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甘薯块根花青素生物合成的转录组学和代谢组学分析

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甘薯块根花青素生物合成的转录组学和代谢组学分析

作者： Hanna Amoanimaa-dede 广东海洋大学

学位级别：硕士

花青素属于黄酮类化合物,具有多种生理功能。紫甘薯具有含有丰富的花青素,是低分子量抗氧化剂的来源,对健康有益。因此,随着人们健康意识和食品安全意识的提高,紫肉甘薯作为一种新兴的保健食品备受青睐。因此,了解花青素的生物合成和积... 详细信息

花青素属于黄酮类化合物,具有多种生理功能。紫甘薯具有含有丰富的花青素,是低分子量抗氧化剂的来源,对健康有益。因此,随着人们健康意识和食品安全意识的提高,紫肉甘薯作为一种新兴的保健食品备受青睐。因此,了解花青素的生物合成和积累机制,对于培育更多富含花青素的甘薯品种具有重要意义。本课题结合转录组和代谢组分析,研究了不同薯肉色甘薯的代谢物分布的基因表达模式,探讨了花青素的合成和积累是调控模式。该项研究成果具有潜在的应用价值,因为它提供了花青素生物合成和积累的内部机制,将进一步促进甘薯花青素含量优良品种的分子育种。为了解花青素生物合成和积累的分子机制,对肉色不同的甘薯品种,进行了全转录组和代谢组学分析;利用NCBI-Blastp筛选花青素相关基因,并对其功能进行注释。此外,进行q RT-PCR分析以验证随机选择的10个基因的表达模式。结果表明:广薯87和紫罗兰的转录组测序数据显示,从61335186个高质量的阅读片段中组装出24388个单基因。4469个是差异表达基因（DEGs）,其中2532个上调,1937个下调。在非冗余蛋白质数据库中,共有3602个DEGs（80.6%）被注释有功能。GO分析将DEGs分为细胞成分、生物过程和分子功能等48个GO术语。185-P和185-W转录组测序数据中,共有239415012个高质量的读取被组装到27889个单基因中。共检测到264个差异表达基因（DEGs）,其中100个上调基因和164个下调基因,其中255个（96.6%）在公共数据库中被注释为假定功能。GO分类将DEGs分为36个GO术语,分别属于细胞成分、生物过程和分子功能类别。根据GO分析,生物过程和分子功能类别下的19个术语与两个DEG组（GS87 vs ZLL和185-P vs 185-W）中的花青素生物合成有关。我们的结果表明,黄酮类和苯丙烷类的生物合成途径是花青素生物合成的主要信号途径。除了F3′5′H外,几乎所有与花青素生物合成相关的途径基因包括ANS、DFR、CHS、F3H、CHI、F3H和3GT都被鉴定出来,这是由于该基因在甘薯属植物中丢失所致。NCBI-Blastp序列分析表明,这些基因与其它植物如拟南芥、矮牵牛、水稻和一些甘薯属植物如***,*** and ***。此外,MBW复合物的b HLH和MYB-TFs被鉴定为调节花青素生物合成的主要TFs。值得注意的是,几乎所有与花青素生物合成相关的TF和结构基因都显著上调。此外,还进行了q RT-PCR分析,以验证随机选择的10个基因的表达模式。结果与转录组测序数据一致,证实了测序数据可靠性较高。所有四个花青素相关基因在紫色品种中均显著上调,表明它们在花青素生物合成中的作用。从代谢组中,共鉴定出122种代谢物,其中53种代谢物（6种上调,47种下调）在两个品种（GS87和ZLL）之间表现出差异表达模式。主成分分析和聚类分析表明,不同品种间的代谢特征不同,代谢产物主要富集黄酮和花青素类物质。

关键词：甘薯花青素转录组代谢组基因表达

Determination of Indicator Plants for Boron in the Kirka(Eskisehir/Turkey)Boron Deposit Area

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International Journal of Geosciences 2014年第1期5卷 77-84页

作者： Zeynep Ozdemir semiha Zorlu Mustafa Akyildiz Fulya Yücesoy Eryilmaz Department of Geology Engineering Mersin UniversityMersinTurkey Department of Geology Engineering Cukurova UniversityAdanaTurkey

The amount of elements in plants is important for biogeochemical explorations. Some plants which were accumulated extremely elements, are called indicator plants. In this study, the 11 plant species and soil samples were collected boron deposits area in Kirka (Eskisehir). Boron is determinated in plants (leaves and twigs) and soil samples. Additionally, statistical relations were established between the B values of plant and soil samples and three plants were only found. The boron in twigs of Puccinellia intermedia (n = 17, r = 0.7274), twigs of Genista aucheri (n = 26, r = 0.8581 and leaves of Pinus nigra (n = 16, r = 0.6805) species are found 119, 81 and 115 mg/kg respectively. This species was reflected boron in soil and their used indicator plant for boron. The indicator plants could be successfully used for biogeochemical prospecting and environmental monitoring.

关键词： Biogeochemistry Boron Indicator Plant Kirka(Eskisehir/Turkey)

剥脱综合征患者结膜印迹细胞学的检查特征（英文）

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国际眼科杂志 2015年第6期15卷 953-958页

作者： Ayse Gül Kocak Altintas semiha Has Eranil Olcay Kandemir Saglik. Bakanligi Ulucanlar 眼科医院土耳其安卡拉06080 Saglik. Bakanligi Yenimahalle医院土耳其安卡拉06080 Saglik. Bakanligi Onkoloji医院土耳其安卡拉06080

目的:研究剥脱综合征对结膜细胞和泪液功能的影响。方法:研究共纳入剥脱综合征患者60例86眼作为试验组,年龄相仿正常人55例89眼作为对照组。所有115例175眼均接受Schirmer泪试验及泪膜破裂时间试验,并对上方和鼻下方睑裂区球结膜进行印... 详细信息

目的:研究剥脱综合征对结膜细胞和泪液功能的影响。方法:研究共纳入剥脱综合征患者60例86眼作为试验组,年龄相仿正常人55例89眼作为对照组。所有115例175眼均接受Schirmer泪试验及泪膜破裂时间试验,并对上方和鼻下方睑裂区球结膜进行印迹细胞学检查。印迹细胞学检查结果根据Nelson方法分级。对TBUT,Schirmer泪试验及印迹细胞学结果进行相关性分析。结果:剥脱综合征患者的Schirmer试验、TBUT均较正常组显著降低,结膜细胞也有显著改变。剥脱综合征患者的印迹细胞学分级中位数为3级,而正常人组为0级。在试验组中发现45例样本出现了两种以上分级共存的现象,而对照组仅发现3例。此外,实验组中的45例样本结膜上皮细胞和杯状细胞分级出现明显差异,且均表现出杯状细胞的破坏甚于上皮细胞,而对照组未发现此种现象。结论:剥脱综合征患者的结膜细胞受到破坏,泪液分泌受到影响,极易造成眼表疾病的出现。

关键词：印迹细胞学剥脱综合征泪膜破裂时间 Schirmer泪试验

Alanine aminotransferase is an inadequate surrogate marker for detecting lamivudine resistance

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World Journal of Gastroenterology 2010年第37期16卷 4691-4696页

作者： Lee Guan Lim Myat Oo Aung Bee Leng Seet Cindy Tan Yock Young Dan Yin Mei Lee dede Selamat Sutedja Mark Fernandes Guan Huei Lee Evelyn Koay Seng Gee Lim Department of Gastroenterology and Hepatology National University Health System Yong Yoo Lin School of Medicine National University of Singapore Department of Medicine National University Health System Yong Yoo Lin School of Medicine National University of Singapore Molecular Diagnostic Centre National University Health System Yong Yoo Lin School of Medicine National University of Singapore Investigative Medicine Unit National University Health System Yong Yoo Lin School of Medicine National University of Singapore

AIM: To investigate the accuracy of serum alanine aminotransferase (ALT) in diagnosing lamivudine resistance and factors that contributed to abnormal serum ***: This was a retrospective study of chronic hepatitis B patients on lamivudine therapy who were followed for 3-mo with liver function tests and hepatitis B virus (HBV) DNA measurement. Lamivudine resistance was defined as HBV DNA ≥ 1 log from nadir on at least 2 occasions, confirmed by genotyping. Serum ALT levels in patients with lamivudine resistance were compared to serum ALT levels in those without lamivudine resistance. RESULTS: There were 111 patients with and 117 without lamivudine resistance. The area under the receiver operating characteristic of serum ALT to diagnose lamivudine resistance was 0.645 ± 0.037. Serum ALT > 42.5 U/L gave the best diagnostic accuracy with sensitivity = 61%, specificity = 60%, positive predictive value = 60%, negative predictive value = 61%, positive likelihood ratio = 1.53 and negative likelihood ratio = 0.65 for predicting lamivudine resistance, missing 39% of resistant patients. Using other serum ALT cutoffs, diagnostic accuracy was lower. By multivariate analysis, baseline abnormal serum ALT was associated with abnormal ALT during resistance (OR = 5.98, P = 0.003), and males were associated with serum ALT flares during resistance (OR = 8.9, P = 0.016). CONCLUSION: Serum ALT is inadequate for diagnosing lamivudine resistance and has implications where viral resistance testing is suboptimal and for reimbursement of rescue therapy.

关键词： Antiviral therapy Chronic hepatitis B Sensitivity Specificity YMDD mutants

Swelling Performance Studies of Acrylamide/Potassium 3-Sulfopropyl Methacrylate/Sodium Alginate/Bentonite Biohybrid Sorbent Hydrogels in Binary Mixtures of Water-Solvent

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Journal of Encapsulation and Adsorption Sciences 2019年第1期9卷 35-61页

作者： Erdener Karadag Z.Deniz Kasim Oztürk Ö mer Baris Uzüm semiha Kundakci Chemistry Department Fen-Edebiyat FacultyAdnan Menderes UniversityAydinTurkey

In this study, it was to investigate the swelling performance of novel biohybrid composite hydrogel sorbents containing acrylamide/potassium 3-sulfopropyl methacrylate/sodium alginate/bentonite in water and binary mixtures of water-solvent. Novel hydrogels were synthesized with free radical solution polymerization by using ammonium persulfate/N,N,N’,N’-tetramethylethylenediamine as redox initiating pair in presence of poly(ethylene glycol) diacrylate as crosslinker. Swelling experiments were performed in water and binary mixtures of water-solvent (acetone, methanol and tetrahydrofuran) at 25°C, gravimetrically. Some swelling and diffusion properties of the hydrogels were calculated and they were discussed for the biohybrid/hybrid hydrogel systems prepared under various formulations. It has been seen the lower equilibrium percentage swelling ratio values (62% - 124%) in all solvent compositions in comparison with the equilibrium percentage swelling ratio values in water (718% - 2055%). Consequently, the hydrogel systems developed in this study could serve as a potential device for water and water-solvent binary mixtures.

关键词： Acrylamide/Potassium 3-Sulfopropyl Methacrylate Biohybrid Hydrogel Swelling Sodium Alginate Bentonite Water-Solvent Binary Mixtures

Merging GIS and Machine Learning Techniques: A Paper Review

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Journal of Geoscience and Environment Protection 2022年第9期10卷 61-83页

作者： Chikodinaka Vanessa Ekeanyanwu Inioluwa Feranmi Obisakin Precious Aduwenye Nathaniel dede-Bamfo Department of Geography and Environmental Studies Texas State University San Marcos TX USA Ingram School of Engineering Texas State University San Marcos TX USA

GIS (Geographic Information Systems) data showcase locations of earth observations or features, their associated attributes and spatial relationships that exist between such observations. Analysis of GIS data varies widely and may include some modeling and predictions which are usually computing-intensive and complicated, especially, when large datasets are involved. With advancement in computing technologies, techniques such as Machine learning (ML) are being suggested as a potential game changer in the analysis of GIS data because of their comparative speed, accuracy, automation, and repeatability. Perhaps, the greatest benefit of using both GIS and ML is the ability to transfer results from one database to another. GIS and ML tools have been used extensively in medicine, urban development, and environmental modeling such as landslide susceptibility prediction (LSP). There is also the problem of data loss during conversion between GIS systems in medicine, while in geotechnical areas such as erosion and flood prediction, lack of data and variability in soil has limited the use of GIS and ML techniques. This paper gives an overview of the current ML methods that have been incorporated into the spatial analysis of data obtained from GIS tools for LSP, health, and urban development. The use of Supervised Machine Learning (SML) algorithms such as decision trees, SVM, KNN, and perceptron including Unsupervised Machine Learning algorithms such as k-means, elbow algorithms, and hierarchal algorithm have been discussed. Their benefits, as well as their shortcomings as studied by several researchers have been elucidated in this review. Finally, this review also discusses future optimization techniques.

关键词： GIS Machine Learning Landslide Susceptibility Random Forest Urban Development Flood Prediction Health GeoAI

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氯化高铁血红素衍生物催化鲁米诺发光的研究

分析化学 1999年第4期27卷 412-414页

作者：林的的杨学强黄英孺中山大学化学系中山大学化学系广州510275

用氯化血红素为原料合成氯化高铁血红素衍生物DFH（Chlorodeuteroferrihaem）.系统考察了DFH催化鲁米诺与H;O;反应的发光性能,并和过氧化物酶的催化性能作了比较.所得结果为DFH代替过氧化物酶应用于化学发光免疫分析提供了直接依据.

关键词：鲁米诺化学发光过氧化物酶

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磁热噪音下纳米铁磁线磁畴壁脱钉行为

科学通报 2013年第28期58卷 2927-2933页

作者：马晓萍沈帝虎李尚赫 DJUHANA dede 朴红光金东炫三峡大学理学院宜昌443002 Department of Physics Chungbuk National UniversityCheongju 361-763Republic of Korea 清华大学材料学院北京100084

为了考察由热起伏引起的磁性噪音(磁热噪音)对纳米铁磁线磁畴壁动力学的影响,通过微磁学模拟研究了在不同温度磁热噪音下纳米铁磁线磁畴壁脱钉(depinning)行为.为了分析磁畴壁的钉扎和脱钉行为,在纳米铁磁线上制作了一个纳米凹槽使磁畴... 详细信息

为了考察由热起伏引起的磁性噪音(磁热噪音)对纳米铁磁线磁畴壁动力学的影响,通过微磁学模拟研究了在不同温度磁热噪音下纳米铁磁线磁畴壁脱钉(depinning)行为.为了分析磁畴壁的钉扎和脱钉行为,在纳米铁磁线上制作了一个纳米凹槽使磁畴壁牢牢地钉扎(pinning)在凹槽中.在外磁场驱动下,利用纳米铁磁线的纳米凹槽考察了磁畴壁的钉扎和脱钉行为.结果表明,在无磁热噪音条件(T=0 K)下,磁畴壁在不同纳米凹槽中受到"U"或"W"字形状能量势阱的约束,影响了磁畴壁的钉扎状态和脱钉行为.在磁热噪音条件(T≠0 K)下,纳米铁磁线磁畴壁虽然保持着自旋结构上的特征和动力学上的行为特性,但是随着温度升高,磁热噪音对纳米凹槽能量势阱的影响明显增强,不仅纳米凹槽的能量势阱形状发生变化,而且磁畴壁在纳米凹槽中的脱钉磁场强度也发生改变.这意味着在磁热噪音下利用纳米线的几何形状不易有效调控磁畴壁的动力学行为.

关键词：纳米铁磁线磁畴壁运动磁热噪音微磁学模拟

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晶体管在感应加热设备上取代电子管和晶闸管

电工技术交流 1994年第1期 55-60页

作者： dede,EJ 姜寄复