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先驱体转化SiBCN陶瓷的制备、性能与应用

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航空制造技术 2024年第1期67卷 40-65页

作者：邵长伟王驰原龙鑫王小宙国防科技大学空天科学学院新型陶瓷纤维及其复合材料重点实验室长沙410073

先驱体转化法是制备高性能陶瓷材料的重要方法,尤其在连续纤维及其复合材料(FRCMC)的制备、元素组成与微结构调控等方面具有显著优势。先驱体转化SiBCN陶瓷具有多元素含量可调、化学键合结构可控的特点,构建了不同结构特征和特殊性能的... 详细信息

先驱体转化法是制备高性能陶瓷材料的重要方法,尤其在连续纤维及其复合材料(FRCMC)的制备、元素组成与微结构调控等方面具有显著优势。先驱体转化SiBCN陶瓷具有多元素含量可调、化学键合结构可控的特点,构建了不同结构特征和特殊性能的陶瓷材料。近几年,先驱体转化SiBCN陶瓷发展呈现出新的特点,结构功能一体化设计与制备技术受到了国内外的广泛关注。本文主要梳理了2016年至今先驱体转化SiBCN陶瓷的国内外研究进展,首先简要介绍先驱体转化SiBCN陶瓷的主要特点,然后以先驱体转化陶瓷产物的典型特点为分类依据,分别从SiBCN陶瓷先驱体及其陶瓷产物、连续SiBCN陶瓷纤维、SiBCN基复合材料和功能化SiBCN陶瓷4个方面综述了主要研究进展,提出了未来发展趋势和重点任务,期望为SiBCN陶瓷研制与应用研究提供参考,促进我国先进陶瓷材料的发展进步。

关键词：先驱体转化陶瓷硅硼碳氮陶瓷(SiBCN) 陶瓷纤维陶瓷基复合材料功能陶瓷

High-temperature oxidation behavior of SiBN fibers in air

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Journal of Advanced Ceramics 2021年第4期10卷 768-777页

作者： Xin LONG Zhenyu WU changwei shao Xiaozhou WANG Yingde WANG Science and Technology on Ceramic Fibers and Composites Laboratory College of Aerospace Science and EngineeringNational University of Defense TechnologyChangsha 410073China

SiBN fibers are one of the most admirable microwave-transparent reinforced materials for high Mach number ***,the detailed high-temperature oxidation behavior of SiBN fibers has not been studied *** this work,we studied the high-temperature oxidation behavior of SiBN fibers with different boron contents at the temperature range of 1000-1400℃in *** fibers started to be oxidized at 1100℃,with Si_(3)N_(4) and BN phase oxidized to SiO2 and B_(2)O_(3),*** to the gasification and the escape of molten B_(2)O_(3) at high temperatures,amorphous SiO_(2) could be remained at the fiber *** the fiber further oxidized,the molten B_(2)O_(3) at the inside may infiltrate into the fiber interior to react with Si_(3)N_(4),causing the precipitation of hexagonal boron nitride(h-BN)nanoparticles and the formation of SiO_(2)/BN ***,complex oxidation layers with two distinct concentric sublayers accompanied with two transition sublayers could be formed after the oxidizing treatment.

关键词： microwave-transparent SiBN fibers high-temperature oxidation resistance

Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts

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Acta Oceanologica Sinica 2013年第8期32卷 35-43页

作者： shao changwei LIU Geng LIU Shanshan LIU Changlin CHEN Songlin Ocean University of China Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences BGI-Shenzhen

The cyp19ala gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome （BAC） sequence of half-smooth tongue sole （Cynoglossus sernilaeuis） containing the intact cyp19ala gene was reported and the conservation and synteny of the cyp19ala gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine （GC） content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19ala gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The ex- pression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19ala genes not only have similar genomic structures, but also have conserved function- s. The genomic environment of cyp19ala in tongue sole is not universal in teleosts, reflecting the particular evolution of tongue sole cyp19ala after it diverged from the other teleosts.

关键词： BAC cypl9ala conservation gene synteny half-smooth tongue sole

Molecular Characterization and Expression and DNA Methylation Analyses of a Galectin-Related Protein Gene from Cynoglossus semilaevis

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Journal of Ocean University of China 2018年第5期17卷 1189-1196页

作者： XIU Yunji GAN Tian XU Wenteng shao changwei CHEN Songlin Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Marine Science and Engineering College Qingdao Agricultural University College of Fisheries and Life Science Shanghai Ocean University

Galectins, a family of β-galactoside-binding proteins, participate in both innate immunity and adaptive immunity. This study identified one novel galectin-related protein from half-smooth tongue sole Cynoglossus semilaevis, which was designated as CsGRP. The full-length cDNA of its encoding gene was 785 bp in length with a 528 bp open reading frame encoding a putative protein of 176 amino acids. The deduced CsGRP contained a putative 131-aa galactoside-binding lectin domain and 3 critical residues responsible for carbohydrate binding （R93, W109 and R114）. Genomic structural analysis revealed that CsGRP consisted of five exons and four intzons. CsGRP showed 68% similarity with Poecilia latipinna GRP and 67% similarity wih Stegastes partitus GRP. CsGRP showed the highest expression level in liver, although its expression was detected in all tested tissues. When challenged with Vibrio harveyi, the expression of CsGRP was significantly down-regulated in liver （P〈 0.05）. In addition, we found that in spleen and kidney of C. semilaevis, the CpG island of CsGRP showed significantly higher （P 〈 0.05） methylation level in disease-resistant family of C. semilaevis （DR-Cs） than in disease-susceptible ones （DS-Cs）. Our results suggested that CsGRP may play important roles in the immune response of C. semilaevis. Moreover, DNA methylation patterns provided valuable data for understanding the relationship between epigenetic regulation and immunity, which would assist the animal genetic research and improve the animal breeding in the future.

关键词： Cynoglossus semilaevis galectin-related protein expression pattern DNA methylation immune response

Fabrication and oxidation resistance of silicon nitride fiber reinforced silica matrix wave-transparent composites

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Journal of Materials Science & Technology 2019年第12期35卷 2761-2766页

作者： Xuejin Yang Bin Li Duan Li changwei shao Changrui Zhang Chunrong Zou Kun Liu Science and Technology on Advanced Ceramic Fibers and Composites Laboratory National University of Defense TechnologyChangsha 410073China School of Materials Sun Yat-sen UniversityGuangzhou 510275China Advance Interdisciplinary Technology Research Center Academy of Military ScienceBeijing 100010China Beijing Institute of Special Electromechanical Technology Beijing 100012China

Wave-transparent ceramic matrix composites for the high temperature use should possess excellent oxidation resistance. In this work, Si3N4f/SiO2 composites with different fiber content were fabricated by filament winding and sol gel method. The oxidation resistance was investigated by tracking the response of flexural strength to the testing temperature. The results show that the flexural strength and toughness of the composites with fiber content of over 37% can reach high levels at around 175.0 MPa and 6.2 MPa m^1/2, respectively. After 1 h oxidation at 1100℃, the flexural strength drops a lot but can still reach 114.4 MPa, which is high enough to ensure the safety of structures. However, when the oxidation temperature rises to 1200–1400℃, the flexural strengths continue to fall to a relatively low level at 50.0–66.4 MPa. The degradation at high temperatures is caused by the combination of over strong interfacial bonding, the damage of fiber and the crystallization of silica matrix.

关键词： Si3N4f/SiO2 Fiber content Oxidation resistance Mechanical property

Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole(Cynoglossus semilaevis)

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Acta Oceanologica Sinica 2016年第6期35卷 19-28页

作者： DONG Zhongdian QI Qian ZHANG Ning shao changwei ZHANG Liyan WEN Haishen CHEN Songlin Fisheries College Ocean University of China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Function Laboratory for Marine Fisheries Science and Food Production Processes National Laboratory for Marine Science and Technology

The signaling molecule hedgehog（Hh） is essential for cellular signaling required for body pattern formation in embryonic and individual development in vertebrate and *** 1（Ptc1）,a receptor of Hh,mediates the activity of Hh by binging the Hh *** this study,the Ptc1 of half-smooth tongue sole Cynoglossus semilaevis（CsPtc1） was cloned and *** full-length of CsPtc1 cDNA is 5 212 nucleotides（nt）,and encodes a protein with 1 543 amino acid ***1 shared many features with Ptc1 of other telesot ***-time quantitative PCR revealed CsPtc1 mRNA expressed in brain,liver,heart,gill,intestines,spleen,gonad and kidney,with expression level in testis significantly higher than in *** testis,hybridization signals were mainly detected in primary spermatocytes,secondary spermatocytes,and sertoli cells,while weak signals were found in ***,the degree of methylation is higher in female than in male and *** indicate that CsPtc1 may be involved in Desert Hedgehog（DHH） maintenance of the male and pseudo-male germ line and spermatogenesis.

关键词： half-smooth tongue sole male and female Patched 1 RACE qRT-PCR methylation

QTL Detection for Albinism-Related Loci in Chinese Tongue Sole(Cynoglossus semilaevis)

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Journal of Ocean University of China 2018年第6期17卷 1404-1410页

作者： LIU Yang XU Wenteng ZHANG Bo shao changwei WANG Xiaoxia SUN Hejun WANG Na CHEN Songlin Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences (CAFS) Key Laboratory for Sustainable Development of Marine Fisheries Ministry of Agriculture Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Tianjin Bohai Sea Fisheries Research Institute Bohai Sea Fisheries Research Center of Chinese Academy of Fishery Sciences

The Chinese tongue sole(Cynoglossus semilaevis) is widely cultured in the coastal region of East Asia and has excellent economic value. However, the high albino rate of the breeding population has caused a significant loss to the aquaculture industry. To study the molecular mechanism of albinism, the present study used an albino Chinese tongue sole family to construct three simple sequence repeat(SSR) linkage groups, and draft a preliminary linkage map related to albinism. After albinism-related loci mapping, 18 albinism-related loci were detected under two models(containing 2407 genes) compared to the Chinese tongue sole genome. One of these loci, the tyrosinase related protein(tyrp2), which has been reported previously as an important gene regulating both eumelanin and phaeomelanin levels, was indicated to be the possible cause of albinism. Thirty-five Gene Ontology(GO) terms and 14 Kyoto Encyclopedia of Genes and Genome pathways were annotated via bioinformatic analyses. One GO term with protein tyrosine kinase activity, which contained 10 genes, was previously suggested to affect fish albinism. These results establish a foundation for further in-depth study of albinism in Chinese tongue sole.

关键词： albinism Cynoglossus semilaevis locus mapping simple sequence repeat

Construction of a microsatellite-based genetic linkage map for half-smooth tongue sole Cynoglossus semilaevis

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Current Zoology 2013年第1期59卷 99-108页

作者： Wentao SONG Guidong MIAO Yongwei ZHAO Yuze NIU Renyi PANG Xiaolin LIAO changwei shao Songlin CHEN Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China College of Fisheries and Life Science Shanghai Ocean University Shanghai 201306 China

The half-smooth tongue sole Cynoglossus semilaevis is an important cultured marine fish and a promising model fish for the study of sex determination. Sex-specific genetic linkage maps of half-smooth tongue sole were developed with 567 mark- ers （565 microsatellite markers and two SCAR markers）. The parents and F1 progeny （92 individuals） were used as segregating populations. The female map was composed of 480 markers in 21 linkage groups, covering a total of 1388.1 cM, with an average interval 3.06 cM between markers. The male map consisted of 417 markers in 21 linkage groups, spanning 1480.9 cM, with an average interval of 3.75 cM. The female and male maps had 474 and 416 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1522.9 cM for females and 1649.1cM for males. Based on estimations of map length, the female and male maps covered 91.1% and 89.8% of the genome, respectively. Furthermore, two female-specific SCAR mark- ers, f-382 and f-783, were mapped on LG15f （linkage group 15 in female maps）. The present study presents a mid-density genetic linkage map for half-smooth tongue sole. These improved genetic linkage maps may facilitate systematic genome searches to identify quantitative trait loci （QTL）, such as disease resistance, growth and sex-related traits, and are very useful for marker-assisted selection breeding programs for economically important traits in half-smooth tongue sole [Current Zoology 59 （1）： 99-108, 2013].

关键词： Half-smooth tongue sole Cynoglossus semilaevis Microsatellite Genetic linkage map MAS

Molecular cloning,characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole(Cynoglossussemilaevis)

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Acta Oceanologica Sinica 2011年第1期30卷 68-77页

作者： DONG Xiaoli CHEN Songlin JI Xiangshan shao changwei College of Fisheries Ocean University of China Qingdao 266003 China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry ofAgriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 China College of Animal Science and Technology Shandong Agricultural University Taian 271018 China

Two transcription factors, Sox9a and Foxl2 were cloned from half-smooth tongue sole （Cynoglossus semilaevis）. Sox9a is a new duplication of *** Sox9 gene. The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2 were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic ceils. Foxl2 was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.

关键词： half-smooth tongue sole Cynoglossus semilaevis Sox9 Foxl2 real-time PCR sexreversal neo-male