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Synthesis and crystal structure study of 2′-Se-adenosine-derivatized DNA

Science China Chemistry 2010年第1期53卷 78-85页

作者： salon jozef Department of Chemistry Georgia State University

The selenium derivatization of nucleic acids is a novel and promising strategy for 3D structure determination of nucleic *** can serve as an excellent anomalous scattering center to solve the phase problem,which is one of the two major bottlenecks in macromolecule X-ray *** other major bottleneck is *** has been demonstrated that the incorporated selenium functionality at the 2′-positions of the nucleosides and nucleotides is stable and does not cause significant structure ***,it was observed that the 2′-Se-derivatization could facilitate crystallization of oligonucleotides with fast crystal growth and high diffraction ***,we describe a convenient synthesis of the 2′-Se-adenosine phosphoramidite,and report the first synthesis and X-ray crystal structure determination of the DNA containing the 2′-Se-A *** 3D structure of 2′-Se-A-DNA decamer 5′-GTACGCGT(2′-Se-A)C-3′2 was determined at 1.75 ? resolution,the 2′-Se-functionality points to the minor groove,and the Se-modified and native structures are virtually ***,we have observed that the 2′-Se-A modification can greatly facilitate the crystal growth with high diffraction *** conjunction with the crystallization facilitation by the 2′-Se-U and 2′-Se-T,this novel observation on the 2′-Se-A functionality suggests that the 2′-Se moiety is sole responsible for the crystallization facilitation and the identity of nucleobases does not influence the crystal growth significantly.

关键词： selenium derivatization nucleic acid adenosine X-ray crystallography structure determination

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Chemistry and Structural Biology of Selenium- and Tellurium-Nucleic Acids

Chemistry and Structural Biology of Selenium- and Tellurium-...

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中国化学会第27届学术年会

作者： jozef salon Julianne Caton-Williams Abdalla Hassan Department of Chemistry ＆ Department of Biology Georgia State University

＜正＞Via chemogenetic and chemical synthetic strategies, recently my laboratory pioneered and has developed atom-specific substitution of nucleic acid oxygen with selenium and tellurium, which are

关键词： Selenium- and tellurium-derivatization nucleic acids X-ray crystal structure and function studies

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Nucleic Acid Chemistry and Structural Biology for Disease Mechanism Study and Drug Discovery

Nucleic Acid Chemistry and Structural Biology for Disease Me...

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中国化学会第27届学术年会

作者： jozef salon Julianne Caton-Williams Abdalla Hassan Department of Chemistry ＆ Department of Biology Georgia State University

＜正＞3D structure determination and study of nucleic acids and protein-nucleic acid complexes have enormous importance for disease molecular mechanism research and drug discovery.X-ray crystallography is one of the m... 详细信息

关键词： selenium- and tellurium-derivatization nucleic acids X-ray crystal structure and function studies.

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