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Hexafluoro-2.propanol as a potent cosolvent for chemical ligation of membrane proteins

science china {2. 2011年第1期54卷 110-116页

作者： shen fei,tang shan & liu lei department of chemistry,{2.,hefei 2.002.,china key Laboratory of bioorganic phosphorus chemistry and chemical biology,ministry of education {12. of chemistry,Tsinghua university.Beijing 100084,china 1. department of chemistry University of Science and Technology of China Hefei 230026 China2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education department of chemistry Tsinghua University Beijing 100084 China

The study on membrane proteins is an important challenge mainly because of their very poor solubility in various *** traditional recombinant expression strategy and the native chemical ligation method both have difficulty in generating sufficient amounts of desired proteins with high *** studies have shown that multiply fluorinated alcohols exhibit good ability to dissolve difficult peptide sequences,especially hexafluoro-2.propanol (HFIP).In the present study we systematically studied the capability of solvents containing different percentage of HFIP in dissolving transmembrane *** both HPLC and UV analyses we concluded that 60% HFIP/8 M urea constituted a good solvent *** this solvent system we also optimized conditions to perform native chemical ligation (NCL).Under the optimized conditions we successfully achieved NCL’s for both dipeptide formation and the synthesis of a model protein (Trifolitoxin).These results suggested that HFIP was a potential cosolvent that could be used in the ligation of poorly soluble peptides for the generation of membrane proteins.

关键词： membrane protein native chemical ligation alternative solvent hexafluoro-2.propanol

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A mirror-image protein-based information barcoding and storage technology

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science Bulletin 2021年第15期66卷 1542-1549,M0004页

作者： Ji-shen Zheng Jun Liang Wei-Wei Shi Ying Li Hong-Gang Hu Chang-Lin Tian lei {2. Tsinghua-Peking Joint Center for Life sciences Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084China hefei National Laboratory for Physical sciences at the Microscale School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China Institute of Translational Medicine Shanghai UniversityShanghai 200444China

A mirror-image protein-based information barcoding and storage technology wherein D-amino acids are used to encode information into mirror-image proteins that are chemically synthesized is *** mirror-image proteins were then fused into various materials from which information-encoded objects were ***,the mirror-image proteins were extracted from the objects using biotin-streptavidin resin-mediated specific enrichment and cleaved using an Ni(Ⅱ)-mediated selective peptide *** sequencing was accomplished using liquid chromatography/tandem mass spectrometry(LC-MS/MS)and then transcoded into the recorded *** demonstrated the use of this technology to encode Chinese words into mirror-image proteins,which were then fused onto a poly(ethylene terephthalate)(PET)film and retrieved and decoded by LC-MS/MS *** to information barcoding and storage technologies using natural biopolymers,the mirrorimage biopolymers used in our technology may be more stable and durable.

关键词： Mirror-image proteins Information barcoding and storage chemical protein synthesis Protein-of-things Protein sequencing

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