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Characterization of a Novel Gain-of-Function Spotted-Leaf Mutant with Enhanced Disease Resistance in Rice

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Rice science 2019年第6期26卷 372-383页

作者： CHEN Ting CHEN Zheng Atul Prakash SATHE ZHANG Zhihong LI Liangjian shang huihui TANG Shaoqing ZHANG Xiaobo WU Jianli State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement China National Rice Research Institute

We here reported the identification and characterization of a novel gain-of-function spotted-leaf 26 (Spl26) mutant from an ethylmethylsulfone (EMS)-induced rice cultivar IR64. Spl26 displayed reddish- brown lesions that firstly appeared on the leaf tips at the early tillering stage and spread gradually downward to cover the whole leaf blades that wilted subsequently. The lesion development was light- dependent under natural conditions. Spl26 exhibited impaired photosynthetic capacity with decreased chlorophyll content and lowered photosynthetic parameters which ultimately led to the poor performance of agronomic traits. Severe cell death occurred in Spl26 in accompany with increased malonaldehyde level and membrane ion leakage rate, elevated reactive oxygen species (ROS) accumulation, altered ROS scavenging activities, increased DNA fragmentation and decreased soluble protein levels. Defense responses were activated in Spl26 with enhanced resistance to rice bacterial blight, up-regulation of defense response genes and altered endogenous hormone levels. The spotted-leaf phenotype is controlled by a single dominant nuclear gene localized to a 305 kb region between RM5490 and InDel42 on the short arm of chromosome 7. The data suggested that Spl26 is a novel gain-of-function spotted-leaf mutant with enhanced bacterial disease resistance and immunity-associated premature leaf senescence and would provide the basis for cloning of the target gene.

关键词： defense response rice reactive oxygen species senescence spotted-leaf disease resistance

ORYZA SATIVA SPOTTED-LEAF 41(OsSPL41) Negatively Regulates Plant Immunity in Rice

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Rice science 2023年第5期30卷 426-436,I0017-I0020页

作者： TAN Jingyi ZHANG Xiaobo shang huihui LI Panpan WANG Zhonghao LIAO Xinwei XU Xia YANG Shihua GONG Junyi WU Jianli State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China

Identification of immunity-associated leucine-rich repeat receptor-like protein kinases(LRR-RLK) is critical to elucidate the LRR-RLK mediated mechanism of plant ***,we reported the map-based cloning of a novel rice SPOTTED-LEAF 41(Os SPL41) encoding a putative LRR-RLK protein(Os LRR-RLK41/Os SPL41) that regulated disease responses to the bacterial blight pathogen Xanthomonas oryzae ***(Xoo).An 8-bp insertion at position 865 bp in a mutant spotted-leaf 41(spl41) allele led to the formation of purple-brown lesions on *** complementation by the wild type allele(Os SPL41) can rescue the mutant phenotype,and the complementary lines showed similar performance to wild type in a number of agronomic,physiological and molecular *** SPL41 was constitutively expressed in all tissues tested,and Os SPL41 contains a typical transmembrane domain critical for its localization to the cell *** mutant exhibited an enhanced level of resistance to Xoo in companion of markedly up-regulated expression of pathogenesis-related genes such as Os PR10a,Os PAL1 and Os NPR1,while the level of salicylic acid was significantly increased in *** contrast,the over-expression lines exhibited a reduced level of H_(2)O_(2) and were much susceptible to Xoo with down-regulated expression of pathogenesis-related *** results suggested that Os SPL41 might negatively regulate plant immunity through the salicylic acid signaling pathway in rice.

关键词： bacterial blight leucine-rich repeat receptor-like protein kinase plant immunity reactive oxygen species rice spotted leaf

Mechanisms of resistance to trastuzumab in HER2-positive gastric cancer

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Chinese Journal of Cancer Research 2024年第3期36卷 306-321页

作者： Zhifei Li Huan Zhao huihui Hu Haili shang Yongjing Ren Wenhui Qiu Hao Su Huifang Lyu Xiaobing Chen Department of Oncology the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalHenan Engineering Research Center of Precision Therapy of Gastrointestinal CancerZhengzhou Key Laboratory for Precision Therapy of Gastrointestinal CancerZhengzhou 450008China Henan University of Traditional Chinese Medicine Zhengzhou 450046China State Key Laboratory of Esophageal Cancer Prevention&Treatment Key Laboratory of Advanced Drug Preparation TechnologiesMinistry of EducationSchool of Pharmaceutical SciencesZhengzhou UniversityZhengzhou 450001China The First Affiliated Hospital of Xinxiang Medical University Xinxiang 453000China Department of Oncology the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalZhengzhou 450008China

Gastric cancer is one of the most prevalent cancers worldwide,and human epidermal growth factor receptor 2(HER2)-positive cases account for approximately 20%of the total ***,trastuzumab+chemotherapy is the recommended first-line treatment for patients with HER2-positive advanced gastric cancer,and the combination has exhibited definite efficacy in HER2-targeted ***,the emergence of drug resistance during treatment considerably reduces its effectiveness;thus,it is imperative to investigate the potential mechanisms underlying *** the present review article,we comprehensively introduce multiple mechanisms underlying resistance to trastuzumab in HER2-positive gastric cancer cases,aiming to provide insights for rectifying issues associated with resistance to trastuzumab and devising subsequent treatment strategies.

关键词： HER2 gastric cancer trastuzumab drug resistance

Evolution of template-assisted two-dimensional porphyrin chiral grating structure by directed self-assembly using chiral second harmonic generation microscopy

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Chinese Chemical Letters 2024年第10期35卷 170-174页

作者： Changhui Yu Peng shang huihui Hu Yuening Zhang Xujin Qin Linyu Han Caihe Liu Xiaohan Liu Minghua Liu Yuan Guo Zhen Zhang Beijing National Laboratory for Molecular Sciences State Key Laboratory of Molecular Reaction DynamicsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Beijing National Laboratory for Molecular Science CAS Key Laboratory of ColloidInterface and Chemical ThermodynamicsInstitute of ChemistryChinese Academyof SciencesBeijing100190China

Directed self-assembly has been used to create micro-nano scale patterns,including chiral periodic structures of organic molecules,for potential applications in optics,photonics,metamaterials,and medical and sensing *** study presents a straightforward approach for fabricating large-scale chiral grating porphyrin assemblies through template-assisted *** solution of tetrakis(4-sulfonatophenyl)porphyrin(TPPS)was induced by chiral amino acids(L/D-arginine and L/D-serine)to selfassemble into highly ordered chiral grating structures with the assistance of sodium dodecyl sulfate(SDS).The structures show precise line widths(5.5μm)and gaps(18μm).Using in situ optical microscopy and second harmonic generation(SHG)microscopy,the chiral characteristics and dynamic evolution of the template-assisted self-assembly are *** is found that the chirality of amino acids induced TPPS self-assembled into chiral structures and the liquid contraction interface significantly enhanced the chirality of the *** study is significant for understanding the mechanism of chiral evolution and designing novel micro-nano materials with predetermined chiral properties.

关键词： Second harmonic generation microscopy Chiral assembly Porphyrin Chiral evolution Template-assisted self-assembly Micro-nano structure

Mg-4.4Li-2.5Zn-0.46Al-0.74Y合金高温变形流动应力、组织演变与本构分析

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金属学报 2021年第7期57卷 860-870页

作者：曹富荣丁鑫项超尚会会东北大学材料科学与工程学院沈阳110819 东北大学轻质结构材料辽宁省重点实验室沈阳110819 东北大学轧制与连轧自动化国家重点实验室沈阳110819 四川航天长征装备制造有限公司成都610000

采用多向锻造与轧制(MDFR)制备了Mg-4.4Li-2.5Zn-0.46Al-0.74Y合金,利用拉伸机、OM和XRD等研究了热拉伸流动应力、组织演变、本构模型与变形机理。结果表明,合金多向锻造晶粒细化机理为机械式剪切破碎与动态再结晶细化。合金在523~573 K... 详细信息

采用多向锻造与轧制(MDFR)制备了Mg-4.4Li-2.5Zn-0.46Al-0.74Y合金,利用拉伸机、OM和XRD等研究了热拉伸流动应力、组织演变、本构模型与变形机理。结果表明,合金多向锻造晶粒细化机理为机械式剪切破碎与动态再结晶细化。合金在523~573 K时,热变形组织主要发生动态回复与动态再结晶;≥573 K时,热变形组织主要发生晶粒长大;在623 K发生由晶粒长大引起的应变硬化。合金退火组织由α(Mg)、β(Li)、Al_(2)Y、Al_(12)Mg_(17)、LiAl和Mg_(2)Y相组成。本构分析表明,合金应力指数为4.4,变形激活能为120.40 kJ/mol;623 K、1.67×10^(-4)s^(-1)条件下,对应240%延伸率的位错密度和数量与原子扩散计算表明,合金在该条件下的变形机理为晶格扩散控制的位错蠕变。晶粒长大模型确定该条件下的晶粒长大指数q=2,比例因子α’=0.2。

关键词： Mg-Li合金热拉伸显微组织力学性能本构分析变形机理

红球菌R04细胞的不对称分裂及其在联苯胁迫下的分裂抑制

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微生物学报 2018年第5期58卷 893-906页

作者：杨秀清商慧慧山西大学生物技术研究所化学生物学与分子工程教育部重点实验室山西太原030006

【目的】研究红球菌R04细胞的分裂方式及联苯对其形态和细胞分裂的影响。【方法】以一株多氯联苯降解菌株(Rhodococcus sp.R04)为研究对象,利用荧光显微镜、扫描电子显微镜及透射电子显微镜分析红球菌R04在不同培养条件下的细胞分裂。... 详细信息

【目的】研究红球菌R04细胞的分裂方式及联苯对其形态和细胞分裂的影响。【方法】以一株多氯联苯降解菌株(Rhodococcus sp.R04)为研究对象,利用荧光显微镜、扫描电子显微镜及透射电子显微镜分析红球菌R04在不同培养条件下的细胞分裂。【结果】红球菌R04细胞表现出对称分裂(约占30%)和不对称分裂(约占70%)两种分裂方式,且培养条件不影响不对称分裂细胞所占的比例。细胞分裂过程中,隔膜主要分布于细胞长度的30%–50%。在联苯的分解代谢过程中,红球菌R04细胞的生长分裂会受到联苯的抑制,但不影响红球菌R04细胞的分裂方式,在联苯胁迫下,细胞形成丝状化,表现出异常分裂,随着培养时间的延长,在细胞生长指数后期至转换期,细胞能够进行正常分裂。【结论】环境异生型化合物联苯/多氯联苯对其降解菌株——红球菌R04细胞的生长和分裂有较强影响,但是并不影响其分裂方式。

关键词：不对称分裂细菌细胞分裂联苯/多氯联苯红球菌R04

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离网误差迭代自校正STAP算法

信号处理 2021年第7期37卷 1277-1284页

作者：尚慧慧高志奇黄平平内蒙古工业大学信息工程学院内蒙古呼和浩特010080 内蒙古自治区雷达技术与应用重点实验室内蒙古呼和浩特010051

基于稀疏恢复技术的空时自适应处理(Sparse Recovery Space-Time Adaptive Processing,SR-STAP)方法提升了动目标检测性能。然而,当出现离网效应时,SR-STAP算法的杂波抑制性能下降。为了解决离网效应问题,本文提出了一种离网误差迭代自... 详细信息

基于稀疏恢复技术的空时自适应处理(Sparse Recovery Space-Time Adaptive Processing,SR-STAP)方法提升了动目标检测性能。然而,当出现离网效应时,SR-STAP算法的杂波抑制性能下降。为了解决离网效应问题,本文提出了一种离网误差迭代自校正STAP算法。该算法首先从常规STAP字典中选取了功率谱值较高的网格点来构建全局字典。然后,从全局字典中找到与杂波点匹配度较高的原子为中心,构建局部STAP字典,并利用贝叶斯后验概率最大思想进行局部搜索,找到与杂波点匹配度最高的网格点。最后,得到了经过修正后的STAP字典和最优STAP滤波权,优化了离网情况下STAP算法性能。通过仿真验证了所提算法消除离网误差的有效性。

关键词：空时自适应技术稀疏恢复离网效应后验贝叶斯思想

Smart Breeding Platform:A web-based tool for high-throughput population genetics,phenomics,and genomic selection

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Molecular Plant 2024年第5期17卷 677-681页

作者： huihui Li Xin Li Peng Zhang Yingwei Feng Junri Mi shang Gao Lele Sheng Mohsin Ali Zikun Yang Liang Li Wei Fang Wensheng Wang Qian Qian Fei Gu Wenbin Zhou State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Nanfan Research Institute CAASSanyaHainan 572024China DAMO Academy Alibaba GroupHangzhou 310023China Hupan Lab Hangzhou 310023China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya CityHainan Province 572024China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhouZhejiang 310006China

Dear Editor,In the era of big data and artificial intelligence,"smart breeding"has become a broad conceptual framework encompassing the paradigm shift of crop breeding to relying on analysis of high-throughput population genetics and phenomics data to conduct genomic selection,allowing identification and optimal use of the genetic potential in crop species(Xiao et al.,2022;Xu et al.,2022;Wang et al.,2023).Most existing tools for analyzing high-throughput breeding data require extensive computational power,complex installation processes,and command-line expertise and are therefore challenging and inconvenient for the majority of researchers and breeders(Brandies and Hogg,2021).

关键词： breeding artificial breed

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开式机械压力机机身校核与优化模块的开发

锻压技术 2015年第2期40卷 148-153页

作者：夏琴香尚慧慧徐海涛李振石华南理工大学机械与汽车工程学院广东广州510640 广东锻压机床厂有限公司广东佛山528300

开式机械压力机机身为悬臂梁结构,强度、刚度较差且校核计算复杂。实际设计中通常采用有限元法进行运算,工作量大、耗时长,尤其是对于设计需要进行反复修改和优化计算的情况下。首先对机身的强度、刚度计算和优化的基本要素进行了分析,... 详细信息

开式机械压力机机身为悬臂梁结构,强度、刚度较差且校核计算复杂。实际设计中通常采用有限元法进行运算,工作量大、耗时长,尤其是对于设计需要进行反复修改和优化计算的情况下。首先对机身的强度、刚度计算和优化的基本要素进行了分析,其次介绍了机身校核与优化模块的开发过程,最后以实例验证了该模块的有效性。结果表明,所开发的模块能有效地实现对机身的校核和优化,可极大地提高机身设计效率、缩短产品的开发周期。

关键词：压力机机身 UG二次开发强度及刚度校核机身重量优化