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Phylogenetic Relationships in Genus Arachis Based on SSR and AFLP Markers

Agricultural Sciences in China 2008年第4期7卷 405-414页

作者： TANG Rong-hua ZhuaNG Wei-jian GAO Guo-qing HELiang-qiong HAN Zhu-qiang shan shi-hua JIANG Jing LI Yang-rui Cash Crops Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 P.R.China Crop Science School Fujian Agriculture and Forestry University Fuzhou 350002 P.R. China Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Guangxi Academy of Agricultural Sciences Nanning 530007 P.R.China shangdong Peanut Research Institute Qingdao 266100 P.R.China

Fourteen wild species of different sections in the genus Arachis and 24 accessions of the AABB allotetraploid A. hypogaea （cultivated peanut） from several countries which belong to different botanical varieties, were analyzed by SSR and AFLP marker systems. The assay-units per system needed to distinguish among all the tested accessions were at least five for SSR or two for AFLP. The genetic distance detected by the SSR markers ranged from 0.09 to 0.95, and the mean was 0.73; and the genetic distance detected by the AFLP markers ranged from 0.01 to 0.79 with an average of 0.42. All the tested peanut SSR primer pairs were multilocus ones, and the amplified fragments per SSR marker in each peanut genome ranged from 2 to 15 with the mean of 4.77. The peanut cultivars were closely related to each other, and shared a large numbers of SSR and AFLP fragments. In contrast, the species in the genus Arachis shared few fragments. The results indicated that the cultivated peanut （A. hypogaea L.） varieties could be partitioned into two main groups and four subgroups at the molecular level, and that A. duranensis is one of the wild ancestors of A. hypogaea. The lowest genetic variation was detected between A. cardenasii and A. batizocoi, and the highest was detected between A. pintoi and the species in the section Arachis. The relationships among the botanical varieties in the cultivated peanut （A. hypogaea L.） and among wild species accessions in section Arachis and those in other sections in the genus Arachis were discussed.

关键词： Arachis SSR AFLP genetic diversity phylogenetic relationship

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Research of Seed Testa Structure and Storage Material of Peanut Germplasm with Different Resistance to A. flavus

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Agricultural Sciences in China 2006年第6期5卷 478-482页

作者： shan shi-hua WANG Hai-xia LI Chun-juan WAN Shu-bo LIU Hong-tao JIANG Guo-yong shandong Peanut Research Institute Qingdao 266100 P.R.China Life Institute Laiyang Agricultural College Laiyang 265200 P.R. China

There was an obvious relationship between seed testa structure, storage material and resistance to A. flavus of peanut. Results showed that seed testa of peanut germplasm with high resistance （HR） to A. flavus infection had thicker wax layer, integrated and tight epidermis layer, regular vascular tissue range. However, the seed testa of peanut germplasm with high sensitivity （HS） to A. flavus had the reverse results, and results of those with medium resistance （MR） to A. flavus lay in between, but changes of testa thickness were not significant among different resistance kinds. Results also showed that some seed storage materials were closely related with resistance potential to A. flavus. It seemed that varieties with higher resistance to A. flavus had higher oleic acid and protein content, lower linoleic acid and fat content. Content of palm acid, total sugar and VE did not show positive relationship with the resistance to A. flavus.

关键词： peanut germplasm resistance to A. flavus seed testa structure and storage material

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Influence of Anthesis Date and Boll Branch Position on Qualities and Super-molecular Structure of Cotton Fiber

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Agricultural Sciences in China 2002年第6期1卷 636-641页

作者： shan shi-hua, shi Pei, SUN Xue-zhen, ZHOU Zhi-guo and BIAN Dong-cai(Agronomy Department of shandong Agricultural University, Taian 271018 , P. R . China Tianjin Institute of Textile , Tianjin 300160 , P.R. China ) Agronomy Department of shandong Agricultural University Taian 271018 P. R. China Tianjin Institute of Textile Tianjin 300160 P. R. China

The influences of the anthesis date and the fruiting branch positions on parameters of cotton fiber quality and super-molecular structure were studied. Fiber quality parameters, such as cotton fiber length, micronair, maturity and fiber strength deteriorated with postponing the anthesis date and decreasing temperature. When anthesis happened on the same date, the fruiting branch positions affected the fiber quality parameters, and the fiber quality parameters of the lower portion turned out to be higher than that of the upper portion, although the difference was insignificant. The super-molecular structure of the lower portion turned out to be superior to that of the upper portion at the early anthesis date and high temperature, which is in accordance with what was mentioned above, but the fruiting branch positions did not affect the impact of the anthesis date on the super-molecular structure.

关键词： Cotton Anthesis date Fruiting branch positions Fiber qualities Super-molecular structure

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基于GSPN的舰载服务器系统可靠性建模及分析

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火力与指挥控制 2015年第5期40卷 57-60,64页

作者：李展单士华黄河科技学院郑州450063

提出一种基于广义随机Petri网(Generalized Stochastic Petri Net,GSPN)的舰载服务器系统可靠性分析方法。深入分析舰载服务器系统故障模式的基础上,建立冗余服务器子系统的GSPN模型和共享数据盘子系统的GSPN模型,进而综合得到舰载服务... 详细信息

提出一种基于广义随机Petri网(Generalized Stochastic Petri Net,GSPN)的舰载服务器系统可靠性分析方法。深入分析舰载服务器系统故障模式的基础上,建立冗余服务器子系统的GSPN模型和共享数据盘子系统的GSPN模型,进而综合得到舰载服务器系统的全局GSPN模型,有效模拟了舰载服务器系统的动态行为。仿真实验验证了所提方法的有效性,为舰载服务器系统的分析与设计提供理论参考。

关键词：广义随机Petri网舰载服务器系统可靠性可用度

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ImSpiRE: image feature-aided spatial resolution enhancement method

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Science China Life Sciences 2025年第1期 272-283页

作者： Yuwei hua Yizhi Zhang Zhenming Guo shan Bian Yong Zhang State Key Laboratory of Cardiovascular Diseases and Medical Innovation Center Institute for Regenerative MedicineDepartment of NeurosurgeryShanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji University

The resolution of most spatially resolved transcriptomic technologies usually cannot attain the single-cell level, limiting their applications in biological discoveries. Here, we introduce ImSpiRE, an image feature-aided spatial resolution enhancement method for in situ capturing spatial transcriptome. Taking the information stored in histological images, ImSpiRE solves an optimal transport problem to redistribute the expression profiles of spots to construct new transcriptional profiles with enhanced resolution, together with extending the gene expression profiles into unmeasured regions. Applications to multiple datasets confirm that ImSpiRE can enhance spatial resolution to the subspot level while contributing to the discovery of tissue domains, signaling communication patterns, and spatiotemporal characterization.

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温度对棉纤维品质性状的影响

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华北农学报 2000年第4期15卷 120-125页

作者：单世华孙学振周治国施培山东农业大学农学系山东泰安271018

随温度降低纤维伸长最大值降低 ,伸长期延长 ;夜间温度对纤维伸长的影响大于日均温。纤维断裂比强度随铃龄增大而升高 ;相同铃龄随温度下降断裂比强度降低。随纤维发育 ,纤维成熟度升高 ;随温度降低 ,成熟度随铃龄增大增幅降低 ,相同铃... 详细信息

随温度降低纤维伸长最大值降低 ,伸长期延长 ;夜间温度对纤维伸长的影响大于日均温。纤维断裂比强度随铃龄增大而升高 ;相同铃龄随温度下降断裂比强度降低。随纤维发育 ,纤维成熟度升高 ;随温度降低 ,成熟度随铃龄增大增幅降低 ,相同铃龄随温度下降成熟度值呈下降趋势。细度随纤维发育逐渐减小 ,全铃期夜均温 16 7℃和≥ 15℃有效积温 1142 9℃是细度变化临界点 ,低于该温度时细度随纤维发育降低缓慢 ;相同铃龄随温度下降呈升高趋势。

关键词：温度棉花纤维品质作用机理生态区划播期

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AhRLK1,a CLAVATA1-like leucine-rich repeat receptor-like kinase of peanut,confers increased resistance to bacterial wilt in tobacco

AhRLK1,a CLAVATA1-like leucine-rich repeat receptor-like kin...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： ZHANG Chong CHEN hua ZhuaNG Rui-rong DENG Ye CAI Tie-cheng WANG Shuai-yin LIU Qin-zheng TANG Rong-hua shan shi-hua PAN Rong-long ZhuaNG Wei-jian State key laboratory of ecological pest control for Fujian and Taiwan crops Fujian Agriculture and Forestry University Key Laboratory of Crop Molecular and Cell Biology Fujian Agriculture and Forestry University Cash Crops Research Institute Guangxi Academy of Agricultural Sciences shandong Peanut Research Institute Department of Life Science and Institute of Bioinformatics and Structural Biology College of Life ScienceNational Tsing Hua University

Bacterial wilt caused by Ralstonia solanacearum is a devastating disease that infects hundreds of plant *** factors involved in disease resistance and pathogenesis remain poorly *** up regulated and leucine-rich repeat receptor-like kinase characterized as CLAVATA1 and named AhRLK1 was obtained by microarray analysis in response to *** in *** contained presumably,a signal peptide,ten leucine-rich repeat(LRR) domains and conserved motifs of intracellular *** subcellular localization,the AhRLKl protein was visualized only in plasma *** inoculation with ***,AhRLK1 was constantly up regulated in the susceptible variety Xinhuixiaoli but showed little changed in the resistant cultivar Yueyou *** hormones,including salicylic acid,abscisic acid,methyl jasmonate and ethephon induced expression,but expression was completely down regulated under cold and drought *** overexpression provoked a hypersensitive response(HR) in Nicotiana benthamiana following ***,the resistance to *** increased significantly in transgenic overexpression *** contrast,most representative defense-responsive genes in defence related pathways such as NtHIN1,NtPR2,NtLOX1 and NtACS6,were considerably up regulated in the AhRLK1 transgenic ***,EDS1 and PAD4 in R gene signal were also up regulated in transgenic plants,but NDR1 and NPR1 genes were down ***,we suggest that AhRLK1 increases the defense response to *** via HR and hormone defense signalling,associated with EDS1 pathway of R gene *** results provide new understanding of CLV1 function and will contribute to genetic enhancement of peanut.

关键词： peanut bacterial wilt microarray analysis AhRLK1

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Genetic map construction and widely distributed meiotic recombination hot spots detected by SLAF in peanut(Arachis hypogaea L.)

Genetic map construction and widely distributed meiotic reco...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： WANG Xiao-hua XU Ping YIN Liang REN Yan LI Shuang-ling shi Yan-mao CHI Xiao-yuan YU Tian-yi shan shi-hua HE Guo-hao Manish K PANDey Rajeev K Varshney YUAN Mei Key Laboratory of Peanut Biology and Genetic Improvement Ministry of AgricultureShandong Peanut Research Institute Tuskegee University International Crops Research Institute for Semi-Arid Tropics(ICRISAT) Greater Hyderabad

Recombination hot spots,caused by meiosis,are considered playing crucial roles in crop domestication and *** peanut is one of the most staple,rich-source of oil and protein ***,no direct scale of recombination events and hot spots have been estimated for peanut *** examine the scale of recombination events and hot spots in peanut,we constructed a genetic map using a recombinant inbred line(RIL) population including 200 lines with 605 631 markers detected using the specific-locus amplified fragment sequencing(SLAF-seq) approach and 49 natural cultivars with 61 942 insertion-deletion(InDels) markers detected using the transcriptome sequencing,*** precise integrated map comprised 4 837 SLAF markers with a total genetic length of 2 915.46 cM,a density of 1.66 markers per *** average of 30.0 crossover(c.2.06 cM/Mb) events was detected per F peanut plant,which is similar to the values in Arabidopsis and *** crossover events showed uneven distribution among B sub-genome(with average of 2.32) and A sub-genome(with average of 1.85).There were 4.34%and 7.86%of the genome contained large numbers of crossovers(>50 cM/Mb) along chromosomes in F population and natural population,*** density of crossover events region called recombination hot spots,showed negative relationship to marker haplotypes conservative region but positive to heatmap of *** genes located within the recombination hot spot regions by gene ontology(GO) categories showed responses to environmental stimuli,suggesting that recombination plays a crucial role in peanut adaptation to changing environments.

关键词： Arachis hypogaea L. genetic map meiotic recombination hot spots SLAF-seq(specific-locus amplified fragment sequencing)

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核糖体蛋白L41与黄曲霉抗性的关系

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华北农学报 2011年第6期26卷 16-19页

作者：严海燕宗成志马国华单世华中南民族大学生命科学学院湖北武汉430074 山东省花生研究所山东青岛266100

已知与编码核糖体蛋白L41(RPL41)的CD399094相似的基因序列在黄曲霉敏感品种发育中种皮中上调表达。从花生黄曲霉抗性品种J11中克隆了相似基因。通过分析比较来自开放数据库中黄曲霉抗性和敏感品种花生发育中种子表达的RPL41序列,并对... 详细信息

已知与编码核糖体蛋白L41(RPL41)的CD399094相似的基因序列在黄曲霉敏感品种发育中种皮中上调表达。从花生黄曲霉抗性品种J11中克隆了相似基因。通过分析比较来自开放数据库中黄曲霉抗性和敏感品种花生发育中种子表达的RPL41序列,并对黄曲霉抗性和敏感品种果实不同发育时期和部位及叶片用荧光定量PCR做进一步的分析,研究在不同花生品种中核糖体蛋白L41与黄曲霉抗性的关系。结果表明,发育中种子表达的RPL41在抗性品种中多于敏感品种,而且多出的EST序列与在敏感品种中表达的不同,可能是特异地在抗性品种中表达。RPL41在抗性品种中发育中果实的果荚和子叶以及叶片上调表达,可能与该特定类型的RPL41 est的专一表达以及与黄曲霉的抗性有关。

关键词：花生黄曲霉核糖体蛋白L41:果实发育子叶

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P2Y1 receptor in Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 440-453页

作者： shan Luo Yifei Wang Tatsuhiro Hisatsune Department of Integrated Biosciences The University of TokyoKashiwaJapan

Alzheimer’s disease is the most frequent form of dementia characterized by the deposition of amyloid-beta plaques and neurofibrillary tangles consisting of hyperphosphorylated *** amyloid-beta plaques has been a primary direction for developing Alzheimer’s disease treatments in the last ***,existing drugs targeting amyloid-beta plaques have not fully yielded the expected results in the clinic,necessitating the exploration of alternative therapeutic *** evidence unravels that astrocyte morphology and function alter in the brain of Alzheimer’s disease patients,with dysregulated astrocytic purinergic receptors,particularly the P2Y1 receptor,all of which constitute the pathophysiology of Alzheimer’s *** receptors are not only crucial for maintaining normal astrocyte function but are also highly implicated in neuroinflammation in Alzheimer’s *** review delves into recent insights into the association between P2Y1 receptor and Alzheimer’s disease to underscore the potential neuroprotective role of P2Y1 receptor in Alzheimer’s disease by mitigating neuroinflammation,thus offering promising avenues for developing drugs for Alzheimer’s disease and potentially contributing to the development of more effective treatments.

关键词： astrocytes neuroinflammation P2Y1 receptor purinergic receptor

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