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Diagnostic description and geographic distribution of four new cryptic species of the blue-spotted maskray species complex(Myliobatoidei:Dasyatidae;Neotrygon spp.)based on DNA sequences

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Journal of oceanology and Limnology 2018年第3期36卷 827-841页

作者： Philippe BORSA Irma S.ARLYZA Thierry B.HOAREAU Kang-Ning shen Institut de Recherche pour le Développement(IRD) UMR 250"Ecologie Marine Tropicale des Oceans Pacifique et Indien"BP A598848 NouméaNew Caledonia Lembaga Ilmu Pengetahuan Indonesia(LIPI) Pusat Penelitian Oseanografi(P20)JakartaIndonesia Molecular Ecology and Evolution Programme Department of GeneticsUniversity ofPretoriaPretoriaSouth Africa Aquatic Technology Laboratories Agricultural Technology Research InstituteTaiwanChina

Nine morphologically similar but genetically distinct lineages in the blue-spotted maskray species complex, previously Neotrygon kuhlii(Müller and Henle) qualify as cryptic species. Four of these lineages have been previously described as N eotrygon australiae Last, White and Séret, N eotrygon caeruleopunctata Last, White and Séret, N eotrygon orientale Last, White and Séret, and N eotrygon varidens(Garman), but the morphological characters used in the descriptions off ered poor diagnoses and their geographic distributions were not delineated precisely. The objective of the present work is to complete the description of the cryptic species in the complex. Here, an additional four lineages are described as new species on the basis of their mitochondrial DNA sequences: Neotrygon bobwardi, whose distribution extends from the northern tip of Aceh to the western coast of Sumatera; N eotrygon malaccensis, sampled from the eastern part of the Andaman sea and from the Malacca Strait; N eotrygon moluccensis, from the eastern half of the Banda sea; and Neotrygon westpapuensis from the central portion of northern West Papua. The geographic distributions of N. australiae, N. coeruleopunctata, N. orientale, and N. varidens are updated. For each species, a diagnosis is provided in the form of a combination of private or partly-private nucleotides at 2–4 nucleotide sites along a 519-base pair fragment of the CO1 gene. We believe that the present taxonomic revision will provide information relevant to the sound management and conservation of cryptic species of the blue-spotted maskray in the Coral Triangle region.

关键词： molecular taxonomy diagnosis distribution

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Dietary preferences and potential ecological impact on the zooplankton community of Nemopilema nomurai based on stable isotope and fatty acid analyses

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Journal of oceanology and Limnology 2022年第3期40卷 1085-1096页

作者： Junjian wang Chaolun LI Guang YANG Zhencheng TAO Yanqing wang Haochen XIAN Key Laboratory of marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China North china sea marine Forecasting Center of State Oceanic Administration Qingdao 266100China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China department of Engineering and Technology institute of oceanology Chinese Academy of SciencesQingdao 266071China

Information on the dietary composition and food preferences of the giant jellyfish Nemopilema nomurai is important for understanding the trophic drivers of jellyfish outbreaks and their ecological *** used fatty acid(FA)and stable isotope(SI)biomarkers to analyze the diet of *** from the Yellow sea in August *** was found at all sampling stations,with abundances ranging from 59 inds./km^(2) to 1651 inds./km^(2).There were no significant differences between large(>80 cm in diameter)and small(20–30 cm in diameter)medusae,either in FA compositions or in SI values,which suggests that large and small jellyfi sh have the same food composition and similar trophic *** to other zooplanktons,the relatively high levels of C20꞉4n-6 in total FAs(~12%)indicates that organic detritus contributes considerably to the food composition of the *** mixed model Stable Isotope Analysis in R(SIAR)revealed that *** tended to prey on smaller organisms(1000μm in diameter)which comprised about 70%of its *** means the *** blooms will put high feeding pressure on the small *** similar SI values and FA composition indicates that krill may share the same food resources with ***,which suggests that the jellyfi sh blooms may affect krill populations as a result of food competition.

关键词： Nemopilema nomurai fatty acid stable isotope dietary preferences

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The spring Yellow sea fog：synoptic and air–sea characteristics associated with different airflow paths

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Acta Oceanologica Sinica 2018年第1期37卷 20-29页

作者： HUANG Jian wang Bin wang Xin HUANG Fei LU Weihua Tu Jing institute of Tropical and marine Meteorology China Meteorological Administration Guangzhou 510080 China department of Meteorology University of Hawaii Honolulu HI 96822 USA south china sea institute of oceanology Chinese Academy of Sciences Guangzhou 510301 China Physical Oceanography Laboratory Ocean University of China Qingdao 266100 China

The fog occurs frequently over the Yellow sea in spring（April–May）, a climatical period of Asian monsoon transition. A comprehensive survey of the characteristic weather pattern and the air-sea condition is provided associated with the fog for the period of 1960–2006. The sea fog is categorized by airflow pathways of backward trajectory cluster analysis with the surface observations derived from international comprehensive oceanatmosphere dataset（I_COADS） I_COADS datasets and contemporaneous wind fields from the National Centers for Environmental Prediction（NCEP）/National Center for Atmospheric Research（NCAR） reanalysis. On the basis of the airflow paths, the large-scale lower-tropospheric circulation patterns and the associated surface divergence,the distribution of a vertical humidity, the horizontal water vapor transportation and the air-sea temperature difference are investigated and the major findings are summarized as follows.（1） Four primary clusters of the airflow paths that lead to spring sea fog formation are identified. They are originated from the northwest, east,southeast and southwest of the Yellow sea, respectively.（2） Springtime Yellow sea fog occurs under two typical weather patterns： the Yellow sea high（YSH） and cyclone and anticyclone couplet（CAC）. Each pattern appears by about equal chance in April but the YSH occurrence drops to around one third and the CAC rises to around two third of chance in May.（3） The common feature in the two types of synoptic conditions is that surface divergence center is located over the Yellow sea.（4） For the YSH type of fog, water vapor comes mainly from local evaporation with a well-defined dry layer present in the lower atmosphere; for the CAC type of fog, however, water vapor comes mainly from areas outside the Yellow sea with a thick surface layer of high humidity.（5） With the differences in weather patterns and its associated vertical distribution of the humidity and the transportation of water vapor, there are

关键词： springtime sea fog Yellow sea airflow clusters synoptic patterns characteristics of sea fog

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Spatial assessment of water quality using chemometrics in the Pearl River Estuary, china

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Frontiers of Earth Science 2017年第1期11卷 114-126页

作者： Meilin WU Youshao wang Junde DONG Fulin SUN Yutu wang Yiguo HONG State Key Laboratory of Tropical Oceanography South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China marine Biology Research Station at Daya Bay Chinese Academy of Sciences Shenzhen 518121 China

A cruise was commissioned in the summer of 2009 to evaluate water quality in the Pearl River Estuary （PRE）. Chemometrics such as Principal Component Analysis （PCA）, Cluster analysis （CA） and Self-Organiz- ing Map （SOM） were employed to identify anthropogenic and natural influences on estuary water quality. The scores of stations in the surface layer in the first principal component （PC1） were related to NH4-N, PO4-P, NO2-N, NO3-N, TP, and Chlorophyll a while salinity, turbidity, and SiO3-Si in the second principal component （PC2）. Similarly, the scores of stations in the bottom layers in PC1 were related to PO4-P, NO2-N, NO3-N, and TP, while salinity, Chlorophyll a, NH4-N, and SiO3-Si in PC2. Results of the PCA identified the spatial distribution of the surface and bottom water quality, namely the Guangzhou urban reach, Middle reach, and Lower reach of the estuary. Both cluster analysis and PCA produced the similar results. Self-organizing map delineated the Guangzhou urban reach of the Pearl River that was mainly influenced by human activities. The middle and lower reaches of the PRE were mainly influenced by the waters in the south china sea. The information extracted by PCA, CA, and SOM would be very useful to regional agencies in developing a strategy to carry out scientific plans for resource use based on marine system functions.

关键词： principal component analysis self-organizing map estuarine water quality the Pearl River Estuary spatial variation

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Factors aff ecting lipid and fatty acid composition of Calanus sinicus in the Yellow sea and the East china sea in spring

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Journal of oceanology and Limnology 2022年第1期40卷 173-182页

作者： Yanqing wang Zhencheng TAO Chaolun LI Mengtan LIU Key Laboratory of marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Engineering and Technology department Institute of OceanologyChinese Academy of SciencesQingdao 266071China Jiaozhou Bay National marine Ecosystem Research Station Chinese Academy of SciencesQingdao 266071China Center of Deep sea Research Institute of OceanologyChinese Academy of SciencesQingdao 266071China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China

The factors aff ecting lipid and fatty acid composition of copepod Calanus sinicus in the Yellow sea(YS)and the East china sea(ECS)were examined in this *** spring,there were signifi cant diff erences between these two regions for both environmental conditions and food *** regional diff erence signifi cantly infl uenced the lipid and fatty acid profi les of *** results show that *** has a higher lipids content in ECS,especially for wax ester and triglyceride lipids,indicating a more active and effi cient *** to BIO-ENV analysis,the variation of lipids profi les may be infl uenced majorly by water ***,the fatty acids(FAs)profi les of *** were also diff erent between YS and ECS,especially in the four major contributors,C22.1ω11,eicosapentaenoic acid(EPA),docosahexenoic acid(DHA),and C20꞉1ω*** considerable amounts of self-biosynthesized FAs of herbivorous copepod(C22.1ω11 and C20꞉1ω9)and low DHA/EPA ratio may indicate that *** in ECS feed mainly on phytoplankton comparing to those in *** fatty acid profi les of *** were aff ected by the diff erences in food availability.

关键词： Calanus sinicus lipid storage wax ester fatty acid composition Yellow sea and East china sea

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Asynchronous marine-terrestrial signals of the last deglacial warming in East Asia associated with low- and high-latitude climate changes

Asynchronous marine-terrestrial signals of the last deglacia...

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中国科学院地质与地球物理研究所2.13年度（第13届）学术年会

作者： Deke Xu Houyuan Lu Naiqin Wu Zhenxia Liu Tiegang Li Caiming shen Luo wang Key Laboratory of Cenozoic geology and Environment Institute of Geology and GeophysicsChinese Academy of Sciences First institute of Oceanography State Oceanic Administration Key Laboratory of marine Science and Numerical Modeling State Oceanic Administration institute of oceanology Chinese Academy of Sciences Key Laboratory of Plateau Lake Ecology and Global Change Yunnan Normal University

A high-resolution multiproxy record,including pollen,foraminifera, and alkenone paleothermometry,obtained from a single core (DG9603) from the Okinawa Trough,East china sea(ECS),provided unambiguous evidence for asynchronous climate change between the land and ocean over the past 40 *** land,the deglacial stage was characterized by rapid warming,as reflected by paleovegetation, and it began ca.15 kaBP,consistent with the timing of the last deglacial warming in ***,sea surface temperature estimates from foraminifera and alkenone paleothermometry increased around 20-19 kaBP,as in the Western Pacific Warm Pool (WPWP).sea surface temperatures in the Okinawa Trough were influenced mainly by heat transport from the tropical western Pacific Ocean by the Kuroshio Current,but the epicontinental vegetation of the ECS was influenced by atmospheric circulation linked to the northern high-latitude *** terrestrial and marine signals of the last deglacial warming in East Asia were thus clearly related to ocean currents and atmospheric *** argue that (/) early warming seawater of the WPWP,driven by low-latitude insolation and trade winds,moved northward via the Kuroshio Current and triggered marine warming along the ECS around 20-19 kaBP similar to that in the WPWP,and(ii) an almost complete shutdown of the Atlantic Meridional Overturning Circulation ca.18-15 kaBP was associated with cold Heinrich stadial-1 and delayed terrestrial warming during the last deglacial warming until ca.15 kaBP at northern high latitudes,and hence in East *** deglacial warming therefore lagged behind marine changes by ca.3-4 ka.

关键词： asynchrony East Asian monsoon land-sea correlation low- and high-latitude interplay thermohaline circulation

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上地幔含水量对海底扩张过程中洋壳厚度的影响:数值模拟

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地球科学 2021年第3期46卷 826-839页

作者：邵佳许鹤华谌永强施小斌王晓芳中国科学院边缘海与大洋地质重点实验室南海海洋研究所南海生态环境工程创新研究院广东广州511458 南方海洋科学与工程广东省实验室(广州) 广东广州511458 中国科学院大学北京100049

地幔中不同含量的水会对洋壳的生成产生重要影响,但目前不同含水量下的均匀和局部含水地幔会怎样影响洋壳厚度还不清楚.利用动力学数值模拟的方法,对上地幔均匀含水和局部含水两种情况下洋壳的生成过程展开研究.结果表明:当上地幔均匀... 详细信息

地幔中不同含量的水会对洋壳的生成产生重要影响,但目前不同含水量下的均匀和局部含水地幔会怎样影响洋壳厚度还不清楚.利用动力学数值模拟的方法,对上地幔均匀含水和局部含水两种情况下洋壳的生成过程展开研究.结果表明:当上地幔均匀含水时,含水量的增加在减小最大熔融分数的同时,会增大初始熔融深度和熔融面积,因而生成的洋壳厚度会增加.当上地幔局部含水时,局部含水地幔熔融后也会增大生成的洋壳厚度,但开始影响洋壳厚度的时间与其含水量有关.结合南海洋壳特征进一步分析认为:南海扩张期间其地幔源含水量具有非均质性.东部次海盆的洋壳比西南次海盆厚1 km,可能是因为前者地幔源含水量整体高于后者(本模型表明约高50×10^(-6)).南海玄武岩中碳酸盐化硅酸盐熔体的存在,可能是由局部高含水量地幔在深部熔融产生的熔体携带上来的.南海洋壳厚度在时间上没有大幅度变化,可能是因为局部高含水量地幔的体积相对较小或体积虽大但其含水量没有显著高于地幔背景含水量.

关键词：上地幔含水量洋壳厚度海底扩张南海数值模拟海洋地质

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Effects of a Potential Autochthonous Probiotic Bacillus subtilis 2.1 on the Growth and Intestinal Microbiota of Juvenile sea Cucumber,Apostichopus japonicus Selenka

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Journal of Ocean University of china 2018年第2期17卷 363-370页

作者： ZHAO Yancui YUAN Lei WAN Junli SUN Hushan wang Yiyan ZHANG Qin School of Life Science Ludong University department of Food Engineering Shandong Business Institute Key Laboratory of marine Biotechnology Guangxi Institute of Oceanology

The effects of Bacillus subtilis 2.1 from the intestine of healthy sea cucumber on the growth, digestive enzyme activities and intestinal microbiota of juvenile sea cucumber(Apostichopus japonicus) were determined in the present study. sea cucumber was fed with Sargassum thunbergii powder supplemented with B. subtilis 2.1 at different concentrations varying among 0(control), 10~5, 10~7, and 10~9 CFU g^(-1) for 8 weeks. Results showed that the growth performance and intestinal amylase and trypsin activities were significantly increased by dietary B. subtilis 2.1 at 10~9 CFU g^(-1)(P 2.1 had no significant influence on the lipase activity in sea cucumber(P > 0.05). The polymerase chain reaction denaturing gradient gel electrophoresis and 16S rRNA gene sequencing analysis indicated that dietary B. subtilis 2.1 at 105 and 107 CFU g^(-1) inhibited most of the Proteobacteria including those in genus Vibrio. Dietary B. subtilis 2.1 at 10~9 CFU g^(-1) not only decreased the abundance and species of genus Vibrio, but also increased the intensity of genera Psychrobacter and Bacillus. A specific dosage of dietary B. subtilis 2.1 could increase the growth and modulate the intestinal microbiota of sea cucumber; thus it might be a novel probiotic for keeping the health of sea cucumber.

关键词： Apostichopus japonicus probiotic Bacillus growth digestive enzyme intestinal microbiota

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A three-dimensional coupled physical-biological model study in the spring of 1993 in the Bohai sea of china

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Acta Oceanologica Sinica 2007年第6期26卷 1-12页

作者： wang Hui LIU Guimei SUN Song HAN Boping FU Xiang Chinese Academy of Meteorological Sciences China Meteorological Administration Beijing 100081 China Key Laboratory of marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

A three-dimensional （3-D） coupled physical and biological model was used to investigate the physical processes and their influence on the ecosystem dynamics of the Bohai sea of china. The physical processes include M2 tide, time - varying wind forcing and river discharge. Wind records from 1 to 31 May in 1993 were selected to force the model. The biological model is based on a simple, nitrate and phosphate limited, lower trophic food web system. The simulated results showed that variation of residual currents forced by M2 tide, fiver discharge and time-varying wind had great impact on the distribution of phytoplankton biomass in the Laizhou Bay. High phytoplankton biomass appeared in the upwelling region. Numerical experiments based on the barotropic model and baroclinic model with no wind and water discharge were also conducted. Differences in the results by the baroclinic model and the barotropic model were significant： more patches appeared in the baroclinic model comparing with the barotropic model. And in the baroclinic model, the subsurface maximum phytoplankton biomass patches formed in the stratified water.

关键词： coupled physical-biological model ecosystem dynamics Bohai sea of china

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Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community

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Acta Oceanologica Sinica 2020年第4期39卷 110-119页

作者： Huan wang Zhangxi Hu Zhaoyang Chai Yunyan Deng Zifeng Zhan Ying Zhong Tang CAS Key Laboratory of marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China department of marine Organism Taxonomy and Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Most of reported harmful algal blooms(HABs)of microalgae(75%)have been caused by *** on the negative effects of HABs have generally focused on animals,valuable organisms in particular,and environmental factors such as dissolved oxygen and nutrients,but relatively fewer on community level,particularly that using metagenomic *** this study,we reported an investigation on the effects of a HAB caused by the dinoflagellate Prorocentrum donghaiense on the species diversity and community structure of the dinoflagellate sub-community via a pyrosequencing approach for the samples taken before,during,and after the bloom season of *** in the East china *** sequenced partial 28S rRNA gene of dinoflagellates for the field samples and evaluated the species richness and diversity indices of the dinoflagellate community,as a sub-community of the total *** obtained 800185 valid sequences(categorized into 560 operational taxonomic units,OTUs)of dinoflagellates from 50 samples and found that the biodiversity of dinoflagellate community was significantly reduced during the blooming period in comparison to that in pre-and after-blooming periods,as reflected in the four diversity indices:the species richness expressed as the number of OTUs,Chao1 index,Shannon index(evenness),and Gini-Simpson *** four indices were all found to be negatively correlated to the cell density of the bloom species *** analyses also revealed that the *** cell abundance was correlated negatively with NO3--N,and NO2--N,but positively with total nitrogen(TN)and total phosphorus(TP).Principal coordinates analysis(PCoA)showed that the community structure of dinoflagellates was markedly different among the different sampling periods,while the redundancy analysis(RDA)revealed *** abundance,salinity,NO3--N,and SiO32-were the most four significant factors shaping the dinoflagellate community *** results toget

关键词： Prorocentrum donghaiense dinoflagellate community diversity pyrosequencing East china sea

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