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Journal of Ocean University of China 2022年第4期21卷 1027-1036页

作者： shan yanan ZHENG Jinhui GAO Hong SUN Jinsheng Tianjin Key Laboratory of Animal and Plant Resistance College of Life SciencesTianjin Normal UniversityTianjin 300387China

Toll like receptors(TLRs)are the main innate immune‘pattern recognition receptors’of animals,which play a central role in host cell recognition and responses to invasive pathogens,particularly common structures of microbial pathogens.In this study,the gene expression profiles of TLRs in the spleen,head kidney,gill,small intestine,liver,muscle,and heart of healthy Paralichthys olivaceus were detected by real-time quantitative PCR(qPCR).The TLR family members were widely expressed in different tissues with different basic expression profiles.The highest expressions of TLR1,5m,7,8,9,14,and 21 were found in the spleen;the highest expressions of TLR3 and TLR21 were found in the gill;the highest expressions of TLR2 and 5s were found in the small intestine.The second highest expressions of TLR3,7,and 8 were found in small intestine.The gene expression profiles of TLRs stimulated with Edwardsiella tarda DNA,RNA,and lipopolysaccharide(LPS)were also detected in spleen,head kidney and gill.TLR9 and TLR21 were sensitive to E.tarda DNA;TLR 8 and TLR21 were sensitive to E.tarda RNA;and TLR1 and TLR14 were sensitive to E.tarda LPS.The expressions of the other TLR genes showed no significant changes.The results imply that the expressions of these TLR genes in P.olivaceus are differently regulated in the whole body and play important roles in the immune response against E.tarda infection.

关键词： Edwardsiella tarda Paralichthys olivaceus TLRs expression real-time fluorescence quantitative PCR

Global Integrability for Solutions to Obstacle Problems

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Journal of Partial Differential Equations 2022年第4期35卷 320-330页

作者： shan yanan GAO Hongya Hebei Key Laboratory of Machine Learning and Computational Intelligence College of Mathematics and Information ScienceHebei UniversityBaoding 071002China

DenoteκψØ(Ω)={υ∈w1,p(Ω):υ≥ψ,a,e.andυ-Ø∈w1,po(Ω)},where is any function in Q C R^(N),N≥2,with values in RU[±∞]and e is a measurable function.This paper deals with global integrability for u E Kμ,e such ... 详细信息

关键词： Global integrability obstacle problem A-harmonic equation

Regularity for minimizing sequences of some variational integrals

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Science China Mathematics 2023年第4期66卷 777-798页

作者： Hongya Gao yanan shan Wei Ren Hebei Key Laboratory of Machine Learning and Computational Intelligence College of Mathematics and Information ScienceHebei UniversityBaoding 071002China

This paper deals with regularity properties for minimizing sequences of some integral functionals related to the nonlinear elasticity theory.Under some structural conditions,we derive that the minimizing sequence and ... 详细信息

关键词： regularity minimizing sequence variational integral energy Ekeland variational principle

Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway

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Cancer Biology & Medicine 2018年第4期15卷 400-414页

作者： Lianmei Zhao Jie Li Yueping Liu Wei Zhou yanan shan Xinyi Fan Xinliang Zhou Baoen shan Yongmei Song Qimin Zhan State Key Laboratory of Molecular Oncology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences&Peking Union Medical College Research Center The Fourth Hospital of Hebei Medical University Pathology Department The Fourth Hospital of Hebei Medical University Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Laboratory of Molecular Oncology Peking University Cancer Hospital &Institute

Objective:Small cell lung carcinoma(SCLC)is considered one of the most aggressive types of lung cancer due to its rapid growth and early metastasis.No tumor markers or therapeutic targets have been demonstrated to be specific or effective in SCLC to date.This study aims to evaluate the potential of Flotillin1(Flot1)as a target of SCLC treatment.Methods:Flot1 expression level in the tissue of SCLC and other tissue of lung disease was detected using immunohistochemical staining.Transwell and Matrigel assays were employed to examine migration and invasion of cancer cells.Flow cytometry and xCELLigence system were used to evaluate cell apoptosis and cell viability,respectively.Expression levels of Flot1,epithelialmesenchymal transition(EMT)marker E-cadherin,vimentin,cyclinD1,TGF-β-Smad2/3,and p-AKT were examined using Western blot.Furthermore,xenograft tumor in nude mice was used to evaluate the growth and metastasis of NCI-H446 cells in vivo.Results:Our results demonstrated that Flot1 is highly expressed in SCLC samples and that its expression correlates strongly with clinical stage,distant metastasis,and poor survival.The knockdown of Flot1 decreased the growth,migration,and invasiveness of SCLC cells and reversed EMT phenotype in vitro and in vivo,while enhanced Flot1 expression exhibited the opposite behavior.Gene expression profile analysis demonstrated that Flot1-regulated genes frequently mapped to the AKT and TGF-β-Smad2/3pathways.Our results further revealed that Flot1 affected the progression of SCLC via regulation of EMT progression.Conclusions:These findings indicated an oncogenic role of Flot1 via promoting EMT in SCLC and suggested its potential as a tumor marker and prognostic indicator.

关键词： Flotillin1 small cell lung cancer EMT TGF-β-smad2/3

长非编码RNA NRAV在泛癌中的表达和功能研究

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德州学院学报 2024年第2期40卷 1-8页

作者：黄平平李若冰胡维博单亚男许梦阳周百灵扈国栋德州学院山东省生物物理重点实验室生物物理研究院山东德州253023 乐陵市人民医院山东德州253600

LncRNA NRAV是近年来新发现的lncRNA分子,仅少数研究显示其表达水平与肺癌、胰腺导管腺癌的发展紧密相关,但NRAV在其它癌症中的表达情况和功能尚不清晰。通过生物信息学的方法分析NRAV在不同癌症中的差异表达情况及其与癌症患者生存期... 详细信息

LncRNA NRAV是近年来新发现的lncRNA分子,仅少数研究显示其表达水平与肺癌、胰腺导管腺癌的发展紧密相关,但NRAV在其它癌症中的表达情况和功能尚不清晰。通过生物信息学的方法分析NRAV在不同癌症中的差异表达情况及其与癌症患者生存期的关系,并以肝细胞癌(LIHC)作为重点研究对象,基于转录组数据筛选与NRAV共表达的mRNA并进行GO和KEGG功能富集分析,探究NRAV在LIHC中的调控机制与功能。结果显示,NRAV在7种癌症与癌旁组织中差异表达显著,其表达量与5种癌症患者的总生存期以及4种癌症患者的无病生存期显著相关。另外,筛选出的479个共表达靶基因主要富集在DNA修复、蛋白质结合、细胞周期等通路上。该文为进一步了解NRAV与泛癌尤其是肝细胞癌的发生发展关系提供初步依据。

关键词：长非编码RNA NRAV 泛癌肝细胞癌分子机制

Unraveling the regulatory network of flower coloration in soybean:Insights into roles of GmMYBA3 and GmMYBR1

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The Crop Journal 2024年第2期12卷 443-455页

作者： Ruifang Gao Yueqing Li yanan Wang Xiaotong shan Siqi Yang Ye Zhang Saiyu Ma Chunbao Zhang Jianchun Qin Li Wang Xiang Gao College of Plant Science Jilin UniversityChangchun 130062JilinChina Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics&Cytology Northeast Normal UniversityChangchun 130024JilinChina Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033JilinChina

Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.

关键词： Anthocyanin Flower color determination MBW complex MYB repressor Pleiotropic regulator

牙鲆感染迟钝爱德华氏菌的miRNA转录组分析

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天津师范大学学报（自然科学版） 2022年第2期42卷 37-45页

作者：山亚男郑津辉高虹天津师范大学生命科学学院天津300387 天津师范大学天津市动植物抗性重点实验室天津300387

为了探究牙鲆感染迟钝爱德华氏菌过程中miRNA的分子调控机制,以牙鲆头肾组织为研究材料,分析其感染迟钝爱德华氏菌后miRNA的差异表达情况.利用高通量测序平台(Illumina HiSeq)分别对感染迟钝爱德华氏菌3 h、6 h以及未感染的牙鲆头肾组织... 详细信息

为了探究牙鲆感染迟钝爱德华氏菌过程中miRNA的分子调控机制,以牙鲆头肾组织为研究材料,分析其感染迟钝爱德华氏菌后miRNA的差异表达情况.利用高通量测序平台(Illumina HiSeq)分别对感染迟钝爱德华氏菌3 h、6 h以及未感染的牙鲆头肾组织的RNA进行转录组测序,并对数据进行生物信息学分析.将测序结果与斑马鱼miRBase数据库进行比对,共鉴定出牙鲆成熟miRNA 537个.将感染组与对照组数据进行比对,得到12个感染组(3 h)差异显著表达的miRNA(2个显著上调,10个显著下调);32个感染组(6 h)差异显著表达的miRNA(20个显著上调,12个显著下调).对感染组差异显著表达的miRNA的靶基因进行GO seq分析,结果显示,感染组(3 h)的富集通路有2793条(P<0.05),包括2021个生物过程、315个细胞组分和457个分子功能;感染组(6 h)的富集通路有3048条(P<0.05),包括2235个生物过程、342个细胞组分和471个分子功能.KEGG分析结果显示,感染组(3 h)中获得71个富集通路(P<0.05),包括31个有机系统、17个环境信息处理、6个细胞过程、13个人类疾病和4个代谢通路;感染组(6 h)中获得69个富集通路(P<0.05),包括33个有机系统、18个环境信息处理、6个细胞过程、10个人类疾病和2个代谢通路.选取6个差异表达miRNA进行实时荧光定量PCR验证,结果显示,荧光定量结果与转录组测序结果趋势一致.总的来看,在牙鲆感染迟钝爱德华氏菌过程中,差异表达miRNA的靶基因参与的信号通路大部分跟生物过程以及细胞组分中的膜成分有关,生物过程中候选靶基因富集程度最高的GO条目是生物调节,富集程度较高的通路大多跟免疫调节以及分裂分化等生物学过程有关.

关键词：迟钝爱德华氏菌牙鲆 miRNA 转录组高通量差异表达miRNA

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动车组产品造修数据的分析应用

智慧轨道交通 2023年第1期60卷 11-14页

作者：单亚男李云鹏中车唐山机车车辆有限公司河北唐山064000 北京瑞思天创科技有限公司北京100176

随着国家高速铁路和城市轨道交通的快速发展,轨道交通装备的维修体制也在日益更新,现在已从传统的故障修和凭借经验制定的预防性维修,逐渐向状态修这种以可靠性为中心的维修体制迈进。目前,国内部分企业已经开始对高速动车组的失效数据... 详细信息

随着国家高速铁路和城市轨道交通的快速发展,轨道交通装备的维修体制也在日益更新,现在已从传统的故障修和凭借经验制定的预防性维修,逐渐向状态修这种以可靠性为中心的维修体制迈进。目前,国内部分企业已经开始对高速动车组的失效数据进行分析研究,以此来指导动车组的修程修制的优化和设计改进。但是,对于新造和高级修中记录的造修数据的利用还处于初步探索甚至是空白阶段。本文分析了造修数据的基本特点,列举了基于动车组造修数据的统计过程控制和性能退化分析的一般方法,论证了其可行性和必要性。并对造修数据收集工作存在的实际问题进行了阐述,提出了改进的建议。

关键词：动车组造修数据统计过程控制性能退化分析可靠性评估

宫腔观察吸引手术技术在剖宫产瘢痕妊娠治疗中应用的初探

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中华生殖与避孕杂志 2024年第1期44卷 73-76页

作者：史亚楠王晓高艳飞单淑芝江静石彬河北医科大学第二医院妇产科石家庄050000

目的了解宫腔观察吸引手术技术在剖宫产瘢痕妊娠(cesarean scar pregnancy,CSP)治疗中的应用效果及安全性。方法回顾性分析2019年4月至2022年6月期间于河北医科大学第二医院妇产科采用宫腔观察吸引手术技术作为初始治疗的7例CSP患者的... 详细信息

目的了解宫腔观察吸引手术技术在剖宫产瘢痕妊娠(cesarean scar pregnancy,CSP)治疗中的应用效果及安全性。方法回顾性分析2019年4月至2022年6月期间于河北医科大学第二医院妇产科采用宫腔观察吸引手术技术作为初始治疗的7例CSP患者的临床资料。所有患者均使用一次性摄像吸引管同步观察吸引,术前3 h放置一次性宫颈扩张器,术后3 d复查血β-人绒毛膜促性腺激素(human chorionic gonadotropin,hCG)及超声检查,必要时进行宫腔镜治疗。结果7例CSP患者中Ⅰ型3例,Ⅱ型4例,年龄为(33.9±4.7)岁,范围为27~40岁,停经时间范围为38~47 d,中位时间是43 d。所有患者手术过程均顺利,术中中位出血量30 mL,术后中位出血量20 mL,术后中位出血时间3 d,无子宫穿孔和术中大出血发生,术后所有患者血β-hCG均下降满意,6例复查超声未见明显异常,1例Ⅱ型患者因超声提示瘢痕部位强回声伴随丰富血流信号行宫腔镜电切术。结论宫腔观察吸引手术技术借助其内置于吸引管头端的高清摄像头,能真正做到观察与吸引同步,有望应用于停经≤47 d的Ⅰ型及部分Ⅱ型CSP的治疗。

关键词：剖宫产瘢痕妊娠宫腔观察吸引手术技术一次性摄像吸引管

持续质量改进的临床护理措施对重度子痫前期产妇妊娠结局影响

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中国计划生育学杂志 2024年第1期32卷 93-96,101页

作者：薛山张亚囡左青江苏省人民医院南京医科大学第一附属医院210000

目的:分析基于持续质量改进的临床护理措施对重度子痫前期(SPE)产妇妊娠结局的影响.方法:采用随机抽样的方式在本院2020年1-2022年12月收治的SPE患者120例作为研究对象,随机数字表法分为对照组和观察组各60例.对照组给予常规护理干预,... 详细信息

目的:分析基于持续质量改进的临床护理措施对重度子痫前期(SPE)产妇妊娠结局的影响.方法:采用随机抽样的方式在本院2020年1-2022年12月收治的SPE患者120例作为研究对象,随机数字表法分为对照组和观察组各60例.对照组给予常规护理干预,观察组给予基于持续质量改进的临床护理措施.对比分析两组分娩孕周、分娩方式、血压水平、孕产妇并发症发生率以及新生儿状态.结果:护理干预后两组分娩方式无差异(P>0.05),分娩孕周观察组(37.8±2.8周)高于对照组(36.1±2.9周)(P<0.05);两组血压水平均降低且观察组(126.8±14.0mmHg、88.5±6.8mmHg)均低于对照组(135.5±13.4mmHg、92.5±6.3mmHg).观察组孕产妇并发症发生率(6.7%)低于对照组(20.2%),新生儿Apgar评分(7.8±1.0分)高于对照组(7.1±1.3分),新生儿并发症发生率(11.7%)低于对照组(26.7%)(均P<0.05).结论:基于持续质量改进的临床护理措施可降低SPE患者的血压水平,改善妊娠结局.

关键词：重度子痫持续质量改进护理干预血压妊娠结局并发症