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Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)

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Journal of Cotton Research 2024年第1期7卷 90-100页

作者： BASHIR Samina YAQOOB Amina BASHIR Rohina BUKHARI Shanila shahid naila AZAM Saira BAKHSH Allah HUSNAIN Tayyab shahid Ahmad Ali RAO Abdul Qayyum Centre of Excellence in Molecular Biology University of the PunjabLahore 53700Pakistan Department of Botany Kinnaird College for Women UniversityLahore 54000Pakistan Department of Botany Government College UniversityFaisalabad 38000Pakistan Institute of Botany University of the PunjabLahore 54590Pakistan.

Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like *** present study aimed to investigate the expression of chemically synthesized barley ch1 and chi2 genes in cotton(Gossypium hirsutum)through Agrobacterium-mediated ***-five putative transgenic cotton plants were obtained,out of which fifteen plants successfully survived and were shifted to the *** gene-specific primers,amplification of 447 bp and 401 bp fragments confirmed the presence of the ch1 and chi2 genes in five transgenic cotton plants of the T0 *** five plants were further evalu-ated for their mRNA expression *** T0 transgenic cotton plants with the highest mRNA expression level and better yield performance in field,were selected to raise their subsequent *** The T1 cotton plants showed the highest mRNA expression levels of 3.5-fold in P10(2)for the ch1 gene and 3.7-fold in P2(1)for the chi2 *** in situ hybridization(FISH)confirmed a single copy number of ch1 and chi2(hemizygous)on chromosome ***,the efficacy of transgenes on whitefly was evaluated through an insect bioassay,where after 96 h of infestation,mortality rates of whitefly were calculated to be 78%–80%in transgenic cotton *** number of eggs on transgenic cotton plants were calculated to be 0.1%–0.12 per plant compared with the non-transgenic plants where egg number was calculated to be 0.90–1.00 per *** Based on these findings,it can be concluded that the chemically synthesized barley chitinase genes(ch1 and chi2)have the potential to be effective against insects with chitin exoskeletons,including *** transgenic cotton plants expressing these genes showed increased resistance to whiteflies,resulting in reduced egg numbers and higher mortality rates.

关键词： Chitinase Cotton White fly Transgene Bioassay

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Phytochemical analysis, antioxidant, acetylcholinesterase, and α-amylase inhibitors from Hippeastrum hybridum extract

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Journal of Traditional Chinese Medicine 2024年第3期44卷 496-504页

作者： naila Sher Mushtaq Ahmed Nadia Mushtaq Department of Biotechnology University of Science and Technology Department of Botany University of Science and Technology

OBJECTIVE:To investigate the effects of Hippeastrum hybridum（HH） as a free radical scavenger,and an inhibitor of the two enzymes i-e Alpha-amylase（α-amylase） and acetylcholinesterase（AChE）.METHODS:In this study,HH plant was preliminary analyzed for phytochemical screening and then tested for its antioxidant,anti-α-amylase,and anti-AChE efficiency via standard ***:Phytochemical analysis shows the existence of different compounds;while Coumarins and quinones were *** total phenolic,flavonoid,and tannins content were found to be（78.52±0.69） mg GAE/g,（2.01±0.04） mg RUE/g,and（58.12±0.23） mg TAE/g of plant extract respectively.28.02%±0.02% alkaloid and 2.02%±0.05% saponins were present in the HH *** HH extract showed the anti-oxidant property with IC50（50%inhibition） of（151.01±0.13）(HH),（79.01±0.04）(Ascorbic acid) for ferric reducing,（91.48±0.13）(HH),（48.02±0.11）(Ascorbic acid) against Ammonium molybdenum,（156.02±0.31）(HH),（52.38±0.21）(Ascorbic acid) against DPPH,136.01±0.21（HH）,52.02±0.31（Ascorbic acid） against H2O2,and 154.12±0.03（HH）,（40.05±0.15）(Ascorbic acid)μg/mL against ABTS *** analysis indicated that HH caused a competitive type of inhibition of α-amylase(Vmaxremained constant and Km increases from 10.65 to84.37%) while Glucophage caused the un-competitive type of inhibition i-e both Km and Vmaxdecreased from40.49 to 69.15%and 38.86 to 69.61%*** Ki,（inhibition constant）;KI,（dissociation constant）,Km,（Michaelis-Menten constant）,and IC50 were found to be62,364,68.1,and 38.08±0.22 for HH and 12,101.05,195,34.01±0.21 for ***,HH causes an anon-competitive type of inhibition of AChE i-e Km remains constant while Vmaxdecreases from 60.5%to74.1%.The calculated Ki,KI,Km,and IC50 were found to be 32,36.2,0.05,and 18.117±***:From the current results,it is concluded that HH extract contains bioactive compounds,and could be a good alternative to controlling oxidants,Alzheimer‘s and T

关键词： Hippeastrum hybridum acetylcholinesterase alpha-amylase antioxidant phytochemicals

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Combined Application of Biostimulants and EDTA Improved Wheat Productivity under Cadmium Stress

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1647-1665页

作者： Abida Aziz Shafiqa Bano Mubshar Hussain Muhammad Farooq Azhar Ghulam Yasin naila Hadayat Iqra Arooj Abeer Hashem Ajay Kumar Elsayed Fathi Abd_Allah Qamar uz Zaman Department of Botany The Women UniversityMultan54500Pakistan Institute of Agronomy Bahauddin Zakariya UniversityMultan60800Pakistan Department of Forestry and Range Management Bahauddin Zakariya UniversityMultan60800Pakistan Department of Botany Division of Science&TechnologyUniversity of EducationLahore54770Pakistan Department of Microbiology&Molecular Genetics The Women UniversityMultan54500Pakistan Botany and Microbiology Department College of ScienceKing Saud UniversityRiyadh201313Saudi Arabia Amity Institute of Biotechnology Amity UniversityNoida201313India Plant Production Department College of Food and Agricultural SciencesKing Saud UniversityRiyadh11451Saudi Arabia School of Agriculture Yunnan UniversityKunmingYunan650504China Department of Environmental Sciences The University of LahoreLahore54590Pakistan

Wheat(Triticum aestivum L.)exhibits a greater capacity for cadmium(Cd)absorption compared to other cereal crops,leading to elevated daily Cd intake,and posing a signiﬁcant threat to public *** the mitigation of Cd stress in sustainable and environmentally friendly way,a pot study was designed by using exogenous application of various biostimulants,i.e.,Nigella sativa and Ocimum sanctum extracts:0%,10%,and 20%in combination with the chelating agent ethylenediaminetetraacetic acid(EDTA)using 0 and 5 mg kg^(-1) under various levels of Cd stress(i.e.,0,5,10,and 15 mg kg^(-1) soil).Results revealed that Cd stress signiﬁcantly reduced the seed emergence,growth,root,and allometric characters and yield-related parameters of wheat *** most observable reduc-tion was documented in wheat plants exposed to a higher Cd concentration(15 mg kg^(-1)),followed by the lower Cd level(control).The combined application of bio-stimulants and EDTA minimized the negative impacts of Cd *** highest increase in seedling emergence(5.44%),leaf area(50.60%),number of tillers(31.02%),grain yield per plant(24.28%),biological yield(13.97%),and decrease in Cd levels in grains(40%)was noticed where 20%foliar application of *** and 10%of *** biostimulants were done using 5 mg kg^(-1) of soil-applied *** intervention demonstrated a notable reduction in Cd-induced negative effects,highlighting the potential of these substances in promoting sustainable wheat cultivation in contaminated ***,it is an eco-friendly and approachable method at theﬁeld level able to ensure food safety.

关键词： Abiotic stress allometric characters antioxidant activity crop yield seed emergence grain yield

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Abnormal ACT in a Patient with Prekallikrein Deficiency Undergoing Cardiopulmonary Bypass

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Open Journal of Anesthesiology 2024年第5期14卷 145-149页

作者： Maria R. Fuertes naila Ahmad Orlando Perez-Franco Andrew Oster Marion Svendrowski Pin Yue Department of Anesthesiology and Critical Care Saint Louis University School of Medicine St. Louis MO USA

Prekallikrein deficiency is a disorder that often remains undiagnosed. Prekallikrein activates factor XII, which initiates the intrinsic coagulation pathway. Prekallikrein deficiency results in prolonged Partial Thromboplastin Time and Activated Clotting Time in absence of anticoagulants or active bleeding. This case report describes the anesthesia management of a patient with Prekallikrein deficiency who underwent cardiac surgery with Cardiopulmonary Bypass for correction of a congenital cardiac malformation. We highlight the importance of understanding the different tests available for the diagnosis of coagulation factors deficiency during administration of heparin in the setting of cardiovascular procedures under general anesthesia.

关键词： Prekallikrein Deficiency Fletcher Factor Deficiency Cardiopulmonary Bypass Congenital Cardiac Malformation

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Generalized Coherent States for Position-Dependent Effective Mass Systems

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Communications in Theoretical Physics 2016年第12期65卷 615-620页

作者： naila Amir shahid Iqbal School of Electrical Engineering and Computer Sciences National University of Sciences and Technology School of Natural Sciences National University of Sciences and Technology

A generalized scheme for the construction of coherent states in the context of position-dependent effective mass systems has been presented. This formalism is based on the ladder operators and associated algebra of the system which are obtained using the concepts of supersymmetric quantum mechanics and the property of shape invariance. In order to exemplify the general results and to analyze the properties of the coherent states, several examples have been considered.

关键词： generalized coherent states supersymmetric quantum mechanics shape invariance ladder operators position-dependent effective mass systems non-linear oscillators Mandel parameter subPoissonian statistics

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Barut–Girardello Coherent States for Nonlinear Oscillator with Position-Dependent Mass

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Communications in Theoretical Physics 2016年第7期65卷 41-48页

Using ladder operators for the non-linear oscillator with position-dependent effective mass, realization of the dynamic group SU(1,1) is presented. Keeping in view the algebraic structure of the non-linear oscillator, coherent states are constructed using Barut–Girardello formalism and their basic properties are discussed. Furthermore, the statistical properties of these states are investigated by means of Mandel parameter and second order correlation function. Moreover,it is shown that in the harmonic limit, all the results obtained for the non-linear oscillator with spatially varying mass reduce to corresponding results of the linear oscillator with constant mass.

关键词： position-dependent mass nonlinear oscillator Schdinger factorization Ladder operators su(1 1) algebra Barut–Girardello coherent states sub-poissonian statistics

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Coherent States of Nonlinear Oscillators with Position-Dependent Mass:Temporal Stability and Fractional Revivals

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Communications in Theoretical Physics 2017年第8期68卷 181-190页

作者： naila Amir shahid Iqbal School of Electrical Engineering and Computer Sciences National University of Sciences and Technology Department of Physics School of Natural Sciences National University of Sciences and Technology

We develop generalized coherent states for a class of nonlinear oscillators with position-dependent effective mass in the context of the Gazeau–Klauder formalism and discuss some of their properties. In order to investigate the temporal evolution we first explore the statistical properties by means of weighting distribution and the Mandel parameter. It is found that the temporal evolution of the coherent states may exhibit the phenomena of quantum revivals and fractional revivals for a particular choice of position-dependent mass oscillator.

关键词： position-dependent effective mass nonlinear oscillators Gazeau–Klauder coherent states Mandel parameter sub-Poissonian statistics temporal stability quantum revivals fractional revivals

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Exact Solutions of Schrdinger Equation for the Position-Dependent Effective Mass Harmonic Oscillator

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Communications in Theoretical Physics 2014年第12期61卷 790-794页

作者： naila Amir shahid Iqbal School of Natural Sciences National University of Sciences and Technology

A one-dimensional harmonic oscillator with position-dependent effective mass is studied. We quantize the oscillator to obtain a quantum Hamiltonian, which is manifestly Hermitian in configuration space, and the exact solutions to the corresponding Schr¨odinger equation are obtained analytically in terms of modified Hermite *** is shown that the obtained solutions reduce to those of simple harmonic oscillator as the position dependence of the mass vanishes.

关键词： nonlinear harmonic oscillator position-dependent effective mass system Schr¨odinger equation modified Hermite polynomials

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CVIP-Net: A Convolutional Neural Network-Based Model for Forensic Radiology Image Classification

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Computers, Materials & Continua 2023年第1期74卷 1319-1332页

作者： Syeda naila Batool Ghulam Gilanie Department of Artificial Intelligence Faculty of ComputingThe Islamia University of BahawalpurBahawalpur63100Pakistan

Automated and autonomous decisions of image classification systems have essential applicability in this modern age ***-based decisions are commonly taken through explicit or auto-feature engineering of *** forensic radiology,auto decisions based on images significantly affect the automation of various *** study aims to assist forensic radiology in its biological profile estimation when only bones are left.A benchmarked dataset Radiology Society of North America(RSNA)has been used for research and ***,a locally developed dataset has also been used for research and experiments to cross-validate the results.A Convolutional Neural Network(CNN)-based model named computer vision and image processing-net(CVIP-Net)has been proposed to learn and classify image *** have also been performed on state-of-the-art pertained models,which are alex_net,inceptionv_3,google_net,Residual Network(resnet)_50,and Visual Geometry Group(VGG)-*** proved that the proposed CNN model is more accurate than other models when panoramic dental x-ray images are used to identify age and *** specially designed CNN-based achieved results in terms of standard evaluation measures including accuracy(98.90%),specificity(97.99%),sensitivity(99.34%),and Area under the Curve(AUC)-value(0.99)on the locally developed dataset to detect *** classification rates of the proposed model for gender estimation were 99.57%,97.67%,98.99%,and 0.98,achieved in terms of accuracy,specificity,sensitivity,and AUC-value,respectively,on the local *** classification rates of the proposed model for age estimation were 96.80%,96.80%,97.03%,and 0.99 achieved in terms of accuracy,specificity,sensitivity,and AUC-value,respectively,on the RSNA dataset.

关键词： Bones forensic analysis forensic radiology biology profile estimation age&gender estimation using dental x-ray images

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Phytochemical analysis and inhibitory effects of Calligonum polygonoides on pancreatic α-amylase and β-glucosidase enzymes

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Journal of Traditional Chinese Medicine 2022年第3期42卷 426-431页

作者： Mushtaq Ahmed naila Sher Nadia Mushtaq Rahmat Ali Khan Department of Biotechnology Faculty of Biological SciencesUniversity of Science&TechnologyBannu 28100KPK-Pakistan Department of Botany Faculty of Biological SciencesUniversity of Science&TechnologyBannu 28100KPKPakistan

OBJECTIVES:To estimate the existence of phytochemicals and then to determine the antidiabetic activity againstα-amylase andβ-glucosidase inhibition in ***:The study was carried out by following standard ***:Phytochemicals analysis indicated the presence of different *** total phenolic content was 6.055 mg GAE/g and the total flavonoid content was 5.706 mg RU/g in the plant *** total saponins,alkaloids,and tannins contents were(0.044%),(2.88%)and(2.862 nm)respectively.α-amylase inhibition activity of Calligonum polygonoides(CP)extract was 70%with IC50 of 610μg/mL and that ofβ-glucosidase inhibition activity was 65%with IC50 of 640μg/***:The findings reported for the first time the antidiabetes-promoting effects of an extract of CP in vitro,thus validating their promising anti-diabetes potential.

关键词： Calligonum polygonoides phytochemicals alpha-amylases beta-glucosidase

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