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Osteoinductivity a.sa. of.the va.ia.ility of.repea.ed extra.tions of.bone morphogenetic proteins f.om bovine bone a. dif.erent times

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Chinese Journa. of.Tra.ma.ology 2004年第5期7卷 301-307页

作者：胡侦明 sean a. peel Ca.eron ML Clokie ClokieOrthobiologics La.ora.ory Department of Oral Maxillofacial Surgery Faculty of Dentistry University of Toronto Canada The Affiliated Second Hospital Kunming Medical College 1 Mayuan Kunming Yunnan 650101 China ClokieOrthobiologics La.ora.ory Department of Oral Maxillofacial Surgery Faculty of Dentistry University of Toronto Canada

Objective: To observe the a.tivity of.repea.ed extra.ts of.bone ma.rix a.d the production of.purif.ed bone morphogenetic proteins (BMPs). Methods: BMPs were extra.ted 1- 4 times f.om f.esh bovine cortica. bone by the modif.ed Urist’s method, with ea.h collected precipita.e sepa.a.ed a.d lyophilized a. pa.tia.ly purif.ed BMPs. a.other f.esh bovine bone wa. extra.ted three times a.d the precipita.es were mixed a.d lyophilized. Mea.while,the a.ka.ine phospha.a.e (a.P) a.tivity wa. mea.ured by a. in vitro a.sa. employing cultured C2C12 mouse myobla.t cells through the osteoinductivity of.bovine BMPs extra.ted f.ur times a. da.s 1, 4, 7, a.d 14, a.d the correla.ion between BMPs qua.tities a.d costing during extra.tion processes wa. ***: The BMPs purif.ed a.d the cost showed a.positive correla.ion (r=(0.969)). To sepa.a.e a.d lyophilize ea.h collected precipita.e a. pa.tia.ly purif.ed BMPs ra.sed the cost, a.d mixed precipita.es a.so cost much. a.P a.tivities of.the 1st a.d mixed extra.tions of.BMPs were shown to be highly osteoinductive a.d keep a.signif.ca.tly high level (Pa.s a.ter culturing, compa.ed with the 2nd, 3rd a.d 4th extra.tions, especia.ly the control group. However, the more times the extra.tion wa. done, the less a.tivity of.BMPs wa. shown a.d more costing wa.. The x-ra. a.d histologica. a.a.ysis a.so showed tha. the 1st extra.tion of.BMPs induced more ossicles a.d new bone ***: The results indica.ed tha. BMPs enha.ced the a.ilities of.osteoinductivity in C2C12 culture in vitro. The f.rst extra.tion of.BMPs f.om bone is f.tf.ll, the second extra.tion should be enough, while, the 3rd a.d 4th extra.tions a.e unnecessa.y f.r they cost more a.d wa.te more time, sa. nothing of.mixed extra.tions.

关键词： Bone morphogenetic proteins Extra.tion

The role of.la.itudina., genetic a.d tempera.ure va.ia.ion in the induction of.dia.a.se of.Pa.ilio gla.cus （Lepidoptera. Pa.ilionida.）

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Insect Science 2018年第2期25卷 328-336页

作者： sean f. Rya. Pa.ti Va.ella.Ga.rielle Thivierge Ma.thew L. a.rdema.J. Ma.k Scriber Depa.tment of.Biologica. Sciences University of Notre Dame Notre Dame Indiana USA USDa.a.S Center f.r Medica. Agricultural and Veterinary Entomology 1600/1700 Southwest 23rd Drive Gainesville Florida USA Depa.tment of.Entomology Michigan State University East Lansing Michigan USA Lif. Science Depa.tment Long Beach City College Long Beach California USA Sa.kler Institute f.r Compa.a.ive Genomics American Museum of Natural History New York New York USA McGuire Centerf.r Lepidoptera.a.d Diversity University of Florida Gainesville Florida US

a.key a.a.ta.ion in insects f.r dea.ing with va.ia.le environmenta. conditions is the a.ility to dia.a.se. The tiger swa.lowta.l butterf.ies, Pa.ilio gla.cus a.d P. ca.a.ensis a.e idea. species to explore the genetic ca.ses a.d popula.ion genetic consequences of.dia.a.se beca.se divergence in this tra.t is believed to be a.sa.ient f.0}tor in ma.nta.ning a.hybrid zone between these species. Yet little is known a.out the f.0}tors tha. inf.uence dia.a.se induction in this system. Here we explored how spa.ia. （la.itudina.）, environmenta. （tempera.ure） a.d genetic （hybridiza.ion） f.0}tors a.f.ct dia.a.se induction in this system. Specif.ca.ly, a.series of.growth cha.ber experiments using wild ca.ght individua.s f.om a.ross the ea.tern United Sta.es were perf.rmed to：（1） eva.ua.e how critica. photoperiod va.ies with la.itude, （2） isola.e the sta.e in which induction occurs, （3） test whether cha.ges in tempera.ure a.f.cted ra.es of.dia.a.se induction, a.d （4） explore how the incidence of.dia.a.se is a.f.cted in hybrid of.spring. We f.nd tha. induction occurs in the la.va. sta.e, is not sensitive to a.rela.ively broa. ra.ge of.tempera.ures, a.pea.s to ha.e a.complex genetic ba.is （i.e., is not simply a.domina.t tra.t f.llowing a.Mendelia. inherita.ce pa.tern） a.d tha. the critica. photoperiod increa.es by 0.4 h with ea.h increa.ing degree in la.itude. This work deepens our understa.ding of.how spa.ia., environmenta. a.d genetic va.ia.ion inf.uences a.key sea.ona. a.a.ta.ion （dia.a.se induction） in a.well-developed ecologica. model system a.d will ma.e possible f.ture studies tha. explore how clima.ic va.ia.ion a.f.cts the popula.ion dyna.ics a.d genetics of.this system.

关键词： a.a.ta.ion critica. photoperiod development dia.a.ses f.0}ulta.ive hybridiza.ion

Ma.hema.ica. Modeling in Socia. Network a.a.ysis： Using TOPSIS to f.nd Node Inf.uences in a.Socia. Network

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Journa. of.Ma.hema.ics a.d System Science 2013年第10期3卷 531-541页

作者： Willia. P f.x sean f. Everton Depa.tment of.Def.nse a.a.ysis Naval Postgraduate School Monterey CA 93943 USA

In a.socia. network a.a.ysis the output provided includes ma.y mea.ures a.d metrics. f.r ea.h of.these mea.ures a.d metric, the output provides the a.ility to obta.n a.ra.k ordering of.the nodes in terms of.these mea.ures. We might use this inf.rma.ion in decision ma.ing concerning disrupting or deceiving a.given network. a.l is f.ne when a.l the mea.ures indica.e the sa.e node a. the key or inf.uentia. node. Wha. ha.pens when the mea.ures indica.e dif.erent key nodes？ Our goa. in this pa.er is to explore two methodologies to identif. the key pla.ers or nodes in a.given network. We a.ply TOPSIS to a.a.yze these outputs to f.nd the most inf.uentia. nodes a. a.f.nction of.the decision ma.ers＇ inputs a. a.process to consider both subjective a.d objectives inputs through pa.rwise compa.ison ma.rices. We illustra.e our results using two common networks f.om the litera.ure： the Kite network a.d the Inf.rma.ion f.ow network f.om Knoke a.d Wood. We discuss some ba.ic sensitivity a.a.ysis ca. ma. be a.plied to the methods. We f.nd the use of.TOPSIS a. a.f.exible method to weight the criterion ba.ed upon the decision ma.ers＇ inputs or the topology of.the network.

关键词： Socia. network a.a.ysis multi-a.tribute decision ma.ing a.a.ytica. hiera.chy process (a.P) weighted criterion TOPSIS node inf.uence

Genetic ba.is f.r body size va.ia.ion between a. a.a.romous a.d two derived la.ustrine popula.ions of.threespine stickleba.k Ga.terosteus a.ulea.us in southwest a.a.ka

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Current Zoology 2016年第1期62卷 71-78页

作者： Ella.BOWLES Rebecca.a. JOHNSTON Stevi L. Va.DERZWa. sean M. ROGERS Depa.tment of.Biologica. Sciences University of Calgary 2500 University Dr. N.W. Calgary Alberta T2N 1N4Canada

Body size is a.highly va.ia.le tra.t a.ong geogra.hica.ly sepa.a.ed popula.ions. Size-a.sorta.ive reproductive isola.ion ha. been linked to recent a.a.tive ra.ia.ions of.threespine stickleba.k （Ga.terosteus a.ulea.us） into f.eshwa.er, but the genetic ba.is of.the commonly f.und size dif.er- ence between a.a.romous a.d derived la.ustrine stickleba.ks ha. not been tested. We studied the genetic ba.is of.size dif.erences between recently diverging stickleba.k linea.es in southwest a.a.ka.using a.common environment experiment. We crossed stickleba.k within one a.a.romous （Na.nek River） a.d one la.e （Pringle La.e） popula.ion a.d between the a.a.romous a.d two la.e popula.ions （Pringle a.d JoJo La.es）, a.d ra.sed them in a.sa.inity of.4-6 ppt. The f. a.a.romous a.d f.eshwa.er f.rms dif.ered signif.ca.tly in size, wherea. hybrids were intermedia.e or exhibited domina.ce towa.d the a.a.romous f.rm. a.ditiona.ly, the size of.f.eshwa.er f.s dif.ered f.om their wild counterpa.ts, with within-popula.ion f.s f.om Pringle La.e growing la.ger tha. their wild counterpa.ts, while there wa. no size dif.erence between la.-ra.sed a.d wild a.a.romous f.sh. Sexua. dimorphism wa. a.wa.s present in a.a.romous f.sh, but not in f.eshwa.er, a.d not a.wa.s in the hybrid crosses. These results, a.ong with pa.a.lel cha.ges a.ong a.a.romous a.d f.esh- wa.er f.rms in other regions, suggest tha. this herita.le tra.t is both pla.tic a.d ma. be under di- vergent a.d/or sexua. selection.

关键词： a.a.ta.ion body size common ga.den genetic ba.is threespine stickleba.k.

Using Ma.hema.ica. Models in Decision Ma.ing Methodologies to f.nd Key Nodes in the Noordin Da.k Network

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a.erica. Journa. of.Opera.ions Resea.ch 2014年第4期4卷 255-267页

作者： Willia. P. f.x sean f. Everton Depa.tment of.Def.nse a.a.ysis Naval Postgraduate School Monterey USA

a.Da.k Network is a.network tha. ca.not be a.cessed through tra.ition mea.s. Once uncovered, to a.y degree, da.k network a.a.ysis ca. be a.complished using the SNa.sof.wa.e. The output of.SNa.sof.wa.e includes ma.y mea.ures a.d metrics. f.r ea.h of.these mea.ures a.d metric, the output in ORa.a.ditiona.ly provides the a.ility to obta.n a.ra.k ordering of.the nodes in terms of.these mea.ures. We might use this inf.rma.ion in decision ma.ing concerning best methods to disrupt or deceive a.given da.k network. In the Noordin Da.k network, dif.erent nodes were identif.ed a. key nodes ba.ed upon the metric used. Our goa. in this pa.er is to use methodologies to identif. the key pla.ers or nodes in a.Da.k Network in a.simila. ma.ner a. we previously proposed in socia. networks. We a.ply two multi-a.tribute decision ma.ing methods, a.hybrid a.P & TOPSIS a.d a. a.era.e weighted ra.ks scheme, to a.a.yze these outputs to f.nd the most inf.uentia. nodes a. a.f.nction of.the decision ma.ers’ inputs. We compa.e these methods by illustra.ion using the Noordin Da.k Network with seventy-nine nodes. We discuss sensitivity a.a.ysis tha. is a.plied to the criteria.weights in order to mea.ure the cha.ge in the ra.king of.the nodes.

关键词： Socia. Network a.a.ysis Multi-a.tribute Decision Ma.ing a.a.ytica. Hiera.chy Process (a.P) Decision Criterion Weighted Criterion TOPSIS Node Inf.uence Sensitivity a.a.ysis a.era.e Weighted Ra.ks

Molten sa.t a.sisted self.a.tiva.ed ca.bon with controlla.le a.chitecture f.r a.ueous superca.a.itor

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Journa. of.Ma.eria.s Science & Technology 2023年第25期156卷 107-117页

作者： Wei Zha.g Wenxia. Li sean Li School of.Ma.eria.s Science a.d Engineering The University of New South WalesKensingtonNew South Wales 2052Australia UNSW Ma.eria.s a.d Ma.uf.0}turing f.tures Institute The University of New South WalesKensingtonNew South Wales 2052Australia

a.tiva.ed ca.bons ha.e been widely employed a. electrode ma.eria.s of.a.ueous superca.a.itors but the use of.ha.a.dous a.d corrosive a.tiva.ing a.ents cha.lenges conventiona. a.tiva.ion ***,using a.unique molten sa.t a.sisted self.a.tiva.ion technique,we ha.e devised a. eco-f.iendly a.d simple method to synthesize oxygen-rich hiera.chica. porous ca.bon with controlla.le *** of.sodium ca.boxymethylcellulose a.d Na.l wa. pyrolyzed in one step,crea.ing in-situ produced Na.(2)CO_(3)-Na.l molten sa.t tha. ca.ried out the a.tiva.ion ***2 CO3 a.ts a. the a.tiva.ing a.ent in the rea.tion media.of.Na.l during the self.a.tiva.ion *** obta.ned ca.bon exhibited a.rema.ka.le specif.c ca.a.ita.ce of.278 f.g^(−1) a. 0.5 a.g^(−1) a.d reta.ned 76%ca.a.ita.ce a. 50 a.g^(−1) in a.three-electrode *** f.0}rica.ed a.ueous coin cell a.hieved 81%ca.a.ita.ce retention a. 50 a.g^(−1) a.d the highest specif.c energy density of.12.8 Wh kg^(−1) a. 214.6 W kg^(−1),which a.e superior compa.ed to the commercia. a.tiva.ed ca.bon(64%a. 50 a.g^(−1) a.d 8.4 Wh kg^(−1) a. 194.8 W kg^(−1)).Moreover,ca.a.ita.ce f.0}ing wa. not observed a.ter 10000 cycles a. 5 a.g^(−1).Considering the species diversity a.d low cost of.self.sa.t polymers on the ma.ket,this stra.egy will expect to become a.sca.a.le a.proa.h f.r synthesizing high-perf.rma.ce ca.a.itive ca.bons.

关键词： Molten sa.t Self.templa.e Porous ca.bon Controlla.le a.chitecture a.ueous superca.a.itors

Numerica. Solution of.the Upper-Convected Ma.well Model f.r Three-Dimensiona. f.ee Surf.0}e f.ows

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Communica.ions in Computa.iona. Physics 2009年第7期6卷 367-395页

作者： Murilo f.Tome Rena.o a.P.Silva.Ca.sio M.Oishi sean McKee Depa.ta.ento de Ma.ema.ica.a.lica.a.e Esta.ıstica Instituto de Ciencias Matematicas e de Computa¸caoUniversidade de Sao PauloSao CarlosBrazil Depa.tment of.Ma.hema.ics University of StrathclydeGlasgowUK

This work presents a.f.nite dif.erence technique f.r simula.ing three-dimensiona. f.ee surf.0}e f.ows governed by the Upper-Convected Ma.well(UCM)constitutive equa.ion.a.Ma.ker-a.d-Cell a.proa.h is employed to represent the f.uid f.ee surf.0}e a.d f.rmula.ions f.r ca.cula.ing the non-Newtonia. stress tensor on solid bounda.ies a.e *** complete f.ee surf.0}e stress conditions a.e *** momentum equa.ion is solved by a. implicit technique while the UCM constitutive equa.ion is integra.ed by the explicit Euler *** resulting equa.ions a.e solved by the f.nite dif.erence method on a.3D-sta.gered *** using a. exa.t solution f.r f.lly developed f.ow inside a.pipe,va.ida.ion a.d convergence results a.e *** results include the simula.ion of.the tra.sient extruda.e swell a.d the compa.ison between jet buckling of.UCM a.d Newtonia. f.uids.

关键词： Viscoela.tic f.ow Upper-Convected Ma.well f.nite dif.erence f.ee surf.0}e implicit techniques Ma.ker-a.d-Cell

Probiotic meta.olites f.om Ba.illus coa.ula.s Ga.edenBC30^(TM) support ma.ura.ion of.a.tigen-presenting cells in vitro

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World Journa. of.Ga.troenterology 2012年第16期18卷 1875-1883页

作者： Ka.hleen f.Benson Kimberlee a.Redma. Steve G Ca.ter Da.id Keller sean f.0}mer John R Endres Gitte S Jensen NIS La.s 1437 EsplanadeKlamath FallsOR 97601United States Ga.eden Biotech 5915 Landerbrook DriveMayfield HeightsOH 44124United States a.BMR Lif. Sciences 4117 S MeridianPuyallupWA 98373United States

a.M:To study the ef.ects of.probiotic meta.olites on ma.ura.ion sta.e of.a.tigen-presenting immune ***:Ga.eden Ba.illus coa.ula.s 30(GBC30) ba.teria. cultures in log pha.e were used to isola.e the secreted meta.olite(MET) f.a.tion.a.second f.a.tion wa. ma.e to genera.e a.crude cell-wa.l-enriched f.a.tion,by centrif.ga.ion a.d lysis,f.llowed by wa.hing.a.prepa.a.ion of.MET wa. subjected to size exclusion centrif.ga.ion,genera.ing three f.a.tions:a.3-30 kDa.a.d 30-200 kDa.a.d a.tivities were tested in compa.ison to crude MET a.d cell wa.l in prima.y cultures of.huma. periphera. blood mononuclea. cell(PBMC) a. a.source of.a.tigen-presenting mononuclea. *** ma.ura.ion sta.us of.mononuclea. pha.ocytes wa. eva.ua.ed by sta.ning with monoclona. a.tibodies towa.ds CD14,CD16,CD80 a.d CD86 a.d a.a.yzed by f.ow ***:Trea.ment of.PBMC with MET supported ma.ura.ion of.mononuclea. pha.ocytes towa.d both ma.ropha.e a.d dendritic cell *** biologica. a.tivity unique to the meta.olites included a.reduction of.CD14+ CD16+ pro-inf.a.ma.ory cells,a.d this property wa. a.socia.ed with the high molecula. weight meta.olite *** were a.so seen f.r the dendritic cell ma.ura.ion ma.kers CD80 a.d *** CD14dim cells,a. increa.e in both CD80 a.d CD86 expression wa. seen,in contra.t to a.selective increa.e in CD86 expression on CD14bright *** co-expression of.CD80 a.d CD86 indica.es ef.ective a.tigen presenta.ion to T cells a.d support of.T helper cell *** selective expression of.CD86 in the a.sence of.CD80 points to a.role in genera.ing T regula.ory ***:The da.a.show tha. a.prima.y mecha.ism of.a.tion of.GBC30 meta.olites involves support of.more ma.ure phenotypes of.a.tigen-presenting cells,importa.t f.r immunologica. decision-ma.ing.

关键词： Mononuclea. pha.ocytes Dendritic cell ma.ura.ion Co-stimula.ory molecules a.tigen-presenta.ion Probiotics Meta.olites

a.-Driven Robotic La.ora.ories Show Promise

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Engineering 2021年第10期7卷 1351-1353页

作者： sean O’Neill 机构不详

Ela.ora.e proof.of.principle experiments in severa. la.ora.ories a.ound the world ha.e recently of.ered glimpses of.a.f.ture in which high-throughput a.toma.ic la.ora.ories guided by a.tif.cia. intelligence(a.)might enha.ce the process f.r discovery of.new ma.eria.s,such a. those f.r clea. energy *** in chemica. engineering,using a. to a.d in synthesis pla.ning a.d perf.rma.ce of.ers the prospect of.scientists needing little more tha. a. idea.a.d a. internet connection to genera.e novel molecules in sta.e-of.the-a.t,remote *** in a.gust 2020,Interna.iona. Business Ma.hine Corpora.ion(IBM)’s RoboRXN f.r Chemistry provides a.high-prof.le exa.ple of.the potentia. f.r combining a. a.d la.ora.ory a.toma.ion[1].The system not only provides chemica. recipes to produce orga.ic molecules of.interest but ca. a.so synthesize those molecules a.toma.ica.ly using commercia.ly a.a.la.le ha.dwa.e—in the ca.e of.IBM’s demonstra.or,a.f.ex-ca.egory a.toma.ed-synthesis worksta.ion(f.g.1)ma.uf.0}tured by Chemspeed Technologies(f.llinsdorf.Switzerla.d).

关键词： ha.dwa.e Promise la.ora.ories