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To Grow or Not to Grow： FERONIA Has Her Say

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Molecular Plant 2014年第8期7卷 1261-1263页

作者： sha li Yan Zhang State Key Laboratory of Crop Biology College of Life Sciences Shandong Agricultural University Daizong St. 61 Tai’an Shandong China

Plant cells differ from their animal counterparts such that they are encased in a complex wall structure, crucial for cell growth through a dynamic equilibrium of rigidity and flexibility. Cell wall extensibility can be greatly influenced by apoplastic pH and reactive oxygen species （ROS）（Duan et al., 2014; Haruta et al., 2014）. Several recent works demonstrated that the receptor-like kinase （RLK） FERONIA （FER） is critical to fine-tune cell growth in a spatiotemporal and context-dependent manner by controlling apoplastic pH （Haruta et al., 2014） and ROS （Duan et al., 2010, 2014）, likely balancing wall rigidity for cell integrity and flexibility for cell expansion.

关键词： RECEPTOR-like kinases PLANT cells & tissues -- Growth PLANT growth ACTIVE oxygen PLANT gene expression

Constructing an Educational Knowledge Graph with Concepts linked to Wikipedia

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Journal of Computer Science & Technology 2021年第5期36卷 1200-1211页

作者： Fu-Rong Dang Jin-Tao Tang Kun-Yuan Pang Ting Wang sha-sha li Xiao li College of Computer Science and Technology National University of Defense TechnologyHunan 410073China Information Center National University of Defense TechnologyHunan 410073China

To use educational resources efficiently and dig out the nature of relations among MOOCs(massive open online courses),a knowledge graph was built for MOOCs on four major platforms:Coursera,EDX,XuetangX,and *** paper demonstrates the whole process of educational knowledge graph construction for *** this knowledge graph,the largest knowledge graph of MOOC resources at present,stores and represents five classes,11 kinds of relations and 52779 entities with their corresponding properties,amounting to more than 300000 ***,24188 concepts are extracted from text attributes of MOOCs and linked them directly with corresponding Wikipedia entries or the closest entries calculated semantically,which provides the normalized representation of knowledge and a more precise description for MOOCs far more than enriching words with explanatory ***,prerequisites discovered by direct extractions are viewed as an essential supplement to augment the connectivity in the knowledge *** knowledge graph could be considered as a collection of unified MOOC resources for learners and the abundant data for researchers on MOOC-related applications,such as prerequisites mining.

关键词： concept extraction educational resource knowledge graph massive open online course(MOOC) prerequisite

The Arabidopsis R-SNARE protein YKT61 is essential for gametophyte development

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Journal of Integrative Plant Biology 2021年第4期63卷 676-690页

作者： Ting Ma En li Lu-Shen li sha li Yan Zhang State Key laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai'an 271018China

Gametophyte development is a pre-requisite for plant reproduction and seed yield;therefore,studies of gametophyte development help us understand fundamental biological questions and have potential applications in *** biogenesis and dynamics of endomembrane compartments are critical for cell survival,and their regulatory mechanisms are just beginning to be ***,we report that the Arabidopsis thaliana SNARE(soluble N-ethylmaleimide sensitive factor attachment protein receptor)protein YKT61 is essential for both male and female *** using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-based genome editing,we demonstrated that male and female gametophytes carrying YKT61 loss-offunction alleles do not ***,loss of YKT61 function resulted in the arrest of male gametophytic development at pollen mitosis I and the degeneration of female gametophytes.A three-base-pair deletion in YKT61 in the ykt61-3 mutant resulted in a single-amino acid deletion in the longin domain of YKT61;the resulting mutant protein does not interact with multiple SNAREs and showed substantially reduced membrane association,suggesting that the N-terminal longin domain of YKT61 plays multiple roles in its *** study demonstrates that Arabidopsis YKT61 is essential for male and female gametogenesis and sets an example for functional characterization of essential genes with the combination of Cas9-mediated editing and expression from a Cas9-resistant transgene.

关键词： Arabidopsis ametogenesis embryo sac pollen pollen tube attraction SNARE

Hydrothermal Synthesis and Crystal Structure of a Cd^(2+) Supramolecular Coordination Complex{[Cd_2(HCAM)_2(H_2O)_4]·3.5H_2O}_n with a 3D Metal-organic Open Framework

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Chinese Journal of Structural Chemistry 2010年第7期29卷 1067-1071页

作者： ZHOU Qiu-Xiang li sha-sha WANG Meng BAI Xiao-Jie Langfang Teachers College Langfang 065000 China College of Chemical Engineering and Technology Hebei University of Technology Tianjin 300130 China

A three-dimensional coordination polymer {[Cd2（HCAM）2（H2O）4].***2O}n was synthesized by the hydrothermal reaction of H3CAM （H3CAM = 4-hydroxypyridine-2,6-dicerboxylic acid） and Cd（NO3）2·4H2O. The structure was characterized by elemental analysis, thermal gravimetric analysis, infrared spectroscopy and single-crystal X-ray diffraction. It crystallizes in triclinic, space group P1 with a = 9.4389（19）, b = 11.009（2）, c = 11.192（2） A, α = 87.87（3）, β= 74.63（3）, γ = 81.22（3）°, V= 1108.2（4） A3, Z = 2, Dc= 2.164 g/cm3,μ = 2.011 mm^-1, F（000） = 710, R = 0.0419 and wR = 0.1170. X-ray analysis reveals that the title compound adopts a one-dimensional staircase structure by carboxyl of H3CAM and water molecules, and it is further linked by hydrogen bonds to form a 3-D metal-organic open framework. The thermal gravimetric analysis displays that the framework exists stably below 300 ℃.

关键词： hydrothermal synthesis 3D coordination polymer crystal structure

Engineering of Ag@Pd/Al_(2)O_(3)with varied Pd-shell thickness:Dynamic evolution of ligand and strain effects on acetylene selective hydrogenation

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Chinese Journal of Chemical Engineering 2023年第12期64卷 139-148页

作者： Mingbo Yang Tianxing Yang Rui Ma sha li Yufei He Dianqing li State Key Laboratory of Chemical Engineering Beijing University of Chemical TechnologyBeijing 100029China Department of Medicine and Chemical Engineering Lianyungang Technical CollegeLianyungang 222006China Chemistry and Chemical Engineering Guangdong Laboratory Shantou 515031China Quzhou Institute for Innovation in Resource Chemical Engineering Quzhou 324000China Beijing Engineering Center for Hierarchical Catalysts Beijing University of Chemical TechnologyBeijing 100029China

Bimetallic nanoparticles exhibit a synergistic effect that critically depends on their surface composition,but such promotion mechanisms become vague with varying surface ***,alumina supported Ag@Pd core–shell and PdAg alloy structure with controlled size and surface compositions were prepared to demonstrate synergetic mechanisms,particularly,ligand and strain effects on activity and ethylene selectivity for acetylene *** performance evaluation indicates that Ag@Pd catalysts with well-controlled Pd-shell thickness can effectively lower apparent activation energy and improve ethylene *** activity increases from 0.019 to 0.062 s^(-1) with decreasing Pd-shell thickness under mild conditions,which is 3–6 times higher than their alloyed and monometallic *** characterizations and density functional theory are conducted to reveal such shell-thickness-dependent *** ligand effect arising from Ag alloying in the interface of Ag@Pd2ML observes the strongest binding of acetylene,but it diminished sharply and the strain effect gets more prevailing with increasing shell *** competition of ethylene desorption and deephydrogenation were also investigated to understand the selectivity governing factors,and the selectivity descriptor(0.5BE(C_(2)H_(4))–BE(H))was built to match the contribution of ligand and strain effect on the different surfaces of Pd-Ag bimetallic *** exploration of synergetic mechanisms among bimetallic NPs with varied structure and surface compositions in this work can help us to deepen the understanding catalyst structure–activity relationship and provide a feasible way to optimize the overall catalytic performance.

关键词： Catalyst Hydrogenation Nanostructure Shell-thickness-dependent performance ligand and strain effect

Piperine treating sciatica through regulating inflammation and MiR-520a/P65 pathway

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Chinese Journal of Natural Medicines 2021年第6期19卷 412-421页

作者： YU Jiu-Wang li sha BAO li-Dao WANG Lu Department of Pharmacy Affiliated Hospital of Inner Mongolia Medical UniversityHohhot 010059China Mongolia Medical School Inner Mongolia Medical UniversityHohhot 010110China

Although the etiology of sciatica remains uncertain,there is increasing evidence that the disease process of sciatica is associated with the levels of inflammatory ***,an alkaloid isolated from Piper nigrum,has previously been demonstrated to inhibit inflammation and analgesic *** purpose of this study is to verify the regulatory relationship between miR-520a and p65 and to explore how miR-520a/P65 affects the level of cytokines under the action of piperine,so as to play a therapeutic role in *** EliSA experiment,we confirmed that four inflammatory factors(IL-1β,TNF-α,IL-10,TGF-β1)can be used as evaluation indexes of *** differentially expressed miRNA was screened as miR-520a,by microarray technology,and the downstream target of miR-520a was P65 by ***-time fluorescence quantitative PCR confirmed that the expression of miR-520a was negatively correlated with pro-inflammatory cytokines,positively correlated with anti-inflammatory cytokines and negatively correlated with p65 expression at mRNA *** expression of p65 was positively correlated with pro-inflammatory cytokines and negatively correlated with anti-inflammatory cytokines at the protein level verified by EliSA and Western *** staining analysis showed that the nerve fibers were repaired by piprine,the vacuoles were significantly reduced,and the degree of nerve fiber damage was also *** analysis showed that the expression of p65 decreased after administration of ***-luciferase reporter gene assay confirmed that the luciferase signal decreased significantly after cotransfection of miR-520a mimics and p653'UTR recombinant *** sum up,in the rat model of non-compressed lumbar disc herniation,piperine plays a significant role in ***-520a can specifically and directly target P65,and piperine can promote the expression of miR-520a,then inhibit the expression of p65,down-regulate the pro-inflammatory

关键词： Piperine Sciatica miR-520a P65 Inflammatory factor

Factors influencing hysteresis characteristics of concrete dam deformation

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Water Science and Engineering 2017年第2期10卷 166-174页

作者： Jia-he Zhang Jian Wang li-sha Chai College of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 China lixi Barrage Irrigation Management Office of Guangzhou City Guangzhou 510890 China

Thermal deformation of a concrete dam changes periodically, and its variation lags behind the air temperature variation. The lag, known as the hysteresis time, is generally attributed to the low velocity of heat conduction in concrete, but this explanation is not entirely sufficient. In this paper, analytical solutions of displacement hysteresis time for a cantilever beam and an arch ring are derived. The influence of different factors on the displacement hysteresis time was examined. A finite element model was used to verify the reliability of the theoretical analytical solutions. The following conclusions are reached:(1) the hysteresis time of the mean temperature is longer than that of the linearly distributed temperature difference;(2) the dam type has a large impact on the displacement hysteresis time, and the hysteresis time of the horizontal displacement of an arch dam is longer than that of a gravity dam;(3) the reservoir water temperature variation lags behind of the air temperature variation, which intensifies the differences in the horizontal displacement hysteresis time between the gravity dam and the arch dam;(4) with a decrease in elevation, the horizontal displacement hysteresis time of a gravity dam tends to increase, whereas the horizontal displacement hysteresis time of an arch dam is likely to increase initially, and then decrease; and(5) along the width of the dam, the horizontal displacement hysteresis time of a gravity dam decreases as a whole, while the horizontal displacement hysteresis time of an arch dam is shorter near the center and longer near dam surfaces.

关键词： Concretedam Displacement Hysteresis Temperature Analytical solution

AMPK controls sucrose taste sensitization in Drosophila

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Journal of Genetics and Genomics 2023年第1期50卷 50-53页

作者： Yun Hu Zhi-Ying liu sha-sha li An-Qi li Qiao-Ping Wang Lab of Metabolism and Aging School of Pharmaceutical Sciences(Shenzhen)Shenzhen Campus of Sun Yat-sen UniversityShenzhenGuangdong 518107China

Sweet taste perception is critical for animals and *** an evolutionary perspective,sweet taste plays a key role in identifying carbohydrates *** taste perception in mammals is not fixed but is plasticity in the context of diets(Maliphol et al.,2013;Bartolotto,2015;Wise et al.,2016).However,so far it remains incomplete clear how diets affect sweet taste sensitivity.

关键词： Sweet taste incomplete

Effects of sludge characteristics on electrical resistance and energy consumption during electro-dewatering process

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Journal of Environmental Sciences 2021年第3期33卷 339-350页

作者： li sha Xiaoyan Yu Zhangxiang Wu Xingxin liu Shuting Zhang School of Environmental Science and Engineering Tianjin UniversityTianjin 300354China School of Environmental and Safety Engineering Taiyuan Institute of TechnologyTaiyuan 030008China

The increase of electrical resistance(ER)and energy consumption(EC)during the later stage of dewatering is a major problem hindering the development of electro-dewatering(EDW)*** the variations of sludge characteristics are significant during the EDW process,the relationships between sludge characteristics and ER and EC during EDW remain *** this study,the effects of moisture content(MC),thickness,pH,conductivity,zeta potential,temperature,and gas volume on the ER and EC during the EDW process were statistically investigated using correlation and multiple linear regression ***,the results showed that the ER of the sludge near the anode was primarily affected by pH,whereas the sludge near the cathode was primarily affected by the MC and ***,sludge temperature and conductivity were the most reliable indicators to predict the EC near the anode and cathode,*** results of this study provide theoretical guidance useful for solving the increase of ER and EC during the later stage of the EDW process.

关键词： Sludge Electro-dewatering Electrical resistance Energy consumption Multiple linear regression