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Preparation on the basis of Trichoderma asperellum in the system of biological protection of wheat from Fusarium ear scab

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浙江大学学报(农业与生命科学版) 2004年第4期 44-45页

作者： Kolombet L V,Sokolov M S,Chuprina V P,Schisler D A,samuels G J State Research Center for Applied Microbiology Obolensk Moscow Region Russia Research Center of Toxicology and Hygienic Regulation of Biological Products Serpukhov Russia All-Russia Research Institute of Biological Plant Disease Control Krasnodar Russia USDA-ARS nCAUR Peoria Illinois USA USDA-ARS Systematic Botany and Mycology Lab. Beltsville USA

During the last century, as the area of wheat grown under advanced grain husbandry has increased worldwide, so too has the importance of Fusarium ear scab (FES) (synonym, Fusarium head blight) caused by several species of the fungus Fusarium. Yield losses due to FES can total 20%-40% and more depending on climatic conditions. During the last twenty years epidemics of FES in cereals have become chronic all over the world, including the United States and Russia. The most destructive of these were observed in 1982, 1986, 1990-1996 in USA and in the south of Russia in 1982, 1984, 1988, 1992. The harmful effect of FES is manifested not only in reduced grain yields, but also in the contamination of grains and grain products with mycotoxins, such as deoxynivalenol (DOn) and its derivatives (3-alfa acetyl-DOn, 15-alfa acetyl-DOn), T-2 toxin and zearalenone. Standard means to control FES (cultural control methodologies, chemical pesticides, and FES resistant varieties) have little effect or are not practical and rarely reduce the accumulation of mycotoxins in grain. We have developed a new technique to reduce FES using biological preparations. The technique utilizes wheat seed pretreatment with a biofungicide “Mycol” in combination with spraying wheat plants during flowering with a yeast preparation. Technology for production of Mycol on the basis of Trichoderma asperellum strain GJS 03-35 (systematics by samuels) has been developed. This strain shows hyperparasitic activity against a wide spectrum of plant pathogens, including Fusarium graminearum, a causative agent of FES in wheat. Experiments conducted in the United States demonstrated that spraying wheat plants during flowering with the patented yeast Cryptococcus nodaensis OH 182.9 (nRRL Y-30216) reliably reduces FES development. Tests of the Mycol preparation and the yeast OH 182.9 (EOD) have been performed on the spring wheat “Ivolga” in greenhouse conditions (the Moscow region) and on the winter wheat “K

关键词： Fusarium ear scab (FES) Fusarium head blight mycotoxins deoxynivalenol (DOn) biofungicide “Mycol” Trichoderma asperellum strain GJS 03-35 yeast Cryptococcus nodaensis OH 182.9 (nRRL. Y-30216) biocontrol

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Phylogeny and taxonomic revision of Thelonectria discophora(Ascomycota,Hypocreales,nectriaceae)species complex

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Fungal Diversity 2015年第1期70卷 1-29页

作者： Catalina Salgado-Salazar Amy Y.Rossman Gary J.samuels Yuuri Hirooka Romina M.Sanchez Priscila Chaverri Department of Plant Science and Landscape Architecture University of Maryland2112 Plant Sciences BuildingCollege ParkMD 20742USA Systematic Mycology and Microbiology Laboratory USDA-ARSBeltsvilleMD 20705USA Biodiversity(Mycology) Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOttawaOntario K1A 0C6Canada Centro de Recursos Renovables de la Zona Semiarida-Cerzos Conicet Universidad Nacional del SurCamino La Carrindanga Km7B8000FWB Bahía BlancaProvincia de Buenos AiresArgentina Escuela de Biología Universidad de Costa RicaApartado11501-2060 San PedroSan JoséCosta Rica

Specimens regarded as Thelonectria discophora(Thelonectria,nectriaceae,Hypocreales)constitute a conspicuous group of saprobic fungi on decaying plant material,characterized by red perithecia each with a broad mammiform(nipple-like)*** asexual state is characterized by a cylindrocarpon-like morphology,with 3–5 septate macroconidia,unicellular microconidia and chlamydospores that are rarely produced in *** the past,*** was regarded as one species with a wide geographic ***,a recent study rejected the monophyly and cosmopolitan distribution of this species,and showed the existence of at least 16 cryptic species distributed in three main *** combining the results of phylogenetic analyses of six nuclear loci and morphological studies,we revised the taxonomy of the *** species complex,resulting in the description of 12 new species and four new combinations based on historic *** though molecular phylogenetic analyses strongly support the segregation of these species,and are in agreement with previous studies,individual diagnostic morphological characters for each species could not be ***,discrete morphological traits corresponding to each of the three main groups of species were *** could be differentiated based on the average values of morphological traits as well as the presence/absence of characteristic asexual propagules and colony growth at ***,illustrations are provided for the recognized species.

关键词： new species Rubus canker Species concept Taxonomy

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《生物语言学视角下的音系结构》介绍

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当代语言学 2015年第1期17卷 120-123页

作者： Bridege D.samuels 胡伟南开大学外国语学院天津市300071

纵观生成语言学的历史，决定语言机制的问题是“自上而下”处理的，普遍语法在生成语言学中的作用到底有多大？而最简方案理论是“自下而上”处理的，普遍语法又能限制在多小的范围内（Chomsky 2007：3）？***的《生物语言学视角下的音... 详细信息

纵观生成语言学的历史，决定语言机制的问题是“自上而下”处理的，普遍语法在生成语言学中的作用到底有多大？而最简方案理论是“自下而上”处理的，普遍语法又能限制在多小的范围内（Chomsky 2007：3）？***的《生物语言学视角下的音系结构》（Phonological Architecture：A Biolinguistic Approach）便致力于回答这一问题。该书于2011年由牛津大学出版社出版，共七章，以下简要介绍各章内容。

关键词：生物语言学音系结构牛津大学出版社生成语言学 “自上而下” 普遍语法语言机制自下而上

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用于热气体过滤的改进纤维

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产业用纺织品 1998年第5期16卷 38-42页

作者： S.L.samuels 吴英

1 引言众所周知,为清洁空气对汽车尾气排放立法,竞争迫使汽车公司研究降低汽车有害气体排放的技术,其中从汽车尾气中将固体粒子过滤分离是关键技术。从60年代初开发芳香族酰胺纤维nomex^((R))和70年代开发Teflon^((R))PTFE纤维开始,杜... 详细信息

1 引言众所周知,为清洁空气对汽车尾气排放立法,竞争迫使汽车公司研究降低汽车有害气体排放的技术,其中从汽车尾气中将固体粒子过滤分离是关键技术。从60年代初开发芳香族酰胺纤维nomex^((R))和70年代开发Teflon^((R))PTFE纤维开始,杜邦公司一直致力于发展控制污染和回收产品用的耐高温(>150℃)过滤介质,以满足特殊用户的需求。虽然原先的芳香族聚酞胺纤维(2.2dtex)和 PTFE纤维(7.4 dtex)的性能可满足多种应用的需要,但客户有时发现传统基布问题的解决办法(例如提高毡的基本重量、涂层、薄膜)既不能满足新的要求,又会产生一些难以接受的负效应(如压降高、寿命短)。多年来。

关键词：汽车尾气过滤纤维聚酰胺超细纤维

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The Longibrachiatum Clade of Trichoderma:a revision with new species

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Fungal Diversity 2012年第4期55卷 77-108页

作者： Gary J.samuels Adnan Ismaiel Temesgen B.Mulaw George Szakacs Irina S.Druzhinina Christian P.Kubicek Walter M.Jaklitsch United States Department of Agriculture Agricultural Research ServiceSystematic Mycology&Microbiology LaboratoryB-010a10300 Baltimore AveBeltsvilleMD 20705USA Center for Environmental Science Institute of Marine and Environmental TechnologyUniversity of MarylandBaltimoreMD 21201USA Department of Applied Biotechnology and Food Science Budapest University of Technology and Economics(BUTE)1111 BudapestGellért ter 4Hungary Vienna University of Technology Institute of Chemical EngineeringGetreidemarkt 9/1661060 WienAustria Department of Systematic and Evolutionary Botany Faculty Centre of BiodiversityUniversity of ViennaRennweg 141030 ViennaAustria

The Longibrachiatum Clade of Trichoderma is *** new species are described(***,***,***,***,***,***,***,***).The twenty-one species known to belong to the Longibrachiatum Clade are included in a synoptic *** parareesei and *** are redescribed based on new collections or additional *** teleomorphs are reported for *** and *** described species are annotated.

关键词： Hypocrea Hypocreaceae Hypocreales Endophyte Medical mycology Revision Systematics Taxonomy Soil fungi Biogeography

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Parkin Somatic Mutations Link Melanoma and Parkinson's Disease

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Journal of Genetics and Genomics 2016年第6期43卷 369-379页

作者： Lotan Levin Shani Srour Jared Gartner Oxana Kapitansky nouar Qutob Shani Dror Tamar Golan Roy Dayan Ronen Brener Tamar Ziv Mehdi Khaled Ora Schueler-Furman Yardena samuels Carmit Levy Department of Human Genetics and Biochemistry Sackler Faculty of MedicineTel Aviv University Surgery Branch National Cancer InstituteNIH Department of Molecular Cell Biology Weizmann Institute of Science Oncology Institute Edith Wolfson Medical Center Technion Smoler Proteomics Center Faculty of BiologyTechnion e Israel Institute of Technology Institut Gustave Roussy INSERM Department of Microbiology and Molecular Genetics Institute for Medical Research Israel-CanadaFaculty of MedicineHebrew University

Epidemiological studies suggest a direct link between melanoma and Parkinson＇s disease（PD）; however, the underlying molecular basis is unknown. Since mutations in Parkin are the major driver of early-onset PD and Parkin was recently reported to play a role in cancer development, we hypothesized that Parkin links melanoma and PD. By analyzing whole exome/genome sequencing of Parkin from 246 melanoma patients, we identified five non-synonymous mutations, three synonymous mutations, and one splice region variant in Parkin in3.6% of the samples. In vitro analysis showed that wild-type Parkin plays a tumor suppressive role in melanoma development resulting in cell-cycle arrest, reduction of metabolic activity, and apoptosis. Using a mass spectrometry-based analysis, we identified potential Parkin substrates in melanoma and generated a functional protein association network. The activity of mutated Parkin was assessed by protein structure modeling and examination of Parkin E3 ligase activity. The Parkin-E28 K mutation impairs Parkin ubiquitination activity and abolishes its tumor suppressive effect. Taken together, our analysis of genomic sequence and in vitro data indicate that Parkin is a potential link between melanoma and Parkinson＇s disease. Our findings suggest new approaches for early diagnosis and treatment against both diseases.

关键词： Melanoma Parkinson's disease Parkin Mutation

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Recent Trends and Future Predictions until 2060 of Urban Warming in Four Israeli Cities Employing the RegCM Climate Model

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American Journal of Climate Change 2016年第4期5卷 464-484页

作者： Hofit Itzhak-Ben-Shalom Rana samuels Oded Potchter Pinhas Alpert The Porter School of Environmental Studies Tel-Aviv University Tel-Aviv Israel Department of Geosciences Tel-Aviv University Tel-Aviv Israel Department of Geography and the Human Environment Tel-Aviv University and Department of Geography Beit-Berl Academic College Tel-Aviv Israel

During periods of global warming (GW), expected increases in urban temperatures can have adverse impacts on city climate and thermal discomfort due to combined urban and global warming effects. The different climates in four cities in Israel are studied for the purpose of differentiating global vs. urban warming. Trends in urban and nearby rural areas were compared in order to estimate the urbanization effect on the local climate zones. Daily 06:00 and 15:00 Local Time (LT) temperatures for July 1980-2014 were investigated. The linear relationship between the urban warming and population growth observed in present climate data is assumed to continue into the near future. The Regional Climate Model (RegCM) temperature trends into the 21st century are assumed to represent primarily the GW because of the relatively coarse grid interval of 25 km. Hence, this study first differentiates between global and local warming past trends, and then uses this past result to make future projections that consider both factors. A unique feature of this study is the large climatic variety over Israel—a small country that encompasses no less than 5 different K?ppen climatic zones. The urban minus rural temperature (1980-2014) changes, ΔTu-r, show more intense warming in the afternoon in all 4 cities. For instance, in Jerusalem and Eilat, the ΔTu-r has increased by ~1.2°C. Following the RegCM predictions, by 2060 with “no population growth”, this temperature increase is expected to continue, by 1.114°C and 1.119°C, respectively. If, however, these cities grow rapidly, air temperature will increase by 2.937°C - 4.129°C and 2.778°C - 3.939°C, respectively.

关键词： Urban Warming Climatic Change Future Prediction Global Warming Israeli Cities

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Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ

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World Journal of Biological Chemistry 2017年第3期8卷 163-174页

作者： Amy L samuels Alison Louw Reza Zareie Evan Ingley Cell Signalling Group Harry Perkins Institute of Medical Research and Centre for Medical Researchthe University of Western Australia Proteomics International Laboratories Ltd Proteowa Pty Ltd SABCMurdoch University Cell Signalling Group School of Veterinary and Health SciencesMurdoch University

AIM To identify and characterize the effect of phosphorylation on the subcellular localization of *** HEK293 T cells were treated with calyculin A, staurosporin or phorbol 12-myristate 13-acetate(PMA). Cells were transfected with eG FP-tagged Ankrd54 with or without Lyn tyrosine kinase(wild-type, Y397 F mutant, or Y508 F mutant). The subcellular localization was assessed by immunofluorescence imaging of cells, immunoblotting of subcellular fractionations. The phosphorylation of Ankrd54 was monitored using Phos-tagT M gel retardation. Phosphorylated peptides were analysed by multiplereaction-monitoring(MRM) proteomic *** Activation of PKC kinases using PMA promoted nuclear export of Ankrd54 and correlated with increased Ankrd54 phosphorylation, assayed using Phos-tag TM gel retardation. Co-expression of an active form of the PKCδisoform specifically promoted both phosphorylation and cytoplasmic localization of Ankrd54, while PKCδ, Akt and PKA did not. Alanine mutation of several serine residues in the amino-terminal region of Ankrd54(Ser14, Ser17, Ser18, Ser19) reduced both PMA induced cytoplasmic localization and phosphorylation of Ankrd54. Using MRM proteomic analysis, phosphorylation of the Ser18 residue of Ankrd54 was readily detectable in response to PMA stimulation. PMA stimulation of cells co-expressing Ankrd54 and Lyn tyrosine kinase displayed increased coimmunoprecipitation and enhanced co-localization in the *** We identify phosphorylation by PKCδ as a major regulator of nuclear-cytoplasmic shuttling of Ankrd54, and its interaction with the tyrosine kinase Lyn.

关键词： Ankrd54 PKC Lyn Btk Phosphorylation

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Water Contamination Modeling—A Review of the State of the Science

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Journal of Water Resource and Protection 2013年第2期5卷 142-155页

作者： Rakesh Bahadur David E. Amstutz William B. samuels Center for Water Science and Engineering Science Applications International Corporation McLean USA

This paper reports on the current state of surface water and ocean contamination models—based on the needs of US Government agencies, their Information Technology (IT) systems, and business processes. In addition, down-selection and evaluation criteria were applied in a two-step process. In Step 1, sixty five surface water and ocean models were identified and researched. In Step 2, the following criteria were explored for each model: 1) model environment (river, lake estuary, coastal ocean and watershed);2) degree of analysis (screening model intermediate model, advanced model);3) availability (public domain, proprietary);4) temporal variability (steady state or time variable/dynamic);5) spatial resolution (one, two or three dimensional);6) processes (flow, transport, both flow and transport in an integrated system);7) water quality (chemical, biological, radionuclides, sediment);and 8) support (user support/training available, user manuals/documents available).

关键词： Watershed Coastal Ocean Rivers Modeling Simulation

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Global River Basin Modeling and Contaminant Transport

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American Journal of Climate Change 2013年第2期2卷 138-146页

作者： Rakesh Bahadur Christopher Ziemniak David E. Amstutz William B. samuels Center for Water Science and Engineering Science Applications International Corporation McLean USA

Using geographic information system techniques, elevation derived datasets such as flow accumulation, flow direction, hillsope and flow length were used to delineate river basin boundaries and networks. These datasets included both HYDRO1K (based on 1 kmresolution DEM) and HydroSHEDs (based on100 meterShuttle Radar Topography Mission). Additional spatial data processing of global landuse and soil type data were used to derive grids representing soil depth, texture, hydraulic conductivity, water holding capacity, and curve number. These grids were input to the Geospatial Stream Flow model to calculate overland flow (both travel time and velocity). The model was applied to river basins across several continents to calculate river discharge and velocity based on the use of satellite derived rainfall estimates, numerical weather forecast fields, and geographic data sets describing the land surface. Model output was compared to historical stream gauge observations as a validation step. The stream networks with associated discharge and velocity are used as input to a riverine water contamination model.

关键词： Hydrology Geographic Information Systems Surface Water

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