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Developing herbicide resistant sri-lankan rice(Oryza sativa L.)varieties:An application of self Organizing Map

Information Processing in Agriculture 2017年第2期4卷 140-149页

作者： E.M.s.I.Ekanayaka s.R.Weerakoon s.somaratne O.V.D.s.J.Weerasena Department of Botany The Open University of Sri LankaP.O.Box 21NawalaSri Lanka Institutes of Biochemistry Molecular Biology and BiotechnologyUniversity of ColomboSri Lanka

Application of high concentrations of post-emergent broad spectrum systemic herbicide,glyphosate is prevalently used to control rice weeds in Asian countries including sri *** target movements of glyphosate adversely affect growth of the rice plant reducing the *** herbicide resistance(HR)in cultivated rice is a novel approach to enhance selectivity and crop *** on induced HR in sri Lankan rice varieties are limited and studies are required to include HR rice in a cropping *** Methyl sulfonate(EMs),a chemical mutagen is used for functional *** present study is an attempt of raising HR rice lines through conventional breeding methods.A suitable glyphosate concentration was determined by a preliminary study using five rice varieties(Bg300,Bg352,At362,Bg379-2 and H4)and varying glyphosate concentrations(0.25 g/l,0.5 g/l,1 g/l,2 g/l and 3 g/l).Resistance percentage50%was arbitrary considered as resistant *** Randomized Design with three replicates was used in the *** characters were recorded for the survived *** varieties(Bg359,At362,Bw364,Ld365,Bg366,Bg369,Bg379-2,Bg403,Bg454“Pachcha perumal”,“Kalu heenati”and“Kurulu thuda”)showed natural resistance to glyphosate and fourteen varieties have increased resistance after mutagenesis by EMs(s0-First generation).HR resistance percentage of s1(second generation)plants was similar to s0 plants indicating HR was transferred to new *** statistics was supplemented with self-Organizing Maps(sOM)to visualize variation of agro-morphological characters under *** result proved that EMs application is an effective method in breeding new rice germplasm with HR and sOM is an important tool for visualizing the multi-dimensional dataset in lower dimensions.

关键词： Ethyl Methyl sulfonate Glyphosate Herbicide resistance Oryza sativa self-Organizing Map

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Estimating Out-Crossing Rate of Bg 379-2 Using Morphological Markers and Confirmation by Molecular Markers

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Rice science 2012年第2期19卷 166-168页

作者： L.H.M.Y.K.somaratne A.s.M.T.ABAYAWICKRAMA I.P.WICKRAMAsINGHE W.L.G.sAMARAsINGHE Department of Agricultural Biology Faculty of AgricultureUniversity of PeradeniyaSri Lanka Rice Research and Development Institute BatalagodaIbbagamuwaSri Lanka

Rice is largely self-fertilized and accordingly a field population of rice is completely composed of near homozygotes. Due to the emergence of off-types, homozygosity will be affected. With the time, this will cause the reduction of genetic purity in some rice varieties. One of the reasons has been suspected to be the high out-crossing frequencies of such varieties. studies were conducted at the Rice Research and Development Institute, Batalagoda, sri Lanka to estimate the out-crossing rate of Bg 379-2, a variety having the problem of maintaining genetic purity. Bg 379-2 was allowed to out-cross with Bg 450 and the number of out- crossed plants were counted using dominant morphological markers such as short-round grain and purple culm of pollen donor. A molecular confirmation of out-crossing was also performed using sequence tagged site （sTs） molecular marker pTA248. The variety Bg 379-2 showed a potential out-crossing rate of 3.41% and an average out-crossing rate of 1.29% using dominant morphological markers. Polymorphism was cleady detected between parents and out-crossed plants as well as selfed plants of Bg 379-2 using their banding patterns. A similar study can be performed to determine the out-crossing rates of other varieties which show high percentage of off-types in the population for the better understanding of the breeding behavior of the varieties.

关键词： rice genetic purity out-crossing marker polymorphism

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斯里兰卡当地芥菜(Brassica juncea)的鉴定及其营养品质的改良(英文)

斯里兰卡当地芥菜(Brassica juncea)的鉴定及其营养品质的改良(英...

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第十二届国际油菜大会

作者： s R Weerakoon M C M Iqbal s.somaratne P K D Peiris W s R Wimalasuriya Department of Botany The Open UniversityNawalaSri Lanka Plant Reproductive Biology Division Institute of Fundamental StudiesKandySri Lanka

芥菜(Brassica juncea)作为油料作物生长在印地安次大陆有几百年历史,然而在斯里兰卡,芥菜生产相对较晚。它被广泛用作为调味品并且油是用于Ayurvedic药中。在斯里兰卡有60份芥菜资源,然而它们的遗传多样性和相互关系还没有研究。本研... 详细信息

芥菜(Brassica juncea)作为油料作物生长在印地安次大陆有几百年历史,然而在斯里兰卡,芥菜生产相对较晚。它被广泛用作为调味品并且油是用于Ayurvedic药中。在斯里兰卡有60份芥菜资源,然而它们的遗传多样性和相互关系还没有研究。本研究的目的是评估当地芥菜的遗传差异,通过对其农艺性状的数学分析来界定和证实其遗传差异和农业的优势近源种。筛选了30份芥菜资源并测定35个农艺性状。数据采用不同的多参数统计程序:聚类分析、主要组分分析原理(PCA)和判别式功能分析(DFA)。聚类、PCA和DFA的表明结果显示芥菜变种有不同的组型。因而,怀疑用形态特征界定芥菜变种是否适合。因此,本研究认为考虑其他的信息源如生化证据和分子标记来证明芥菜变种。当地芥菜的不受欢迎的脂肪酸(芥酸)的含量高,受欢迎的脂肪酸(芥酸)的含量低。高水平芥酸对心肌有不良影响,商业油菜canola品种因其含单不饱和油而在营养上受亲睐。6种澳大利亚商业春性canola品种和6个当地芥菜品种相互杂交,已成功培育F1代杂种。通过使用改良的Lichter medium(1982)培养基进行胚培养获得了F1再生植株,这样克服了萌发后屏障。所有杂交F1代种子用气相色谱进行脂肪酸的分析显示其适量的芥酸(18%1%)而对照芥菜为44%6%,甘蓝型油菜为0.2%.5%。F1杂种油酸提高(33%1%)而对照芥菜(7%%)和甘蓝型油菜(43%7%)。本研究清楚说明了一些特性如高水平油酸和低水平芥酸是可能从canola转移到芥菜中的,并通过种间杂交生产新的具改良的脂肪酸组分的芥菜品系。

关键词： mustard accessions morphology fatty acid profile CA PCA DFA interspecific hybridization sri Lanka

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