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Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

BIOCELL 2022年第1期46卷 247-261页

作者： JELAN MOFEED MOHAMED DEYAB ABDELNASER MOHAMED MAHMOUD MOUSTAFA sally negm EMAD EL-BILAWY Faculty of Science King Salman International UniversitySinaiEgypt Faculty of Fish Resources Suez UniversitySuezEgypt Faculty of Science Damietta UniversityDamiettaEgypt Department of Biology College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQenaEgypt Life Sciences Department Faculty of Science and ArtKing Khalid UniversityMahayel Aseer 61421Saudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkia44516Egypt

Microbial infections cause complicated health influences along with bad economic *** the present investigation,three dominant seaweeds namely,Amphiroa anceps,Corallina officinalis and Sargassum filipendula were collected from different Egyptian sites at the Red Sea and Mediterranean Sea during autumn *** extracts of the three algae were screened for their antibacterial activity against three pathogenic bacteria Salmonella typhiimurium,Staphylococcus aureus and Escherichia coli,in addition to in vitro antiviral activity against Rotavirus(RV),and Coxsackie virus B3(CVB3)that cause severe diseases in *** extract of ***,*** and *** inhibit *** cells by 57.1%,85.7%,and 91.4%,*** highest level of concentration of the *** extract(100μg/mL)inhibits 100%of Staphylococcus aureus cells followed by *** and *** extract which inhibit 82.5%and 75%of ***,the highest concentration of *** extract inhibits *** by 80%.The extract of *** exhibited a high antiviral effect against RV infection with TI=22 and virus titers lessened by 2.75 log TCID_(50) followed by extractions of *** with TI=18.3 and virus titers reduced by 2.5 log TCID_(50).Against CVB3 infection,the extract of *** causes the highest antiviral activity with TI=15 and reduce the viral titers by 2.5 log TCID_(50),followed by extractions of *** with TI=8.8 and inhibition of virus titers by 1.75 log TCID_(50).Extract of *** displayed the lowest antiviral effects against RV and CVB3 infection with TI=2.4 and 1.4,*** obtained results clarified that the extract of three marine seaweeds maintains a potent antimicrobial activity,making them a future promising source of new antimicrobial drugs.

关键词： Seaweeds Antimicrobial Antibacterial Cytotoxicity Antiviral

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Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source,Talaromyces purpureogenus KJ584844

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BIOCELL 2021年第1期45卷 119-127页

作者： sally negm MOHAMMAD EL-METWALLY WESAM ELDIN SABER SAHAR ABO-NEIMA MAHMOUD MOUSTAFA ATTALLA EL-KOTT Life Sciences Department College of Science and literature Mahyel AseerKing Khalid UniversityAbhaSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkiaEgypt Department of Botany and Microbiology Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Physics Department Faculty of ScienceDamanhour UniversityDamanhourEgypt Department of Biology College of ScienceKing Khalid University9004AbhaSaudi Arabia Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQenaEgypt Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt

The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this *** proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation *** interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state *** optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g *** Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase *** nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the *** findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase *** approach could be extended to modulate the productivity and activity of similar biomolecules.

关键词： Experimental design Helianthus tuberosus proteolysis solid-state fermentation

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Secondary antiviral metabolites from fungi with special reference to coronaviruses

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BIOCELL 2022年第8期46卷 1979-1988页

作者： MOHAMED SALEM MOHAMMAD EL-METWALLY WESAMELDIN SABER sally negm ATTALLA EL-KOTT YASSER MAZROUA ABEER MAKHLOUF MAHMOUD MOUSTAFA Department of Chemistry Faculty of Science and ArtKing Khalid UniversityMohailAssirSaudi Arabia Department of Chemistry Faculty of ScienceAl-Azhar UniversityCairoEgypt Botany and Microbiology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Life Sciences Department College of Science and ArtKing Khalid UniversityMohailAseerSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkiaEgypt Department of Biology College of ScienceKing Khalid UniversityAbhaSaudi Arabia Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Department of Business Administration Community CollegeKing Khalid UniversityAbhaSaudi Arabia Department of Agricultural Economics Faculty of AgricultureTanta UniversityTantaEgypt Department of Botany Faculty of AgricultureMenoufia UniversitySheben El-KomMenoufiaEgypt Botany and Microbiology Department Faculty of ScienceSouth Valley UniversityQenaEgypt

Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various *** of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals,viral contagions are amongst the most causative diseases affecting people *** exemplify a massive source of bioactive molecules as,many fungal secondary metabolities like Oxoglyantrypine,Carneic acid F,Scedapin C,Asteltoxin E,Phomanolide,Norquinadoline A and Quinadoline B have antiviral *** review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially Coronaviruses moreover several pieces of literature pointed out that many clusters of fungi in different biotopes are waiting to be exploited.

关键词： COVID-19 SARS-CoV-2 Antibiotics Virus Fungi

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