiate into fibroblast-like, epithelium-like, and neuron-like cells. In addition, EBs could be obtained successfully in routine hanging drop culture. The serum free culture system （feeder layer-based） used in this study was suitable for keeping PGCs and EG-like cells in their undifferentiated condition, but failed to converse them to immortal cells. These results indicated that mechanical cutting is an effective method for passaging goat EG cell colonies. However, the microenvironment of conversing EG cells to immortal cells is still unclear.

关键词： goat primordial germ cells embryonic germ cells co-culture mechanical cutting

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GIT1 enhances neurite outgrowth by stimulating microtubule assembly

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Neural Regeneration Research 2016年第3期11卷 427-434页

作者： Yi-sheng Li Li-xia Qin Jie Liu Wei-liang Xia Jian-ping Li Hai-lian shen Wei-Qiang Gao State Key Laboratory of Oncogenes and Related Genes Renji-Med X Stem Cell Research CenterRen Ji HospitalSchool of Biomedical Engineering & Med-X Research InstituteShanghai Jiao Tong University Department of Neurology Shanghai Renji Hospital Collarative Innovation Center of Systems Biomedicine Shanghai Jiao Tong University

GIT1,a G-protein-coupled receptor kinase interacting protein,has been reported to be involved in neurite ***,the neurobiological functions of the protein remain *** this study,we found that GIT1 was highly expressed in the nervous system,and its expression was maintained throughout all stages of neuritogenesis in the *** primary cultured mouse hippocampal neurons from GIT1 knockout mice,there was a significant reduction in total neurite length per neuron,as well as in the average length of axon-like structures,which could not be prevented by nerve growth factor *** of GIT1 significantly promoted axon growth and fully rescued the axon outgrowth defect in the primary hippocampal neuron cultures from GIT1 knockout *** GIT1 N terminal region,including the ADP ribosylation factor-GTPase activating protein domain,the ankyrin domains and the Spa2 homology domain,were sufficient to enhance axonal ***,GIT1 bound to many tubulin proteins and microtubule-associated proteins,and it accelerated microtubule assembly in ***,our findings suggest that GIT1 promotes neurite outgrowth,at least partially by stimulating microtubule *** study provides new insight into the cellular and molecular pathogenesis of GIT1-associated neurological diseases.

关键词： nerve regeneration GIT1 hippocampal neurons neurite outgrowth tubulin microtubule-associated proteins neural regeneration

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Separating cyclostationary signals from spectrally overlapping interference

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Frontiers of Electrical and Electronic Engineering in China 2006年第3期1卷 307-312页

作者： GUO Jie LIU Yun YE Zhi-hui SONG Tie-cheng shen lian-feng National Mobile Communications Research Laboratory Southeast UniversityNanjing 210096China

This paper studies an algorithm about separating spectrally overlapping signals using the cyclostationary properties of *** the basis of direct sequence spread system(DSSS),frequency shift filter is added into the re-ceiver of the communication *** the structure of frequency shift filter is more complicated than the time-domain filter,it uses both time correlations and fre-quency spectrum correlations so it can achieve better per-formances on separating the overlapping *** the analysis of cyclostationary characteristic and frequency spec-trum correlation,the structure of the frequency shift filter can be ***,a self-adaptive algorithm is utilized for the purpose of achieving optimum multidimensional tap weights of frequency shift *** simulation results indi-cate that this method can efficiently separate overlapping signals,and its error rate is lower than the time-domain filter or DSSS system by two orders of magnitude on the condition that high-power interference is added into the system.

关键词： cyclostationary frequency shift filter overlap signal spectral correlation

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A Flier-plate Material with Graded Impedance Used in Dynamic High-pressure Physics

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Journal of Wuhan University of Technology(Materials Science) 2002年第4期17卷 32-35页

作者：王传彬 State Key Lab of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan China

By using such flier-plate material,quasi-isentropic compression can be *** on it,hypervelocity launching is further *** a result,an extremely high dynamic pressure can be obtained in laboratory,offering a practical method for the comprehensive determination of materials behavior,response,equation-of-state and properties in dynamic loading process.

关键词： flier-plate material with graded impedance quasi-isentropic compression hypervelocity launching

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Spiral Field Generation in Smith-Purcell Radiation by Helical Metagratings

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Research 2019年第1期2019卷 1115-1122页

作者： Liqiao Jing Zuojia Wang Xiao Lin Bin Zheng Su Xu lian shen Yihao Yang Fei Gao Min Chen Hongsheng Chen Key Lab.of Advanced Micro/Nano Electronic Devices&Smart Systems of Zhejiang College of Information Science and Electronic EngineeringZhejiang UniversityHangzhou 310027China Department of Physics Massachusetts Institute of TechnologyCambridgeMA 02139USA School of Information Science and Engineering Shandong UniversityQingdao 266237China Division of Physics and Applied Physics School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012China

Moving electrons interacting with media can give rise to electromagnetic radiations and has been emerged as a promising platform for particle detection,spectroscopies,and free-electron *** this letter,we investigate the Smith-Purcell radiation from helical metagratings,chiral structures similar to deoxyribonucleic acid(DNA),in order to understand the interplay between electrons,photons,and object *** feld patterns can be generated while introducing a gradient azimuthal phase distribution to the induced electric dipole array at the cylindrical *** measurements show efcient control over angular momentum of the radiated feld at microwave regime,utilizing a phased electromagnetic dipole array to mimic moving charged *** angular momentum of the radiated wave is determined solely by the handedness of the helical structure,and it thus serves as a potential candidate for the detection of chiral *** fndings not only pave a way for design of orbital angular momentum free-electron lasers but also provide a platform to study the interplay between swif electrons with chiral objects.

关键词： momentum chiral angular

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云南省2013-2022年登革热流行趋势及暴发疫情特征分析

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中国媒介生物学及控制杂志 2024年第1期35卷 63-68,73页

作者：陈莉华念鹏英沈秀莲郑尔达段江丽云南省疾病预防控制中心疫情监测/突发公共卫生事件处置中心云南昆明650022 玉溪市江川区疾病预防控制中心疾病控制科云南玉溪653100 大理白族自治州疾病预防控制中心质量管理室云南大理671000

目的分析云南省登革热流行特征系统及暴发疫情情况,为制定有效防控措施提供科学依据。方法从“中国疾病预防控制信息系统”的“传染病监测”和“突发公共卫生事件管理信息系统”模块收集2013-2022年云南省报告的登革热病例及暴发疫情... 详细信息

目的分析云南省登革热流行特征系统及暴发疫情情况,为制定有效防控措施提供科学依据。方法从“中国疾病预防控制信息系统”的“传染病监测”和“突发公共卫生事件管理信息系统”模块收集2013-2022年云南省报告的登革热病例及暴发疫情相关信息,采用集中度法分析季节性,使用Excel 2007软件进行数据整理,MapInfo 7.8软件绘制地图,采用SPSS 26.0统计软件进行相关性分析,率或构成比的比较采用χ^(2)检验,采用描述流行病学方法对登革热流行特征及暴发疫情处置情况进行分析。结果 2013-2022年云南省共报告登革热病例16 774例,其中本地病例11 944例,境外输入病例4 521例,境内输入病例309例,年均报告发病率为3.53/10万。发病呈现隔年高发的现象,发病集中度(M)为0.84,具有很强的季节性。输入病例主要发生在6-10月,本地病例以8-11月为主。输入病例中男女性别比略高于本地病例(χ^(2)=37.596,P<0.001)。本地病例以30~<40岁人群为主(20.14%),输入病例以20~<30岁人群最多(27.58%)。本地病例出现在边境5个州(市),输入病例中境外主要来源于东南亚和非洲国家,缅甸、柬埔寨和老挝是主要来源国(96.81%),境内以西双版纳傣族自治州为主(85.76%)。病例职业主要为农民、商业服务、干部/离退人员、家务及待业和学生,本地病例中以商业服务者构成比最高(21.89%),输入病例中以农民构成比最高(39.90%),输入病例与本地病例的职业分布差异有统计学意义(χ^(2)=1 535.415,P<0.001)。2013-2022年共报告登革热暴发疫情28起,其中较大事件1起,一般事件27起,报告病例数占总病例数的83.41%,每起事件平均涉及病例499.71例;首发病例发病到核实事件发生中位时间为7(5,9) d,暴发疫情持续时间中位数为110(27,140) d;25起暴发事件发生在村(社区)。结论云南省登革热存在明显季节、人群以及地区分布规律,应进一步加强输入病例管控、媒介监测控制以及重点地区的防控工作。

关键词：登革热流行特征暴发本地病例输入病例

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Design of High Performance MXene/Oxide Structure Memristors for Image Recognition Applications

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Chinese Journal of Electronics 2024年第2期33卷 336-345页

作者： Xiaojuan lian Yuelin SHI Xinyi shen Xiang WAN Zhikuang CAI Lei WANG The College of Integrated Circuit Science and Engineering Nanjing University of Posts and Telecommunications The National and Local Joint Engineering Laboratory of RF Integration and Micro-Assembly Technology Nanjing University of Posts and Telecommunications

Recent popularity to realize image recognition by memristor-based neural network hardware systems has been witnessed owing to their similarities to neurons and ***,the stochastic formation of conductive filaments inside the oxide memristor devices inevitably makes them face some drawbacks,represented by relatively higher power consumption and severer resistance switching *** this work,we design and fabricate the Ag/MXene（Ti3C2）/SiO2/Pt memristor after considering the stronger interactions between Ti3C2and Ag ions,which lead to a Ti3C2/SiO2structure memristor owning to much lower "SET" voltage and smaller resistance switching fluctuation than pure ***,the conductances of the Ag/Ti3C2/SiO2/Pt memristor have been modulated by changing the number of the applied programming pulse,and two typical biological behaviors,i.e.,longterm potentiation and long-term depression,have been ***,device conductances are introduced into an integrated device-to-algorithm framework as synaptic weights,by which the MNIST hand-written digits are recognized with accuracy up to 77.39%.

关键词： Performance evaluation Silicon compounds Image recognition Power demand Memristors Switches Programming

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CFE下FRESH滤波器性能分析及CFE校正

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电子与信息学报 2008年第4期30卷 853-858页

作者：魏安全沈连丰东南大学移动通信国家重点实验室

频移(FRESH)滤波器的研究通常都基于接收机准确知道信号或干扰循环频率(CF)的假设,但在FRESH滤波器的实际应用环境中,循环频率误差(CFE)往往不可避免地存在。该文基于FRESH滤波器抑制DSSS/BPSK系统窄带干扰的模型,分析了CFE对FRESH滤波... 详细信息

频移(FRESH)滤波器的研究通常都基于接收机准确知道信号或干扰循环频率(CF)的假设,但在FRESH滤波器的实际应用环境中,循环频率误差(CFE)往往不可避免地存在。该文基于FRESH滤波器抑制DSSS/BPSK系统窄带干扰的模型,分析了CFE对FRESH滤波器抽头系数、估计误差和系统误码性能的影响,提出了一种改进的CFE校正算法。理论分析与数值仿真的结果表明,FRESH滤波器的性能对CFE十分敏感,该文提出的算法能有效地校正CFE,使FRESH滤波器在CFE下仍能保持稳健而优良的性能。

关键词：频移滤波器循环平稳谱相关循环频率误差干扰抑制

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基于沉浸边界法的轴流泵内鱼体运动数值模拟

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水力发电学报 2021年第7期40卷 95-104页

作者：杨丹丹赵明翔叶维祥 shen lian 罗先武清华大学能源与动力工程系北京100084 明尼苏达大学机械工程系美国明尼阿波利斯55455

为精细分析鱼类在水力机械流道中的运动特性,本文以轴流泵为例,采用沉浸边界(immersed boundary,IB)与流固耦合(fluid-structure interaction,FSI)及大涡模拟(large eddy simulation,LES)相结合的方法,对泵内流场及鱼体通过泵流道的运... 详细信息

为精细分析鱼类在水力机械流道中的运动特性,本文以轴流泵为例,采用沉浸边界(immersed boundary,IB)与流固耦合(fluid-structure interaction,FSI)及大涡模拟(large eddy simulation,LES)相结合的方法,对泵内流场及鱼体通过泵流道的运动过程进行了数值模拟。结果表明,在相同雷诺数下计算得到的设计工况扬程系数与实验值误差仅为2.7%,验证了IB-FSI-LES方法适合于模拟轴流泵内部流动。与将鱼体视为流体示踪粒子的常规计算相比,采用IB-FSI-LES方法可以得到鱼体在泵内的复杂运动轨迹,捕捉鱼体与叶片发生碰撞时的运动突变以及叶轮内鱼体周围流场中涡结构的形成和脱落。研究结果可为水利水电工程中鱼类过机损伤精确评估及鱼类友好型水力机械优化设计提供科学依据。

关键词：水力机械沉浸边界法流固耦合鱼体运动鱼类友好

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周期序列DSSS系统抗CW干扰性能分析

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通信学报 2007年第5期28卷 1-6页

作者：魏安全沈连丰东南大学移动通信国家重点实验室

在简要论述按传统的基于标准高斯近似(SGA)假设得到的DSSS系统抗单音连续波(CW)干扰性能分析结果的基础上,推导出CW干扰经周期序列DSSS系统相关解扩处理产生的符号判决变量中干扰分量的数学表达式,对周期序列DSSS系统抗CW干扰的实际性... 详细信息

在简要论述按传统的基于标准高斯近似(SGA)假设得到的DSSS系统抗单音连续波(CW)干扰性能分析结果的基础上,推导出CW干扰经周期序列DSSS系统相关解扩处理产生的符号判决变量中干扰分量的数学表达式,对周期序列DSSS系统抗CW干扰的实际性能进行了理论分析与仿真。结果表明,周期序列DSSS系统在CW干扰下符号判决变量中的干扰分量呈现固定电平或单频波动,并不近似于标准高斯分布,周期序列DSSS系统抗CW干扰的实际性能与基于SGA假设的分析结果完全不同,其误码性能不仅与干信比、干扰频偏和相位密切相关,还与具体的扩频序列有关。

关键词：直接序列扩频性能分析周期序列连续波干扰标准高斯近似

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