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Over-expressed Genes Detected by Suppression Subtractive Hybridization in Carcinoma Derived From Transformed 16HBE Cells Induced by BPDE

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Biomedical and Environmental Sciences 2005年第5期18卷 302-306页

作者： she-juan an JIA-KUN CHEN LI-LI LIU YAN-FENG ZHAO XUE-MIN CHEN Department of Occupational and Environmental Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Occupational and Environmental Health School of Public Health Institute for Chemical Carcinogenesis Guangzhou Medical College Guangzhou 510182 Guangdong China

To screen the over differentially expressed genes in carcinoma induced by BPDE-transformed 16HBE cells （16HBE-C cells）. Methods The suppression subtractive hybridization （SSH） method was performed to profile differentially expressed genes between 16HBE-C cells and 16HBE cells. The cDNA fragments of differentially expressed genes were inserted into TA cloning vector and transformed competent E. coli strain. Positive clones were randomly picked up and identified by the colony PCR method. Dot blot was used to test the same source with the tester. The differentially expressed cDNA fragments were sequenced and compared with known genes and EST database in Genbank. Results Eight known genes were over-expressed in 16HBE-C cells including eukaryotic translation elongation factor 1 alpha 1, HIF-1 responsive RTP801, ribosomal protein L10 （RPL10）, ribosomal protein S29 （RPS29）, mitochondrion related genes, and laminin receptor 1. Three differentially expressed cDNA fragments could not be matched to the known genes but to the EST database. Conclusion The SSH method can detect differentially expressed genes between 16HBE-C and 16HBE cells. BPDE-induced carcinogenesis may be related to alteration of at least eight known genes and three unknown genes. These expression data provide a clue to further cloning novel genes and studying functions in BPDE-induced carcinoma.

关键词： Anti-BPDE Hybridization Gene

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Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells

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Biomedical and Environmental Sciences 2007年第4期20卷 307-312页

作者： HUA-JIE CHEN RI-AN YU LING-FEI HE she-juan an ZHI-GANG WU KE-DI YANG XUE-MIN CHEN Department of Occupational and Environmental Health MOE Key Laboratory of Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Occupational and Environmental Health MOE Key Laboratory of Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Occupational and Environmental Health School of Public HealthGuangdong Pharmaceutical UniversityGuangzhou510320 Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China Department of Dental Medicine The First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou 510080 Guangdong China

To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells. Methods Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses （0.625, 1.25, 2.50, 5.00 pmol/L） for 24 hours. Results Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of madl mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group. Conclusion Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing madl mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.

关键词： Selenium Cadmium Telomerase Human telomerase reverse transcriptase

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Single nucleotide polymorphisms of MAGE-A3 gene and its clinical implications in Chinese patients with non-small cell lung cancer(NSCLC)

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Chinese Journal of Cancer Research 2015年第3期27卷 301-308页

作者： Xue-Ning Yang Ling Huang Yu Chen she-juan an Xu-Chao Zhang Ri-Qiang Liao Jian Su Yi-Long Wu Guangdong Lung Cancer Institute Guangdong Provincial Key Laboratory of Translational Medicine in Lung CancerGuangdong General Hospital& Guangdong Academy of Medical Sciences

Background： Available study revealed advanced tumors have a higher expression rate of MAGE-A3 gene which has a lot of single nucleotide polymorphism（SNP） loci with polymorphisms. This study aimed to analyze the allele frequency of SNP loci in MAGE-A3 gene and investigate the relationship between MAGE-A3 gene polymorphisms and clinical ***： Tumor samples of a cohort of 191 NSCLC patients were collected. EGFR m RNA expression were detected by q RT-PCR. SNPs in whole length of MAGE-A3 gene were detected by direct sequencing. Frequencies of the SNPs were correlated to gene expression, mutation status of EGFR and clinical ***： Sequencing analysis confirmed that allele frequencies of genotypes on SNP loci rs5970360, rs5925210, rs5970361, rs5925211 and rs35123853 were CC（0.681）/CT（0.319）, CC（0.660）/CG（0.340）, CC（0.681）/CA（0.319）, AA（0.984）/AT（0.016） and GG（1.000）/GA（0.000）, respectively, which were different from the frequencies and genotypes of MAGE-A3 in SNP database. Chi-square tests showed the EGFR mR NA expression level had significant correlation with the genotypes of SNP loci rs5970360 and rs5925210. But all frequencies of each MAGE-A3 SNPs were not found significantly different between EGFR mutant and wild type patients. MAGE-A3 gene polymorphisms had no significant effects on survival of NSCLC ***： Chinese patients with NSCLC had different SNP patterns of MAGE-A3 in comparison with those in international SNP database. These MAGE-A3 SNP loci might have not prognostic significance. MAGE-A3 SNP loci rs5970360 and rs5925210 might be predictive for EGFR m RNA expression levels and helpful to the selection of patients for epidermal growth factor receptor（EGFR） targeted immunotherapy.

关键词： MAGE-A3 epidermal growth factor receptor (EGFR) non-small cell lung cancer (NSCLC) single nucleotide polymorphism (SNP)

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Detecting the spectrum of multigene mutations in non-small cell lung cancer by Snapshot assay

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Chinese Journal of Cancer 2014年第7期33卷 346-350页

作者： Jian Su Xu-Chao Zhang she-juan an Wen-Zhao Zhong Ying Huang Shi-Liang Chen Hong-Hong Yan Zhi-Hong Chen Wei-Bang Guo Xiao-Sui Huang Yi-Long Wu Medical Research Center of Guangdong General Hospital Guangdong Lung Cancer Institute Guangdong Academy of Medical Sciences

As molecular targets continue to be identified and more targeted inhibitors are developed for personalized treatment of non-small cell lung cancer(NSCLC), multigene mutation determination will be needed for routine oncology practice and for clinical trials. In this study, we evaluated the sensitivity and specificity of multigene mutation testing by using the Snapshot assay in NSCLC. We retrospectively reviewed a cohort of 110 consecutive NSCLC specimens for which epidermal growth factor receptor(EGFR) mutation testing was performed between November 2011 and December 2011 using Sanger sequencing. Using the Snapshot assay, mutation statuses were detected for EGFR, Kirsten rate sarcoma viral oncogene homolog(KRAS), phosphoinositide-3-kinase catalytic alpha polypeptide(PIK3CA), v-Raf murine sarcoma viral oncogene homolog B1(BRAF), v-ras neuroblastoma viral oncogene homolog(NRAS), dual specificity mitogen activated protein kinase kinase 1(MEK1), phosphatase and tensin homolog(PTEN), and human epidermal growth factor receptor 2(HER2) in patient specimens and cell line DNA. Snapshot data were compared to Sanger sequencing data. Of the 110 samples, 51(46.4%) harbored at least one mutation. The mutation frequency in adenocarcinoma specimens was 55.6%, and the frequencies of EGFR, KRAS, PIK3 CA, PTEN, and MEK1 mutations were 35.5%, 9.1%, 3.6%, 0.9%, and 0.9%, respectively. No mutation was found in the HER2, NRAS, or BRAF genes. Three of the 51 mutant samples harbored double mutations: two PIK3 CA mutations coexisted with KRAS or EGFR mutations, and another KRAS mutation coexisted with a PTEN mutation. Among the 110 samples, 47 were surgical specimens, 60 were biopsy specimens, and 3 were cytological specimens; the corresponding mutation frequencies were 51.1%, 41.7%, and 66.7%, respectively(P = 0.532). Compared to Sanger sequencing, Snapshot specificity was 98.4% and sensitivity was 100%(positive predictive value, 97.9%; negative predictive value, 100%). The Snapshot assay is

关键词：基因突变检测非小细胞肺癌有丝分裂原活化蛋白激酶快照表皮生长因子受体 EGFR 频谱同源基因

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永磁同步电机电流环频率响应改进策略研究

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电机与控制学报 2018年第6期22卷 107-113页

作者：肖海峰贺昱耀乔社娟西安航空学院电子工程学院陕西西安710000 西北工业大学航海学院陕西西安710072

针对永磁同步电机矢量控制电流环内存在的固有延迟环节,如电流采样、占空比计算、逆变器死区效应及数字控制延时等。在同步旋转坐标系下引入电流解耦项jωrL,分析制约电流环频率响应能力的主要迟滞因素,并对比不同电流采样时刻与脉宽调... 详细信息

针对永磁同步电机矢量控制电流环内存在的固有延迟环节,如电流采样、占空比计算、逆变器死区效应及数字控制延时等。在同步旋转坐标系下引入电流解耦项jωrL,分析制约电流环频率响应能力的主要迟滞因素,并对比不同电流采样时刻与脉宽调制(pulse width modulation,PWM)占空比更新时序对电流环频率响应的影响。提出了一种新的电流采样时机和更新输出PWM信号模式,在半个载波周期内优化采样、计算和输出时序,减小了电流环固有延时等待时间。在载波频率不变的前提下,提高电流环动态加速过程中电流的跟踪性能。仿真和实验结果与理论分析基本吻合,电流环的频率频带带宽提高了近一倍,表明该策略的有效性和正确性。

关键词：永磁同步电机频率响应占空比更新脉宽调制电流环

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芬吗通在宫腔粘连中促内膜生长的效果研究

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中国社区医师 2020年第26期36卷 58-59页

作者：佘娟常德市妇幼保健院妇科湖南常德415000

目的:分析在宫腔粘连治疗过程中应用芬吗通促内膜生长的效果。方法:2017年1月-2019年12月收治行宫腔分离术的宫腔粘连患者140例,按药物治疗方式不同分为两组,各70例。研究组应用芬吗通治疗;对照组应用补佳乐治疗。比较两组治疗效果。结... 详细信息

目的:分析在宫腔粘连治疗过程中应用芬吗通促内膜生长的效果。方法:2017年1月-2019年12月收治行宫腔分离术的宫腔粘连患者140例,按药物治疗方式不同分为两组,各70例。研究组应用芬吗通治疗;对照组应用补佳乐治疗。比较两组治疗效果。结果:研究组治疗后月经量、月经时间、月经改善率、子宫内膜厚度、再次粘连发生率及呈A型子宫内膜状态率均优于对照组,差异有统计学意义(P<0.05)。结论:在宫腔粘连患者接受手术治疗后应用芬吗通,能够有效改善子宫内膜修复情况,优化月经及子宫内膜状态,促内膜生长的效果显著。

关键词：芬吗通宫腔粘连促内膜生长

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宫安康预防稽留流产术后宫腔粘连的效果研究

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中国社区医师 2020年第25期36卷 61-62页

作者：佘娟常德市妇幼保健院妇科湖南常德415000

目的:研究宫安康预防稽留流产术后患者宫腔粘连的效果。方法:2016年11月-2019年11月选择本院收治的稽留流产患者160例为研究对象,均接受清宫手术治疗;随机分为两组,各80例。对照组接受常规抗生素治疗;观察组在抗生素基础上应用宫安康治... 详细信息

目的:研究宫安康预防稽留流产术后患者宫腔粘连的效果。方法:2016年11月-2019年11月选择本院收治的稽留流产患者160例为研究对象,均接受清宫手术治疗;随机分为两组,各80例。对照组接受常规抗生素治疗;观察组在抗生素基础上应用宫安康治疗。比较两组治疗效果。结果:观察组治疗后阴道出血时间、月经复潮时间、腹痛时间及阴道出血量均低于对照组,差异有统计学意义(P<0.05)。观察组治疗后子宫内膜厚度优于对照组,差异有统计学意义(P<0.05)。观察组宫腔粘连发生率及粘连程度均低于对照组,并发症发生率也低于对照组,差异有统计学意义(P<0.05)。结论:稽留流产患者接受清宫手术治疗后应用宫安康治疗,能够有效减少宫腔粘连发生率,改善月经情况,同时也能够显著减少术后并发症发生率。

关键词：稽留流产宫安康清宫手术治疗宫腔粘连

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缺血性脑损伤时MMPs、PA/PAI、TB对血脑屏障的影响

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神经解剖学杂志 2009年第3期25卷 353-356页

作者：吴艳云余涓翁绳美福建医科大学生理学与病理生理学系福州350004 福建医科大学药理学系福州350004

缺血性脑损伤无一例外可导致血脑屏障(blood brain barrier,BBB)结构的完整性破坏并使其通透性增加,造成严重的神经功能损伤,国内外学者为此做了大量研究.

关键词：缺血性脑损伤血脑屏障 MMPs PAI barrier 基质金属蛋白酶 TB 细胞活化

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摇摆对传热影响的机理分析

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核动力工程 2010年第3期31卷 50-54页

作者：黄振高璞珍谭思超佘莹娟哈尔滨工程大学核科学与技术学院哈尔滨150001

通过对摇摆情况下竖直加热管内流体流动的理论分析,建立了模型,推导出通道内的流速分布。研究了通道壁面附近流体的流动情况。分析认为摇摆情况下的竖直加热管内流动不同于普通的非定常流动,在通道壁面附近的边界层遭到破坏。在分析流... 详细信息

通过对摇摆情况下竖直加热管内流体流动的理论分析,建立了模型,推导出通道内的流速分布。研究了通道壁面附近流体的流动情况。分析认为摇摆情况下的竖直加热管内流动不同于普通的非定常流动,在通道壁面附近的边界层遭到破坏。在分析流动的基础上指出了竖直加热管内边界层被破坏的原因,进而指出摇摆影响传热的机理是摇摆使加热管壁面附近流动局部扰动加剧。

关键词：摇摆传热流量波动

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西汉高速公路工程水土保持效果评估分析

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西北林学院学报 2011年第6期26卷 213-218页

作者：张捐社高照良西北农林科技大学思政部陕西杨陵712100 西北农林科技大学水土保持研究所陕西杨陵712100

按照《中华人民共和国水土保持法》及配套法律、法规、规定以及国家对水土保持、环境保护的总体要求,查阅工程监理报告、监测报告、现场抽样调查以及与监测部门、当地水利部门座谈等途径,对该工程水土保持效果进行了评估。结果表明:在... 详细信息

按照《中华人民共和国水土保持法》及配套法律、法规、规定以及国家对水土保持、环境保护的总体要求,查阅工程监理报告、监测报告、现场抽样调查以及与监测部门、当地水利部门座谈等途径,对该工程水土保持效果进行了评估。结果表明:在水土流失治理方面,治理面积1 547.9hm2,项目区平均扰动土地治理率为99.60%,完成水土流失治理面积594.0hm2,平均水土流失总治理度为98.90%,土壤流失控制比为1.09～1.28,拦渣率为99.68%。生态环境和土地生产力恢复方面,植被恢复系数达到95.90%,成活率和保存率均达95%以上,植被盖度明显增加,公路建设中实现了物种多样性,植被的自然恢复达到预期的目标。公众满意程度好。

关键词：西汉高速公路工程水土保持生态工程效果评估

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