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中国有色金属学会会刊：英文版 2004年第z1期14卷 301-304页

作者： JIANG shao-qun MA xin-xin SUN Yue School of Materials Science and Engineering Harbin Institute of Technology Harbin 150001 China School of Materials Science and Engineering Harbin Institute of Technology Harbin 150001 China School of Materials Science and Engineering Harbin Institute of Technology Harbin 150001 China

AISI302 stainless steel samples were modified by elevated temperature nitrogen plasma immersion ion implantation at temperature ranging from 330 ℃ to 450 ℃. The tribological behaviors of the implanted layers of the samples were investigated. The samples were characterized by Auger electron spectroscopy (AES), glancing angle X-ray diffraction (GXRD), and nanoindentation. The results show that the implantation temperature plays an important rule on the microstructure and surface properties of the implanted layers. The thickness of the modified layer implanted at 390 ℃ is about 9 μm. It is improved about two orders compared with that of the implanted at room temperature. The surface nanohardness and the wear resistance of elevated temperature implanted layers increase significantly, and the friction coefficient decreases obviously in comparison with the unimplanted one. These data suggests that the improvement results from the formation of new phases such as ε-(Fe, Cr, Ni)2+xN, or noncrystal phase.

关键词： austenite stainless steel elevated temperature plasma immersion ion implantation tribology nanohardness

Microwave-assistant Syntheses, Crystal Structures and Safener Activities of Two Substituted Phenyl Isoxazole Derivatives

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Chinese Journal of Structural Chemistry 2021年第9期40卷 1231-1237,1111页

作者： GAO Ying-Chao shao xin-xin FU Ying College of Arts and Sciences Northeast Agricultural UniversityHarbin 150030China

Two novel substituted phenyl isoxazole benzoxazine formamide derivatives were designed and synthesized with substituted o-aminophenol,1,2-dibromoethane and different phenyl substituted isoxazole formyl chloride as the raw materials via microwave assistant *** target compounds were characterized by IR,^(1)H NMR,^(13)C NMR and *** single-crystal structures were further determined by X-ray diffraction.3-(2’-Chloro-6’-fluoro-phenyl)-4-(2’,3’-dihydro-1’,4’-benzoxazine)-5-methyl-isoxazole formamide (4a) crystallizes in orthorhombic system,P2_(1) space group with a=8.9414(18),b=10.834(2),c=17.706(4)A,V=1715.1(6)A^(3),D_(c)=1.444 Mg/m^(3),Z=4,F(000)=768,μ(Mo Kα)=0.255 mm^(-1),R=0.0406 and wR=0.1171.3-Phenyl-4-(6-methyl-2’,3’-dihydro-1’,4’-benzoxazine)-5-methyl-isoxazole formamide (4b) is of triclinic system,space group PI with a=7.7659(16),b=8.3626(17),c=13.484(3)A,α=76.04(3)°,β=100.63(3)°,γ=82.01(3)°,V=841.6(3)A^(3),D_(c)=1.319 Mg/m^(3),Z=2,F(000)=352,μ(Mo Kα)=0.090 mm^(-1),R=0.0672 and wR=*** crystals are packed through C–H···O hydrogen bonding *** is C–H···F hydrogen bond between 4a molecules,and C–H···N between 4b *** results showed that compounds 4a and 4b exhibited detoxification on maize and restored maize growth index.

关键词： isoxazole benzoxazine formamide microwave-assistant synthesis single-crystal structure safener activity

Biological Evaluation of Lysionotin:a Novel Inhibitor of 5-Lipoxygenase for Anti-glioma

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Chinese Journal of Integrative Medicine 2024年第9期30卷 826-834页

作者： shao xin-xin CHEN Cong LIU Jie LI Qing-jun HE Shan QI Xiang-hua FU Xian-jun WANG Zhen-guo The First Clinical College of Medicine Shandong University of Traditional Chinese MedicineJinan250355China Institute for Literature and Culture of Chinese Medicine Shandong University of Traditional Chinese MedicineJinan250355China Key Laboratory of Classical Theory of Traditional Chinese Medicine Ministry of EducationShandong University of Traditional Chinese MedicineJinan250355China College of Pharmacy Shandong University of Traditional Chinese MedicineJinan250355China Experimental Center Shandong University of Traditional Chinese MedicineJinan250355China Neurology Department Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan250014China Marine Traditional Chinese Medicine Research Center Qingdao Academy of Traditional Chinese MedicineShandong University of Traditional Chinese MedicineQingdao266114Shandong ProvinceChina

Objective:To explore the potential mechanism of lysionotin in treating ***:First,target prediction based on Bernoulli Naïve Bayes profiling and pathway enrichment was used to predict the biological activity of *** binding between 5-lipoxygenase(5-LO)and lysionotin was detected by surface plasmon resonance(SPR)and molecular docking,and the inhibitory effects of lysionotin on 5-LO and proliferation of glioma were determined using enzyme inhibition assay in vitro and cell viability analysis,***,the pharmaceutical effect of lysionotin was explored by cell survival rate analysis and liquid chromatography with tandem mass spectrometry(LC-MS/MS).The protein expression,intracellular calcium ion concentration and cytoskeleton detection were revealed by Western blot,flow cytometry and fluorescence labeling,***:Target prediction and pathway enrichment revealed that lysionotin inhibited 5-LO,a key enzyme involved in the arachidonic acid metabolism pathway,to inhibit the proliferation of *** docking results demonstrated that 5-LO can be binding to lysionotin through hydrogen bonds,forming bonds with His600,Gln557,Asn554,and *** analysis further confirmed the interaction between 5-LO and ***,enzyme inhibition assay in vitro and cell survival rate analysis revealed that 50%inhibition concentration of lysionotin and the median effective concentration of lysionotin were 90 and 16.58µmol/L,respectively,and the results of LC-MS/MS showed that lysionotin inhibited the production of 5S-hydroperoxy-eicosatetraenoic acid(P<0.05),and moreover,the LC-MS/MS results indicated that lysionotin can enter glioma cells well(P<0.01)and inhibit their *** blot analysis demonstrated that lysionotin can inhibit the expression of 5-LO(P<0.05)and downstream leukotriene B4 receptor(P<0.01).In addition,the results showed that lysionotin affected intracellular calcium ion concentration by inh

关键词： 5-lipoxygenase inhibitor lysionotin 5-lipoxygenase leukotriene B4 calcium ion cytoskeleton

Serum-free media for articular chondrocytes in vitro expansion

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Chinese Medical Journal 2013年第13期126卷 2523-2529页

作者： shao xin-xin Neil A. Duncan LIN Lin FU xin ZHANG Ji-ying YU Chang-long McCaig Institute for Bone and Joint Health University of Calgary Calgary Alberta Canada Schulich School of Engineering University of Calgary Calgary Alberta Canada institute of Sports Medicine the Third Hospital Peking University Beijing 100083 China

Background In vitro chondrocyte expansion is a major challenge in cell-based therapy for human articular cartilage repair. Classical culture conditions usually use animal serum as a medium supplement, which raises a number of undesirable questions. In the present study, two kinds of defined, serum-free media were developed to expand chondrocytes in monolayer culture for the purpose of cartilage tissue engineering. Methods Bovine chondrocytes were expanded in serum-free media supplemented with fibroblast growth factor-2 and platelet-derived growth factor or fibroblast growth factor-2 and insulin-like growth factor. Expansion culture in a conventional 10% fetal bovine serum （FBS） medium served as control. Fibronectin coating was used to help cell adhesion in serum-free medium. Next, in vitro three-dimensional pellet culture was used to evaluate the chondrocyte capacity. Cell pellets were expanded in different media to re-express the differentiated phenotype （re-differentiation） and to form cartilaginous tissue. The pellets were assessed by glycosaminoglycans contents, collagen II, collagen I and collagen X immunohistological staining. Results Chondrocytes cultured in serum-free media showed no proliferation difference than cells grown with 10% FBS medium. In addition, chondrocytes expanded in both serum-free media expressed more differentiated phenotypes at the end of monolayer culture, as indicated by higher gene expression ratios of collagen type II to collagen type I. Pellets derived from chondrocytes cultured in both serum-free media displayed comparable chondrogenic capacities to pellets from cells expanded in 10% FBS medium. Conclusion These findings provide alternative culture approaches for chondrocytes in vitro expansion, which may benefit the clinical use of autologous chondrocytes implantation.

关键词： articular chondrocyte serum-free media in vitro

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脉冲电源储能电容反向充电电压释放方法

电机与控制学报 2017年第8期21卷 41-47页

作者：刘建宝邵英秦昕昕海军工程大学电气工程学院湖北武汉430033

在放电主开关放置于储能电容支路的脉冲功率电源放电过程中,储能电容存在反向充电现象,反向电压无法释放,影响储能电容的使用寿命,并降低了脉冲功率电源的使用效率。在详细推导电容储能型脉冲功率电源放电过程的基础上,分析影响储能电... 详细信息

在放电主开关放置于储能电容支路的脉冲功率电源放电过程中,储能电容存在反向充电现象,反向电压无法释放,影响储能电容的使用寿命,并降低了脉冲功率电源的使用效率。在详细推导电容储能型脉冲功率电源放电过程的基础上,分析影响储能电容反向充电电压的因素,讨论降低储能电容反向充电电压的途径,将放电主开关放置于负载支路的电路结构,实现了储能电容反向充电电压的释放。仿真和实验结果表明:调整放电开关位置后的脉冲功率电源负载电流特性与调整前基本一致,完全满足电磁发射的需求;同时,反向电压的及时释放提高了储能电容的使用寿命,增加了电磁发射系统的效率。

关键词：脉冲功率电源储能电容反向充电脉冲成型网络续流硅堆电磁发射

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TI-FastText自动商品分类算法

计算机科学 2022年第S1期49卷 206-210页

作者：邵欣欣大连东软信息学院辽宁大连116023

为了实现根据商品标题信息进行商品自动分类的功能,提出了基于词频-逆文本频率(TF-IDF)的中文Fasttext商品分类方法。该方法首先利用FastText本身的特点,将词库表示成前缀树;然后对n元语法模型n-gram处理后的词典进行TF-IDF筛选,从而在... 详细信息

为了实现根据商品标题信息进行商品自动分类的功能,提出了基于词频-逆文本频率(TF-IDF)的中文Fasttext商品分类方法。该方法首先利用FastText本身的特点,将词库表示成前缀树;然后对n元语法模型n-gram处理后的词典进行TF-IDF筛选,从而在计算输入词序列向量均值时,偏向高群分度的词条;最后将文本内容以字符顺序进行大小为N的窗口滑动操作,使其更适用于商品标题分类。基于Anaconda平台,对基于FastText的商品分类算法进行实现和优化,经评估,最终的分类器准确率较高,能够满足电商平台对商品分类的需求。

关键词：商品分类中文短文本分类 FastText TF-IDF

基于Canopy和共享最近邻的服务推荐算法

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计算机科学 2020年第S02期47卷 479-481,503页

作者：邵欣欣大连东软信息学院辽宁大连116023

为辅助银行机构进行精准的服务推荐,提出了基于改进的Canopy和共享最近邻相似度的聚类算法。基于该算法对用户进行细分,针对用户群特点进行精准服务推荐。该算法首先采用最大值和最小值对Canopy算法进行改进,并应用该算法得出初始聚类结... 详细信息

为辅助银行机构进行精准的服务推荐,提出了基于改进的Canopy和共享最近邻相似度的聚类算法。基于该算法对用户进行细分,针对用户群特点进行精准服务推荐。该算法首先采用最大值和最小值对Canopy算法进行改进,并应用该算法得出初始聚类结果,然后利用共享最近邻相似度算法对聚类结果中的交叉部分数据进行归类,最终得出用户聚类数据。该算法在某银行对真实客户数据进行应用,选择基于客户的贡献度、忠诚度和活跃度3个指标进行聚类,结果表明,该算法提高了客户细分的质量和聚类的效率,聚类结果对于客户的消费数据刻画非常准确,能够为银行的精准服务推荐提供数据支持。

关键词：客户聚类服务推荐 Canopy算法共享最近邻相似度聚类指标

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu xin shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

靶向5-LOX中药黄酮类化合物的活性筛选及其作用方式和共性规律研究

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天然产物研究与开发 2019年第9期31卷 1506-1514页

作者：邵欣欣陈聪王振国付先军山东中医药大学中医文献与文化研究院山东中医药大学中医经典理论教育部重点实验室山东中医药大学中药网络药理学实验室济南250355

筛选靶向结合炎症相关蛋白5-LOX(5-lipoxygenase,5-脂氧合酶)的中药黄酮类天然产物,分析与5-LOX结合的黄酮类成分及其来源中药的共性规律。本研究借助Discovery Studio 2017 R2分子对接和药效团构建模块,结合SPR分子筛选实验,以及关联... 详细信息

筛选靶向结合炎症相关蛋白5-LOX(5-lipoxygenase,5-脂氧合酶)的中药黄酮类天然产物,分析与5-LOX结合的黄酮类成分及其来源中药的共性规律。本研究借助Discovery Studio 2017 R2分子对接和药效团构建模块,结合SPR分子筛选实验,以及关联网络构建的方法进行研究。研究结果显示,来源于17种中药的18个黄酮类小分子中有11个能够与5-LOX结合,并从分子对接以及药效团构建研究中发现其作用的3种方式和共性特征:(1)部分中药黄酮成分(如木犀草素等)通过结构中的B环与5-LOX在活性位点ASP243形成静电中心相结合;(2)部分中药黄酮成分(如芹菜素等)是通过结构中的A环与活性位点VAL520形成疏水键、与活性位点ASP243形成氢键与5-LOX结合;(3)杨梅苷等黄酮类成分由于极性较强,在没有形成疏水键的情况下,也是通过形成静电中心与5-LOX在活性位点ASP243产生相互作用。此外还发现靶向5-LOX的活性中药黄酮类化合物,大多来源于具有利湿、退黄等功效,性味甘苦寒的景天科中药中。本研究发现了部分靶向5-LOX的中药黄酮类成分及其作用方式和共性规律,为开发靶向5-LOX抗肿瘤新药提供思路和方法。

关键词： 5-LOX 中药黄酮类化合物先导化合物

是“陶片放逐”还是“贝壳流放”?——“Ostracism”释义

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史学集刊 2009年第2期 41-44页

作者：邵欣欣郭小凌北京师范大学历史学院北京100875

"Ostracism"是公元前5世纪古代雅典一项重要的政治制度。但是这一制度的中文译名却不尽相同,有的称之为"陶片放逐",有的称之为"贝壳流放"。通过辨析"Ostracism"的物质载体"Ostrakon"的含义,无论是从语义方面还是从考古证据来说,"陶片放... 详细信息

"Ostracism"是公元前5世纪古代雅典一项重要的政治制度。但是这一制度的中文译名却不尽相同,有的称之为"陶片放逐",有的称之为"贝壳流放"。通过辨析"Ostracism"的物质载体"Ostrakon"的含义,无论是从语义方面还是从考古证据来说,"陶片放逐"的译法均比"贝壳流放"更加合理。中文译名之所以发生分歧,是因为客观资料的限制和苏联史学译作的影响。

关键词： Ostracism 陶片放逐贝壳流放