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Non-random tissue distribution of human na ve umbilical cord matrix stem cells

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World Journal of stem Cells 2011年第4期3卷 34-42页

作者： Dharmendra Kumar Maurya Chiyo Doi Marla Pyle Raja shekar Rachakatla Duane Davis Masaaki tamura Deryl Troyer Department of Anatomy and Physiology College of Veterinary Medicine Kansas State University Department of Animal sciences and Industry Kansas State University

AIM: To determine the tissue and temporal distribution of human umbilical cord matrix stem (hUCMs) cells in severe combined immunodeficiency (sCID) mice. METHODs: For studying the localization of hUCMs cells, tritiated thymidine-labeled hUCMs cells were injected in sCID mice and tissue distribution was quantitatively determined using a liquid scintillation counter at days 1, 3, 7 and 14. Furthermore, an immunofluorescence detection technique was employed in which anti-human mitochondrial antibody was used to identify hUCMs cells in mouse tissues. In order to visualize the distribution of transplanted hUCMs cells in H&E stained tissue sections, India Black ink 4415 was used to label the hUCMs cells. REsULTs: When tritiated thymidine-labeled hUCMs cells were injected systemically (iv) in female sCID mice, the lung was the major site of accumulation at 24 h after transplantation. With time, the cells migrated to other tissues, and on day three, the spleen, stomach, and small and large intestines were the major accumulation sites. On day seven, a relatively large amount of radioactivity was detected in the adrenal gland, uterus, spleen, lung, and digestive tract. In addition, labeled cells had crossed the blood brain barrier by day 1. CONCLUsION: These results indicate that peripherally injected hUCMs cells distribute quantitatively in a tissue-specific manner throughout the body.

关键词： Human umbilical cord matrix stem cells Immunohistochemistry India black ink loaded cells Radio-labeled transplanted cells Tissue distribution

精力的文学评论为执行 laparoscopic colorectal 外科采购原料

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World Journal of Gastrointestinal surgery 2012年第1期4卷 1-8页

作者： Tsukasa Hotta Katsunari Takifuji shozo Yokoyama Kenji Matsuda Takashi Higashiguchi Toshiji Tominaga Yoshimasa Oku Takashi Watanabe Toru Nasu Tadamichi Hashimoto Koichi tamura Junji Ieda Naoyuki Yamamoto Hiromitsu Iwamoto Hiroki Yamaue second Department of surgery Wakayama Medical UniversitySchool of Medicine

Laparoscopic surgery for colorectal disease has become widespread as a minimally invasive *** is important because the increasing availability of new devices allows us to perform procedures with a reduced length of surgery and decreased blood *** herein report the results of a literature review of energy sources for laparoscopic colorectal surgery,focused especially on 6 studies comparing ultrasonic coagulating shears(UCs) and other *** also describe our laparoscopic dissection techniques using UCs for colorectal *** short-term outcomes of surgeries using UCs and Ligasure for laparoscopiccolorectal surgery were superior to conventional *** authors have reported that the length of surgery or blood loss when Ligasure was used for laparoscopic colorectal surgery is less than when UCs was *** the other hand,a recent study demonstrated that there were no significant differences between the short-term outcomes of UCs and Ligasure for laparoscopic colorectal *** is therefore suggested that the choice of technique used should be made according to the surgeon’s *** also describe our laparoscopic dissection techniques using UCs(Harmonic ACE) for colorectal cancer with regard to the retroperitoneum dissection,dissection technique,dissection technique around the feeding artery,and various other dissection *** therefore review the outcomes of using various energy sources for laparoscopic colorectal surgery and describe our laparoscopic dissection techniques with UCs(Harmonic ACE) for colorectal cancer.

关键词： Laparoscopic surgery Ultrasonic coagulating shears Harmonic scalpel Ligasure Colon and rectum Dissection technique

降低体部CT成像金属弹簧圈伪影:新的单能降低金属伪影算法的效果

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国际医学放射学杂志 2016年第4期 451-451页

作者： M.Kidoh D.Utsunomiya O.Ikeda Y.tamura s.Oda Y.Funama 戴群瑶 Department of Diagnostic Radiology Faculty of Life Sciences Kumamoto University

摘要目的评价单能降低金属伪影(sEMAR)算法减少体部CT成像中金属弹簧圈伪影的效果。方法对30例[男10例,女20例,平均年龄(67.9±11)岁]体内有金属弹簧圈的病人行CTA检查。非sEMAR影像只进行迭代法重建,而sEMAR影像进行迭代法及sEMAR算法... 详细信息

摘要目的评价单能降低金属伪影(sEMAR)算法减少体部CT成像中金属弹簧圈伪影的效果。方法对30例[男10例,女20例,平均年龄(67.9±11)岁]体内有金属弹簧圈的病人行CTA检查。非sEMAR影像只进行迭代法重建,而sEMAR影像进行迭代法及sEMAR算法重建。比较非sEMAR

关键词：弹簧圈伪影体部算法 CT

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利用控轧提高si-Mn弹簧钢的冷轧性能

上海金属 1992年第2期14卷 30-34页

作者：殷匠吕以撒田村今男上海第五钢铁厂日本应用科学研究所

si-Mn弹簧钢带钢在热轧过程中,用控轧法使发生动态再结晶,加以降低终轧温度使奥氏体转变温度提高,钢带以4～7K/s的速率自然冷却时,能获得细致的片状珠光体和少量铁索体组织,这种轧态组织能经受70％以上的冷轧压缩率,因此可大大地简化工... 详细信息

si-Mn弹簧钢带钢在热轧过程中,用控轧法使发生动态再结晶,加以降低终轧温度使奥氏体转变温度提高,钢带以4～7K/s的速率自然冷却时,能获得细致的片状珠光体和少量铁索体组织,这种轧态组织能经受70％以上的冷轧压缩率,因此可大大地简化工艺。用这种带钢制作的弹簧,性能大幅度提高。

关键词：带钢控轧弹簧钢冷轧性能

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基于GET技术的混合厌氧发酵产甲烷研究

生物资源 2019年第5期41卷 450-457页

作者：王弯陈芳清黄永文田村广人刘杨赟官守鹏湖北省三峡地区生态保护与治理国际联合研究中心湖北宜昌443002 湖北正江环保科技有限公司湖北宜昌443002 日本名城大学农学部日本名古屋468-8502

GET是一种国外新研发的在田间利用秸秆进行甲烷生产的技术。为了推动该技术在我国的实际应用,本研究以稻草与牛粪为发酵原料,水稻田土壤作为基底,采用正交试验法,测试了温度、发酵基质配比(稻草∶牛粪)和秸秆腐熟剂浓度对混合厌氧发酵... 详细信息

GET是一种国外新研发的在田间利用秸秆进行甲烷生产的技术。为了推动该技术在我国的实际应用,本研究以稻草与牛粪为发酵原料,水稻田土壤作为基底,采用正交试验法,测试了温度、发酵基质配比(稻草∶牛粪)和秸秆腐熟剂浓度对混合厌氧发酵产气量的影响。结果表明:各处理因子的产气动态呈“s”型增长模式,但在快速增长时间和增长幅度上有一定区别。30℃和40℃处理的累积产气量显著高于10℃和20℃处理的累积产气量;发酵基质配比以1∶1处理的累积产气量最大,分别比1∶0(对照)、1∶2和1∶3配比高34.12%、20.39%和21.78%;秸秆腐熟剂浓度的产气效果在实验范围内随着浓度的增加而增加。厌氧发酵过程中各实验因子下挥发性脂肪酸(VFAs)含量和pH在20 d左右分别有一个高峰和低谷值且都有较大差异,各因子主要是通过在这个时期对厌氧发酵进程的影响而对整个产气量产生影响。综合分析显示稻草厌氧发酵产生物甲烷的最优组合为:温度30℃、发酵基质配比1∶1、秸秆腐熟剂浓度8%。

关键词： GET技术厌氧发酵正交试验方差分析

强化与标准血压控制对痴呆的影响:随机临床试验

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中华高血压杂志 2019年第4期27卷 367-367页

作者：刘青叶鹏 Williamson JD Pajewski NM Auchus AP Bryan RN Chelune G Cheung AK Cleveland ML Coker LH Crowe MG Cushman WC Cutler JA Davatzikos C Desiderio L Erus G Fine LJ Gaussoin sA Harris D Hsieh MK Johnson KC Kimmel PL tamura MK Launer LJ Lerner AJ Lewis CE Martindale-Adams J Moy Cs Nasrallah IM Nichols LO Oparil s Ogrocki PK Rahman M Rapp sR Reboussin DM Rocco MV sachs BC sink KM still CH supiano MA snyder JK Wadley VG Walker J Weiner DE Whelton PK Wilson VM Woolard N Wright JT Jr Wright CB sPRINT MIND investigators for thesPRINT research group

目前还没有有效的治疗方法来降低轻度认知障碍和痴呆的风险。该文旨在探讨强化血压控制对老年痴呆风险的影响。研究人员在美国和波多黎各102个地点对年龄≥50岁有高血压但无糖尿病或脑卒中史的成人进行随机临床试验。于2010年11月8日开... 详细信息

目前还没有有效的治疗方法来降低轻度认知障碍和痴呆的风险。该文旨在探讨强化血压控制对老年痴呆风险的影响。研究人员在美国和波多黎各102个地点对年龄≥50岁有高血压但无糖尿病或脑卒中史的成人进行随机临床试验。于2010年11月8日开始,于2015年8月20日提前停止,获得其主要观察指标(复合心血管事件)和全因死亡率的数据。认知观察指标随访的最后日期为2018年7月22日。

关键词：随机临床试验老年痴呆血压控制轻度认知障碍标准心血管事件治疗方法研究人员

Nature：大规模研究揭示生命所必需的基因

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现代生物医学进展 2016年第31期16卷 I0002-I0003页

作者： Dickinson, Mary E. Flenniken, Ann M. Ji, Xiao Teboul, Lydia Wong, Michael D. White, Jacqueline K. Meehan, Terrence F. Weninger, Wolfgang J. Westerberg, Henrik Adissu, Hibret Baker, Candice N. Bower, Lynette Brown, James M. Caddle, L. Brianna Chiani, Francesco Clary, Dave Cleak, James Daly, Mark J. Denegre, James M. Doe, Brendan Dolan, Mary E. Edie, sarah M. Fuchs, Helmut Gailus-Durner, Valerie Galli, Antonella Gambadoro, Alessia Gallegos, Juan Guo, shiying Horner, Neil R. Hsu, Chih-Wei Johnson, sara J. Kalaga, sowmya Keith, Lance C. Lanoue, Louise Lawson, Thomas N. Lek, Monkol Mark, Manuel Arschall, susan M. Mason, Jeremy McElwee, Melissa L. Newbigging, susan Nutter, Lauryl M. J. Peterson, Kevin A. Ramirez-solis, Ramiro Rowland, Douglas J. Ryder, Edward samocha, Kaitlin E. seavitt, John R. selloum, Mohammed szoke-Kovacs, Zsombor tamura, Masaru Trainor, Amanda G. Tudose, Ilinca Wakana, shigeharu Warren, Jonathan Wendling, Olivia West, David B. Wong, Leeyean Yoshiki, Atsushi MacArthur, Daniel G. Tocchini-Valentini, Glauco P. Gao, Xiang Flicek, Paul Bradley, Allan skarnes, William C. Justice, Monica J. Parkinson, Helen E. Moore, Mark Wells, sara Braun, Robert E. svenson, Karen L. de Angelis, Martin Hrabe Herault, Yann Mohun, Tim Mallon, Ann-Marie Henkelman, R. Mark Brown, steve D. M. Adams, David J. Lloyd, K. C. Kent McKerlie, Colin Beaudet, Arthur L. Bucan, Maja Murray, stephen A. Dept Mol Physiol & Biophys Houston TX 77030 USA Toronto Ctr Phenogen Toronto ON M5T 3H7 Canada Mt sinai Hosp Toronto ON M5G 1X5 Canada Univ Penn Perelman Sch Med Genom & Computat Biol Program Philadelphia PA 19104 USA Med Res Council Harwell Mammalian Genet Unit Harwell OX11 0RD Oxon England Mary Lyon Ctr Harwell OX11 0RD Oxon England Hosp sick Children Mouse Imaging Ctr Toronto ON M5T 3H7 Canada Wellcome Trust sanger Inst Wellcome Trust Genome Campus Cambridge CB10 1SA England European Bioinformat Inst European Mol Biol Lab Wellcome Trust Genome Campus Cambridge CB10 1SD England Med Univ Vienna Ctr Anat & Cell Biol A-1090 Vienna Austria Hosp sick Children 555 Univ Ave Toronto ON M5G 1X8 Canada Jackson Lab 600 Main St Bar Harbor ME 04609 USA Univ Calif Davis Mouse Biol Program Davis CA 95618 USA Italian Natl Res Council CNR Inst Cell Biol & Neurobiol Monterotondo Mouse Clin I-00015 Monterotondo Italy Massachusetts Gen Hosp Analyt & Translat Genet Unit Boston MA 02114 USA Broad Inst MIT & Harvard Program Med & Populat Genet Cambridge MA 02142 USA German Res Ctr Environm Hlth Helmholtz Zentrum Munchen Inst Expt Genet D-85764 Neuherberg Germany German Mouse Clin D-85764 Neuherberg Germany Baylor Coll Med Dept Mol & Human Genet Houston TX 77030 USA Nanjing Univ Nanjing Biomed Res Inst Collaborat Innovat Ctr Genet & Dev SKL Pharmaceut Biotechnol & Model Anim Res Ctr Nanjing 210061 Jiangsu Peoples R China Univ strasbourg ICS Infrastruct Natl PHENOMIN F-67404 Illkirch Graffenstaden France Univ strasbourg INSERM CNRS IGBMC F-67404 Illkirch Graffenstaden France RIKEN BioResource Ctr Tsukuba Ibaraki 3050074 Japan Childrens Hosp Oakland Res Inst Oakland CA 94609 USA IMPC San Anselmo CA 94960 USA Tech Univ Munich Sch Life Sci Weihenstephan Chair Expt Genet D-81675 Freising Weihenstephan Germany German Ctr Diabet Res DZD D-85764 Neuherberg Germany Francis Crick Inst Mill Hill Lab Mill Hill London NW1 1AT England Univ Penn Perlman

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期... 详细信息

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期刊上，论文标题为“High—throughput discovery of novel developmental phenotypes”。

关键词：动物基因组生命哺乳动物研究人员人类疾病医学院