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Phenotypic characterization and fine mapping of mps1,a premature leaf senescence mutant in rice (Oryza sativa L.)

Journal of Integrative Agriculture 2016年第9期15卷 1944-1954页

作者： LIU Zhong-xian CUI Yu WANG Zhong-wei XIE Yuan-hua sang xian-chun YANG Zheng-lin ZHANG Chang-wei ZHAO Fang-ming HE Guang-hua LING Ying-hua Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute Southwest University

Leaves play a key role in photosynthesis in rice plants. The premature senescence of such plants directly reduces the accumulation of photosynthetic products and also affects yield and grain quality significantly and negatively. A novel premature senescence mutant, mps1（mid-late stage premature senescence 1）, was identified from a mutant library consisting of ethyl methane sulfonate（EMS） induced descendants of Jinhui 10, an elite indica restorer line of rice. The mutant allele, mps1, caused no phenotypic differences from the wild type（WT）, Jinhui 10, but drove the leaves to turn yellow when mutant plants grew to the tillering stage, and accelerated leaf senescence from the filling stage to final maturation. We characterized the agronomic traits, content of photosynthetic pigments and photosynthetic efficiency of mps1 and WT, and fine-mapped MPS1. The results showed that the MPS1-drove premature phenotype appeared initially on the leaf tips at the late tillering stage and extended to the middle of leaves during the maturing stage. Compared to the WT, significant differences were observed among traits of the number of grains per panicle（–31.7%） and effective number of grains per panicle（–38.5%） of mps1 individuals. Chlorophyll contents among the first leaf from the top（Top 1st）, the second leaf from the top（Top 2nd） and the third leaf from the top（Top 3rd） of mps1 were significantly lower than those of WT（P〈0.05）, and the levels of photosynthetic efficiency from Top 1st to the forth leaf from the top（Top 4th） of mps1 were significantly lower than those of WT（P〈0.01）. Results from the genetic analysis indicated that the premature senescence of mps1 is controlled by a recessive nuclear gene, and this locus, MPS1 is located in a 37.4-kb physical interval between the markers Indel145 and Indel149 on chromosome 6. Genomic annotation suggested eight open reading frames（ORFs） within this physical region. All of these results will provide informative references for the furthe

关键词： rice(Oryza sativa L.) premature senescence fine mapping mps1

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Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

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Journal of Integrative Agriculture 2012年第10期11卷 1574-1579页

作者： REN De-yong LI Yun-feng WANG Zeng XU Fang-fang GUO Shuang ZHAO Fang-ming sang xian-chun LING Ying-hua HE Guang-hua Key Laboratroy of Application and Safety Control of Genetically Modified Crops Rice Research Institute Southwest University

In this study, a rice spikelet mutant, multi-floret spikelet 1 （mfsl）, which was derived from ethylmethane sulfonate （EMS）- treated Jinhui 10 （Oryza sativa L. ssp. indica） exhibited pleiotropic defects in spikelet development. The mfsl spikelet displayed degenerated the empty glume, elongated the rachilla, the extra lemma-like organ and degraded the palea. Additionally, mfsl flowers produced varied numbers of inner floral organs. The genetic analysis revealed that the mutational trait was controlled by a single recessive gene. With 401 recessive individuals from the F2 segregation population, the MFS1 gene was finally mapped on chromosome 5, an approximate 350 kb region. The present study will be useful for cloning and functional analysis of MFS1, which would facilitate understanding of the molecular mechanism involved in spikelet development in rice.

关键词： empty glume gene mapping mfsl mutant rice (Oryza sativa L.) spikelet

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Less hairy leaf 1,an RNaseH-like protein,regulates trichome formation in rice through auxin

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Journal of Integrative Agriculture 2023年第1期22卷 31-40页

作者： CHEN Hong-yan ZHU Zhu WANG Xiao-wen LI Yang-yang HU Dan-ling ZHANG Xue-fei JIA Lu-qi CUI Zhi-bo sang xian-chun Key Laboratory of Application and Safety Control of Genetically Modified Crops Rice Research InstituteAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China

The trichomes of rice leaves are formed by the differentiation and development of epidermal *** trichomes play an important role in stress resistance and protection against direct ultraviolet ***,the development of rice trichomes remains poorly *** this study,we conducted ethylmethane sulfonate(EMS)-mediated mutagenesis on the wild-type(WT)indica rice‘Xida 1B’.Phenotypic analysis led to the screening of a mutant that is defective in trichome development,designated lhl1(less hairy leaf 1).We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome *** locus LOC_Os02g25230 was identified as the candidate gene by *** constructed RNA interference(LHL1-RNAi)and overexpression lines(LHL1-OE)to verity the candidate *** leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant,whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT,although the number of trichomes was significantly *** real-time PCR(RT-qPCR)analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the *** response analysis revealed that LHL1 expression was affected by *** results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.

关键词： rice trichome gene cloning auxin

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YGL9, encoding the putative chloroplast signal recognition particle 43 kDa protein in rice, is involved in chloroplast development

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Journal of Integrative Agriculture 2016年第5期15卷 944-953页

作者： WANG Zhong-wei ZHANG Tian-quan XING Ya-di ZENG Xiao-qin WANG Ling LIU Zhong-xian SHI Jun-qiong ZHU Xiao-yan MA Ling LI Yun-feng LING Ying-hua sang xian-chun HE Guang-hua Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops Rice Research Institute Southwest University Chongqing 400715 P.R. China

The nuclear-encoded light-harvesting chlorophyll a/b-binding proteins（LHCPs） are specifically translocated from the stroma into the thylakoid membrane through the chloroplast signal recognition particle（cp SRP） pathway. The cp SRP is composed of a cp SRP43 protein and a cp SRP54 protein, and it forms a soluble transit complex with LHCP in the chloroplast stroma. Here, we identified the YGL9 gene that is predicted to encode the probable rice cp SRP43 protein from a rice yellow-green leaf mutant. A phylogenetic tree showed that an important conserved protein family, cp SRP43, is present in almost all green photosynthetic organisms such as higher plants and green algae. Sequence analysis showed that YGL9 comprises a chloroplast transit peptide, three chromodomains and four ankyrin repeats, and the chromodomains and ankyrin repeats are probably involved in protein-protein interactions. Subcellular localization showed that YGL9 is localized in the chloroplast. Expression pattern analysis indicated that YGL9 is mainly expressed in green leaf sheaths and leaves. Quantitative real-time PCR analysis showed that the expression levels of genes associated with pigment metabolism, chloroplast development and photosynthesis were distinctly affected in the ygl9 mutant. These results indicated that YGL9 is possibly involved in pigment metabolism, chloroplast development and photosynthesis in rice.

关键词： Oryza sativa yellow-green leaf gene cpSRP43 light-harvesting chlorophyll a/b-binding protein

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Genetic Analysis and Gene Mapping of a Rice Tiller Angle Mutant tac2

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Rice science 2009年第4期16卷 323-326页

作者： FANG Li-kui sang xian-chun YANG Zheng-lin LIN Ying-hua WAN Nan HE Guang-hua Rice Research Institute~Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture Southwest University Chongqing 400716 China

Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller anglo controlling 2 （tac2） mutant was obtained from a restorer line Jinhui 10 by ethyl methane sulphonate mutagenesis. The tac2 mutant displayed normal phenotype at the seedling stage and the tiller angle significantly increased at the tillering stage, A preliminary physiological research indicated that the mutant was sensitive to GA. Thus, it is speculated that TAC2 and TAC1 might control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene and was located on chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 cM and 16,7 cM, respectively.

关键词： rice tiller angle mutant gene mapping genetics

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一种高效的蚕豆染色体加倍方法探讨

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河南农业科学 2013年第6期42卷 42-45页

作者：王晓雯桑贤春西南大学农学与生物科技学院南方山地农业教育部工程研究中心重庆400716

为解决传统植物染色体加倍方法效率低、费工费时、中期分裂相细胞少等问题,采用4种质量浓度秋水仙素对蚕豆侧根进行处理,检测加倍效果。结果表明,以0.5～1.0cm长的蚕豆侧根为材料,用0.10mg/g的秋水仙素处理48h时,根尖的膨大率最高,为92.... 详细信息

为解决传统植物染色体加倍方法效率低、费工费时、中期分裂相细胞少等问题,采用4种质量浓度秋水仙素对蚕豆侧根进行处理,检测加倍效果。结果表明,以0.5～1.0cm长的蚕豆侧根为材料,用0.10mg/g的秋水仙素处理48h时,根尖的膨大率最高,为92.94%,膨大根尖中部至根冠区的细胞诱导率为28.20%,显著高于中部至伸长区。侧根平均8条,与传统的主根加倍方法相比,工作效率提高了8倍,且显微镜下观测到的中期分裂相细胞更多。因此,该方法不仅适合植物染色体加倍试验的教学,也为其他植物的染色体加倍提供了借鉴。

关键词：秋水仙素蚕豆侧根染色体加倍

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植物防御素基因alfAFP(***)在毕赤酵母中的分泌表达及对水稻病原菌的抑制

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中国农业科学 2009年第3期42卷 869-875页

作者：李平桑贤春裴炎何光华西南大学水稻研究所农业部西南作物遗传改良与育种重点开放实验室

【目的】大多数的植物防御素对不同的病原菌具有抗菌活性,其中来自苜蓿种子的防御素alfAFP的转基因土豆和番茄已经被证明对病原菌具有强抗病性。进一步研究该基因对稻瘟病、纹枯病、白叶枯病三大重要病害的抑制作用,对水稻抗病基因工程... 详细信息

【目的】大多数的植物防御素对不同的病原菌具有抗菌活性,其中来自苜蓿种子的防御素alfAFP的转基因土豆和番茄已经被证明对病原菌具有强抗病性。进一步研究该基因对稻瘟病、纹枯病、白叶枯病三大重要病害的抑制作用,对水稻抗病基因工程研究具有重要的意义。【方法】本试验将alfAFP构建到表达载体pPIC9K上,电转化毕赤酵母(GS115),再进行蛋白质的分泌性诱导表达,最后进行体外重组蛋白对水稻病菌的抑菌活性检测。【结果】通过15%的SDS-PAGE检测,得到大约6.5kD的重组蛋白。alfAFP融合蛋白对水稻纹枯病、稻瘟病和白叶枯病均具有一定的抑制活性,其中对纹枯病的作用最为明显。【结论】alfAFP在酵母中成功诱导表达,并对水稻测试病菌具有一定的抑制活性,预示着alfAFP防御素基因在水稻抗病基因工程中具有潜在的应用价值。

关键词：植物防御素alfAFP 毕赤酵母抗菌活性稻瘟病白叶枯病纹枯病

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A Multi-faceted View of the X-Ray Spectral Variability in Seyfert Galaxy Ark 120

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Research in Astronomy and Astrophysics 2025年第1期25卷 228-239页

作者： Lu-Xin Ren Jun-xian Wang Jia-Lai Kang CAS Key Laboratory for Research in Galaxies and Cosmology Department of AstronomyUniversity of Science and Technology of China School of Astronomy and Space Science University of Science and Technology of China

Utilizing a range of techniques including multi-band light curves,softness ratio analysis,structure functions,rms spectra,cross-correlation functions,and ratios of spectra from different intervals,we present a comprehensive study of the complex X-ray spectral variability in Seyfert 1 galaxy Ark 120,through re-analyzing its six XMM-Newton observations taken between 2003 and *** find a clear“softer-when-brighter”trend in the 2–10 ke V power-law component over long timescales,with this trend being timescale dependent,as it is much weaker on shorter timescales,similar to that previously detected in NGC ***,a rare“harder-when-brighter”trend is observed during one exposure,indicating dynamic changes in the spectral variability behavior of the power-law *** exceptional exposure,with the spectral variability indeed marked by a power-law pivoting at an unusually low energy of～2 ke V,suggests intricate variations in the thermal Comptonization processes within the ***,when the data below 2 ke V are included,we identify that the soft excess component adds significant complexity to the spectral variability,such as evidenced by a transition from“harder-when-brighter”to“softer-when-brighter”during another single *** extra complexity arises because the variability of the soft excess sometimes follows and sometimes does not follow the changes in the power-law *** findings underscore the necessity of applying multiple analytic techniques to fully capture the multifaceted spectral variability of AGNs.

关键词： galaxies: active galaxies: Seyfert X-rays: galaxies

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水稻类病斑早衰突变体lmps1的表型鉴定与基因定位

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作物学报 2019年第1期45卷 46-54页

作者：夏赛赛崔玉李凤菲谭佳谢园华桑贤春凌英华西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室重庆400715

经甲基磺酸乙酯(EMS)诱变优良籼型水稻恢复系缙恢10号,获得一个稳定遗传的水稻类病斑早衰突变体lmps1(lesion mimic and premature senescence 1)。该突变体苗期表型正常,分蘖早期出现褐色类病斑,且斑点数目随植株生长而增多,孕穗期叶... 详细信息

经甲基磺酸乙酯(EMS)诱变优良籼型水稻恢复系缙恢10号,获得一个稳定遗传的水稻类病斑早衰突变体lmps1(lesion mimic and premature senescence 1)。该突变体苗期表型正常,分蘖早期出现褐色类病斑,且斑点数目随植株生长而增多,孕穗期叶片开始萎黄衰老。与野生型相比,突变体lmps1的每穗总粒数下降8%(P<0.05),株高、穗长、有效穗数、每穗实粒数、结实率以及千粒重分别下降14.3%、24.3%、27.2%、50%、45.7%与14.5%,差异均达极显著水平(P<0.01)。遮光处理表明,突变体lmps1的类病斑性状受光照诱导。孕穗期叶片光合色素含量下降且光合效率降低, H2O2含量增加,抗氧化酶SOD和CAT的活性显著降低。透射电镜观察结果显示,突变体lmps1叶肉细胞中叶绿体数目减少,叶绿体的类囊体片层结构损伤降解。qRT-PCR结果显示,突变体lmps1中防卫反应相关基因除POX22.3表达量降低外,POC1、PAL、PBZ1、PR1、NPR1、PR5表达量均极显著高于野生型。遗传分析表明突变体lmps1的类病斑早衰性状受1对隐性核基因控制,利用西农1A与突变体lmps1杂交所得F2群体中的突变株,将目标基因定位于第7染色体长臂端粒附近约167.3 kb的物理区段内。

关键词：水稻(Oryza sativa L.) 类病斑早衰突变体 (lmps1) 表型鉴定基因定位

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水稻新黄绿叶基因YGL9的分子定位

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作物学报 2015年第7期41卷 989-997页

作者：张天泉郭爽邢亚迪杜丹桑贤春凌英华何光华西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室重庆400716

叶色突变体是研究高等植物光合作用、叶绿素代谢途径、叶绿体结构与功能分子机制的理想材料。本研究从EMS(ethyl methane sulfonate)处理的缙恢10号(Oryza sativa ***)诱变群体中发现了一个苗期呈现黄绿色、抽穗期渐变为淡绿色的叶色突... 详细信息

叶色突变体是研究高等植物光合作用、叶绿素代谢途径、叶绿体结构与功能分子机制的理想材料。本研究从EMS(ethyl methane sulfonate)处理的缙恢10号(Oryza sativa ***)诱变群体中发现了一个苗期呈现黄绿色、抽穗期渐变为淡绿色的叶色突变体,命名为yellow green leaf 9(ygl9)。与野生型相比,ygl9苗期和分蘖期光合色素极显著降低,抽穗期光合色素显著降低,气孔长度、气孔导度和蒸腾速率极显著增加,净光合速率无明显变化。透射电镜观察表明,ygl9的嗜锇小体增多、基粒模糊、基质片层减少且疏松,但叶绿体结构基本完整。遗传分析显示该突变性状受1对隐性核基因调控。利用西农1A/ygl9 F2群体中的759株隐性单株,最终将YGL9定位在第3染色体短臂SSR标记S03-1和In Del标记Ind03-19之间,遗传距离分别为0.13 c M和0.07 c M,物理距离为63 kb。本研究为YGL9基因的克隆和功能分析奠定了基础。

关键词：水稻(Oryza sativa L.) 黄绿叶突变体遗传分析基因定位

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