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Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean:Adult-Plant Resistance and Resistance to Seed Coat Mottling

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Agricultural Sciences in China 2010年第1期9卷 11-18页

作者： WANG Kun LI Wen-fu ZHANG Lei LIU Chun-yan ZHU Xiao-shuang CHEN Qing-shan HU Guo-hua Agriculture Academy Northeast Agricultural University/Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province/ National Research Center of Soybean Engineering and Technology/Soil Fertility and Environmental Resources Institute Heilongjiang Academy of Agricultural Sciences Harbin 150090 P.R.China

Soybean mosaic virus （SMV） could lead to adult-plant system diseases, and cause mottling of soybean seeds. Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 （susceptible）x Dongnong 8143 （resistant） and Dongnong 3C628 （susceptible）× Tie 6915 （resistant）. Simple sequence repeat （SSR） markers with bulked segregation analysis （BSA） were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel＇s genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat 229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335. The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection （MAS）.

关键词： soybean SMV seed coat mottling genetic analysis SSR gene mapping

Isolation and Characterization of a Putative Class E Gene from Taihangia rupestris

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Journal of Integrative Plant Biology 2007年第3期49卷 343-350页

作者： Yong-Qiang Wang Hui-Yu Tian Xiao-Qiu Du shan-hua Lü Wen-Liang Lu Kang Chong Zheng Meng Key Laboratory of Photosynthesis and Environmental Molecular Physiology the Chinese Academy of Sciences Beijing 100093 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Studies In model plants showed that SEPALLATA （SEP） genes are required for the Identification of floral organs and the determination of floral meristems In Arabidopsis. In this paper a SEP homolog, TrSEP3, was Isolated from a China-specific species, Taihangla rupestrisi Yü et LI. Phylogenetlc analysis showed that the gene belongs to the SEP3-clade of SEP （previous AGL2） subfamily. In situ hybridization was used to reveal the potential functional specification, and the results showed that TrSEP3 expression was first observed in floral meristems and then confined to the floral primordla of the three inner whorls. In the matured flower, TrSEP3 was strongly expressed In the tips of pistils and weak In stamens and petals. The evolution force analysis shows that TrSEP3 might undergo a relaxed negative selection. These results suggested that TrSEP3 may not only function In determining the identity of floral merlstems and the primordia of three inner whorls, but also function In matured reproductive organs.

关键词： class E gene MADS-box selection pressure Taihangia rupestris

Preparation,Characterization and Properties of Two New Lanthanide(Ⅲ)-5-(2-pyridyl)tetrazolate Complexes

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Chemical Research in Chinese Universities 2010年第6期26卷 866-870页

作者： ZHOU Yun-shan XU Dong-hua ZHANG Li-juan State Key Laboratory of Chemical Resource Engineering College of Science Beijing University of Chemical Technology Beijing 100029 P. R. China

Two new mononuclear lanthanide（Ⅲ） complexes Ln（pytz）3（H2O）3·（H2O）3.5[Ln=Tb（1）;Eu（2）;Hpytz=5-（2-pyridyl）tetrazole] were synthesized by reacting Hpytz with the corresponding lanthanide（Ⅲ） ions and *** single crystal X-ray diffraction analysis reveals that complexes 1 and 2 are isostructural and the lanthanide（Ⅲ） ions in both complexes 1 and 2 are nine-coordinated,with three oxygen atoms of three coordination water molecules and six nitrogen atoms of three pytz ligands,forming a monocapped square *** hydrogen bonds exist,resulting in a three-dimensional supramolecular network structure by hydrogen-bonds in both complexes 1 and 2,*** 1 exhibits typical green fluorescence of Tb（Ⅲ） ion and complex 2 red fluorescence of Eu（Ⅲ） ion,in solid state at room temperature.

关键词： Crystal structure Fluorescence Lanthanide(Ⅲ) complex

Responses of Ryegrass(Lolium perenne L.) Grown in Mudflats to Sewage Sludge Amendment

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Journal of Integrative Agriculture 2014年第2期13卷 426-433页

作者： BAI Yan-chao GU Chuan-hui TAO Tian-yun ZHU Xiao-wen XU Yi-ran shan Yu-hua FENG Ke College of Environmental Science and Engineering Yangzhou University Department of Geology Appalachian State University

Sewage sludge amendment （SSA） is an alternative waste disposal technique and a potential way to increase fertility of mudflats for crop growth. The present study aimed to assess the suitability of SSA by assessing the nitrogen （N） and phosphorous （P） uptakes, heavy metal accumulation, growth, biomass, and yield response of ryegrass （Lolium perenne L.） at 0, 30, 75, 150, and 300 t ha-1 SSA rates at various growth stages. The results showed that the highest biomass ofryegrass at seedling and vegetative stages were at 300 and 150 t ha-1 SSA rate, respectively. The increments of ryegrass yield at reproductive stage at 30, 75, 150, and 300 t ha1 SSA rates were 98.0, 122.6, 88.1, and 61.2%, compared to unamended soil. N and P concentrations in ryegrass increased with increasing SSA rates at all stages except N and P in roots dropped significantly at 300 t ha-1 rate at vegetative stage. The metal concentration for Mn, Cu, Zn, Ni, Cd, Cr, and Pb in shoot of ryegrass at 300 t ha-1 S SA rate increased by 0.63-, 2.34-, 15.02-, 0.97-, 10.00-, 0.01- and 1.13-fold, respectively, compared to unamended soil. However, heavy metal concentrations in shoot of ryegrass were lower than the standard for forage products in China. The study suggested that sewage sludge amendment in mudflat soils might be feasible. However, the impacts of sludge application on edible crop plants and soil environment need further investigations.

关键词： sewage sludge mudflat soil ryegrass growth metals

Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods

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Chinese Medical Sciences Journal 2013年第1期28卷 50-54页

作者： Tao Wang hua-shan Zhao Qiu-ling Zhang Chang-lin Xu Chang-bai Liu Institute of Molecular Biology China Three Georges University Third Clinical Medical School China Three Georges University First Clinical Medical School China Three Georges University Department of Neurosurgery Gezhouba Central HospitalChina Three Georges University Yichang City Central Hospital China Three Georges University

Induced pluripotent stem （iPS） cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors （Oct3/4, Sox2, Klf4, and c-Myc）. The technique was quickly re- produced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic po- tential in animal models of sickle ceil anemia and Parkinson＇s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.

关键词： induced pluripotent stem cells non-viral methods transcription factor re-generative medicine

Phylogenetic Relationships in Genus Arachis Based on SSR and AFLP Markers

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Agricultural Sciences in China 2008年第4期7卷 405-414页

作者： TANG Rong-hua ZhuaNG Wei-jian GAO Guo-qing HELiang-qiong HAN Zhu-qiang shan Shi-hua JIANG Jing LI Yang-rui Cash Crops Research Institute Guangxi Academy of Agricultural Sciences Nanning 530007 P.R.China Crop Science School Fujian Agriculture and Forestry University Fuzhou 350002 P.R. China Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Guangxi Academy of Agricultural Sciences Nanning 530007 P.R.China shangdong Peanut Research Institute Qingdao 266100 P.R.China

Fourteen wild species of different sections in the genus Arachis and 24 accessions of the AABB allotetraploid A. hypogaea （cultivated peanut） from several countries which belong to different botanical varieties, were analyzed by SSR and AFLP marker systems. The assay-units per system needed to distinguish among all the tested accessions were at least five for SSR or two for AFLP. The genetic distance detected by the SSR markers ranged from 0.09 to 0.95, and the mean was 0.73; and the genetic distance detected by the AFLP markers ranged from 0.01 to 0.79 with an average of 0.42. All the tested peanut SSR primer pairs were multilocus ones, and the amplified fragments per SSR marker in each peanut genome ranged from 2 to 15 with the mean of 4.77. The peanut cultivars were closely related to each other, and shared a large numbers of SSR and AFLP fragments. In contrast, the species in the genus Arachis shared few fragments. The results indicated that the cultivated peanut （A. hypogaea L.） varieties could be partitioned into two main groups and four subgroups at the molecular level, and that A. duranensis is one of the wild ancestors of A. hypogaea. The lowest genetic variation was detected between A. cardenasii and A. batizocoi, and the highest was detected between A. pintoi and the species in the section Arachis. The relationships among the botanical varieties in the cultivated peanut （A. hypogaea L.） and among wild species accessions in section Arachis and those in other sections in the genus Arachis were discussed.

关键词： Arachis SSR AFLP genetic diversity phylogenetic relationship

Numerical simulation for determining three zones in the goaf at a fully-mechanized coal face

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Journal of China University of Mining and Technology 2008年第2期18卷 199-203页

作者： HE Xiao-gang ZHANG Ren-wei PEI Xiao-dong SUN Yong TONG Bo-gen huaNG hua-shan School of Energy Sources ＆ Safety Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China Zhuxianzhuang Coal Mine of huaibei Mining Co. Ltd in Anhui Suzhou Anhui 234111 China

According to the theory of ventilation network, a model for a filtration flow field in goaf was built and simulation soft- ware for determining the three zones in goaf has been developed. This software uses no-gap-connection between Visual Basic (VB) and Excel to exchange data, uses Component Object Model (COM) component of MATLAB to plot charts of the three zones and to export the corresponding coordinates of the isolines. An example shows that this software is convenient and simple. By using it, the three zones can be easily determined. The software is convenient in studies and analyses of the three zones in goaf.

关键词： fully-mechanized coal face spontaneous combustion filtration flow field limit of rate of speed

Elevated preoperative CA125 is associated with poor survival in patients with metastatic colorectal cancer undergoing primary tumor resection:a retrospective cohort study

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Gastroenterology Report 2022年第1期10卷 386-394页

作者： Jun-hua huang hua-shan Liu Tuo Hu Zong-Jin Zhang Xiao-Wen He Tai-Wei Mo Xiao-Feng Wen Ping Lan Lei Lian Xian-Rui Wu Department of Colorectal Surgery The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Gastric Surgery The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China

Background The impact of the preoperative carbohydrate antigen 125(CA125)level on the survival of metastatic colorectal cancer(CRC)patients undergoing primary tumor resection(PTR)remains *** aim of this study was to assess the prognostic value in overall survival(OS)and cancer-specific survival(CSS)between patients with and without an elevated preoperative CA125 *** All metastatic CRC patients receiving PTR between 2007 and 2017 at the Sixth Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)were retrospectively *** and CSS rates were compared between patients with and without elevated preoperative CA125 *** Among 326 patients examined,46(14.1%)exhibited elevated preoperative CA125 levels and the remaining 280(85.9%)had normal preoperative CA125 *** with elevated preoperative CA125 levels had lower body mass index,lower preoperative albumin level,lower proportion of preoperative chemotherapy,higher carcinoembryonic antigen and carbohydrate antigen 19–9(CA19–9)levels,poorer differentiation,and more malignant histopathological type than patients with normal preoperative CA125 *** addition,patients with elevated preoperative CA125 levels exhibited more advanced pathological T and N stages,more peritoneal metastasis,and more vessel invasion than patients with normal preoperative CA125 ***,the primary tumor was more likely to be located at the colon rather than at the rectum in patients with elevated CA125 *** OS and CSS rates in patients with elevated preoperative CA125 levels were significantly lower than those in patients with normal preoperative CA125 *** Cox regression analysis revealed that an elevated preoperative CA125 level was significantly associated with poor prognosis in metastatic CRC patients undergoing *** hazard ratio(HR)in OS was 2.36(95%confidence interval[CI],1.67–3.33,P<0.001)and the HR in CSS was 2.50(95%CI,1.77–3.55,P<0.001).The survival anal

关键词： metastatic colorectal cancer primary tumor resection CA125 prognosis survival

Structural origin for composition-dependent nearest atomic distance in Cu–Zr metallic glass

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Chinese Physics B 2023年第11期32卷 545-549页

作者：张驰刘华山彭海龙 School of Materials Science and Engineering Central South UniversityChangsha 410083China

We systematically investigate the structures of Cu–Zr metallic glass(MG) by varying the Cu concentration in classic molecular-dynamics simulation. From the pair distribution functions(PDFs), it is found that the nearest atomic distance between Zr atom and Zr atom increases significantly after adding Cu, which is related to the composition-dependent coordination behavior between Cu atom and Zr atom in the nearest neighbors. The portion of PDF related to the nearest connection is decomposed into the contributions from quadrilateral structure, pentagonal structure, hexagonal structure,and heptagonal bipyramid structure. Although the population of denser structures, i.e. 5-, 6-, and 7-number sharing ones,increases with Cu addition increasing, the connection distances between the central atoms in all these bipyramids increase for Zr–Zr pairs, leading to the expansion of Zr–Zr nearest atomic distance. These results unveil the effect of the interplay between chemical interaction and geometric packing on the atomic-level structure in Cu–Zr metallic glasses.

关键词： metallic glass structure molecular dynamics