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Potato Responds to Salt Stress by Increased Activity of Antioxidant Enzymes

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Journal of Integrative Plant Biology 2009年第12期51卷 1095-1103页

作者： Keyvan Aghaei Ali Akber Ehsanpour setsuko komatsu National Institute of Crop Science Tsukuba 305-8518 Japan Department of Biology Faculty of Sciences University of Isfahan Isfahan *** Iran

To understand the response of potato to salt stress, antioxidant enzyme activities and ion content were analyzed for a sensitive and a tolerant cultivar. Nodal cuttings of the tolerant cultivar, Kennebec, and the sensitive cultivar, Concord, were exposed to media without or with 30, 60, 90 or 120 mmol/L NaCI for 4 weeks. On exposure to NaCI, the length and fresh and dry weight of both shoots and roots of Concord showed greater decrease than those of Kennebec. The decrease in shoot growth was more severe than that of the root for both cultivars. The K^＋ content of shoots and roots of both cultivars was reduced in a dose-dependent manner by exposure to NaCl; the Na^＋ content increased. Activities of ascorbate peroxidase, catalase and glutathione reductase were increased in NaCl-exposed shoots of Kennebec; the corresponding activities in NaCI-exposed shoots of Concord were decreased. Roots of both cultivars showed similar changes in the activities of these enzymes on exposure to NaCI. These studies established that enzyme activities in Concord shoots are inversely related to the NaCI concentration, whereas those in Kennebec do not show a dose dependency, which is also the case for the roots of both cultivars. Our findings suggest that an increase in activity of antioxidant enzymes, such as ascorbate peroxidase, catalase and glutathione reductase, can contribute to salt tolerance in Kennebec, a salt resistant cultivar of potato.

关键词： antioxidant enzyme ion content potato salt resistant cultivar salt stress.

Functional Characterization of Gibberellin-Regulated Genes in Rice Using Microarray System

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Genomics, Proteomics & Bioinformatics 2006年第3期4卷 137-144页

作者： Asad Jan setsuko komatsu National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Crop Science Tsukuba 305-8518 Japan.

Gibberellin （GA） is collectively referred to a group of diterpenoid acids, some of which act as plant hormones and are essential for normal plant growth and development. DNA microarray technology has become the standard tool for the parallel quantification of large numbers of messenger RNA transcripts. The power of this approach has been demonstrated in dissecting plant physiology and development, and in unraveling the underlying cellular signaling pathways. To understand the molecular mechanism by which GA regulates the growth and development of plants, with reference to the monocot model plant-rice, it is essential to identify and analyze more genes and their products at the transcription and translation levels that are regulated by GA. With the availability of draft sequences of two major rice types, indica and japonica rice, it has become possible to analyze global expression profiles of genes on a genome scale. In this review, the progress made in finding new genes in rice leaf sheath using microarray system and their characterization is discussed. It is believed that the findings made in this regard have important implications for understanding the mechanism by which GA regulates the growth and development of rice.

关键词： gibberellin gene expression microarray rice

PROTEOME ANALYSIS OF RICE ROOT PROTEINS REGULATED BY GIBBERELLIN

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Genomics, Proteomics & Bioinformatics 2005年第3期3卷 132-142页

作者： setsuko komatsu HIROSATO KONISHI DEPARTMENT OF MOLECULAR GENETICS NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES TSUKUBA 305-8602 JAPAN

To gain an enhanced understanding of the mechanism by which gibberellins （GAs） regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA3, uniconazole, or abscisic acid （ABA）, and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA3, was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic in the GA-induced growth of roots. In among GA, aldolase, and root growth. pathway and may play an important role this review, we discuss the relationship among GA, aldolase, and root growth.

关键词： aldolase gibberellin proteome rice

Rice proteome analysis: a step toward functional analysis of the rice genome

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Rice proteome analysis: a step toward functional analysis of...

水稻中过表达OsCDPK和CRTintP1的蛋白质组学研究

首届全国蛋白质组学学术研讨会

作者： setsuko komatsu Department of Molecular Genetics National Institute of Agrobiological SciencesTsukubaJapan

The technique of proteome analysis using 2-DE has the power tomonitor global changes that occur in the protein complement of tissuesand subcellular *** this review,we describe construction ofthe rice proteome database,the cataloging of rice proteins,and thefunctional characterization of some of the proteins ***,proteins extracted from various tissues and organelles were separated by

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水稻中过表达OsCDPK和CRTintP1的蛋白质组学研究

The Quest for Missing Proteins in Rice

第二届热带亚热带植物资源的遗传多样性与基因发掘利用研讨...

作者：何道一 setsuko komatsu 淮北煤炭师范学院生物系国立农业生物学研究所分子遗传系

在植物的生长发育过程中,Ca2+信号传递系统有着广泛的作用,CDPKs和CRT是该途径中两个重要的组分,识别和传递植物细胞中Ca2+浓度的变化。多个CRT同源蛋白被分离纯化并被证明与植物逆境应答有关,但每个具体CRT的确切功能尚不清楚。作为一... 详细信息

在植物的生长发育过程中,Ca2+信号传递系统有着广泛的作用,CDPKs和CRT是该途径中两个重要的组分,识别和传递植物细胞中Ca2+浓度的变化。多个CRT同源蛋白被分离纯化并被证明与植物逆境应答有关,但每个具体CRT的确切功能尚不清楚。作为一个基因家族,CDPKs在拟南芥中有34个成员,在水稻中有27个成员,研究证明它们与多种细胞信号传导有关,但每个成员的功能还不明确。为了解析OsCDPK13和CRT在水稻抗低温逆境中的作用与机制,我们用OsCDPK13和 CRTintP1分别转化抗寒性不同的水稻品种,并用蛋白质组学方法研究组成型表达OsCDPK13和 CRTintP1对水稻中蛋白质表达和蛋白质修饰的影响。 Western blot结果表明,不同抗寒能力的水稻品种其OsCDPK13、CRT和CRTintP1表达水平不同。当OsCDPK13和CRTintP1组成型表达时,所有品种抗寒能力提高。这表明OsCDPK13、CRT和 CRTintP1在水稻抗低温逆境中起一定作用。激酶凝胶分析表明,在组成型表达OsCDPK13转基因植株中,蛋白质激酶的活性显著提高,而在转反义OsCDPK13的转基因植株中蛋白质激酶活性明显降低。同时发现,在组成型表达OsCDPK13的植株中多种蛋白质磷酸化水平发生变化。2-DE分析表明, 在组成型表达OsCDPK13的植株中12个细胞质中的蛋白质表达水平发生变化。

关键词：水稻抗寒 OsCDPK13 Ca2+信号蛋白质组

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Molecular Plant 2019年第1期12卷 4-6页

作者： Mohsen Rahiminejad Mohammad Taheri Ledari Mehdi Mirzaei Zahra Ghorbanzadeh Kaveh.Kavousi Mohammad Reza Ghaffari Paul A Haynes setsuko komatsu Ghasem Hosseini Salekdeh Department of Systems Biology Agricultural Biotechnology Research Institute of Iran (ABRII)Agricultural Research Education and Extension Organization (AREO)KarajIran Department of Biotechnology University of TehranTehranIran Institute of Biochemistry and Biophysics (IBB) University of TehranTehran Iran Department of Molecular Sciences Macquarie UniversitySydneyAustralia Australian Proteome Analysis Facility Macquarie UniversitySydneyAustralia Faculty of Life and Environmental Sciences University of TsukubaTsukuba Japan

Rice (Oryza sativa)is the primary staple food source for more than half of the world's *** are active macromolecules and central to understanding biological molecular processes. Hence,it is necessary to study all protein forms of plant genes so that their biological functions and roles in regulating important traits such as yield,quality,and responses to environmental stresses can be determined.

关键词： Rice food

Experiences of patients with HIV/AIDS receiving mid- and long-term care in Japan:A qualitative study

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International Journal of Nursing Sciences 2017年第2期4卷 99-104页

作者： Yoko Imazu Nao Matsuyama Sanae Takebayashi Mizue Mori setsuko Watabe Tokyo Medical and Dental University TokyoJapan Yokohama City University Hospital YokohamaJapan Yokohama City University YokohamaJapan Department of Nursing Graduate School of MedicineYokohama City University Yokohama236-0004Japan

Purpose: In the era of antiretroviral treatment (ART), treatment of HIV has become more manageable, and most patients with HIV benefit from long-term therapy in Japan. The aim of this qualitative study was to examine the experiences of patients with HIV/AIDS receiving mid-and long-term care in Japan. Methods: A qualitative study was performed using semi-structured interviews at an outpatient clinic of a university hospital in the Greater Tokyo area. A total of 31 Japanese patients with HIV who had been receiving treatment for at least a year were interviewed in Japanese. Data from these interviews were analyzed by'content analysis' (Krippendorff, 1980). Results: The data were organized into the following seven themes:"feelings toward diagnosis of HIV infection";"perceptions and behavior after diagnosis of HIV";"attitudes toward HIV therapy";"fear and hope for the future";"feelings toward professional support";"life changes after HIV therapy";and"struggles in relationships with others". Conclusions: Some participants accepted themselves as HIV-positive, some did not when they were diagnosis. This difference of reaction had effect on attitudes toward HIV therapy. But all participants continued mid- and long-treatments owing to feeling well and receiving support from professionals. Additionally, it found that patients were felt stigma of HIV-positive similarly to another Asian countries. Therefore, we should provide the entire Japanese society with knowledge about HIV/AIDS and be early and constant intervention by professional teams after being infected.

关键词： patients qualitative continued

Predictive model of risk factors of High Flow Nasal Cannula using machine learning in COVID-19

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Infectious Disease Modelling 2022年第3期7卷 526-534页

作者： Nobuaki Matsunaga Keisuke Kamata Yusuke Asai Shinya Tsuzuki Yasuaki Sakamoto Shinpei Ijichi Takayuki Akiyama a Jiefu Yu Gen Yamada Mari Terada setsuko Suzuki Kumiko Suzuki Sho Saito Kayoko Hayakawa Norio Ohmagari AMR Clinical Reference Center National Center for Global Health and MedicineTokyoJapan DataRobot Inc. TokyoJapan Disease Control and Prevention Center National Center for Global Health and MedicineTokyoJapan Biostatistics Division Center for Research Administration and SupportNational Cancer CenterTokyoJapan

With the rapid increase in the number of COVID-19 patients in Japan,the number of patients receiving oxygen at home has also increased rapidly,and some of these patients have *** efficient approach to identify high-risk patients with slowly progressing and rapidly worsening COVID-19,and to avoid missing the timing of therapeutic intervention will improve patient prognosis and prevent medical *** admitted to medical institutions in Japan from November 14,2020 to April 11,2021 and registered in the COVID-19 Registry Japan were *** factors for patients with High Flow Nasal Cannula invasive respiratory management or higher were comprehensively explored using machine ***-specific cohorts were created,and severity prediction was performed for the patient surge *** were able to obtain a model that was able to predict severe disease with a sensitivity of 57%when the specificity was set at 90%for those aged 40e59 years,and with a specificity of 50%and 43%when the sensitivity was set at 90%for those aged 60e79 years and 80 years and older,*** were able to identify lactate dehydrogenase level(LDH)as an important factor in predicting the severity of illness in all age *** machine learning,we were able to identify risk factors with high accuracy,and predict the severity of the *** plan to develop a tool that will be useful in determining the indications for hospitalisation for patients undergoing home care and early hospitalisation.

关键词： COVID-19 Machine learning Severity Risk prediction Japan

Vowel Reduction in Japanese

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南开语言学刊 2009年第2期 14-20页

作者： Shirai setsuko MingDao University

This paper reports the result that vowel reduction occurs in Japanese and vowel reduction is the part of the language *** with English,the effect of the vowel reduction in Japanese is relatively weak might because of the absence of stress in *** spectral vowel reduction occurs in Japanese,various types of researches would be possible.

关键词： vowel reduction Japanese experimental analysis